scholarly journals Longitudinal tracking of Plasmodium falciparum clones in complex infections by amplicon deep sequencing

2018 ◽  
Author(s):  
Anita Lerch ◽  
Cristian Koepfli ◽  
Natalie E. Hofmann ◽  
Johanna H. Kattenberg ◽  
Anna Rosanas-Urgell ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Papua New Guinea ◽  
Deep Sequencing ◽  
Polymorphic Marker ◽  
Additional Parameter ◽  
Force Of Infection ◽  
Individual Clone ◽  
Longitudinal Tracking ◽  
Infection Dynamics ◽  
Higher Sensitivity

AbstractBackgroundLongitudinal tracking of individual Plasmodium falciparum strains in multi-clonal infections is essential for investigating infection dynamics of malaria. The traditional genotyping techniques did not permit tracking changes in individual clone density during persistent natural infections. Amplicon deep sequencing (Amp-Seq) offers a tool to address this knowledge gap.MethodsThe sensitivity of Amp-Seq for relative quantification of clones was investigated using three molecular markers, ama1-D2, ama1-D3, and cpmp. Amp-Seq and length-polymorphism based genotyping were compared for their performance in following minority clones in longitudinal samples from Papua New Guinea.ResultsAmp-Seq markers were superior to length-polymorphic marker msp2 in detecting minority clones (sensitivity Amp-Seq: 95%, msp2: 85%). Multiplicity of infection (MOI) by Amp-Seq was 2.32 versus 1.73 for msp2. The higher sensitivity had no effect on estimates of force of infection because missed minority clones were detected in preceding or succeeding bleeds. Individual clone densities were tracked longitudinally by Amp-Seq despite MOI>1, thus providing an additional parameter for investigating malaria infection dynamics.ConclusionAmp-Seq based genotyping of longitudinal samples improves detection of minority clones and estimates of MOI. Amp-Seq permits tracking of clone density over time to study clone competition or the dynamics of specific, i.e. resistance-associated genotypes.

DRUG RESISTANT STRAINS OF PLASMODIUM FALCIPARUM IN PAPUA NEW GUINEA

The Lancet ◽  
1981 ◽  
Vol 317 (8216) ◽  
pp. 386
Author(s):  
Brian Darlow ◽  
Helena Vrbova ◽  
John Stace ◽  
Peter Heywood ◽  
Michael Alpers
Keyword(s):  
Plasmodium Falciparum ◽  
Papua New Guinea ◽  
New Guinea ◽  
Drug Resistant ◽  
Resistant Strains ◽  
Drug Resistant Strains

Role of pfmdr1 mutations on chloroquine resistance in Plasmodium falciparum isolates with pfcrt K76T from Papua New Guinea

Acta Tropica ◽  
2006 ◽  
Vol 98 (2) ◽  
pp. 137-144 ◽  
Author(s):  
Toshihiro Mita ◽  
Akira Kaneko ◽  
Francis Hombhanje ◽  
Ilomo Hwaihwanje ◽  
Nobuyuki Takahashi ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Papua New Guinea ◽  
New Guinea ◽  
Chloroquine Resistance

scholarly journals Matched Placental and Circulating Plasmodium falciparum Parasites are Genetically Homologous at the var2csa ID1-DBL2X Locus by Deep Sequencing

2018 ◽  
Vol 98 (1) ◽  
pp. 77-82 ◽  
Author(s):  
Andreea Waltmann ◽  
Achille Massougbodji ◽  
Steven R. Meshnick ◽  
Jonathan J. Juliano ◽  
Christian M. Parobek ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Deep Sequencing

scholarly journals Sex-based differences in clearance of chronic Plasmodium falciparum infection

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Jessica Briggs ◽  
Noam Teyssier ◽  
Joaniter I Nankabirwa ◽  
John Rek ◽  
Prasanna Jagannathan ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Host Response ◽  
Behavioral Risk ◽  
Biological Sex ◽  
Force Of Infection ◽  
Host Parasite Interactions ◽  
Infection Event ◽  
Asymptomatic Infections ◽  
Host Parasite ◽  
Eastern Uganda

Multiple studies have reported a male bias in incidence and/or prevalence of malaria infection in males compared to females. To test the hypothesis that sex-based differences in host-parasite interactions affect the epidemiology of malaria, we intensively followed Plasmodium falciparum infections in a cohort in a malaria endemic area of eastern Uganda and estimated both force of infection (FOI) and rate of clearance using amplicon deep-sequencing. We found no evidence of differences in behavioral risk factors, incidence of malaria, or FOI by sex. In contrast, females cleared asymptomatic infections at a faster rate than males (hazard ratio [HR]=1.82, 95% CI 1.20 to 2.75 by clone and HR = 2.07, 95% CI 1.24 to 3.47 by infection event) in multivariate models adjusted for age, timing of infection onset, and parasite density. These findings implicate biological sex-based differences as an important factor in the host response to this globally important pathogen.

scholarly journals Increase in the proportion of Plasmodium falciparum with kelch13 C580Y mutation and decline in pfcrt and pfmdr1 mutant alleles in Papua New Guinea

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Naoko Yoshida ◽  
Masato Yamauchi ◽  
Ryosuke Morikawa ◽  
Francis Hombhanje ◽  
Toshihiro Mita
Keyword(s):  
Plasmodium Falciparum ◽  
Papua New Guinea ◽  
New Guinea ◽  
Therapeutic Effects ◽  
Cross Sectional Survey ◽  
Cross Sectional ◽  
In The Wild ◽  
Nationwide Surveillance ◽  
Greater Mekong Subregion ◽  
C580y Mutation

Abstract Background The C580Y mutation in the Plasmodium falciparum kelch13 gene is the most commonly observed variant in artemisinin-resistant isolates in the Greater Mekong Subregion (GMS). Until 2017, it had not been identified outside the GMS, except for Guyana/Amazonia. In 2017, three parasites carrying the C580Y mutation were identified in Papua New Guinea (PNG). As the C580Y allele rapidly spread in the GMS, there is concern that this mutant is now spreading in PNG. Methods In 2020, a cross-sectional survey was conducted at two clinics in Wewak, PNG. Symptomatic patients infected with P. falciparum were treated with artemether plus lumefantrine following a national treatment policy. Blood samples were obtained before treatment, and polymorphisms in kelch13, pfcrt, and pfmdr1 were determined. Parasite positivity was examined on day 3. The results were compared with those of previous studies conducted in 2002, 2003, and 2016–2018. Results A total of 94 patients were included in this analysis. The proportion of C580Y was significantly increased (2.2% in 2017, 5.7% in 2018, and 6.4% in 2020; p = 4.2 × 10–3). A significant upward trend was observed in the wild-type proportion for pfcrt (1.9% in 2016 to 46.7% in 2020; p = 8.9 × 10–16) and pfmdr1 (59.5% in 2016 to 91.4% in 2020; p = 2.3 × 10–6). Among 27 patients successfully followed on day 3, including three with C580Y infections, none showed positive parasitaemia. Conclusions Under the conditions of significant increases in pfcrt K76 and pfmdr1 N86 alleles in PNG, the increase in kelch13 C580Y mutants may be a warning indicator of the emergence of parasites resistant to the currently used first-line treatment regimen of artemether plus lumefantrine. Therefore, nationwide surveillance of molecular markers for drug resistance and assessment of its therapeutic effects are important.

scholarly journals Detection of low-density Plasmodium falciparum infections using amplicon deep sequencing

2019 ◽  
Vol 18 (1) ◽  
Author(s):  
Angela M. Early ◽  
Rachel F. Daniels ◽  
Timothy M. Farrell ◽  
Jonna Grimsby ◽  
Sarah K. Volkman ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Deep Sequencing ◽  
Low Density
Sign in / Sign up

Export Citation Format

Share Document