scholarly journals Histone H3 T11 phosphorylation by Sch9 and CK2 regulates lifespan by controlling the nutritional stress response

2018 ◽  
Author(s):  
Seunghee Oh ◽  
Tamaki Suganuma ◽  
Madelaine M. Gogol ◽  
Jerry L. Workman
Keyword(s):  
Stress Response ◽  
Signaling Pathways ◽  
Stress Responses ◽  
Histone H3 ◽  
Nutritional Stress ◽  
Stress Conditions ◽  
Metabolic Status ◽  
Chromatin Regulation ◽  
Nutrient Signaling ◽  
Altered Metabolism

AbstractUpon nutritional stress, the metabolic status of cells is changed by nutrient signaling pathways to ensure survival. Altered metabolism by nutrient signaling pathways has been suggested to influence cellular lifespan. However, it remains unclear how chromatin regulation is involved in this process. Here, we found that histone H3 threonine 11 phosphorylation (H3pT11) functions as a marker for nutritional stress and aging. Sch9 and CK2 kinases cooperatively regulate H3pT11 under stress conditions. Importantly, H3pT11 defective mutants prolonged chronological lifespan by altering nutritional stress responses. Thus, the phosphorylation of H3T11 by Sch9 and CK2 engages a nutritional stress response to chromatin in the regulation of lifespan.

scholarly journals Histone H3 threonine 11 phosphorylation by Sch9 and CK2 regulates chronological lifespan by controlling the nutritional stress response

eLife ◽  
2018 ◽  
Vol 7 ◽  
Author(s):  
Seunghee Oh ◽  
Tamaki Suganuma ◽  
Madelaine M Gogol ◽  
Jerry L Workman
Keyword(s):  
Stress Response ◽  
Signaling Pathways ◽  
Stress Responses ◽  
Histone H3 ◽  
Nutritional Stress ◽  
Stress Conditions ◽  
Metabolic Status ◽  
Chronological Lifespan ◽  
Nutrient Signaling ◽  
Altered Metabolism

Upon nutritional stress, the metabolic status of cells is changed by nutrient signaling pathways to ensure survival. Altered metabolism by nutrient signaling pathways has been suggested to influence cellular lifespan. However, it remains unclear how chromatin regulation is involved in this process. Here, we found that histone H3 threonine 11 phosphorylation (H3pT11) functions as a marker for nutritional stress and aging. Sch9 and CK2 kinases cooperatively regulate H3pT11 under stress conditions. Importantly, H3pT11 defective mutants prolonged chronological lifespan (CLS) by altering nutritional stress responses. Thus, the phosphorylation of H3T11 by Sch9 and CK2 links a nutritional stress response to chromatin in the regulation of CLS.

scholarly journals The unpredictability of prolonged activation of stress response pathways

2015 ◽  
Vol 209 (6) ◽  
pp. 781-787 ◽  
Author(s):  
Lilian T. Lamech ◽  
Cole M. Haynes
Keyword(s):  
Stress Response ◽  
Signaling Pathways ◽  
Stress Responses ◽  
Degenerative Diseases ◽  
Response To Stress ◽  
Cellular Compartments

In response to stress, cellular compartments activate signaling pathways that mediate transcriptional programs to promote survival and reestablish homeostasis. Manipulation of the magnitude and duration of the activation of stress responses has been proposed as a strategy to prevent or repair the damage associated with aging or degenerative diseases. However, as these pathways likely evolved to respond specifically to transient perturbations, the unpredictability of prolonged activation should be considered.

scholarly journals Specific functions for Mediator complex subunits from different modules in the transcriptional response of Arabidopsis thaliana to abiotic stress

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Tim Crawford ◽  
Fazeelat Karamat ◽  
Nóra Lehotai ◽  
Matilda Rentoft ◽  
Jeanette Blomberg ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Abiotic Stress ◽  
Signaling Pathways ◽  
Stress Responses ◽  
Target Genes ◽  
Biochemical Characterization ◽  
Transcriptional Response ◽  
Stress Conditions ◽  
Important Player ◽  
Adverse Environmental Conditions

AbstractAdverse environmental conditions are detrimental to plant growth and development. Acclimation to abiotic stress conditions involves activation of signaling pathways which often results in changes in gene expression via networks of transcription factors (TFs). Mediator is a highly conserved co-regulator complex and an essential component of the transcriptional machinery in eukaryotes. Some Mediator subunits have been implicated in stress-responsive signaling pathways; however, much remains unknown regarding the role of plant Mediator in abiotic stress responses. Here, we use RNA-seq to analyze the transcriptional response of Arabidopsis thaliana to heat, cold and salt stress conditions. We identify a set of common abiotic stress regulons and describe the sequential and combinatorial nature of TFs involved in their transcriptional regulation. Furthermore, we identify stress-specific roles for the Mediator subunits MED9, MED16, MED18 and CDK8, and putative TFs connecting them to different stress signaling pathways. Our data also indicate different modes of action for subunits or modules of Mediator at the same gene loci, including a co-repressor function for MED16 prior to stress. These results illuminate a poorly understood but important player in the transcriptional response of plants to abiotic stress and identify target genes and mechanisms as a prelude to further biochemical characterization.

scholarly journals Yeast Nuak1 phosphorylates histone H3 threonine 11 in low glucose stress conditions by the cooperation of AMPK and CK2 signaling

2020 ◽  
Author(s):  
Seunghee Oh ◽  
Jaehyoun Lee ◽  
Selene K. Swanson ◽  
Laurence Florens ◽  
Michael P. Washburn ◽  
...  
Keyword(s):  
Nuclear Localization ◽  
Histone H3 ◽  
Nutritional Stress ◽  
Chromatin Regulation ◽  
Available Nutrients ◽  
Low Glucose ◽  
Living Organisms ◽  
Histone Kinase ◽  
Transcription Program ◽  
External Glucose

AbstractChanges in available nutrients are inevitable events for most living organisms. Upon nutritional stress, several signaling pathways cooperate to change the transcription program through chromatin regulation to rewire cellular metabolism. In budding yeast, histone H3 threonine 11 phosphorylation (H3pT11) acts as a marker of low glucose stress and regulates the transcription of nutritional stress responsive genes. Understanding how this histone modification ‘senses’ external glucose changes remains elusive. Here, we show that Tda1, the yeast orthologue of human Nuak1, is a direct kinase for H3pT11 upon low glucose stress. Yeast AMPK directly phosphorylates Tda1 to govern Tda1 activity, while CK2 regulates Tda1 nuclear localization. Collectively, AMPK and CK2 signaling converge on histone kinase Tda1 to link external low glucose stress to chromatin regulation.

scholarly journals Yeast Nuak1 phosphorylates histone H3 threonine 11 in low glucose stress by the cooperation of AMPK and CK2 signaling

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Seunghee Oh ◽  
Jaehyoun Lee ◽  
Selene K Swanson ◽  
Laurence Florens ◽  
Michael P Washburn ◽  
...  
Keyword(s):  
Histone H3 ◽  
Nutritional Stress ◽  
Chromatin Regulation ◽  
Available Nutrients ◽  
Low Glucose ◽  
Living Organisms ◽  
Histone Kinase ◽  
Amp Activated Protein Kinase ◽  
Transcription Program ◽  
External Glucose

Changes in available nutrients are inevitable events for most living organisms. Upon nutritional stress, several signaling pathways cooperate to change the transcription program through chromatin regulation to rewire cellular metabolism. In budding yeast, histone H3 threonine 11 phosphorylation (H3pT11) acts as a marker of low glucose stress and regulates the transcription of nutritional stress-responsive genes. Understanding how this histone modification ‘senses’ external glucose changes remains elusive. Here, we show that Tda1, the yeast ortholog of human Nuak1, is a direct kinase for H3pT11 upon low glucose stress. Yeast AMP-activated protein kinase (AMPK) directly phosphorylates Tda1 to govern Tda1 activity, while CK2 regulates Tda1 nuclear localization. Collectively, AMPK and CK2 signaling converge on histone kinase Tda1 to link external low glucose stress to chromatin regulation.

scholarly journals PpSARK Regulates Moss Senescence and Salt Tolerance through ABA Related Pathway

2018 ◽  
Vol 19 (9) ◽  
pp. 2609 ◽  
Author(s):  
Ping Li ◽  
Hong Yang ◽  
Gaojing Liu ◽  
Wenzhang Ma ◽  
Chuanhong Li ◽  
...  
Keyword(s):  
Salt Stress ◽  
Stress Response ◽  
Salt Tolerance ◽  
Stress Responses ◽  
Physcomitrella Patens ◽  
Stress Conditions ◽  
Negative Regulator ◽  
Salt Stress Response ◽  
Functional Roles ◽  
Related Pathway

Senescence-associated receptor-like kinase (SARK) family members in Arabidopsis, soybean, and rice are known to be positive regulators of leaf senescence. In the meantime, SARKs are extensively involved in stress response. However, their function and underlying molecular mechanism in stress responses in moss are not well known. Here, we investigated functional roles of SARK isolated from Physcomitrella patens (PpSARK) in salt stress response and senescence. PpSARK transcripts significantly accumulated under NaCl and abscisic acid (ABA) treatments, with higher expression in the moss gametophyte stage. Insertional gain-of-function mutants of PpSARK (PpSARKg) were more tolerant to salt stress and ABA than wild type (WT), whereas senescence of mutants was delayed during the protonema stage. Expression of stress-responsive genes in the ABA related pathway, such as PpABI3, PpABI5, PpPP2C, and PpLEA were significantly higher in PpSARKg and WT under salt stress conditions, suggesting that PpSARK might positively regulate salt tolerance via an ABA-related pathway. Endogenous ABA contents also increased 3-fold under salt stress conditions. These results indicate that PpSARK functions as a positive regulator in salt stress responses, while possibly functioning as a negative regulator in senescence in moss.

scholarly journals Expression Analysis of Multiple dnaK Genes in the Cyanobacterium Synechococcus elongatus PCC 7942

2007 ◽  
Vol 189 (10) ◽  
pp. 3751-3758 ◽  
Author(s):  
Masumi Sato ◽  
Kaori Nimura-Matsune ◽  
Satoru Watanabe ◽  
Taku Chibazakura ◽  
Hirofumi Yoshikawa
Keyword(s):  
Stress Response ◽  
Stress Responses ◽  
Light Stress ◽  
Synechococcus Elongatus ◽  
Stress Conditions ◽  
Heat Shock Gene ◽  
Upstream Region ◽  
Sequence Motif ◽  
Sequence Element ◽  
Pcc 7942

ABSTRACT We analyzed the stress responses of three dnaK homologues (dnaK1, dnaK2, and dnaK3) in the cyanobacterium Synechococcus elongatus PCC 7942. A reporter assay showed that under stress conditions the expression of only the dnaK2 gene was induced, suggesting a functional assignment of these homologues. RNA blot hybridization indicated a typical stress response of dnaK2 to heat and high-light stress. Primer extension mapping showed that dnaK2 was transcribed from similar sites under various stress conditions. Although no known sequence motif was detected in the upstream region, a 20-bp sequence element was highly conserved in dnaK2; it was essential not only for the stress induction but also for the basal expression of dnaK2. The ubiquitous upstream localization of this element in each heat shock gene suggests its important role in the cyanobacterial stress response.

scholarly journals Listeria monocytogenes σB Has a Small Core Regulon and a Conserved Role in Virulence but Makes Differential Contributions to Stress Tolerance across a Diverse Collection of Strains

2010 ◽  
Vol 76 (13) ◽  
pp. 4216-4232 ◽  
Author(s):  
H. F. Oliver ◽  
R. H. Orsi ◽  
M. Wiedmann ◽  
K. J. Boor
Keyword(s):  
Oxidative Stress ◽  
Listeria Monocytogenes ◽  
Stress Response ◽  
Cell Invasion ◽  
Stress Responses ◽  
Stress Conditions ◽  
Infection Model ◽  
Expression Microarrays ◽  
Phylogenetic Lineages ◽  
And Oxidative Stress

ABSTRACT Listeria monocytogenes strains are classified in at least three distinct phylogenetic lineages. There are correlations between lineage classification and source of bacterial isolation; e.g., human clinical and food isolates usually are classified in either lineage I or II. However, human clinical isolates are overrepresented in lineage I, while food isolates are overrepresented in lineage II. σB, a transcriptional regulator previously demonstrated to contribute to environmental stress responses and virulence in L. monocytogenes lineage II strains, was hypothesized to provide differential abilities for L. monocytogenes survival in various niches (e.g., food and human clinical niches). To determine if the contributions of σB to stress response and virulence differ across diverse L. monocytogenes strains, ΔsigB mutations were created in strains belonging to lineages I, II, IIIA, and IIIB. Paired parent and ΔsigB mutant strains were tested for survival under acid and oxidative stress conditions, Caco-2 cell invasion efficiency, and virulence using the guinea pig listeriosis infection model. Parent and ΔsigB mutant strain transcriptomes were compared using whole-genome expression microarrays. σB contributed to virulence in each strain. However, while σB contributed significantly to survival under acid and oxidative stress conditions and Caco-2 cell invasion in lineage I, II, and IIIB strains, the contributions of σB were not significant for these phenotypes in the lineage IIIA strain. A core set of 63 genes was positively regulated by σB in all four strains; different total numbers of genes were positively regulated by σB in the strains. Our results suggest that σB universally contributes to L. monocytogenes virulence but specific σB-regulated stress response phenotypes vary among strains.

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