scholarly journals Broom: Application for non-redundant storage of High Throughput Sequencing data

2018 ◽  
Author(s):  
Levent Albayrak ◽  
Kamil Khanipov ◽  
George Golovko ◽  
Yuriy Fofanov
Keyword(s):  
Data Storage ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Data Generation ◽  
Sequencing Data ◽  
High Throughput Sequencing Data ◽  
Sequencing Quality ◽  
Redundant Storage ◽  
Recent Trends ◽  
The Cost

AbstractMotivationThe data generation capabilities of High Throughput Sequencing (HTS) instruments have exponentially increased over the last few years, while the cost of sequencing has dramatically decreased allowing this technology to become widely used in biomedical studies. For small labs and individual researchers, however, storage and transfer of large amounts of HTS data present a significant challenge. The recent trends in increased sequencing quality and genome coverage can be used to reconsider HTS data storage strategies.ResultsWe present Broom, a stand-alone application designed to select and store only high-quality sequencing reads at extremely high compression rates. Written in C++, the application accepts single and paired-end reads in FASTQ and FASTA formats and decompresses data in FASTA format.AvailabilityC++ code available at https://scsb.utmb.edu/labgroups/fofanov/[email protected]

scholarly journals Broom: application for non-redundant storage of high throughput sequencing data

2018 ◽  
Vol 35 (1) ◽  
pp. 143-145 ◽  
Author(s):  
Levent Albayrak ◽  
Kamil Khanipov ◽  
George Golovko ◽  
Yuriy Fofanov
Keyword(s):  
High Throughput ◽  
High Throughput Sequencing ◽  
Sequencing Data ◽  
High Throughput Sequencing Data ◽  
Redundant Storage

scholarly journals Great differences in performance and outcome of high-throughput sequencing data analysis platforms for fungal metabarcoding

MycoKeys ◽  
2018 ◽  
Vol 39 ◽  
pp. 29-40 ◽  
Author(s):  
Sten Anslan ◽  
R. Henrik Nilsson ◽  
Christian Wurzbacher ◽  
Petr Baldrian ◽  
Leho Tedersoo ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Sequencing ◽  
Computation Time ◽  
Potential Effect ◽  
Data Sets ◽  
Sequencing Data ◽  
Operational Taxonomic Units ◽  
High Throughput Sequencing Data ◽  
Recent Developments

Along with recent developments in high-throughput sequencing (HTS) technologies and thus fast accumulation of HTS data, there has been a growing need and interest for developing tools for HTS data processing and communication. In particular, a number of bioinformatics tools have been designed for analysing metabarcoding data, each with specific features, assumptions and outputs. To evaluate the potential effect of the application of different bioinformatics workflow on the results, we compared the performance of different analysis platforms on two contrasting high-throughput sequencing data sets. Our analysis revealed that the computation time, quality of error filtering and hence output of specific bioinformatics process largely depends on the platform used. Our results show that none of the bioinformatics workflows appears to perfectly filter out the accumulated errors and generate Operational Taxonomic Units, although PipeCraft, LotuS and PIPITS perform better than QIIME2 and Galaxy for the tested fungal amplicon dataset. We conclude that the output of each platform requires manual validation of the OTUs by examining the taxonomy assignment values.

scholarly journals Improvements and impacts of GRCh38 human reference on high throughput sequencing data analysis

Genomics ◽  
2017 ◽  
Vol 109 (2) ◽  
pp. 83-90 ◽  
Author(s):  
Yan Guo ◽  
Yulin Dai ◽  
Hui Yu ◽  
Shilin Zhao ◽  
David C. Samuels ◽  
...  
Keyword(s):  
Data Analysis ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Sequencing Data ◽  
High Throughput Sequencing Data ◽  
Sequencing Data Analysis

scholarly journals HTSeq - A Python framework to work with high-throughput sequencing data

2014 ◽  
Author(s):  
Simon Anders ◽  
Paul Theodor Pyl ◽  
Wolfgang Huber
Keyword(s):  
High Throughput ◽  
High Throughput Sequencing ◽  
Rapid Development ◽  
Differential Expression Analysis ◽  
Rna Seq ◽  
Sequencing Data ◽  
Standard Work ◽  
Data Formats ◽  
High Throughput Sequencing Data ◽  
Python Package

Motivation: A large choice of tools exists for many standard tasks in the analysis of high-throughput sequencing (HTS) data. However, once a project deviates from standard work flows, custom scripts are needed. Results: We present HTSeq, a Python library to facilitate the rapid development of such scripts. HTSeq offers parsers for many common data formats in HTS projects, as well as classes to represent data such as genomic coordinates, sequences, sequencing reads, alignments, gene model information, variant calls, and provides data structures that allow for querying via genomic coordinates. We also present htseq-count, a tool developed with HTSeq that preprocesses RNA-Seq data for differential expression analysis by counting the overlap of reads with genes. Availability: HTSeq is released as open-source software under the GNU General Public Licence and available from http://www-huber.embl.de/HTSeq or from the Python Package Index, https://pypi.python.org/pypi/HTSeq

scholarly journals hypeR: an R package for geneset enrichment workflows

2019 ◽  
Author(s):  
Anthony Federico ◽  
Stefano Monti
Keyword(s):  
High Throughput ◽  
High Throughput Sequencing ◽  
R Package ◽  
Use Cases ◽  
Sequencing Data ◽  
Wide Audience ◽  
Popular Method ◽  
High Throughput Sequencing Data ◽  
One Stop ◽  
Recent Version

Abstract Summary Geneset enrichment is a popular method for annotating high-throughput sequencing data. Existing tools fall short in providing the flexibility to tackle the varied challenges researchers face in such analyses, particularly when analyzing many signatures across multiple experiments. We present a comprehensive R package for geneset enrichment workflows that offers multiple enrichment, visualization, and sharing methods in addition to novel features such as hierarchical geneset analysis and built-in markdown reporting. hypeR is a one-stop solution to performing geneset enrichment for a wide audience and range of use cases. Availability and implementation The most recent version of the package is available at https://github.com/montilab/hypeR. Contact [email protected] or [email protected]

Sign in / Sign up

Export Citation Format

Share Document