scholarly journals An ultralight head-mounted camera system integrates detailed behavioral monitoring with multichannel electrophysiology in freely moving mice

2018 ◽  
Author(s):  
Arne F. Meyer ◽  
Jasper Poort ◽  
John O’Keefe ◽  
Maneesh Sahani ◽  
Jennifer F. Linden
Keyword(s):  
Neural Circuit ◽  
Model Organism ◽  
Neural Recording ◽  
Head Movements ◽  
Head Orientation ◽  
Eye Position ◽  
Neural Basis ◽  
Camera System ◽  
Recording Technology ◽  
Behavioral Monitoring

SummaryBreakthroughs in understanding the neural basis of natural behavior require neural recording and intervention to be paired with high-fidelity multimodal behavioral monitoring. An extensive genetic toolkit for neural circuit dissection, and well-developed neural recording technology, make the mouse a powerful model organism for systems neuroscience. However, methods for high-bandwidth acquisition of behavioral signals in mice remain limited to fixed-position cameras and other off-animal devices, complicating the monitoring of animals freely engaged in natural behaviors. Here, we report the development of an ultralight head-mounted camera system combined with head-movement sensors to simultaneously monitor eye position, pupil dilation, whisking, and pinna movements along with head motion in unrestrained, freely behaving mice. The power of the combined technology is demonstrated by observations linking eye position to head orientation; whisking to non-tactile stimulation; and, in electrophysiological experiments, visual cortical activity to volitional head movements.

scholarly journals COMPARATIVE MEASUREMENTS OF HEAD ANGULAR MOVEMENTS USING A CAMERA SYSTEM AND A GYROSCOPE SYSTEM

2014 ◽  
Vol 54 (4) ◽  
pp. 295-300 ◽  
Author(s):  
Vladimir Socha ◽  
Patrik Kutilek ◽  
Ondrej Cakrt ◽  
Rudolf Cerny
Keyword(s):  
Head Movements ◽  
Head Orientation ◽  
Quiet Stance ◽  
Body Segment ◽  
Mean Velocity ◽  
Camera System ◽  
Rehabilitation Process ◽  
Gyroscope System ◽  
The Mean ◽  
2D Data

Assessments of body-segment angular movements are very important in the rehabilitation process. Head angular movements are measured and analyzed for use in studies of stability and posture. However, there is no methodology for assessing angular movements of the head, and it has not been verified whether data measured by fundamentally different MoCap systems will lead to the same results. In this study, we used a camera system and a 3DOF orientation tracker placed on the subject’s head, and measured inclination (roll) and flexion (pitch) during quiet stance. The total length and the mean velocity of the traces of the pitch versus roll plots were used to measure and analyze head orientation. Using these methods, we are able to model the distribution of the measured 2D data, and to evaluate stability and posture. The results show that the total lengths and the mean velocities related to the 3DOF orientation tracker do not differ significantly from the total lengths and the mean velocities of traces related to the IR medical camera. We also found that the systems are not interchangeable, and that the same type of system must be used each time. The designed methods can be used for studies not only of head movements but also of movements of other segments of the human body, and can be used to compare other types of MoCap systems, depending on the requirements for a specific rehabilitation examination.

scholarly journals Mechanisms of punctuated vision in fly flight

2020 ◽  
Author(s):  
Benjamin Cellini ◽  
Wael Salem ◽  
Jean-Michel Mongeau
Keyword(s):  
Active Vision ◽  
Causal Link ◽  
Head Movements ◽  
Flight Simulator ◽  
Head Orientation ◽  
Eye Position ◽  
Elastic Recoil ◽  
Motor Tasks ◽  
Gaze Stabilization ◽  
Complex Motor

ABSTRACTTo guide locomotion, animals control their gaze via movements of their eyes, head, and/or body, but how the nervous system controls gaze during complex motor tasks remains elusive. Notably, eye movements are constrained by anatomical limits, which requires resetting eye position. By studying tethered, flying flies (Drosophila) in a virtual reality flight simulator, we show that ballistic head movements (saccades) reset eye position, are stereotyped and leverage elastic recoil of the neck joint, enabling mechanically assisted redirection of gaze. Head reset saccades were of proprioceptive origin and interrupted smooth movements for as little as 50 ms, enabling punctuated, near-continuous gaze stabilization. Wing saccades were modulated by head orientation, establishing a causal link between neck signals and execution of body saccades. Furthermore, we demonstrate that head movements are gated by behavioral state. We propose a control architecture for biological and bio-inspired active vision systems with limits in sensor range of motion.

scholarly journals Performance of microvascular anastomosis with a new robotic visualization system: proof of concept

2021 ◽  
Author(s):  
F. Boehm ◽  
P. J. Schuler ◽  
R. Riepl ◽  
L. Schild ◽  
T. K. Hoffmann ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Body Position ◽  
Head Movements ◽  
Camera System ◽  
Visualization System ◽  
Head Mounted Display ◽  
General Evaluation ◽  
Surgical Field ◽  
Technical Instructions

AbstractMicrovascular procedures require visual magnification of the surgical field, e.g. by a microscope. This can be accompanied by an unergonomic posture with musculoskeletal pain or long-term degenerative changes as the eye is bound to the ocular throughout the whole procedure. The presented study describes the advantages and drawbacks of a 3D exoscope camera system. The RoboticScope®-system (BHS Technologies®, Innsbruck, Austria) features a high-resolution 3D-camera that is placed over the surgical field and a head-mounted-display (HMD) that the camera pictures are transferred to. A motion sensor in the HMD allows for hands-free change of the exoscope position via head movements. For general evaluation of the system functions coronary artery anastomoses of ex-vivo pig hearts were performed. Second, the system was evaluated for anastomosis of a radial-forearm-free-flap in a clinical setting/in vivo. The system positioning was possible entirely hands-free using head movements. Camera control was intuitive; visualization of the operation site was adequate and independent from head or body position. Besides technical instructions of the providing company, there was no special surgical training of the surgeons or involved staff upfront performing the procedures necessary. An ergonomic assessment questionnaire showed a favorable ergonomic position in comparison to surgery with a microscope. The outcome of the operated patient was good. There were no intra- or postoperative complications. The exoscope facilitates a change of head and body position without losing focus of the operation site and an ergonomic working position. Repeated applications have to clarify if the system benefits in clinical routine.

scholarly journals Direct measurement of vagal tone in rats does not show correlation to HRV

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Joseph T. Marmerstein ◽  
Grant A. McCallum ◽  
Dominique M. Durand
Keyword(s):  
Heart Failure ◽  
Heart Rate ◽  
Heart Rate Variability ◽  
Neural Activity ◽  
Vagal Tone ◽  
Neural Recording ◽  
The Body ◽  
Autonomic Nerve ◽  
Vagal Activity ◽  
Recording Technology

AbstractThe vagus nerve is the largest autonomic nerve, innervating nearly every organ in the body. “Vagal tone” is a clinical measure believed to indicate overall levels of vagal activity, but is measured indirectly through the heart rate variability (HRV). Abnormal HRV has been associated with many severe conditions such as diabetes, heart failure, and hypertension. However, vagal tone has never been directly measured, leading to disagreements in its interpretation and influencing the effectiveness of vagal therapies. Using custom carbon nanotube yarn electrodes, we were able to chronically record neural activity from the left cervical vagus in both anesthetized and non-anesthetized rats. Here we show that tonic vagal activity does not correlate with common HRV metrics with or without anesthesia. Although we found that average vagal activity is increased during inspiration compared to expiration, this respiratory-linked signal was not correlated with HRV either. These results represent a clear advance in neural recording technology but also point to the need for a re-interpretation of the link between HRV and “vagal tone”.

scholarly journals Neural Basis of Visually Guided Head Movements Studied With fMRI

2003 ◽  
Vol 89 (5) ◽  
pp. 2516-2527 ◽  
Author(s):  
Laurent Petit ◽  
Michael S. Beauchamp
Keyword(s):  
Eye Movements ◽  
Head Movement ◽  
Brain Activity ◽  
Posterior Part ◽  
Vestibular Stimulation ◽  
Head Movements ◽  
Oculomotor Control ◽  
Imaging Studies ◽  
Neural Basis ◽  
Temporoparietal Junction

We used event-related fMRI to measure brain activity while subjects performed saccadic eye, head, and gaze movements to visually presented targets. Two distinct patterns of response were observed. One set of areas was equally active during eye, head, and gaze movements and consisted of the superior and inferior subdivisions of the frontal eye fields, the supplementary eye field, the intraparietal sulcus, the precuneus, area MT in the lateral occipital sulcus and subcortically in basal ganglia, thalamus, and the superior colliculus. These areas have been previously observed in functional imaging studies of human eye movements, suggesting that a common set of brain areas subserves both oculomotor and head movement control in humans, consistent with data from single-unit recording and microstimulation studies in nonhuman primates that have described overlapping eye- and head-movement representations in oculomotor control areas. A second set of areas was active during head and gaze movements but not during eye movements. This set of areas included the posterior part of the planum temporale and the cortex at the temporoparietal junction, known as the parieto-insular vestibular cortex (PIVC). Activity in PIVC has been observed during imaging studies of invasive vestibular stimulation, and we confirm its role in processing the vestibular cues accompanying natural head movements. Our findings demonstrate that fMRI can be used to study the neural basis of head movements and show that areas that control eye movements also control head movements. In addition, we provide the first evidence for brain activity associated with vestibular input produced by natural head movements as opposed to invasive caloric or galvanic vestibular stimulation.

scholarly journals Effects of behavioural activation on the neural circuit related to intrinsic motivation

BJPsych Open ◽  
2018 ◽  
Vol 4 (5) ◽  
pp. 317-323 ◽  
Author(s):  
Asako Mori ◽  
Yasumasa Okamoto ◽  
Go Okada ◽  
Koki Takagaki ◽  
Masahiro Takamura ◽  
...  
Keyword(s):  
Intrinsic Motivation ◽  
Neural Circuit ◽  
Intervention Group ◽  
Behavioural Activation ◽  
Functional Magnetic Resonance ◽  
Resonance Imaging ◽  
Neural Basis ◽  
Efficient Treatment ◽  
The Right ◽  
Magnetic Resonance Imaging Scanning

BackgroundBehavioural activation is an efficient treatment for depression and can improve intrinsic motivation. Previous studies have revealed that the frontostriatal circuit is involved in intrinsic motivation; however, there are no data on how behavioural activation affects the frontostriatal circuit.AimsWe aimed to investigate behavioural activation-related changes in the frontostriatal circuit.MethodFifty-nine individuals with subthreshold depression were randomly assigned to either the intervention or non-intervention group. The intervention group received five weekly behavioural activation sessions. The participants underwent functional magnetic resonance imaging scanning on two separate occasions while performing a stopwatch task based on intrinsic motivation. We investigated changes in neural activity and functional connectivity after behavioural activation.ResultsAfter behavioural activation, the intervention group had increased activation and connectivity in the frontostriatal region compared with the non-intervention group. The increased activation in the right middle frontal gyrus was correlated with an improvement of subjective sensitivity to environmental rewards.ConclusionsBehavioural activation-related changes to the frontostriatal circuit advance our understanding of psychotherapy-induced improvements in the neural basis of intrinsic motivation.Declaration of interestNone.

scholarly journals A visual circuit uses complementary mechanisms to support transient and sustained pupil constriction

eLife ◽  
2016 ◽  
Vol 5 ◽  
Author(s):  
William Thomas Keenan ◽  
Alan C Rupp ◽  
Rachel A Ross ◽  
Preethi Somasundaram ◽  
Suja Hiriyanna ◽  
...  
Keyword(s):  
Neural Coding ◽  
Pupil Size ◽  
Neural Circuit ◽  
Ganglion Cells ◽  
Retinal Ganglion ◽  
Neural Basis ◽  
Behavioral Dynamics ◽  
Pupil Constriction ◽  
Sustained Responses ◽  
Stable Control

Rapid and stable control of pupil size in response to light is critical for vision, but the neural coding mechanisms remain unclear. Here, we investigated the neural basis of pupil control by monitoring pupil size across time while manipulating each photoreceptor input or neurotransmitter output of intrinsically photosensitive retinal ganglion cells (ipRGCs), a critical relay in the control of pupil size. We show that transient and sustained pupil responses are mediated by distinct photoreceptors and neurotransmitters. Transient responses utilize input from rod photoreceptors and output by the classical neurotransmitter glutamate, but adapt within minutes. In contrast, sustained responses are dominated by non-conventional signaling mechanisms: melanopsin phototransduction in ipRGCs and output by the neuropeptide PACAP, which provide stable pupil maintenance across the day. These results highlight a temporal switch in the coding mechanisms of a neural circuit to support proper behavioral dynamics.

scholarly journals Hyperspectral imaging of retinal microvascular anatomy

2015 ◽  
Vol 2 (1) ◽  
pp. 139 ◽  
Author(s):  
Amir H. Kashani ◽  
Mark Wong ◽  
Nicole Koulisis ◽  
Chein-I Chang ◽  
Gabriel Martin ◽  
...  
Keyword(s):  
Hyperspectral Image ◽  
Model Organism ◽  
Science Research ◽  
Tomographic Imaging ◽  
Diagnostic Methods ◽  
Camera System ◽  
Computed Tomographic ◽  
Non Invasive ◽  
Computed Tomographic Imaging

Background: Hyperspectral image processing has been applied to many aspects of astronomical and earth science research. Furthermore, advances in computed tomographic imaging spectroscopy and diffraction grating design have allowed biological applications for non-invasive tissue analysis. Herein, we describe a hyperspectral computed tomographic imaging spectroscope (HCTIS) that provides high spatial, spectral and temporal resolution ideal for imaging biological tissue in vivo. Methods: We demonstrate proof-of-principle application of the HCTIS by imaging and mapping the microvascular anatomy of the retina of a model organism (rabbit) in vivo. The imaging procedure allows rapid and dense spectral sampling, is non-toxic, non-invasive, and easily adaptable to a commercially available fundus camera system. Results: HCTIS provides highly co-registered temporal, spatial and spectral data with resolution capable of reconstructing the fine vascular tree of the rabbit retina in vivo. Conclusions: We show that HCTIS allows for reliable and reproducible tissue classification and detection using signature discriminant analysis. Future applications of this system may provide promising diagnostic methods for diseases of many tissues. 

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