scholarly journals TAF-ChIP: An ultra-low input approach for genome wide chromatin immunoprecipitation assay

2018 ◽  
Author(s):  
Junaid Akhtar ◽  
Piyush More ◽  
Steffen Albrecht ◽  
Federico Marini ◽  
Waldemar Kaiser ◽  
...  
Keyword(s):  
Chromatin Immunoprecipitation ◽  
Signal To Noise Ratio ◽  
High Signal ◽  
Signal To Noise ◽  
Chromatin Immunoprecipitation Assay ◽  
High Agreement ◽  
Genome Wide ◽  
Sequencing Method ◽  
Noise Ratio ◽  
Generation Sequencing

AbstractChromatin immunoprecipitation (ChIP) followed by next generation sequencing (ChIP-Seq) is powerful technique to study transcriptional regulation. However, the requirement of millions of cells to generate results with high signal-to-noise ratio precludes it in the study of small cell populations. Here, we present a Tagmentation-Assisted Fragmentation ChIP (TAF-ChIP) and sequencing method to generate high-quality results from low cell numbers. The data obtained from the TAF-ChIP approach is amenable to standard tools for ChIP-Seq analysis, owing to its high signal-to-noise ratio. The epigenetic profiles from TAF-ChIP approach showed high agreement with conventional ChIP-Seq datasets, thereby underlining the utility of this approach.

scholarly journals TAF-ChIP: an ultra-low input approach for genome-wide chromatin immunoprecipitation assay

2019 ◽  
Vol 2 (4) ◽  
pp. e201900318 ◽  
Author(s):  
Junaid Akhtar ◽  
Piyush More ◽  
Steffen Albrecht ◽  
Federico Marini ◽  
Waldemar Kaiser ◽  
...  
Keyword(s):  
Chromatin Immunoprecipitation ◽  
Signal To Noise Ratio ◽  
High Signal ◽  
Signal To Noise ◽  
Chromatin Immunoprecipitation Assay ◽  
High Agreement ◽  
Genome Wide ◽  
Sequencing Method ◽  
Noise Ratio ◽  
Generation Sequencing

Chromatin immunoprecipitation (ChIP) followed by next generation sequencing (ChIP-Seq) is a powerful technique to study transcriptional regulation. However, the requirement of millions of cells to generate results with high signal-to-noise ratio precludes it in the study of small cell populations. Here, we present a tagmentation-assisted fragmentation ChIP (TAF-ChIP) and sequencing method to generate high-quality histone profiles from low cell numbers. The data obtained from the TAF-ChIP approach are amenable to standard tools for ChIP-Seq analysis, owing to its high signal-to-noise ratio. The epigenetic profiles from TAF-ChIP approach showed high agreement with conventional ChIP-Seq datasets, thereby underlining the utility of this approach.

Narrow-frequency sharp-angular filters using all-dielectric cascaded meta-gratings

Nanophotonics ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 3443-3450 ◽  
Author(s):  
Wei-Nan Liu ◽  
Rui Chen ◽  
Wei-Yi Shi ◽  
Ke-Bo Zeng ◽  
Fu-Li Zhao ◽  
...  
Keyword(s):  
Thin Film ◽  
Signal To Noise Ratio ◽  
Incident Angle ◽  
Design Strategy ◽  
Transmission Peak ◽  
High Signal ◽  
Waveguide Array ◽  
Signal To Noise ◽  
Center Wavelength ◽  
Noise Ratio

AbstractSelective transmission or filtering always responds to either frequency or incident angle, so as hardly to maximize signal-to-noise ratio in communication, detection and sensing. Here, we propose compact meta-filters of narrow-frequency sharp-angular transmission peak along with broad omnidirectional reflection sidebands, in all-dielectric cascaded subwavelength meta-gratings. The inherent collective resonance of waveguide-array modes and thin film approximation of meta-grating are employed as the design strategy. A unity transmission peak, locating at the incident angle of 44.4° and the center wavelength of 1550 nm, is demonstrated in a silicon meta-filter consisting of two-layer silicon rectangular meta-grating. These findings provide possibilities in cascaded meta-gratings spectroscopic design and alternative utilities for high signal-to-noise ratio applications in focus-free spatial filtering and anti-noise systems in telecommunications.

scholarly journals Low-dose, phase-contrast mammography with high signal-to-noise ratio

2016 ◽  
Vol 7 (2) ◽  
pp. 381 ◽  
Author(s):  
Lukas B. Gromann ◽  
Dirk Bequé ◽  
Kai Scherer ◽  
Konstantin Willer ◽  
Lorenz Birnbacher ◽  
...  
Keyword(s):  
Phase Contrast ◽  
Low Dose ◽  
Signal To Noise Ratio ◽  
High Signal ◽  
Signal To Noise ◽  
Noise Ratio

Research on LIFT Technology for 3D Seismic Denoising in Coalfield

2014 ◽  
Vol 556-562 ◽  
pp. 6328-6331
Author(s):  
Su Zhen Shi ◽  
Yi Chen Zhao ◽  
Li Biao Yang ◽  
Yao Tang ◽  
Juan Li
Keyword(s):  
Signal To Noise Ratio ◽  
High Signal ◽  
3D Seismic ◽  
Signal To Noise ◽  
Denoising Method ◽  
Seismic Processing ◽  
Work Area ◽  
Noise Data ◽  
Noise Ratio ◽  
Effective Signal

The LIFT technology has applied in process of denoising to ensure the imaging precision of minor faults and structure in 3D coalfield seismic processing. The paper focused on the denoising process in two study areas where the LIFT technology is used. The separation of signal and noise is done firstly. Then denoising would be done in the noise data. The Data of weak effective signal that is from the noise data could be blended with the original effective signal to reconstruct the denoising data, so the result which has high signal-to-noise ratio and preserved amplitude is acquired. Thus the fact shows that LIFT is an effective denoising method for 3D seismic in coalfield and could be used widely in other work area.

High signal-to-noise ratio quantum well bolometer materials

2006 ◽  
Author(s):  
Stanley Wissmar ◽  
Linda Höglund ◽  
Jan Andersson ◽  
Christian Vieider ◽  
Susan Savage ◽  
...  
Keyword(s):  
Quantum Well ◽  
Signal To Noise Ratio ◽  
High Signal ◽  
Signal To Noise ◽  
Noise Ratio

EE-RS and PA for untrusted relay network at high signal-to-noise ratio regime

2016 ◽  
Vol 10 (16) ◽  
pp. 2143-2148 ◽  
Author(s):  
Houman Zarrabi ◽  
Ali Kuhestani ◽  
Majid Moradikia
Keyword(s):  
Signal To Noise Ratio ◽  
Relay Network ◽  
High Signal ◽  
Signal To Noise ◽  
Noise Ratio ◽  
Untrusted Relay
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