scholarly journals Hair cells with heterogeneous transfer characteristics encode mechanical stimuli in the lateral line of zebrafish

2018 ◽  
Author(s):  
Paul Pichler ◽  
Leon Lagnado
Keyword(s):  
Lateral Line ◽  
Hair Cells ◽  
Glutamate Release ◽  
Mechanical Stimuli ◽  
Initial Stimulus ◽  
Transfer Characteristics ◽  
Preferred Direction ◽  
Ribbon Synapses ◽  
Mechanical Information ◽  
Unique Signal

Ribbon synapses of hair cells transmit mechanical information but the transfer characteristics relating deflection of the hair bundle to glutamate release have not been assessed directly. Here we have imaged glutamate to investigate how hair cells encode information in the lateral line of zebrafish. Half the hair cells signalled cupula motion in either direction from rest, achieving maximum sensitivity for deflections of ~40 nm in the preferred direction. The remainder rectified completely and were less sensitive, extending the operating range of the neuromast beyond 1μm. Adaptation was also heterogeneous, with some hair cells generating sustained synaptic outputs and others transient. A unique signal encoded a return to rest: a transient burst from hair cells unresponsive to the initial stimulus. A mixed population of hair cells with these various transfer characteristics will allow a neuromast to encode weak stimuli as well as the amplitude and duration of stronger deflections.

scholarly journals Insights into Electroreceptor Development and Evolution from Molecular Comparisons with Hair Cells

2018 ◽  
Vol 58 (2) ◽  
pp. 329-340 ◽  
Author(s):  
Clare V H Baker ◽  
Melinda S Modrell
Keyword(s):  
Gene Expression ◽  
Hair Cell ◽  
Electric Fields ◽  
Lateral Line ◽  
Hair Cells ◽  
Water Movement ◽  
Low Frequency ◽  
Lateral Line System ◽  
Line System ◽  
Ribbon Synapses

Abstract The vertebrate lateral line system comprises a mechanosensory division, with neuromasts containing hair cells that detect local water movement (“distant touch”); and an electrosensory division, with electrosensory organs that detect the weak, low-frequency electric fields surrounding other animals in water (primarily used for hunting). The entire lateral line system was lost in the amniote lineage with the transition to fully terrestrial life; the electrosensory division was lost independently in several lineages, including the ancestors of frogs and of teleost fishes. (Electroreception with different characteristics subsequently evolved independently within two teleost lineages.) Recent gene expression studies in a non-teleost actinopterygian fish suggest that electroreceptor ribbon synapses employ the same transmission mechanisms as hair cell ribbon synapses, and show that developing electrosensory organs express transcription factors essential for hair cell development, including Atoh1 and Pou4f3. Previous hypotheses for electroreceptor evolution suggest either that electroreceptors and hair cells evolved independently in the vertebrate ancestor from a common ciliated secondary cell, or that electroreceptors evolved from hair cells. The close developmental and putative physiological similarities implied by the gene expression data support the latter hypothesis, i.e., that electroreceptors evolved in the vertebrate ancestor as a “sister cell-type” to lateral line hair cells.

scholarly journals Motor behaviour selectively inhibits hair cells activated by forward motion in the lateral line of Zebrafish

2019 ◽  
Author(s):  
Paul Pichler ◽  
Leon Lagnado
Keyword(s):  
Motor Neurons ◽  
Lateral Line ◽  
Hair Cells ◽  
Glutamate Release ◽  
Selective Inhibition ◽  
External World ◽  
The Body ◽  
Motor Behaviour ◽  
Cellular Mechanisms ◽  
Pressure Changes

SummaryHow do sensory systems disambiguate events in the external world from signals generated by the motor behaviour of the animal? One strategy is to suppress the sensory input whenever the motor system is active, but the cellular mechanisms remain unclear. We investigated how motor behaviour modulates signals transmitted by the lateral line of zebrafish, which senses pressure changes around the body of the animal. Activation of motor neurons during fictive swimming caused co-activation of efferent fibers and suppression of synaptic transmission from the primary mechanoreceptors, the hair cells. In some hair cells, a single motor spike inhibited glutamate release by about 50% and block was often complete within 50-100 ms of the start of swimming. All hair cells polarized to be activated by posterior deflections, as would occur during forward swimming, were suppressed by >90%, while only half of those polarized in the anterior direction were inhibited and by an average of just 45%. The selective inhibition of hair cells activated during motor behaviour provides a mechanism for the suppression of self-generated signals while maintaining sensitivity to stimuli originating in the external world.

scholarly journals The lhfpl5 ohnologs lhfpl5a and lhfpl5b are required for mechanotransduction in distinct populations of sensory hair cells in zebrafish

2019 ◽  
Author(s):  
Timothy Erickson ◽  
Itallia V. Pacentine ◽  
Alexandra Venuto ◽  
Rachel Clemens ◽  
Teresa Nicolson
Keyword(s):  
Inner Ear ◽  
Lateral Line ◽  
Hair Cells ◽  
Vestibular Function ◽  
Fusion Partner ◽  
Primary Role ◽  
Mechanical Stimuli ◽  
Vestibular Hair Cells ◽  
Lateral Line Organ ◽  
Line Organ

1AbstractHair cells sense and transmit auditory, vestibular, and hydrodynamic information by converting mechanical stimuli into electrical signals. This process of mechano-electrical transduction (MET) requires a mechanically-gated channel localized in the apical stereocilia of hair cells. In mice, lipoma HMGIC fusion partner-like 5 (LHFPL5) acts as an auxiliary subunit of the MET channel whose primary role is to correctly localize PCDH15 and TMC1 to the mechanotransduction complex. Zebrafish have two lhfpl5 genes (lhfpl5a and lhfpl5b), but their individual contributions to MET channel assembly and function have not been analyzed.Here we show that the zebrafish lhfpl5 genes are expressed in discrete populations of hair cells: lhfpl5a expression is restricted to auditory and vestibular hair cells in the inner ear, while lhfpl5b expression is specific to hair cells of the lateral line organ. Consequently, lhfpl5a mutants exhibit defects in auditory and vestibular function, while disruption of lhfpl5b affects hair cells only in the lateral line neuromasts. In contrast to previous reports in mice, localization of Tmc1 does not depend upon Lhfpl5 function in either the inner ear or lateral line organ. In both lhfpl5a and lhfpl5b mutants, GFP-tagged Tmc1 and Tmc2b proteins still localize to the stereocilia of hair cells. Using a stably integrated GFP-Lhfpl5a transgene, we show that the tip link cadherins Pcdh15a and Cdh23, along with the Myo7aa motor protein, are required for correct Lhfpl5a localization at the tips of stereocilia. Our work corroborates the evolutionarily conserved co-dependence between Lhfpl5 and Pcdh15, but also reveals novel requirements for Cdh23 and Myo7aa to correctly localize Lhfpl5a. In addition, our data suggest that targeting of Tmc1 and Tmc2b proteins to stereocilia in zebrafish hair cells occurs independently of Lhfpl5 proteins.

scholarly journals Anion Exchanger 1b in Stereocilia Is Required for the Functioning of Mechanotransducer Channels in Lateral-Line Hair Cells of Zebrafish

PLoS ONE ◽  
2015 ◽  
Vol 10 (2) ◽  
pp. e0117041 ◽  
Author(s):  
Yuan-Hsiang Lin ◽  
Giun-Yi Hung ◽  
Liang-Chun Wu ◽  
Sheng-Wen Chen ◽  
Li-Yih Lin ◽  
...  
Keyword(s):  
Lateral Line ◽  
Hair Cells ◽  
Anion Exchanger

S-100 protein is a selective marker for sensory hair cells of the lateral line system in teleosts

2002 ◽  
Vol 329 (2) ◽  
pp. 133-136 ◽  
Author(s):  
F Abbate ◽  
S Catania ◽  
A Germanà ◽  
T González ◽  
B Diaz-Esnal ◽  
...  
Keyword(s):  
Lateral Line ◽  
Hair Cells ◽  
Lateral Line System ◽  
Selective Marker ◽  
Line System ◽  
Sensory Hair ◽  
Sensory Hair Cells ◽  
S 100

Extracellular Receptor Potentials from the Lateral-Line Organ of Xenopus Laevis

1980 ◽  
Vol 86 (1) ◽  
pp. 63-77
Author(s):  
ALFONS B. A. KROESE ◽  
JOHAN M. VAN DER ZALM ◽  
JOEP VAN DEN BERCKEN
Keyword(s):  
Xenopus Laevis ◽  
Lateral Line ◽  
Hair Cells ◽  
Water Displacement ◽  
Stimulus Amplitude ◽  
Nerve Fibres ◽  
Large Stimulus ◽  
Sensory Hair ◽  
Lateral Line Organ ◽  
Line Organ

1. The response of the epidermal lateral-line organ of Xenopus laevis to stimulation was studied by recording extracellular receptor potentials from the hair cells in single neuromasts in isolated preparations. One neuromast was stimulated by local, sinusoidal water movements induced by a glass sphere positioned at a short distance from the neuromast. 2. The amplitudes of the extracellular receptor potentials were proportional to the stimulus amplitude over a range of 20 dB. The phase of the extracellular receptor potentials with respect to water displacement was independent of the stimulus amplitude. 3. With large stimulus amplitude, and stimulus frequencies between 0.5 Hz and 2 Hz, the extracellular receptor potentials, and responses of single afferent nerve fibres, showed a phase lead of 1.2 π radians with respect to water displacement, i.e. they were almost in phase with water acceleration. 4. It is concluded that under conditions of stimulation with small-amplitude water movements, the hair cells respond to sensory hair displacement, whereas under conditions of stimulation with large-amplitude water movements they respond to sensory hair velocity.

scholarly journals Morphology of lateral line canals in Neotropical freshwater stingrays (Chondrichthyes: Potamotrygonidae) from Negro River, Brazilian Amazon

2010 ◽  
Vol 8 (4) ◽  
pp. 867-876 ◽  
Author(s):  
Akemi Shibuya ◽  
Jansen Zuanon ◽  
Maria Lúcia G. de Araújo ◽  
Sho Tanaka
Keyword(s):  
Lateral Line ◽  
The Body ◽  
Mechanical Stimuli ◽  
High Concentration ◽  
Ventral Region ◽  
Negro River ◽  
Accurate Localization ◽  
Posterior Lateral Line ◽  
The Family ◽  
The Relationship

The relationship between the distribution of the lateral line canals and their functionality has not been well examined in elasmobranchs, especially among Neotropical freshwater stingrays of the family Potamotrygonidae. The spatial distribution of the canals and their tubules and the quantification of the neuromasts were analyzed in preserved specimens of Potamotrygon motoro, P. orbignyi, Potamotrygon sp. "cururu", and Paratrygon aiereba from the middle Negro River, Amazonas, Brazil. The hyomandibular, infraorbital, posterior lateral line, mandibular, nasal and supraorbital canals were characterized and their pores and neuromasts quantified. The ventral canals are known to facilitate the accurate localization of prey items under the body, and our results indicate that the dorsal canals may be employed in identifying the presence of predators or potential prey positioned above the stingray's body. The presence of non-pored canals in the ventral region may be compensated by the high concentration of neuromasts found in the same area, which possibly allow the accurate detection of mechanical stimuli. The concentration of non-pored canals near the mouth indicates their importance in locating and capturing prey buried in the bottom substrate, possibly aided by the presence of vesicles of Savi.

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