scholarly journals Heat shock induces the depletion of Oct4 in mouse blastocysts and stem cells

2018 ◽  
Author(s):  
Mo-bin Cheng ◽  
Xue Wang ◽  
Yue Huang ◽  
Ye Zhang
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Heat Shock ◽  
Embryonic Development ◽  
Embryonic Stem ◽  
Mammalian Embryo ◽  
Death Associated Protein Kinase ◽  
Oct4 Protein ◽  
Ubiquitin Proteasome ◽  
Proteasome Pathway

AbstractTemperature is an important microenvironmental factor that functions epigenetically in normal embryonic development. However, the effect of heat shock in the stem cells is not fully understood. Oct4 is a tightly regulated master regulator of pluripotency maintenance in stem cells and during early embryonic development. We report here that Oct4 protein level was significantly reduced under heat shock in mouse blastocysts and embryonic stem cells. The reduction in Oct4 in the mouse embryonic stem cells under heat shock was mediated by a ubiquitin-proteasome pathway that was dependent on the activity of death- associated protein kinase 1 (Dapk1) to phosphorylate its substrate, Pin1. Our results imply that the depletion of Oct4 via brief heat shock, such as a high fever, during early pregnancy might severely impair the growth of the mammalian embryo or even cause its death.

Germ line transmission of an inactive N-myc allele generated by homologous recombination in mouse embryonic stem cells

1990 ◽  
Vol 10 (12) ◽  
pp. 6755-6758
Author(s):  
B R Stanton ◽  
S W Reid ◽  
L F Parada
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Homologous Recombination ◽  
Embryonic Development ◽  
Cell Lines ◽  
Germ Line ◽  
Es Cells ◽  
Embryonic Stem ◽  
Es Cell ◽  
Germ Line Transmission

We have disrupted one allele of the N-myc locus in mouse embryonic stem (ES) cells by using homologous recombination techniques and have obtained germ line transmission of null N-myc ES cell lines with transmission of the null N-myc allele to the offspring. The creation of mice with a deficient N-myc allele will allow the generation of offspring bearing null N-myc alleles in both chromosomes and permit study of the role that this proto-oncogene plays in embryonic development.

scholarly journals BAF Complex in Embryonic Stem Cells and Early Embryonic Development

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Heyao Zhang ◽  
Xuepeng Wang ◽  
Jingsheng Li ◽  
Ronghua Shi ◽  
Ying Ye
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Development ◽  
Embryonic Stem ◽  
Early Embryonic Development ◽  
Mouse Escs ◽  
Baf Complex ◽  
Pluripotency Gene ◽  
Chromatin Remodeling Complexes ◽  
Gene Regulatory

Embryonic stem cells (ESCs) can self-renew indefinitely and maintain their pluripotency status. The pluripotency gene regulatory network is critical in controlling these properties and particularly chromatin remodeling complexes. In this review, we summarize the research progresses of the functional and mechanistic studies of BAF complex in mouse ESCs and early embryonic development. A discussion of the mechanistic bases underlying the distinct phenotypes upon the deletion of different BAF subunits in ESCs and embryos will be highlighted.

scholarly journals Insights into the ubiquitin-proteasome system of human embryonic stem cells

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Isabel Saez ◽  
Seda Koyuncu ◽  
Ricardo Gutierrez-Garcia ◽  
Christoph Dieterich ◽  
David Vilchez
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Ubiquitin Proteasome System ◽  
Ubiquitin Proteasome

Differential effects of high and low strength magnetic fields on mouse embryonic development and vasculogenesis of embryonic stem cells

2016 ◽  
Vol 65 ◽  
pp. 46-58 ◽  
Author(s):  
Mohamed M. Bekhite ◽  
Andreas Finkensieper ◽  
Fouad A. Abou-Zaid ◽  
Ibrahim K. El-Shourbagy ◽  
Nabil K. EL-Fiky ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Magnetic Fields ◽  
Embryonic Development ◽  
Embryonic Stem ◽  
Differential Effects ◽  
Low Strength

scholarly journals Heat shock induces apoptosis in human embryonic stem cells but a premature senescence phenotype in their differentiated progeny

Cell Cycle ◽  
2012 ◽  
Vol 11 (17) ◽  
pp. 3260-3269 ◽  
Author(s):  
Larisa L. Alekseenko ◽  
Victoria I. Zemelko ◽  
Valery V. Zenin ◽  
Nataly A. Pugovkina ◽  
Irina V. Kozhukharova ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Heat Shock ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Premature Senescence ◽  
Senescence Phenotype

scholarly journals BRPF3-HUWE1-mediated regulation of MYST2 is required for differentiation and cell-cycle progression in embryonic stem cells

2020 ◽  
Vol 27 (12) ◽  
pp. 3273-3288
Author(s):  
Hye In Cho ◽  
Min Seong Kim ◽  
Jina Lee ◽  
Byong Chul Yoo ◽  
Kyung Hee Kim ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Development ◽  
Cell Cycle Progression ◽  
Histone Acetyltransferase ◽  
Embryoid Body ◽  
Embryonic Stem ◽  
Negative Regulator ◽  
Cycle Progression

AbstractBrpf-histone acetyltransferase (HAT) complexes have important roles in embryonic development and regulating differentiation in ESCs. Among Brpf family, Brpf3 is a scaffold protein of Myst2 histone acetyltransferase complex that plays crucial roles in gene regulation, DNA replication, development as well as maintaining pluripotency in embryonic stem cells (ESCs). However, its biological functions in ESCs are not elucidated. In this study, we find out that Brpf3 protein level is critical for Myst2 stability and E3 ligase Huwe1 functions as a novel negative regulator of Myst2 via ubiquitin-mediated degradation. Importantly, Brpf3 plays an antagonistic role in Huwe1-mediated degradation of Myst2, suggesting that protein–protein interaction between Brpf3 and Myst2 is required for retaining Myst2 stability. Further, Brpf3 overexpression causes the aberrant upregulation of Myst2 protein levels which in turn induces the dysregulated cell-cycle progression and also delay of early embryonic development processes such as embryoid-body formation and lineage commitment of mouse ESCs. The Brpf3 overexpression-induced phenotypes can be reverted by Huwe1 overexpression. Together, these results may provide novel insights into understanding the functions of Brpf3 in proper differentiation as well as cell-cycle progression of ESCs via regulation of Myst2 stability by obstructing Huwe1-mediated ubiquitination. In addition, we suggest that this is a useful report which sheds light on the function of an unknown gene in ESC field.

scholarly journals Heat Shock 70-kDa Protein 8 Isoform 1 Is Expressed on the Surface of Human Embryonic Stem Cells and Downregulated upon Differentiation

Stem Cells ◽  
2005 ◽  
Vol 23 (10) ◽  
pp. 1502-1513 ◽  
Author(s):  
Yeon Sung Son ◽  
Jae Hyun Park ◽  
Young Kook Kang ◽  
Jin-Sung Park ◽  
Hong Seo Choi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Heat Shock ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem
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