scholarly journals Application of the Mesolens for sub-cellular resolution imaging of intact larval and whole adult Drosophila

2018 ◽  
Author(s):  
Gail McConnell ◽  
William B. Amos
Keyword(s):  
Salivary Glands ◽  
Reproductive System ◽  
Mouse Embryos ◽  
Intact Female ◽  
Entire Volume ◽  
Cellular Resolution ◽  
Resolution Imaging ◽  
Imaginal Disks ◽  
Adult Drosophila

AbstractIn a previous paper (McConnell et al., 2016) we showed a new giant lens called the Mesolens and presented performance data and images from whole fixed and intact fluorescently-stained 12.5-day old mouse embryos. Here we show that using the Mesolens we can image an entire Drosophila larva or adult fly in confocal epifluorescence and show sub-cellular detail in all tissues. By taking several hundreds of optical sections through the entire volume of the specimen, we show cells and nuclear details within the gut, brain, salivary glands and reproductive system that normally require dissection for study. Organs are imaged in situ in correct 3D arrangement. Imaginal disks are imaged in mature larvae and it proved possible to image pachytene chromosomes in cells within ovarian follicles in intact female flies. Methods for fixing, staining and clearing are given.

Super-Resolution Imaging in Fluid Mechanics Using New Illumination Approaches

2020 ◽  
Vol 52 (1) ◽  
pp. 369-393
Author(s):  
Minami Yoda
Keyword(s):  
Fluid Mechanics ◽  
Total Internal Reflection ◽  
Imaging Techniques ◽  
Super Resolution ◽  
Internal Reflection ◽  
Interfacial Flows ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Entire Volume ◽  
Resolution Imaging

Quantifying submillimeter flows using optical diagnostic techniques is often limited by a lack of spatial resolution and optical access. This review discusses two super-resolution imaging techniques, structured illumination microscopy and total internal reflection fluorescence or microscopy, which can visualize bulk and interfacial flows, respectively, at spatial resolutions below the classic diffraction limits. First, we discuss the theory and applications of structured illumination for optical sectioning, i.e., imaging a thin slice of a flow illuminated over its entire volume. Structured illumination can be used to visualize the interior of multiphase flows such as sprays by greatly reducing secondary scattering. Second, the theory underlying evanescent waves is introduced, followed by a review of how total internal reflection microscopy has been used to visualize interfacial flows over the last 15 years. Both techniques, which are starting to be used in fluid mechanics, could significantly improve quantitative imaging of microscale and macroscale flows.

scholarly journals Author response: Brain-wide cellular resolution imaging of Cre transgenic zebrafish lines for functional circuit-mapping

2019 ◽  
Author(s):  
Kathryn M Tabor ◽  
Gregory D Marquart ◽  
Christopher Hurt ◽  
Trevor S Smith ◽  
Alexandra K Geoca ◽  
...  
Keyword(s):  
Author Response ◽  
Transgenic Zebrafish ◽  
Cellular Resolution ◽  
Resolution Imaging

Expression of beta-1 integrin in human developing salivary glands and its parallel relation with maturation markers: In situ hybridisation and immunofluorescence study

2007 ◽  
Vol 52 (11) ◽  
pp. 1064-1071 ◽  
Author(s):  
Silvia Vanessa Lourenço ◽  
Dirce Mary C. Lima ◽  
Sabrina Hitomi Uyekita ◽  
Regina Schultz ◽  
Thales de Brito
Keyword(s):  
Salivary Glands ◽  
In Situ Hybridisation ◽  
Immunofluorescence Study ◽  
Beta 1 Integrin

scholarly journals High-resolution imaging of normal anatomy, and neural and adrenal malformations in mouse embryos using magnetic resonance microscopy

2003 ◽  
Vol 202 (2) ◽  
pp. 239-247 ◽  
Author(s):  
Jurgen E. Schneider ◽  
Simon D. Bamforth ◽  
Cassandra R. Farthing ◽  
Kieran Clarke ◽  
Stefan Neubauer ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
High Resolution ◽  
Mouse Embryos ◽  
Magnetic Resonance Microscopy ◽  
High Resolution Imaging ◽  
Normal Anatomy ◽  
Resolution Imaging

Design and Applications of Environmental Cell Transmission Electron Microscope for In Situ Observations of Gas–Solid Reactions

2001 ◽  
Vol 7 (6) ◽  
pp. 494-506 ◽  
Author(s):  
Renu Sharma
Keyword(s):  
Chemical Vapor ◽  
Atomic Level ◽  
Chemical Information ◽  
Oxidation Reduction ◽  
Transmission Electron ◽  
Recent Developments ◽  
Level Information ◽  
Resolution Imaging ◽  
Electron Energy Loss

AbstractThe environmental transmission electron microscopy (E-TEM) is a budding technique for in situ study of gas–solid chemical reactions with numerous applications. Recent improvements in the design have made it possible not only to obtain atomic level information but also the chemical information during the reaction by incorporating an imaging filter or electron energy-loss spectrometer to an E-TEM. We have been involved in modifying a couple of microscopes to incorporate environmental cells in order to convert them into E-TEMs. These microscopes have been used to obtain atomic level information of the structural and chemical changes during dynamic processes by in situ electron diffraction, high-resolution imaging, and electron energyloss spectroscopy. The applications include, but are not limited to, oxidation, reduction, polymerization, nitridation, dehydroxylation, hydroxylation, chemical vapor deposition, etc. We report recent developments in the design and application along with the limitations of an E-TEM.

Developmental expression of c-kit, a proto-oncogene encoded by the W locus

Development ◽  
1990 ◽  
Vol 109 (4) ◽  
pp. 911-923 ◽  
Author(s):  
A. Orr-Urtreger ◽  
A. Avivi ◽  
Y. Zimmer ◽  
D. Givol ◽  
Y. Yarden ◽  
...  
Keyword(s):  
Germ Cells ◽  
Receptor Tyrosine Kinase ◽  
Germ Line ◽  
Primordial Germ Cells ◽  
Mouse Embryos ◽  
Developmental Expression ◽  
Male Germ Line ◽  
Polypeptide Growth Factors ◽  
Adult Ovary

Developmental expression of the c-kit proto-oncogene, a receptor tyrosine kinase encoded by the W locus, was investigated by in situ hybridization in normal mouse embryos. Early after implantation transcripts were detectable only in the maternal placenta (6 1/2-7 1/2 days p.c.). Subsequently (8 1/2 days p.c.) numerous ectodermal (neural tube, sensory placodes) and endodermal (embryonic gut) derivatives expressed c-kit. Later transcripts were detected also in the blood islands of the yolk sac and in the embryonic liver, the main sites of embryonic hemopoiesis. Around midgestation, transcripts accumulated in the branchial pouches and also in primordial germ cells of the genital ridges. This complex pattern of expression remained characteristic also later in gestation, when c-kit was expressed in highly differentiated structures of the craniofacial area, in presumptive melanoblasts and in the CNS. In the adult ovary, maternal c-kit transcripts were detected. They were present in the oocytes of both immature and mature ovarian follicles, but not in the male germ line, where c-kit expression may be down regulated. Thus, c-kit activity is complex and appears in multiple tissues including those that also display defects in mutations at the W locus where c-kit is encoded. Correlation between W phenotypes and c-kit expression, as well as the regulation of the complex and multiple expression of polypeptide growth factors and receptors, is discussed.

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