scholarly journals The Mitochondrial DNA Control Region might have useful Diagnostic and Prognostic Biomarkers for Thyroid Tumors

2018 ◽  
Author(s):  
Rifat Bircan ◽  
Hülya Iliksu Gözü ◽  
Ulu Esra ◽  
Şükran Sarikaya ◽  
Aylin Ege Gül ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Somatic Mutations ◽  
Thyroid Nodules ◽  
Turkish Population ◽  
Thyroid Tumor ◽  
Thyroid Tumors ◽  
Benign Thyroid Nodules ◽  
Cold Thyroid Nodules ◽  
Benign Thyroid

AbstractBackgroundIt is currently present in the literature that mitochondrial DNA (mtDNA) defects are associated with a great number of diseases including cancers. The role of mitochondrial DNA (mtDNA) variations in the development of thyroid cancer is a highly controversial topic. In this study, we investigated the role of mt-DNA control region (CR) variations in thyroid tumor progression and the influence of mtDNA haplogroups on susceptibility to thyroid tumors.Material & methodFor this purpose, totally 108 hot thyroid nodules (HTNs), 95 cold thyroid nodules (CTNs), 48 papillary thyroid carcinoma (PTC) samples with their surrounding tissues and 104 healthy control subject’s blood samples were screened for entire mtDNA CR variations by using Sanger sequencing. The obtained DNA sequences were anaysed with the mistomaster, a web-based bioinformatics tool.ResultsMtDNA haplogroup U was significantly associated with susceptibility to benign and malign thyroid entities on the other hand J haplogroup was associated with a protective role for benign thyroid nodules. Besides, 8 SNPs (T146C, G185A, C194T, C295T, G16129A, T16304C, A16343G and T16362C) in mtDNA CR region were associated with the occurrence of benign and malign thyroid nodules in Turkish population. By contrast with the healthy Turkish population and HTNs, frequency of C7 repeats in D310 polycytosine sequence was found higher in cold thyroid nodules and PTC samples. Beside this, the frequency of somatic mutations in mtMSI regions including T16189C and D514 CA dinucleotide repeats were found higher in PTC samples than the benign thyroid nodules. Conversely, the frequency of somatic mutations in D310 was detected higher in HTNs than CTNs and PTCs.ConclusionmtDNA D310 instability do not play a role in tumorogenesis of the PTC but the results indicates that it might be used as a diagnostic clonal expansion biomarker for premalignant thyroid tumor cells. Beside this, D514 CA instability might be used as prognostic biomarker in PTCs. Also, we showed that somatic mutation rate is less frequent in more aggressive tumors when we examined micro- and macro carcinomas as well as BRAFV600E mutation.

scholarly journals The Mitochondrial DNA Control Region Might Have Useful Diagnostic and Prognostic Biomarkers for Thyroid Tumors

2019 ◽  
Vol 127 (07) ◽  
pp. 423-436
Author(s):  
Rıfat Bircan ◽  
Hülya Ilıksu Gözü ◽  
Esra Ulu ◽  
Şükran Sarıkaya ◽  
Aylin Ege Gül ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Control Region ◽  
Somatic Mutations ◽  
Thyroid Nodules ◽  
Turkish Population ◽  
Thyroid Tumor ◽  
Thyroid Tumors ◽  
Mt Dna ◽  
Benign Thyroid

AbstractThe literature suggests that mitochondrial DNA (mtDNA) defects are associated with a large number of diseases including cancers. The role of mtDNA variations in thyroid cancer is a highly controversial topic. Therefore, we investigated the role of mt-DNA control region (CR) variations in thyroid tumor progression and the influence of mtDNA haplogroups on susceptibility to thyroid tumors. For this purpose, in total, 108 hot thyroid nodules (HTNs), 95 cold thyroid nodules (CTNs), 48 papillary thyroid carcinoma (PTC) samples with their surrounding tissues and 104 healthy control subjects’ blood samples were screened for all mtDNA CR variations using Sanger sequencing. We found that MtDNA haplogroup U was significantly associated with susceptibility to benign thyroid entities. In addition, eight single nucleotide polymorphisms (SNPs) (T146C, G185A, C194T, C295T, G16129A, T16304C, A16343G and T16362C) in the mtDNA CR were associated with the occurrence of benign and malign thyroid nodules in the Turkish population. As compared with samples taken from a healthy Turkish population and HTNs, the frequency of C7 repeats in D310 polycytosine sequence was found to be higher in CTNs and the PTC samples. In addition, the frequency of somatic mutations in mtMSI regions including T16189C and D514 CA dinucleotide repeats were found to be higher in PTC samples than benign thyroid nodules. Conversely, the frequency of somatic mutations in D310 was found to be higher in HTNs than CTNs and PTCs. In conclusion, mtDNA D310 instability does not play a role in the tumorigenesis of PTC but the results indicate that it might be used as a diagnostic clonal expansion biomarker for premalignant thyroid tumor cells. In addition, D514 CA instability might be considered as a prognostic biomarker for benign to malign transformation in thyroid tumors.

What Is the Role of Radiofrequency Ablation for Benign Thyroid Nodules?

2021 ◽  
Author(s):  
Peter Horwich ◽  
Brent A. Chang ◽  
Ameya A. Asarkar ◽  
Gregory W. Randolph ◽  
Cherie‐Ann O. Nathan
Keyword(s):  
Radiofrequency Ablation ◽  
Thyroid Nodules ◽  
Benign Thyroid Nodules ◽  
Benign Thyroid

scholarly journals Benign Aspirates on Follow-Up FNA May Be Enough in Patients with Initial Atypia of Undetermined Significance/Follicular Lesion of Undetermined Significance

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Ga Ram Kim ◽  
Jung Hyun Yoon ◽  
Eun-Kyung Kim ◽  
Hee Jung Moon ◽  
Jin Young Kwak
Keyword(s):  
Thyroid Nodules ◽  
Surgical Excision ◽  
Needle Aspiration ◽  
Risk Of Malignancy ◽  
Benign Nodules ◽  
Benign Thyroid Nodules ◽  
Benign Thyroid ◽  
Undetermined Significance

Background.Management of thyroid nodules with benign aspirates following atypia of undetermined significance/follicular lesion of undetermined significance (AUS/FLUS) is not well established. We reviewed the risk of malignancy and the role of ultrasound (US) features among thyroid nodules with benign results following initial AUS/FLUS diagnoses.Methods.From December 2009 to February 2011, a total of 114 nodules in 114 patients diagnosed as benign on follow-up fine-needle aspiration (FNA) after AUS/FLUS results were included in our study. Eight among 114 nodules were confirmed pathologically and 106 were clinically observed by a follow-up FNA or US. Suspicious US features were defined as markedly hypoechogenicity, irregular or microlobulated margin, presence of microcalcifications, and taller than wide shape.Results.There were 110 (96.5%) benign nodules and 4 (3.5%) malignant nodules. Two (4.8%) among 42 nodules without suspicious US features and 2 (2.8%) out of 72 nodules with suspicious US features were confirmed as malignancy, but there were no significant associations between the malignancy rate and US features (P=0.625).Conclusion.Clinical follow-up instead of surgical excision or continuous repeat FNA may be enough for benign thyroid nodules after AUS/FLUS. The role of US features might be insignificant in the management of these nodules.

scholarly journals Use of Radiofrequency Ablation in Benign Thyroid Nodules: A Literature Review and Updates

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Kai-Pun Wong ◽  
Brian Hung-Hin Lang
Keyword(s):  
Radiofrequency Ablation ◽  
Laser Ablation ◽  
Thermal Ablation ◽  
Thyroid Nodules ◽  
Kidney Tumors ◽  
Ethanol Ablation ◽  
Efficient Treatment ◽  
Benign Thyroid Nodules ◽  
Cold Thyroid Nodules ◽  
Benign Thyroid

Successful thermal ablation using radiofrequency has been reported in various tumors including liver or kidney tumors. Nonsurgical minimally invasive ablative therapy such as radiofrequency ablation (RFA) has been reported to be a safe and efficient treatment option in managing symptomatic cold thyroid nodules or hyperfunctioning thyroid nodules. Pressure and cosmetic symptoms have been shown to be significantly improved both in the short and long terms after RFA. For hyperfunctioning thyroid nodules, RFA is indicated for whom surgery or radioiodine are not indicated or ineffective or for those who refuse surgery or radio-iodine. Improvement of thyroid function with decreased need for antithyroid medications has been reported. Complication rate is relatively low. By reviewing the current literature, we reported its efficacy and complications and compared the efficacy of RFA relative to other ablative options such as ethanol ablation and laser ablation.

scholarly journals Role of Duplex Power Doppler Ultrasound in Differentiation between Malignant and Benign Thyroid Nodules

2010 ◽  
Vol 11 (6) ◽  
pp. 594 ◽  
Author(s):  
Oktay Algin ◽  
Efnan Algin ◽  
Gokhan Gokalp ◽  
Gokhan Ocakoğlu ◽  
Cüneyt Erdoğan ◽  
...  
Keyword(s):  
Doppler Ultrasound ◽  
Thyroid Nodules ◽  
Power Doppler ◽  
Power Doppler Ultrasound ◽  
Benign Thyroid Nodules ◽  
Benign Thyroid

scholarly journals Role of cytological characteristics of benign thyroid nodules on effectiveness of their treatment with levothyroxine

2013 ◽  
Vol 6 ◽  
pp. 1083-1089
Author(s):  
Mustafa Cesu ◽  
Mehtap Akcil ◽  
Sibel Ertek ◽  
Rifat Emral ◽  
Safak Bulut ◽  
...  
Keyword(s):  
Thyroid Nodules ◽  
Cytological Characteristics ◽  
Benign Thyroid Nodules ◽  
Benign Thyroid

Evidence-Based Assessment of the Role of Ultrasonography in the Management of Benign Thyroid Nodules

2008 ◽  
Vol 32 (7) ◽  
pp. 1253-1263 ◽  
Author(s):  
Lilah F. Morris ◽  
Nagesh Ragavendra ◽  
Michael W. Yeh
Keyword(s):  
Thyroid Nodules ◽  
Evidence Based ◽  
Benign Thyroid Nodules ◽  
Benign Thyroid
Sign in / Sign up

Export Citation Format

Share Document