scholarly journals Endoglycosidase S enables a highly simplified clinical chemistry assay procedure for direct assessment of serum IgG undergalactosylation in chronic inflammatory disease

2018 ◽  
Author(s):  
Dieter Vanderschaeghe ◽  
Leander Meuris ◽  
Tom Raes ◽  
Hendrik Grootaert ◽  
Annelies Van Hecke ◽  
...  
Keyword(s):  
Analytical Procedure ◽  
Clinical Chemistry ◽  
Wide Spectrum ◽  
Chronic Inflammatory Disease ◽  
Clinical Implementation ◽  
Antibody Purification ◽  
The Past ◽  
Serum Igg ◽  
Assay Procedure ◽  
Igg Purification

AbstractOver the past 30 years, it has been firmly established that a wide spectrum of (autoimmune) diseases such as rheumatoid arthritis, Crohn’s and lupus, but also other pathologies like alcoholic and non-alcoholic steatohepatitis (ASH and NASH) are driven by chronic inflammation and are hallmarked by a reduced level of serum IgG galactosylation. IgG (under)galactosylation is a promising biomarker to assess disease severity, and monitor and adjust therapy. However, this biomarker has not been implemented in routine clinical chemistry due to a complex analytical procedure that necessitates IgG purification, which is difficult to perform and validate at high throughput.We addressed this issue by using endo-β-N-acetylglucosaminidase from Streptococcus pyogenes (endoS) to specifically release Fc N-glycans in whole serum. The entire assay can be completed in a few hours and only entails adding endoS and labeling the glycans with APTS. Glycans are then readily analyzed through capillary electrophoresis. We demonstrate in two independent patient cohorts that IgG undergalactosylation levels obtained with this assay correlate very well with scores calculated from PNGaseF-released glycans of purified antibodies. Our new assay allows to directly and specifically measure the degree of IgG galactosylation in serum through a fast and completely liquid phase protocol, without the requirement for antibody purification. This should help advancing this biomarker towards clinical implementation.

scholarly journals Endoglycosidase S Enables a Highly Simplified Clinical Chemistry Procedure for Direct Assessment of Serum IgG Undergalactosylation in Chronic Inflammatory Disease

2018 ◽  
Vol 17 (12) ◽  
pp. 2508-2517 ◽  
Author(s):  
Dieter Vanderschaeghe ◽  
Leander Meuris ◽  
Tom Raes ◽  
Hendrik Grootaert ◽  
Annelies Van Hecke ◽  
...  
Keyword(s):  
Inflammatory Disease ◽  
Clinical Chemistry ◽  
Chronic Inflammatory Disease ◽  
Direct Assessment ◽  
Serum Igg

Impact of microRNAs on molecular epidemiology

2011 ◽  
Vol 152 (16) ◽  
pp. 633-641 ◽  
Author(s):  
Katalin Gőcze ◽  
Katalin Gombos ◽  
Gábor Pajkos ◽  
Ingrid Magda ◽  
Ágoston Ember ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Cancer Research ◽  
Molecular Epidemiology ◽  
Wide Spectrum ◽  
Translation Regulation ◽  
Rna Sequences ◽  
Serum Mirna ◽  
The Past ◽  
Non Coding Rna ◽  
Free Serum

Cancer research concerning short non-coding RNA sequences and functionally linked to RNA interference (RNAi) have reached explosive breakthrough in the past decade. Molecular technology applies microRNA in extremely wide spectrum from molecular tumor prediction, diagnostics, progression monitoring and prevention. Functional analysis of tissue miRNA and cell-free serum miRNA in posttranscription and translation regulation innovated and restructured the knowledge on the field. This review focuses on molecular epidemiology and primary prevention aspects of the small non-coding RNA sequences. Orv. Hetil., 2011, 152, 633–641.

Recent Advances in Therapeutic Applications of Bisbenzimidazoles

2020 ◽  
Vol 16 (4) ◽  
pp. 454-486 ◽  
Author(s):  
Smita Verma ◽  
Vishnuvardh Ravichandiran ◽  
Nihar Ranjan ◽  
Swaran J.S. Flora
Keyword(s):  
Heterocyclic Compounds ◽  
Wide Spectrum ◽  
Dna Recognition ◽  
Present Review ◽  
Pharmacological Activities ◽  
Structural Motifs ◽  
Therapeutic Applications ◽  
The Past ◽  
Recent Advances

Nitrogen-containing heterocycles are one of the most common structural motifs in approximately 80% of the marketed drugs. Of these, benzimidazoles analogues are known to elicit a wide spectrum of pharmaceutical activities such as anticancer, antibacterial, antiparasitic, antiviral, antifungal as well as chemosensor effect. Based on the benzimidazole core fused heterocyclic compounds, crescent-shaped bisbenzimidazoles were developed which provided an early breakthrough in the sequence-specific DNA recognition. Over the years, a number of functional variations in the bisbenzimidazole core have led to the emergence of their unique properties and established them as versatile ligands against several classes of pathogens. The present review provides an overview of diverse pharmacological activities of the bisbenzimidazole analogues in the past decade with a brief account of its development through the years.

Cucurbitacins as Anticancer Agents: A Patent Review

2019 ◽  
Vol 14 (2) ◽  
pp. 133-143 ◽  
Author(s):  
Hidayat Hussain ◽  
Ivan R. Green ◽  
Muhammad Saleem ◽  
Khanzadi F. Khattak ◽  
Muhammad Irshad ◽  
...  
Keyword(s):  
Anticancer Agents ◽  
Wide Spectrum ◽  
Biological Effects ◽  
Therapeutic Effects ◽  
Cytotoxic Effects ◽  
Natural Sources ◽  
Oh Groups ◽  
Drug Candidates ◽  
The Past ◽  
Wide Range

Background: Cucurbitacins belong to a group of tetracyclic triterpenoids that display a wide range of biological effects. In the past, numerous cucurbitacins have been isolated from natural sources and many active compounds have been synthesized using the privileged scaffold in order to enhance its cytotoxic effects. Objective: his review covers patents on the therapeutic effects of natural cucurbitacins and their synthetic analogs published during the past decade. By far, the majority of patents published are related to cancer and Structure-Activity Relationships (SAR) of these compounds are included to lend gravitas to this important class of natural products. Methods: The date about the published patents was downloaded via online open access patent databases. Results: Cucurbitacins display significant cytotoxic properties, in particular cucurbitacins B and D which possess very potent effects towards a number of cancer cells. Numerous cucurbitacins isolated from natural sources have been derivatized through chemical modification at the C(2)-OH and C(25)- OH groups. Most importantly, an acyl ester of the C(25)-OH and, iso-propyl, n-propyl and ethyl ether groups of the C(2)-OH demonstrated the most increased cytotoxic activity. Conclusion: The significant cytotoxic effects of natural and semi-synthetic cucurbitacins make them attractive as new drug candidates. Moreover, cucurbitacins have the capability to form conjugates with other anticancer drugs which will synergistically enhance their anticancer effects. The authors believe that in order to get lead compounds, there should be a greater focus on the synthesis of homodimers, heterodimers, and halo derivatives of cucurbitacins. In the opinion of the authors the analysis of the published patents on the cucurbitacins indicates that these compounds can be developed into a regimen to treat a wide spectrum of cancers.

Toward Unity among Environmentalists

1995 ◽  
Author(s):  
Bryan G. Norton
Keyword(s):  
Resource Use ◽  
Wide Spectrum ◽  
Strong Support ◽  
Environmental Issues ◽  
Value Systems ◽  
Special Interests ◽  
Unified Approach ◽  
Policy Makers ◽  
The Past ◽  
Control Growth

Today, six out of ten Americans describe themselves as "active" environmentalists or as "sympathetic" to the movement's concerns. The movement, in turn, reflects this millions-strong support in its diversity, encompassing a wide spectrum of causes, groups, and sometimes conflicting special interests. For far-sighted activists and policy makers, the question is how this diversity affects the ability to achieve key goals in the battle against pollution, erosion, and out-of-control growth. This insightful book offers an overview of the movement -- its past as well as its present -- and issues the most persuasive call yet for a unified approach to solving environmental problems. Focusing on examples from resource use, pollution control, protection of species and habitats, and land use, the author shows how the dynamics of diversity have actually hindered environmentalists in the past, but also how a convergence of these interests around forward-looking policies can be effected, despite variance in value systems espoused. The book is thus not only an assessment of today's movement, but a blueprint for action that can help pull together many different concerns under a common banner. Anyone interested in environmental issues and active approaches to their solution will find the author's observations both astute and creative.

Twisted oxide lateral homostructures with conjunction tunability

2021 ◽  
Author(s):  
Jan-Chi Yang ◽  
Ping-Chun Wu ◽  
Chia-Chun Wei ◽  
Qilan Zhong ◽  
Sheng-Zhu Ho ◽  
...  
Keyword(s):  
Wide Spectrum ◽  
Strain Engineering ◽  
Epitaxial Films ◽  
Modern Technology ◽  
Specific Substrate ◽  
Lattice Structures ◽  
High Quality ◽  
The Past ◽  
Sharp Interfaces ◽  
Selection Of

Abstract Epitaxial growth is of significant importance over the past decades, given it has been the key process of modern technology for delivering high-quality thin films. For conventional heteroepitaxy, the selection of proper single crystal substrates not only facilitates the integration of different materials but also fulfills interface and strain engineering upon a wide spectrum of functionalities. Nevertheless, the lattice structures, regularity and crystalline orientation are determined once a specific substrate is chosen. In this work, we reveal the growth of twisted oxide lateral homostructures with multiple conjunction degree of freedom. The twisted lateral homostructures with atomically sharp interfaces can be composed of epitaxial “blocks” with different crystalline orientations, ferroic orders and phases. We further demonstrate that this approach is universal for fabricating various complex systems. Our results establish an efficient pathway towards twisted lateral homostructures, allowing epitaxial films to be arbitrarily tailored at designated positions with unbounded in-plane conjunction tunability.

Automated clinical chemistry and haematology in a hospital region

1973 ◽  
Vol 184 (1077) ◽  
pp. 361-368
Keyword(s):  
Northern Ireland ◽  
Clinical Chemistry ◽  
Progressive Development ◽  
Analytical Instruments ◽  
The Past ◽  
Routine Work ◽  
Large Teaching Hospital ◽  
Clinical Chemical ◽  
The Impact ◽  
Flow Of Information

The impact of increasing analytical sophistication has, over the past 20 years, resulted in a remorseless increase in the number of requests submitted to hospital laboratories each year. Increasing numbers of requests led in both the clinical chemical and haematological laboratories to a search for mechanized or automated techniques which would enable a limited number of staff to achieve increases in productivity. In this way, over the past 15 years, there has been a progressive development of analytical instruments of greater and greater versatility whose advent has, to a very large extent, transformed the work of the clinical chemist and the laboratory haematologist and has often, by its very capacity for work, confronted them with a surfeit of data. The necessity to process this increasing flow of information has, in many cases, led to the use of dedicated laboratory computers and to some extent it can be said to have stimulated the concept of centralization at least of the broad mass of routine work in the clinical chemistry and haematology laboratories. This paper describes the steps taken in the laboratories of a large teaching hospital in Northern Ireland to move to a position where such centralization is not only possible but logical.

scholarly journals Implementation: The Next Giant Hurdle to Clinical Transformation With Digital Health

10.2196/16259 ◽  
2019 ◽  
Vol 21 (11) ◽  
pp. e16259 ◽  
Author(s):  
Lorraine Buis
Keyword(s):  
Health Research ◽  
Digital Health ◽  
Internet Research ◽  
Clinical Implementation ◽  
The Past ◽  
Barriers To Implementation ◽  
Clinical Transformation

Clinical implementation of digital health is a major hurdle to overcome in the coming years. Considering the role of the Journal of Medical Internet Research in the past 20 years and looking toward the journal’s future, this viewpoint acknowledges the vision of medicine and the role that digital health plays in that vision. It also highlights barriers to implementation of digital health as an obstacle to achieving that vision. In particular, this paper focuses on how digital health research must start looking toward implementation as an area of inquiry and the role that the Journal of Medical Internet Research and its' sister journals from JMIR Publications can play in this process.

An update report on the harmonization of adult reference intervals in Australasia

2018 ◽  
Vol 0 (0) ◽  
Author(s):  
Gus Koerbin ◽  
Ken Sikaris ◽  
Graham R.D. Jones ◽  
Robert Flatman ◽  
Jillian R. Tate
Keyword(s):  
Royal College ◽  
Clinical Chemistry ◽  
Metrological Traceability ◽  
Reference Intervals ◽  
Evidence Based ◽  
Blood Gas ◽  
Arterial Blood Gas ◽  
The Past ◽  
Arterial Blood ◽  
Blood Gas Parameters

Abstract The Australasian Association of Clinical Biochemists (AACB) has over the past 5 years been actively working to achieve harmonized reference intervals (RIs) for common clinical chemistry analytes using an evidence-based checklist approach where there is sound calibration and metrological traceability. It has now recommended harmonized RIs for 18 common clinical chemistry analytes which are performed in most routine laboratories and these have been endorsed by the Royal College of Pathologists of Australasia (RCPA). In 2017 another group of analytes including urea, albumin and arterial blood gas parameters were considered and suggested harmonized RIs proposed. This report provides an update of those harmonization efforts.

The current state of the pharmaceutical market for anthelmintic drugs for veterinary use

2021 ◽  
Vol 1 (9) ◽  
pp. 37-44
Author(s):  
A. K. Klyueva ◽  
◽  
A. A. Deltsov ◽  
K. O. Belova ◽  
◽  
...  
Keyword(s):  
Russian Federation ◽  
Wide Spectrum ◽  
Pharmaceutical Market ◽  
The Past ◽  
Veterinary Medicines ◽  
Current State ◽  
Prevention And Treatment ◽  
The Russian Federation ◽  
State Registration ◽  
Anthelmintic Drugs

This paper presents an analysis of the range of veterinary medicines registered in the territory of the Russian Federation and used for the prevention and treatment of helminthiasis. The Russian Federation is a leader in the development and production of anthelmintic medicines registered on its territory. Antiparasitic medicines used for the prevention and treatment of helminthiasis are divided according to the spectrum of action depending on the class of helminths. It was revealed that the majority of medicines (89 medicines – 58,17%) have a wide spectrum of action. Of these, 62 medicines (40,52% of the total amount of anthelmintic medicines) are intended for the treatment of nematodes and cestodoses. When distributing anthelmintic medicines among target animals, it was found that 75 medicines (49.02%) are intended for the treatment of productive animals, and 78 medicines (50,98%) are intended for the treatment of unproductive animals. When assessing the dynamics of state registration of anthelmintic medicines for veterinary use, it can be noted that over the past 4 years there has been a tendency towards an increase in the average annual registration.

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