scholarly journals Scalable cytoarchitectonic characterization of large intact human neocortex samples

2018 ◽  
Author(s):  
Sven Hildebrand ◽  
Anna Schueth ◽  
Andreas Herrler ◽  
Ralf Galuske ◽  
Alard Roebroeck
Keyword(s):  
Refractive Index ◽  
Low Cost ◽  
Adult Brain ◽  
Human Cortex ◽  
Optical Clearing ◽  
Volume Imaging ◽  
Index Matching ◽  
Refractive Index Matching ◽  
Formalin Fixed

AbstractWe describe MASH (Multiscale Architectonic Staining of Human cortex): a simple, fast and low-cost cytoarchitectonic labeling and optical clearing approach for human cortex samples, which can be applied to large formalin fixed adult brain samples. A suite of small-molecule fluorescent nuclear and cytoplasmic dyes in combination with new refractive index matching solutions allows deep volume imaging. This enables highly scalable human neocortical cytoarchitecture characterization with a large 3D scope.

scholarly journals A tunable refractive index matching medium for live imaging cells, tissues and model organisms

eLife ◽  
2017 ◽  
Vol 6 ◽  
Author(s):  
Tobias Boothe ◽  
Lennart Hilbert ◽  
Michael Heide ◽  
Lea Berninger ◽  
Wieland B Huttner ◽  
...  
Keyword(s):  
Refractive Index ◽  
Image Quality ◽  
Light Microscopy ◽  
Penetration Depth ◽  
Cell Cultures ◽  
Model Organisms ◽  
Optical Clearing ◽  
Index Matching ◽  
Refractive Index Matching ◽  
Tunable Refractive Index

In light microscopy, refractive index mismatches between media and sample cause spherical aberrations that often limit penetration depth and resolution. Optical clearing techniques can alleviate these mismatches, but they are so far limited to fixed samples. We present Iodixanol as a non-toxic medium supplement that allows refractive index matching in live specimens and thus substantially improves image quality in live-imaged primary cell cultures, planarians, zebrafish and human cerebral organoids.

scholarly journals Refractive index matching applied to fecal smear clearing

2005 ◽  
Vol 47 (6) ◽  
pp. 347-350 ◽  
Author(s):  
Cláudio S. Ferreira
Keyword(s):  
Refractive Index ◽  
Sucrose Solution ◽  
Glycerol Solution ◽  
Human Feces ◽  
Microscope Slide ◽  
Helminth Eggs ◽  
Index Matching ◽  
Refractive Index Matching ◽  
Fecal Smear ◽  
Effect Of Light

Thick smears of human feces can be made adequate for identification of helminth eggs by means of refractive index matching. Although this effect can be obtained by simply spreading a fleck of feces on a microscope slide, a glycerol solution has been routinely used to this end. Aiming at practicability, a new quantitative technique has been developed. To enhance both sharpness and contrast of the images, a sucrose solution (refractive index = 1.49) is used, which reduces the effect of light-scattering particulates. To each slide a template-measured (38.5 mm³) fecal sample is transferred. Thus, egg counts and sensitivity evaluations are easily made.

scholarly journals Biological glass: structural determinants of eye lens transparency

2011 ◽  
Vol 366 (1568) ◽  
pp. 1250-1264 ◽  
Author(s):  
Steven Bassnett ◽  
Yanrong Shi ◽  
Gijs F. J. M. Vrensen
Keyword(s):  
Refractive Index ◽  
Cellular Level ◽  
Structural Determinants ◽  
Lens Transparency ◽  
Index Matching ◽  
Lens Fibre ◽  
Refractive Index Matching ◽  
Lens Membranes ◽  
Adhesive Proteins ◽  
Structural Adaptations

The purpose of the lens is to project a sharply focused, undistorted image of the visual surround onto the neural retina. The first pre-requisite, therefore, is that the tissue should be transparent. Despite the presence of remarkably high levels of protein, the lens cytosol remains transparent as a result of short-range-order interactions between the proteins. At a cellular level, the programmed elimination of nuclei and other light-scattering organelles from cells located within the pupillary space contributes directly to tissue transparency. Scattering at the cell borders is minimized by the close apposition of lens fibre cells facilitated by a plethora of adhesive proteins, some expressed only in the lens. Similarly, refractive index matching between lens membranes and cytosol is believed to minimize scatter. Refractive index matching between the cytoplasm of adjacent cells is achieved through the formation of cellular fusions that allow the intermingling of proteins. Together, these structural adaptations serve to minimize light scatter and enable this living, cellular structure to function as ‘biological glass’.

scholarly journals Kilowatt-level direct-‘refractive index matching liquid’-cooled Nd:YLF thin disk laser resonator

2016 ◽  
Vol 24 (2) ◽  
pp. 1758 ◽  
Author(s):  
Zhibin Ye ◽  
Chong Liu ◽  
Bo Tu ◽  
Ke Wang ◽  
Qingsong Gao ◽  
...  
Keyword(s):  
Refractive Index ◽  
Thin Disk ◽  
Laser Resonator ◽  
Disk Laser ◽  
Index Matching ◽  
Refractive Index Matching ◽  
Thin Disk Laser
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