scholarly journals Cross-Platform Mechanical Characterization of Lung Tissue

2018 ◽  
Author(s):  
Samuel R. Polio ◽  
Aritra N. Kundu ◽  
Carey E. Dougan ◽  
Nathan P. Birch ◽  
D. Ezra Aurian-Blajeni ◽  
...  
Keyword(s):  
Lung Tissue ◽  
Tissue Mechanics ◽  
Published Data ◽  
Small Amplitude Oscillatory Shear ◽  
Uniaxial Testing ◽  
Order Of Magnitude ◽  
Cross Platform ◽  
Cavitation Rheology

ABSTRACTPublished data on the mechanical strength and elasticity of lung tissue is widely variable, primarily due to differences in how testing was conducted across individual studies. This makes it extremely difficult to find a benchmark modulus of lung tissue when designing synthetic extracellular matrices (ECMs). To address this issue, we tested tissues from various areas of the lung using multiple characterization techniques, including micro-indentation, small amplitude oscillatory shear (SAOS), uniaxial tension, and cavitation rheology. We report the sample preparation required and data obtainable across these unique but complimentary methods to quantify the modulus of lung tissue. We highlight cavitation rheology as a new method, which can measure the modulus of intact tissue with precise spatial control, and reports a modulus on the length scale of typical tissue heterogeneities. Shear rheology, uniaxial, and indentation testing require heavy sample manipulation and destruction; however, cavitation rheology can be performed in situ across nearly all areas of the lung with minimal preparation. The Young’s modulus of bulk lung tissue using microindentation (1.9±0.5 kPa), SAOS (3.2±0.6 kPa), uniaxial testing (3.4±0.4 kPa), and cavitation rheology (6.1±1.6 kPa) were within the same order of magnitude, with higher values consistently reported from cavitation, likely due to our ability to keep the tissue intact. Although cavitation rheology does not capture the non-linear strains revealed by uniaxial testing and SAOS, it provides an opportunity to measure mechanical characteristics of lung tissue on a microscale level on intact tissues. Overall, our study demonstrates that each technique has independent benefits, and each technique revealed unique mechanical features of lung tissue that can contribute to a deeper understanding of lung tissue mechanics.

Permeability characterization of the Soultz and Ogachi large‐scale reservoir using induced microseismicity

Geophysics ◽  
2002 ◽  
Vol 67 (1) ◽  
pp. 204-211 ◽  
Author(s):  
Pascal Audigane ◽  
Jean‐Jacques Royer ◽  
Hideshi Kaieda
Keyword(s):  
Large Scale ◽  
Pressure Oscillation ◽  
Data Sets ◽  
Active Volume ◽  
Common Procedure ◽  
Order Of Magnitude ◽  
Different Depths ◽  
The Difference

Hydraulic fracturing is a common procedure to increase the permeability of a reservoir. It consists in injecting high‐pressure fluid into pilot boreholes. These hydraulic tests induce locally seismic emission (microseismicity) from which large‐scale permeability estimates can be derived assuming a diffusion‐like process of the pore pressure into the surrounding stimulated rocks. Such a procedure is applied on six data sets collected in the vicinity of two geothermal sites at Soultz (France) and Ogachi (Japan). The results show that the method is adequate to estimate large‐scale permeability tensors at different depths in the reservoir. Such an approach provides permeability of the medium before fracturing compatible with in situ measurements. Using a line source formulation of the diffusion equation rather than a classical point source approach, improvements are proposed for accounting in situation where the injection is performed on a well section. This technique applied to successive fluid‐injection tests indicates an increase in permeability by an order of magnitude. The underestimates observed in some cases are attributed to the difference of scale at which the permeability is estimated (some 1 km3 corresponding to the seismic active volume of rock compared to a few meters around the well for the pumping or pressure oscillation tests). One advantage of the proposed method is that it provides permeability tensor estimates at the reservoir scale.

scholarly journals Colonial choanoflagellate isolated from Mono Lake harbors a microbiome

2021 ◽  
Author(s):  
Kayley Hake ◽  
Patrick T West ◽  
Kent L. McDonald ◽  
Davis Laundon ◽  
Crystal Feng ◽  
...  
Keyword(s):  
Mono Lake ◽  
Close Relative ◽  
Purple Sulfur Bacteria ◽  
Isolation And Characterization ◽  
Sulfur Bacteria ◽  
Hybridization Probes ◽  
Order Of Magnitude ◽  
Large Spherical

Choanoflagellates offer key insights into bacterial influences on the origin and early evolution of animals. Here we report the isolation and characterization of a new colonial choanoflagellate species, Salpingoeca monosierra, that, unlike previously characterized species, harbors a stable microbiome. S. monosierra was isolated from Mono Lake, California and forms large spherical colonies that are more than an order of magnitude larger than those formed by the closely related S. rosetta. By designing fluorescence in situ hybridization probes from metagenomic sequences, we found that S. monosierra colonies are colonized by members of the halotolerant and closely related Saccharospirillaceae and Oceanospirillaceae, as well as purple sulfur bacteria (Ectothiorhodospiraceae) and non-sulfur Rhodobacteraceae. This relatively simple microbiome in a close relative of animals presents a new experimental model for investigating the evolution of stable interactions among eukaryotes and bacteria.

scholarly journals Electrochemical preparation and in situ characterization of poly(3-methylpyrrole) and poly(3-methylpyrrole-cyclodextrin) films on gold electrodes

2008 ◽  
Vol 6 (2) ◽  
pp. 199-207 ◽  
Author(s):  
Jalal Arjomandi ◽  
Rudolf Holze
Keyword(s):  
Lithium Perchlorate ◽  
Oxidation Peak ◽  
Potential Cycling ◽  
In Situ Characterization ◽  
Electrochemical Preparation ◽  
Vis Spectroscopy ◽  
Order Of Magnitude ◽  
Minimum Resistance

AbstractA Electrosynthesis of conducting poly(3-methylpyrrole) (P3MPy) and poly(3-methylpyrrole-2,6-dimethyl-β-cyclodextrin) (poly(3MPy-β-DMCD)) films on a gold electrode in acetonitrile electrolyte solution containing lithium perchlorate has been carried out by potential cycling. Products were characterized with cyclic voltammetry CV, in situ UV-Vis spectroscopy, and in situ resistance measurements. Electrosynthesis of poly(3MPy-β-DMCD) started with a (1:1) (3MPy-β-DMCD) supramolecular cyclodextrin CD complex of 3-methylpyrrole characterized with proton NMR spectroscopy. The oxidation peak of poly(3MPy-β-DMCD) in CVs is shifted to more positive values than P3MPy. In situ resistance measurements show that the resistance of poly(3MPy-β-DMCD) is higher than of P3MPy by approximately an order of magnitude. Minimum resistance can be observed for P3MPy and poly(3MPy-β-DMCD) at 0.40 E Ag/AgClE Ag/AgClIn situ UV-Vis spectroelectrochemical data for both films prepared potentiodynamically by cycling the potential in the range −0.20 E Ag/AgCl

scholarly journals Mitigation of airways responsiveness by deep inflation of the lung

2018 ◽  
Vol 124 (6) ◽  
pp. 1447-1455 ◽  
Author(s):  
Jason H. T. Bates ◽  
Vignesh Rajendran
Keyword(s):  
Airway Responsiveness ◽  
Actin Binding ◽  
Critical Threshold ◽  
Published Data ◽  
Temporary Reduction ◽  
Threshold Strain ◽  
Order Of Magnitude ◽  
Cross Bridges ◽  
Dose Dependent

Stretching activated strips of airway smooth muscle (ASM) significantly affects both active force and stiffness due to a temporary reduction of the proportion of cycling myosin cross bridges that are bound to their actin binding sites. For the same reason, stretch applied to ASM in situ by a deep inflation (DI) of the lungs is one of the most potent means of reversing bronchoconstriction. When the DI is sufficiently large, however, and is applied while bronchoconstriction is in the process of developing, the subsequent depression in airway resistance is more persistent than can be attributed simply to temporary detachment of ASM cross bridges. In the present study, we use a computational model to demonstrate that this DI-induced ablation of airway responsiveness can be explained by a dose-dependent reduction in the number of cross bridges available to bind to actin when the ASM in the airway wall is stretched above a critical threshold strain and that this disruption of the contractile apparatus recovers over an order of magnitude longer time scale than that of the simple reattachment of unbound cross bridges. NEW & NOTEWORTHY The mechanisms by which deep inflation of the lung reverse bronchoconstriction and affect subsequent airway responsiveness have important potential implications for asthma, yet remain controversial. This study uses computational modeling to posit a mechanism by which sufficiently vigorous inflations applied during active bronchoconstriction not only transiently reverse bronchoconstriction, but also reduce subsequent airways responsiveness for a period of time. Fitting the model to published data in mice supports this notion.

scholarly journals Cross-platform mechanical characterization of lung tissue

PLoS ONE ◽  
2018 ◽  
Vol 13 (10) ◽  
pp. e0204765 ◽  
Author(s):  
Samuel R. Polio ◽  
Aritra Nath Kundu ◽  
Carey E. Dougan ◽  
Nathan P. Birch ◽  
D. Ezra Aurian-Blajeni ◽  
...  
Keyword(s):  
Lung Tissue ◽  
Mechanical Characterization ◽  
Cross Platform

The Use of Advanced Analytical Techniques for Characterization of the Chemical, Physical, and Crystallographic Nature of a Surface

1973 ◽  
Vol 31 ◽  
pp. 132-133 ◽  
Author(s):  
R. E. Herfert
Keyword(s):  
Surface Characterization ◽  
Analytical Techniques ◽  
X Ray Diffraction ◽  
Depth Of Penetration ◽  
X Ray ◽  
The Past ◽  
Transmission Electron ◽  
Scanning Electron

Studies of the nature of a surface, either metallic or nonmetallic, in the past, have been limited to the instrumentation available for these measurements. In the past, optical microscopy, replica transmission electron microscopy, electron or X-ray diffraction and optical or X-ray spectroscopy have provided the means of surface characterization. Actually, some of these techniques are not purely surface; the depth of penetration may be a few thousands of an inch. Within the last five years, instrumentation has been made available which now makes it practical for use to study the outer few 100A of layers and characterize it completely from a chemical, physical, and crystallographic standpoint. The scanning electron microscope (SEM) provides a means of viewing the surface of a material in situ to magnifications as high as 250,000X.

In situ microprobe quantitation of inorganic particle burden in paraffin sections of lung tissue

1988 ◽  
Vol 46 ◽  
pp. 990-991
Author(s):  
Jerrold L. Abraham
Keyword(s):  
Lung Tissue ◽  
Quantitative Information ◽  
Particulate Material ◽  
Paraffin Sections ◽  
Tissue Samples ◽  
Qualitative And Quantitative ◽  
The Past ◽  
Quantitative Analyses ◽  
Data Result

Inorganic particulate material of diverse types is present in the ambient and occupational environment, and exposure to such materials is a well recognized cause of some lung disease. To investigate the interaction of inhaled inorganic particulates with the lung it is necessary to obtain quantitative information on the particulate burden of lung tissue in a wide variety of situations. The vast majority of diagnostic and experimental tissue samples (biopsies and autopsies) are fixed with formaldehyde solutions, dehydrated with organic solvents and embedded in paraffin wax. Over the past 16 years, I have attempted to obtain maximal analytical use of such tissue with minimal preparative steps. Unique diagnostic and research data result from both qualitative and quantitative analyses of sections. Most of the data has been related to inhaled inorganic particulates in lungs, but the basic methods are applicable to any tissues. The preparations are primarily designed for SEM use, but they are stable for storage and transport to other laboratories and several other instruments (e.g., for SIMS techniques).

A very rapid in situ Kikuchi technique to determine relative crystal misorientation

1988 ◽  
Vol 46 ◽  
pp. 830-831
Author(s):  
J. I. Bennetch
Keyword(s):  
Electron Beam ◽  
Analytical Techniques ◽  
Superplastic Forming ◽  
The Other ◽  
Kikuchi Pattern ◽  
Kikuchi Line ◽  
Line Analysis

In a recent study of the superplastic forming (SPF) behavior of certain Al-Li-X alloys, the relative misorientation between adjacent (sub)grains proved to be an important parameter. It is well established that the most accurate way to determine misorientation across boundaries is by Kikuchi line analysis. However, the SPF study required the characterization of a large number of (sub)grains in each sample to be statistically meaningful, a very time-consuming task even for comparatively rapid Kikuchi analytical techniques.In order to circumvent this problem, an alternate, even more rapid in-situ Kikuchi technique was devised, eliminating the need for the developing of negatives and any subsequent measurements on photographic plates. All that is required is a double tilt low backlash goniometer capable of tilting ± 45° in one axis and ± 30° in the other axis. The procedure is as follows. While viewing the microscope screen, one merely tilts the specimen until a standard recognizable reference Kikuchi pattern is centered, making sure, at the same time, that the focused electron beam remains on the (sub)grain in question.

Coherent electron nanodiffraction from clean silver nano particles in a UHV STEM

1993 ◽  
Vol 51 ◽  
pp. 1058-1059
Author(s):  
J. Liu ◽  
M. Pan ◽  
G. E. Spinnler
Keyword(s):  
Supported Catalysts ◽  
Imaging Techniques ◽  
Nano Particles ◽  
Metal Particles ◽  
Shape Structure ◽  
Dynamical Simulations ◽  
Small Metal Particles ◽  
Extract Information

Small metal particles have peculiar chemical and physical properties as compared to bulk materials. They are especially important in catalysis since metal particles are common constituents of supported catalysts. The structural characterization of small particles is of primary importance for the understanding of structure-catalytic activity relationships. The shape and size of metal particles larger than approximately 5 nm in diameter can be determined by several imaging techniques. It is difficult, however, to deduce the shape of smaller metal particles. Coherent electron nanodiffraction (CEND) patterns from nano particles contain information about the particle size, shape, structure and defects etc. As part of an on-going program of STEM characterization of supported catalysts we report some preliminary results of CEND study of Ag nano particles, deposited in situ in a UHV STEM instrument, and compare the experimental results with full dynamical simulations in order to extract information about the shape of Ag nano particles.

Characterization of the glycoconjugates of boar testis and epididymis

Reproduction ◽  
2000 ◽  
pp. 325-335 ◽  
Author(s):  
A Calvo ◽  
LM Pastor ◽  
S Bonet ◽  
E Pinart ◽  
M Ventura
Keyword(s):  
Ricinus Communis ◽  
Lectin Histochemistry ◽  
Apical Part ◽  
Seminiferous Tubules ◽  
In Situ Characterization ◽  
Intense Staining ◽  
Terminal Galactose ◽  
Boar Spermatozoa

Lectin histochemistry was used to perform in situ characterization of the glycoconjugates present in boar testis and epididymis. Thirteen horseradish peroxidase- or digoxigenin-labelled lectins were used in samples obtained from healthy fertile boars. The acrosomes of the spermatids were stained intensely by lectins with affinity for galactose and N-acetyl-galactosamine residues, these being soybean, peanut and Ricinus communis agglutinins. Sertoli cells were stained selectively by Maackia ammurensis agglutinin. The lamina propria of seminiferous tubules showed the most intense staining with fucose-binding lectins. The Golgi area and the apical part of the principal cells of the epididymis were stained intensely with many lectins and their distribution was similar in the three zones of the epididymis. On the basis of lectin affinity, both testis and epididymis appear to have N- and O-linked glycoconjugates. Spermatozoa from different epididymal regions showed different expression of terminal galactose and N-acetyl-galactosamine. Sialic acid (specifically alpha2,3 neuraminic-5 acid) was probably incorporated into spermatozoa along the extratesticular ducts. These findings indicate that the development and maturation of boar spermatozoa are accompanied by changes in glycoconjugates. As some lectins stain cellular or extracellular compartments specifically, these lectins could be useful markers in histopathological evaluation of diseases of boar testis and epididymis.

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