scholarly journals Microbial Treatment of Soil Contaminated with Spent engine Oil / Biotreatment of Soil Contaminated with Spent Engine by Microorganisms

2018 ◽  
Author(s):  
A. A. Ayandele
Keyword(s):  
Organic Matter ◽  
Contaminated Soil ◽  
Heterotrophic Bacteria ◽  
Ph Value ◽  
Soil Samples ◽  
Engine Oil ◽  
Contaminated Site ◽  
Degrading Bacteria ◽  
Spent Engine Oil ◽  
Test Organisms

AbstractThe potential of six microorganisms (Pseudomonas aeruginosa, Micrococcus sp, Flavobacterium sp, Rhizopus sp, Penicillium sp and Fusarium sp) isolated from hydrocarbon contaminated site were evaluated for their biodegradation ability. The soil samples were contaminated with 5% (w/v) of spent engine oil and the rate of biodegradation of the oil was studied for a period of 10weeks under greenhouse experiment. The total heterotrophic bacteria count (THBC), total hydrocarbon degrading bacteria count (THDBC), physicochemical and heavy metals properties of the soil samples and Total Petroleum Hydrocarbon (TPH) were determined after treatment with test organisms. THBC and THDBC ranged from 0.175 to 0.280 CFUg-1 and 0.47 CFUg-1 respectively for the control plot, while THBC is ranging from 0.197 to 0.275 CFUg-1 and THDBC was 0.180 to 0. 473 CFUg-1 for the contaminated plot. There was a slight increase in the pH value of the contaminated soil sample and the treated soil samples as the experimental weeks increased. The results obtained showed a significant decrease (at p ≤ 0.05) in the nutrients content of the soil samples. There was an increase from 1.09 in the control to 15.5% in the content of organic matter after contamination and from 1.88% to 26.8% in the % of organic matter too. There was a significant reduction (at p ≤ 0.05) in the concentration of Fe, Zn, Pb, Cd, Cu, Cr and Ni after 10 weeks of incubation with the tested organisms. Plant growth in the treated contaminated soil samples ranged from 32.6cm to 38.6cm, while that of the control 1 (Uncontaminated soil) was 51.2cm and 19.7cm high was observed in the Control 2 (contaminated untreated soil) after 22 days of the experiment. The TPH degradation (% loss) ranged from 79.7 to 89. 2% after 10 weeks of treatment. P. aeruginosa had the highest level of degradation (89.2%), while Micrococcus sp and Rhizopus sp had the least degradation at 79.9%.All the microorganisms used in this study had the abilities to remediating soil contaminated with spent engine oil and the remediated soil samples were able to support the growth of Zea mays at 5% (w/v) level of contamination.

Gravimetric Profile of Hydrocarbon Degrading Bacterial and Fungal Isolates from Contaminated Soil Samples in Ado-Ekiti, Nigeria

2021 ◽  
Vol 1 (1) ◽  
Author(s):  
Olayinka O. Idris ◽  
◽  
Olayinka T. Ogunmefun ◽  
Cinderella N. Tuesimi
Keyword(s):  
Crude Oil ◽  
Contaminated Soil ◽  
Contaminated Soils ◽  
Soil Samples ◽  
Engine Oil ◽  
Water Retention Capacity ◽  
Trichoderma Spp ◽  
Fungal Isolates ◽  
Degrading Bacteria ◽  
Spent Engine Oil

One of the biological compounds limiting soil water retention capacity is oil when present due to its hydro-nature. However, some microorganisms exhibit the capacity to degrade oil as a source of carbon, whereby the soil quality is retained and enhanced. Hence, the gravimetric profile of hydrocarbon degrading bacteria and fungi isolated from oil contaminated soil samples was investigated. Soil samples were collected from surface and 10m depth from six different mechanic workshops and generator sites. The pour plate technique was used to isolate the microorganisms. All pure isolates were sub-cultured using Bushnell Haas agar and the isolated bacteria were identified by their morphological and biochemical characteristics. The soil samples pH range was 4.3 - 6.4. Bacteria isolated included Pseudomonas spp., Staphylococcus spp., Microccocus spp., Acinetobacter spp., and Bacillus spp. The fungi isolated included Aspergillus spp., Rhizopus spp., Candida spp., Trichoderma spp. and Penicillium spp. Degradation of kerosene, diesel, crude oil, engine oil, and spent engine oil was allowed using Acinetobacter baumanni, P. aeruginosa, B. subtilis, and S. aureus. Gravimetric analyses were used to determine the percentage of petroleum hydrocarbon degraded by bacterial isolates. The highest percentage of degradation was between P. aeruginosa and B. subtilis. Pseudomonas aeruginosa degraded 97.4% diesel, 88.2% kerosene, 71.3% crude oil, 80.7% engine oil and 78.2% spent engine oil; while Bacillus subtilis degraded 71% diesel, 97% kerosene, 89.6% crude oil, 87% engine oil and 72.6% spent engine oil. This study revealed that bacterial and fungal isolates from oil contaminated soils exhibited the potentials to degrade oil and bioremediation using these microorganisms was possible.

scholarly journals Phenylobacterium Korensee Best Indigenous Petroleum Hydrocarbon Degrading Bacteria Isolated from Contaminated Soil of Bahror, Alwar Region, India

2019 ◽  
Vol 10 (08) ◽  
pp. 20203-20211 ◽  
Author(s):  
Mohan Lal Kuri ◽  
Vidhya Kumari ◽  
Shikha Roy
Keyword(s):  
Contaminated Soil ◽  
Engine Oil ◽  
Contaminated Site ◽  
Spectroscopy Analysis ◽  
Global Issue ◽  
Degrading Bacteria ◽  
Term Basis ◽  
Agar Media ◽  
Gas Chromatography Mass Spectroscopy

Contamination of soil, water and air due to hydrocarbons are a global issue and bioremediation provides probably the best way to remediate the contaminants. The current study shows the biodegradation of crude oil, diesel and used engine oil by a newly isolated Phenylobacterium korensee from contaminated soil of Bahror, Alwar, Rajasthan. Hydrocarbon degrading strain was screened on BHA (Bushnell Haas Agar) media supplemented with 2T engine oil as sole carbon source. The strain was found to be degrading at 1%, 4% and 10% of used 2T engine oil respectively after 14 days. Degradation was confirmed both gravimetrically and by Gas Chromatography Mass Spectroscopy analysis. The degradation was found very well at long term basis. The optimization of growth also studied at temperature and pH basis also. The significance of the study is that the percentage degradation of the complex petroleum supplements used in the study was found to be far higher than some of the previously reported values and this bacterial strain was firstly found from this contaminated site.

scholarly journals Bioremediation of Spent Engine Oil on Selected Contaminated Soils within Ilorin Metropolis

2020 ◽  
Vol 8 (1) ◽  
pp. 91-104
Author(s):  
Elizabeth Adeyinka AJIBOYE ◽  
Hikmat Omolara SULAYMAN ◽  
Abdullahi Taiwo AJAO
Keyword(s):  
Heavy Metals ◽  
Organic Matter ◽  
Organic Carbon ◽  
Contaminated Soils ◽  
Physicochemical Parameters ◽  
Soil Samples ◽  
Engine Oil ◽  
Biochemical Tests ◽  
Spent Engine Oil ◽  
Before And After

The research aimed to investigate the bioremediation of spent engine oil on selected contaminated soils within Ilorin metropolis. To achieve this, soil samples were collected from three (3) mechanic workshops along Taiwo axis within the metropolis. The soil samples were then subjected to bioremediation using the land-farming approach. The physicochemical parameters of the soil samples before and after bioremediation were analyzed using standard methods. Bacteria were isolated using standard procedures and identified using biochemical tests and molecular methods. Results for the physicochemical parameters of the soil samples before bioremediation include particle size (all sandy in nature); pH (6.00 ± 0.14 - 6.20 ± 0.14); Organic carbon (14.65 ± 3.20 - 17.54 ± 1.87), Organic matter (33.50 ± 0.85 - 43.45 ± 9.12) and heavy metals (ND - 11.74 ± 0.07). Values after bioremediation for pH, organic carbon, organic matter and heavy metals were 8.25 ± 0.07 - 8.90 ± 0.14, 13.07 ± 0.05 - 13.25 ± 0.84, 37.25 ± 1.06 - 44.80 ± 1.13, ND - 9.40 ± 0.04 respectively. Values for bacterial count before and after bioremediation of the soil samples were 8.00  1.41 - 67.50 ± 2.12 x 105 CFU/mL and 6.50 ± 2.12 - 164.00 ± 11.31 x 105 CFU/mL respectively. Bacterial isolates were identified as Pseudomonas sp., Enterobacter sp., Acinetobacter sp., and Bacillus sp. while the hydrocarbon-utilizing bacteria were identified as Thalassospira mesophila strain JCM 18969; Pseudomonas fluorescens F113; Siccibacter turicensis LMG 23730; Pseudomonas Zeshuii strain KACC 15471; Pseudomonas stutzeri strain CGMCC 1.1803 and Marinobacter hydrocarbonoclasticus strain ATCC 49840. In conclusion, the bacteria isolates effectively bioremediated the spent engine oil contaminated soils with a reduction of hydrocarbon pollutants.

scholarly journals Biodegradation of spent engine oil by bacteria isolated from the rhizosphere of legumes grown in contaminated soil

2014 ◽  
Vol 3 (2) ◽  
pp. 63-75 ◽  
Author(s):  
HY Ismail ◽  
UJJ Ijah ◽  
ML Riskuwa ◽  
II Allamin
Keyword(s):  
Molecular Weight ◽  
Contaminated Soil ◽  
Rhizosphere Soil ◽  
Enrichment Culture ◽  
Bacterial Species ◽  
Engine Oil ◽  
Klebsiella Pneumonia ◽  
Lablab Purpureus ◽  
Degrading Bacteria ◽  
Spent Engine Oil

Biodegradation of spent engine oil (SEO) by bacteria isolated from the rhizosphere of Cajan cajan and Lablab purpureus was investigated. It was with a view to determining most efficient bacterial species that could degrade SEO in phytoremediation studies. Hydrocarbon degrading bacteria were isolated and identified by enrichment culture technique using oil agar supplemented with 0.1% v/v SEO. Total heterotrophic and oil utilizing bacterial count showed the occurrence of large number of bacteria predominantly in the rhizosphere soil, ranging between 54×108 - 144×108 CFU/g and 4×108- 96×108 CFU/g respectively. Percentage of oil utilizing bacteria ranged between 0% (uncontaminated non rhizosphere soil) to 76% (contaminated rhizosphere). Turbidimetrically, five bacterial species namely Pseudomonas putrefacience CR33, Klebsiella pneumonia CR23, Pseudomonas alcaligenes LR14, Klebsiella aerogenes CR21, and Bacillus coagulans CR31 were shown to grow maximally and degraded the oil at the rate of 68%, 62%, 59%, 58%and 45% respectively. Chromatographic analysis using GC-MS showed the presence of lower molecular weight hydrocarbons in the residual oil (indicating degradation) after 21 days, whereas the undegraded oil (control) had higher molecular weight hydrocarbons after the same period. The species isolated were shown to have high ability of SEO biodegradation and therefore could be important tools in ameliorating SEO contaminated soil. DOI: http://dx.doi.org/10.3126/ije.v3i2.10515 International Journal of the Environment Vol.3(2) 2014: 63-75

scholarly journals Bioremediation of Soil Contaminated with Spent Engine Oil using Pig Dung

2021 ◽  
pp. 68-78
Author(s):  
A. A. Ibiene ◽  
O. Aleruchi ◽  
L.C. Nnodim ◽  
R. U. Ihunwo
Keyword(s):  
Contaminated Soil ◽  
Physicochemical Parameters ◽  
Petroleum Hydrocarbon ◽  
Soil Samples ◽  
Total Petroleum Hydrocarbon ◽  
Engine Oil ◽  
Microbiological Analysis ◽  
Ph Values ◽  
Port Harcourt ◽  
Spent Engine Oil

Studies were carried out to investigate the bioremediation potential of pig dung in a soil contaminated with spent engine oil. Soil samples were obtained from the Ofrima complex, University of Port Harcourt. The soil samples were contaminated with various concentrations (50 ml and 100 ml) of spent engine oil and allowed for 21 days for proper exposure, mimicking natural spill. This was followed by the addition of the pig dung. The experimental setup was labeled sample A (1 kg soil + 100 g pig dung + 50 ml spent engine oil) and sample B (1 kg soil + 100 g pig dung + 100 ml spent engine oil). The physicochemical parameters and the microbiological analysis were done using standard methods. The total petroleum hydrocarbon was analyzed using gas chromatographic methods. Analyses were carried out at 14 days intervals for 28 days. The physicochemical parameter results showed a reduction in pH values in the contaminated soil samples, ranging from 6.21 - 6.65 in sample A and 6.57 - 6.87 in sample B. Temperature values were constant at 230C from day 1 to day 14 in sample A and increased at day 28 to 24 0C, also for sample B, the temperature was constant at 230C from day 1 to day14 and increased at day 28 to 26 0C. The amount of heavy metal (Lead) content decreased from 4.3645 - 1.93676 (mg/kg) and 6.18361 - 3.89654 (mg/kg) for samples A and B, respectively. There was also a significant reduction in the amount of Total Petroleum Hydrocarbon, from 16631.86 - 3280.83 mg/kg for sample A and 18464.73 - 6784.60 mg/kg for sample B. The THB counts for samples A and B ranged from 7.73 - 7.91 and 7.05-8.20 (Log cfu/g), respectively. The fungal counts ranged from 3.99–4.58 and 5.12 - 7.93 (Log cfu/g) for samples A and B respectively. HUB counts ranged from 4.52–5.09 and 4.93- 5.55 (Log cfu/g) for samples A and B, respectively. The HUF counts ranged from 4.12 - 5.49 and 4.13 - 4.70 (Log cfu/g) for samples A and B, respectively. The results clearly showed that microorganisms capable of utilizing total petroleum hydrocarbon were present, also the pig dung showed both bio-stimulation and bio-augmentation tendency to attract high microbial load which supported the bioremediation of the spent engine oil contaminated soil.

scholarly journals Biological Treatment of Used Engine Oil by Single and Mixed Bacterial Cultures Isolated from Soil of Mechanic Workshops

2018 ◽  
Vol 12 (1) ◽  
pp. 115-123
Author(s):  
Amel Hussein ◽  
Saad Khudhair
Keyword(s):  
Soil Samples ◽  
Growth Patterns ◽  
Engine Oil ◽  
Gravimetric Analysis ◽  
Bacterial Isolates ◽  
Engine Oils ◽  
Used Engine Oil ◽  
Degrading Bacteria ◽  
Spent Engine Oil ◽  
Consortium Culture

The accumulation of hydrocarbon waste, such as used engine oils in environments, has many impacts on humans and other organisms, therefore many researches were achieved to degrade or remove or consume these pollutants. The aim of the current study is to get a local bacterial isolates has high ability to degrade the spent engine oil as a single or mixed culture. Five soil samples contaminated with spent engine oil were collected from mechanic workshops in Baghdad city to isolate degrading bacteria using Bushnell Hans medium (BHM), pH 7 with 5% of used engine oil. While the growth patterns and gravimetric analysis was used to reveal the ability of these isolates to degrade spent engine oil in liquid BHM medium. The best three isolates A4, B6 and D5 were identified and the optimal temperature and pH for biodegradation of spending engine oil were studied. Also, the consortium culture of three isolates was tested their ability to utilize spent engine oil under the same conditions for single isolate. Twenty five bacterial isolates were obtained from contaminated soil samples and three isolates appeared a maximum degradation rate 74.6, 70.2 and 78.5% respectively. The results from identification tests were showing these isolates belong to Bacillus sp., Acinetobacter sp. and Pseudomonas sp., respectively. The studied three isolates gave the best degradation when incubated at 30°C in BHM medium pH 7. While other results were indicated that consortium cultures are more effective 90.2% than all experiments that used single isolate.

scholarly journals Biodegradation of used Engine Oil Contaminated Site using Individual and Mixed bacterial isolates

2020 ◽  
pp. 38-45
Author(s):  
Muh'd A. J. ◽  
Sa'adatu A.Y. ◽  
Surayya M. M. ◽  
Sa'adatu M. I. ◽  
Nafisa B. ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacillus Cereus ◽  
Contaminated Soil ◽  
Bacterial Species ◽  
Soil Samples ◽  
Engine Oil ◽  
Contaminated Site ◽  
Bacterial Isolates ◽  
The World ◽  
Degradation Ability

Oil is most widely distributed source of energy in the world and large-scaled environmental pollutant. Oil, oil products, and oil containing industrial waste pollution is ranked second place after radioactive pollution on account of their harmful action to ecosystems. Contamination of soil by organic chemicals (mostly hydrocarbons) is prevalent in oil producing and industrialized countries of the world. Biodegradation, a strategy that uses biological means (i.e microbes) to degrade, stabilize and remove soil contaminants is an alternative green technology remediation of hydrocarbon contaminated soil. This study was carried out to isolate and screen Bacteria capable of degrading used engine oil from oil contaminated site. Soil samples were collected from oil contaminated site. Bacterial species were isolated from the collected soil samples by serial dilution and agar methods. Different bacterial species were isolated but only four were oil degrading isolates. The identity of the various genera of bacterial contaminants were determined by a combination of cultural, morphological as well as preliminary biochemical characteristics of the isolates. The four oil degrading bacteria (AJ1, AJ2, AJ3 and AJ4) were preliminarily identified as Bacillus cereus, Pseudomonas aeruginosa, Bacillus subtillis and Micrococcus spp respectively. The degradation ability of the bacterial isolates was screened and maximum degradation was recorded by AJ 5 (Mixed culture) with 66.9 %, followed by Bacillus cereus (50.3 %), Bacillus subtilis (44 %), Pseudomonas aeruginosa (37.9 %) and the least was seen in Micrococcus spp (35.3 %). These findings revealed that some bacteria species are capable of utilizing the oil and used it as sole source of carbon and energy and the mixed consortia of the bacteria have rapid degradation ability. Biological degradation of hydrocarbon contaminated soil offers a better and more environmentally friendly technique that if properly explored can bring our environment into a better place for both plant and animal.

scholarly journals Describing Phosphorus Sorption Processes on Volcanic Soil in the Presence of Copper or Silver Engineered Nanoparticles

Minerals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 373
Author(s):  
Jonathan Suazo-Hernández ◽  
Erwin Klumpp ◽  
Nicolás Arancibia-Miranda ◽  
Patricia Poblete-Grant ◽  
Alejandra Jara ◽  
...  
Keyword(s):  
Organic Matter ◽  
Crop Production ◽  
Agricultural Soils ◽  
Ph Value ◽  
Consumer Products ◽  
Soil Samples ◽  
Engineered Nanoparticles ◽  
P Availability ◽  
Agricultural Crop ◽  
Phosphorus Sorption

Engineered nanoparticles (ENPs) present in consumer products are being released into the agricultural systems. There is little information about the direct effect of ENPs on phosphorus (P) availability, which is an essential nutrient for crop growthnaturally occurring in agricultural soils. The present study examined the effect of 1, 3, and 5% doses of Cu0 or Ag0 ENPs stabilized with L-ascorbic acid (suspension pH 2–3) on P ad- and desorption in an agricultural Andisol with total organic matter (T-OM) and with partial removal of organic matter (R-OM) by performing batch experiments. Our results showed that the adsorption kinetics data of H2PO4− on T-OM and R-OM soil samples with and without ENPs were adequately described by the pseudo-second-order (PSO) and Elovich models. The adsorption isotherm data of H2PO4− from T-OM and R-OM soil samples following ENPs addition were better fitted by the Langmuir model than the Freundlich model. When the Cu0 or Ag0 ENPs doses were increased, the pH value decreased and H2PO4− adsorption increased on T-OM and R-OM. The H2PO4− desorption (%) was lower with Cu0 ENPs than Ag0 ENPs. Overall, the incorporation of ENPs into Andisols generated an increase in P retention, which may affect agricultural crop production.

scholarly journals Phytoremediation of Spent Oil and Palm Kernel Sludge Contaminated Soil Using Sunflower (Helianthus annuus) L

2021 ◽  
Vol 25 (5) ◽  
pp. 877-885
Author(s):  
A.J. Odebode ◽  
K.L. Njoku ◽  
A.A. Adesuyi ◽  
M.O. Akinola
Keyword(s):  
Helianthus Annuus ◽  
Plant Height ◽  
Palm Oil ◽  
Contaminated Soil ◽  
Palm Kernel ◽  
Engine Oil ◽  
Palm Kernel Oil ◽  
Kernel Oil ◽  
Spent Engine Oil ◽  
And Control

This study was carried out to investigate the phytotoxicity of spent engine oil and palm kernel sludge on seed germination, seedling early growth and survival of sunflower (Helianthus annuus L) and its phytoremediating potential. 8.0 kg topsoil mixed with 2, 4, 6, 8 and 10% (w/v) of spent engine oil and palm kernel sludge, while the control was not mixed with spent oil and sludge (0%). The seeds were sown on these soils and monitored daily. Parameters taken were; plant height, leaf number and stem girth. The result showed that spent engine oil treated plants adversely affected growth compared to palm kernel sludge plants and control which performed better. For plant height, the mean stem girth for control at 2nd week was 0.40±0.05 mm, spent engine oil was 5.96±0.97 palm kernel oil effluent was 14.73±1.16 and at 12th week, control was 1.30±0.05 while for SEO the plant had withered and 124.6±9.02 for POE. Number of leaves at the 12th week was 26.00±2.08 in the control, 8.66±0.66, for spent engine oil at 4%, while for palm oil effluent it was 27.66±0.66, at 4%, concentration respectively. Stem girth at 2 weeks for spent engine oil was 0.19±0.05 at 2%, 0.43±0.03 for palm kernel oil effluent and at the 12th week of planting at 10% concentration was 1.63±0.08 for palm kernel oil effluent, and all plants had withered off for spent engine oil at same concentration at the 12th week. Also, spent engine oil at all concentrations delayed the germination of Helianthus annuus by 2days compared to control. Comparison analysis test showed that growth in untreated plants were significantly higher (p>0.05) than spent oil and palm kernel sludge treated plants. Similar result was observed for leaf number and stem girth which had higher mean value in palm kernel sludge and control compared to spent oil. Sunflower grown in 8% and 10% palm kernel sludge contaminated soil also flowered eight days earlier than control plants, while spent oil treated plant did not. The result shows that sunflower cannot tolerate high (4%, 6%, 8% and 10%) concentrations of spent engine oil in soil compared to palm oil effluent. Therefore, spent engine oil should be properly disposed because of its adverse effect on the growth and yield of sunflower.

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