scholarly journals Adhesion of active cytoskeletal vesicles

2018 ◽  
Author(s):  
R. Maan ◽  
E. Loiseau ◽  
A. R. Bausch
Keyword(s):  
Molecular Motors ◽  
Building Blocks ◽  
Membrane Area ◽  
Actin Cortex ◽  
Rigidity Sensing ◽  
Solid Substrates ◽  
Strong Adhesion ◽  
Glass Surfaces ◽  
Synthetic Biology Approach

AbstractRegulation of adhesion is a ubiquitous feature of living cells, observed during processes such as motility, antigen recognition or rigidity sensing. At the molecular scale, a myriad of mechanisms are necessary to recruit and activate the essential proteins, while at the cellular scale efficient regulation of adhesion relies on the cell’s ability to adapt its global shape. To understand the role of shape remodeling during adhesion, we use a synthetic biology approach to design a minimal model, starting with a limited number of building blocks. We assemble cytoskeletal vesicles whose size, reduced volume, and cytoskeleton contractility can be independently tuned. We are able to show that these cytoskeletal vesicles can sustain strong adhesion to solid substrates only if molecular motors are able to actively remodel the actin cortex. When the cytoskeletal vesicles are deformed under hypertonic osmotic pressure, they develop a crumpled geometry with huge deformations. In the presence of molecular motors, these deformations are dynamic in nature and can compensate for an absence of excess membrane area needed for adhesion to take place. When the cytoskeletal deformations are able to compensate for lack of excess membrane area, the cytoskeletal vesicles are able to attach to the rigid glass surfaces even under strong adhesive forces. The balance of deformability and adhesion strength is identified to be key to enable cytoskeletal vesicles to adhere to solid substrates.

The Role of the City in Merovingian Francia

2020 ◽  
pp. 581-610
Author(s):  
S. T. Loseby
Keyword(s):  
Roman Empire ◽  
Urban Communities ◽  
Late Antiquity ◽  
Building Blocks ◽  
Urban Network ◽  
City Councils ◽  
Royal Courts ◽  
Range Of Functions ◽  
Fierce Competition

The Merovingians inherited an urban network from the Roman Empire that remained substantially intact. Although Gallic cities had long been declining in extent and sophistication, during late antiquity their landscapes were adapted to contemporary priorities through the provision of walls and churches, and their politics was transformed by the emergence of bishops as leaders of urban communities. When the upper tiers of imperial administration disappeared, this equipped the vast majority of cities to survive as the basic building blocks of Merovingian kingdoms that were initially conceived as aggregations of city–territories. In ruling through their cities, the Merovingians expanded upon existing mechanisms for the extraction of taxes and services, while relying on centrally appointed bishops and counts rather than city councils for the projection of their authority. This generated fierce competition between kings for control of cities and among local elites for positions of power within them. In the later Merovingian period, however, the significance of cities diminished as stable territorial kingdoms emerged, political practice was centralized around the royal courts, and the Roman administrative legacy finally disintegrated. But the cities remained preeminent religious centers, and, with the beginnings of economic revival, continued to perform a range of functions unmatched by other categories of settlement.

scholarly journals Characterizing the building blocks of Problematic Use of the Internet (PUI): The role of obsessional impulses and impulsivity traits among Italian young adults

2021 ◽  
Vol 106 ◽  
pp. 152225
Author(s):  
Francesco Di Carlo ◽  
Mauro Pettorruso ◽  
Maria Chiara Alessi ◽  
Elena Picutti ◽  
Rebecca Collevecchio ◽  
...  
Keyword(s):  
Young Adults ◽  
Building Blocks ◽  
The Internet ◽  
Problematic Use ◽  
Use Of The Internet

scholarly journals Microtubule–microtubule sliding by kinesin-1 is essential for normal cytoplasmic streaming in Drosophila oocytes

2016 ◽  
Vol 113 (34) ◽  
pp. E4995-E5004 ◽  
Author(s):  
Wen Lu ◽  
Michael Winding ◽  
Margot Lakonishok ◽  
Jill Wildonger ◽  
Vladimir I. Gelfand
Keyword(s):  
Cytoplasmic Streaming ◽  
Cell Types ◽  
Nurse Cells ◽  
Wild Type ◽  
Actin Cortex ◽  
Microtubule Motor ◽  
Multiple Cell ◽  
Cytoplasmic Microtubules ◽  
Two Populations

Cytoplasmic streaming in Drosophila oocytes is a microtubule-based bulk cytoplasmic movement. Streaming efficiently circulates and localizes mRNAs and proteins deposited by the nurse cells across the oocyte. This movement is driven by kinesin-1, a major microtubule motor. Recently, we have shown that kinesin-1 heavy chain (KHC) can transport one microtubule on another microtubule, thus driving microtubule–microtubule sliding in multiple cell types. To study the role of microtubule sliding in oocyte cytoplasmic streaming, we used a Khc mutant that is deficient in microtubule sliding but able to transport a majority of cargoes. We demonstrated that streaming is reduced by genomic replacement of wild-type Khc with this sliding-deficient mutant. Streaming can be fully rescued by wild-type KHC and partially rescued by a chimeric motor that cannot move organelles but is active in microtubule sliding. Consistent with these data, we identified two populations of microtubules in fast-streaming oocytes: a network of stable microtubules anchored to the actin cortex and free cytoplasmic microtubules that moved in the ooplasm. We further demonstrated that the reduced streaming in sliding-deficient oocytes resulted in posterior determination defects. Together, we propose that kinesin-1 slides free cytoplasmic microtubules against cortically immobilized microtubules, generating forces that contribute to cytoplasmic streaming and are essential for the refinement of posterior determinants.

scholarly journals Resonance in Optimal Perturbation Evolution. Part II: Effects of a Nonzero Mean PV Gradient

2007 ◽  
Vol 64 (3) ◽  
pp. 695-710 ◽  
Author(s):  
H. de Vries ◽  
J. D. Opsteegh
Keyword(s):  
Potential Vorticity ◽  
Propagation Speed ◽  
Building Blocks ◽  
Joint Effect ◽  
Optimal Perturbation ◽  
Orr Mechanism ◽  
Nonzero Mean ◽  
Low Levels ◽  
Pv Gradient

Abstract Optimal perturbations are constructed for a two-layer β-plane extension of the Eady model. The surface and interior dynamics is interpreted using the concept of potential vorticity building blocks (PVBs), which are zonally wavelike, vertically confined sheets of quasigeostrophic potential vorticity. The results are compared with the Charney model and with the two-layer Eady model without β. The authors focus particularly on the role of the different growth mechanisms in the optimal perturbation evolution. The optimal perturbations are constructed allowing only one PVB, three PVBs, and finally a discrete equivalent of a continuum of PVBs to be present initially. On the f plane only the PVB at the surface and at the tropopause can be amplified. In the presence of β, however, PVBs influence each other’s growth and propagation at all levels. Compared to the two-layer f-plane model, the inclusion of β slightly reduces the surface growth and propagation speed of all optimal perturbations. Responsible for the reduction are the interior PVBs, which are excited by the initial PVB after initialization. Their joint effect is almost as strong as the effect from the excited tropopause PVB, which is also negative at the surface. If the optimal perturbation is composed of more than one PVB, the Orr mechanism dominates the initial amplification in the entire troposphere. At low levels, the interaction between the surface PVB and the interior tropospheric PVBs (in particular those near the critical level) takes over after about half a day, whereas the interaction between the tropopause PVB and the interior PVBs is responsible for the main amplification in the upper troposphere. In all cases in which more than one PVB is used, the growing normal mode configuration is not reached at optimization time.

Bouncing on thin air: how squeeze forces in the air film during non-wetting droplet bouncing lead to momentum transfer and dissipation

2015 ◽  
Vol 776 ◽  
pp. 531-567 ◽  
Author(s):  
Jolet de Ruiter ◽  
Rudy Lagraauw ◽  
Frieder Mugele ◽  
Dirk van den Ende
Keyword(s):  
Oscillation Mode ◽  
Interference Microscopy ◽  
Droplet Motion ◽  
Solid Substrates ◽  
Long Time ◽  
Lift Off ◽  
Continuous Presence ◽  
Air Film ◽  
Single Oscillation

Millimetre-sized droplets are able to bounce multiple times on flat solid substrates irrespective of their wettability, provided that a micrometre-thick air layer is sustained below the droplet, limiting $\mathit{We}$ to ${\lesssim}4$. We study the energy conversion during a bounce series by analysing the droplet motion and its shape (decomposed into eigenmodes). Internal modes are excited during the bounce, yet the viscous dissipation associated with the in-flight oscillations accounts for less than 20 % of the total energy loss. This suggests a significant contribution from the bouncing process itself, despite the continuous presence of a lubricating air film below the droplet. To study the role of this air film we visualize it using reflection interference microscopy. We quantify its thickness (typically a few micrometres) with sub-millisecond time resolution and ${\sim}30~\text{nm}$ height resolution. Our measurements reveal strong asymmetry in the air film shape between the spreading and receding phases of the bouncing process. This asymmetry is crucial for effective momentum reversal of the droplet: lubrication theory shows that the dissipative force is repulsive throughout each bounce, even near lift-off, which leads to a high restitution coefficient. After multiple bounces the droplet eventually hovers on the air film, while continuously experiencing a lift force to sustain its weight. Only after a long time does the droplet finally wet the substrate. The observed bounce mechanism can be described with a single oscillation mode model that successfully captures the asymmetry of the air film evolution.

scholarly journals The role of drop shape in impact and splash

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Qingzhe Liu ◽  
Jack Hau Yung Lo ◽  
Ye Li ◽  
Yuan Liu ◽  
Jinyu Zhao ◽  
...  
Keyword(s):  
Electromagnetic Field ◽  
Spherical Geometry ◽  
Impact Dynamics ◽  
Helmholtz Instability ◽  
Liquid Drops ◽  
Drop Shape ◽  
Spreading Dynamics ◽  
Solid Substrates ◽  
The Impact

AbstractThe impact and splash of liquid drops on solid substrates are ubiquitous in many important fields. However, previous studies have mainly focused on spherical drops while the non-spherical situations, such as raindrops, charged drops, oscillating drops, and drops affected by electromagnetic field, remain largely unexplored. Using ferrofluid, we realize various drop shapes and illustrate the fundamental role of shape in impact and splash. Experiments show that different drop shapes produce large variations in spreading dynamics, splash onset, and splash amount. However, underlying all these variations we discover universal mechanisms across various drop shapes: the impact dynamics is governed by the superellipse model, the splash onset is triggered by the Kelvin-Helmholtz instability, and the amount of splash is determined by the energy dissipation before liquid taking off. Our study generalizes the drop impact research beyond the spherical geometry, and reveals the potential of using drop shape to control impact and splash.

scholarly journals Myosin-II activity generates a dynamic steady state with continuous actin turnover in a minimal actin cortex

2018 ◽  
Author(s):  
Sonal ◽  
Kristina A. Ganzinger ◽  
Sven K. Vogel ◽  
Jonas Mücksch ◽  
Philipp Blumhardt ◽  
...  
Keyword(s):  
Steady State ◽  
Actin Polymerization ◽  
Myosin Ii ◽  
Fine Tuning ◽  
Cellular Functions ◽  
Actin Cortex ◽  
Actin Turnover ◽  
Cytoskeletal Dynamics

ABSTRACTDynamic reorganization of the actomyosin cytoskeleton allows a fine-tuning of cell shape that is vital to many cellular functions. It is well established that myosin-II motors generate the forces required for remodeling the cell surface by imparting contractility to actin networks. An additional, less understood, role of myosin-II in cytoskeletal dynamics is believed to be in the regulation of actin turnover; it has been proposed that myosin activity increases actin turnover in various cellular contexts, presumably by contributing to disassembly. In vitro reconstitution of actomyosin networks has confirmed the role of myosin in actin network disassembly, but factors such as diffusional constraints and the use of stabilized filaments have thus far limited the observation of myosin-assisted actin turnover in these networks. Here, we present the reconstitution of a minimal dynamic actin cortex where actin polymerization is catalyzed on the membrane in the presence of myosin-II activity. We demonstrate that myosin activity leads to disassembly and redistribution in this simplified cortex. Consequently, a new dynamic steady state emerges in which actin filaments undergo constant turnover. Our findings suggest a multi-faceted role of myosin-II in fast remodeling of the eukaryotic actin cortex.

scholarly journals Role of the Global Fund in national HIV/AIDS response in Myanmar: a qualitative study

2021 ◽  
Vol 6 (1) ◽  
Author(s):  
Zarni Htun ◽  
Yingxi Zhao ◽  
Hannah Gilbert ◽  
Chunling Lu
Keyword(s):  
Civil Society ◽  
Qualitative Study ◽  
Health System ◽  
Global Fund ◽  
Scale Up ◽  
Building Blocks ◽  
Key Informants ◽  
The Impact ◽  
Hiv Aids

Abstract Background The Global Fund has been a major funding source for HIV/AIDS programs in Myanmar. In this qualitative study, we aim to understand the impact of Global Fund on national HIV/AIDS response in Myanmar during the era of Millennium Development Goals (MDGs). Methods We conducted individual in-depth interviews by recruiting key informants through purposive snowball sampling. The respondents were engaged in the national/subnational response to HIV/AIDS in Myanmar and worked for the United Nations agencies, non-governmental organizations (NGOs), and civil society. Interview questions were organized around the role of Global Fund in strengthening national response to HIV/AIDS in the six building blocks of the Myanmar’s health system. Transcripts from the key informants were synthesized into specific themes through a deductive approach. Results We found that the Global Fund has provided substantial support to (1) finance the national HIV/AIDS response in Myanmar, and (2) strengthen leadership and governance at the central level through improving coordination and collaboration, including more stakeholders (e.g. civil society, NGOs) in decision making process, and catalyzing policy changes on scaling-up key interventions. Yet, its role remains limited in addressing new demands at the township level in terms of capacity building, staffing, and medical supply resulting from rapid scale-up of HIV interventions and decentralization of service delivery in the public sector. Conclusion There was a missed opportunity for Myanmar to capitalize on the use of the Global Fund’s funding to strengthen the health system. Deliberate planning is required to optimize the use of those scarce resources to provide universal coverage for HIV/AIDS.

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