scholarly journals An epigenetic breeding system in soybean for increased yield and stability

2017 ◽  
Author(s):  
Sunil Kumar Kenchanmane Raju ◽  
Mon-Ray Shao ◽  
Robersy Sanchez ◽  
Ying-Zhi Xu ◽  
Ajay Sandhu ◽  
...  
Keyword(s):  
Field Trials ◽  
Yield Stability ◽  
Specific Gene ◽  
Epigenetic Variation ◽  
Wild Type ◽  
Abiotic Stress Response ◽  
Abscisic Acid Biosynthesis ◽  
Plant Seed ◽  
Wide Range ◽  
Developmental Reprogramming

ABSTRACTEpigenetic variation has been associated with a wide range of adaptive phenotypes in plants, but there exist few direct means for exploiting this variation. RNAi suppression of the plant-specific gene,MutS HOMOLOG1(MSH1), in multiple plant species produces a range of developmental changes accompanied by modulation of defense, phytohormone, and abiotic stress response pathways. Thismsh1-conditioneddevelopmental reprogramming is retained independent of transgene segregation, giving rise to transgene-null ‘memory’ effects. An isogenic memory line crossed to wild type produces progeny families displaying increased variation in adaptive traits that respond to selection. This study investigates amenability of theMSH1system for inducing epigenetic variation in soybean that may be of value agronomically. We developed epi-line populations by crossing withmsh1-acquired soybean memory lines. Derived soybean epi-lines showed increase in variance for multiple yield-related traits including pods per plant, seed weight, and maturity time in both greenhouse and field trials. Selected epi-F2:4and epi-F2:5lines showed an increase in seed yield over wild type. By epi-F2:6, we observed a return of MSH1-derived enhanced growth back to wild type levels. Epi-populations also showed evidence of reduced epitype-by-environment (e × E) interaction, indicating higher yield stability. Transcript profiling of the soybean epi-lines identified putative signatures of enhanced growth behavior across generations. Genes related to cell cycle, abscisic acid biosynthesis, and auxin-response, particularly SMALL AUXIN UP RNAs (SAURs), were differentially expressed in epi-F2:4lines that showed increased yield when compared to epi-F2:6. These data support the potential ofmsh1-derived epigenetic variation in plant breeding for enhanced yield and yield stability.

Application of the Innovative and Non-Invasive Technique, Molecular Music Therapy (MMT), Bio-Frequency Therapy, for the Treatment of a Wide Range of Disorders and Pathologies, with Consequent Verification of Molecular Parameters by Using Bi-Digital O-Ring Test (BDORT)

2020 ◽  
Vol 44 (3) ◽  
pp. 177-189
Author(s):  
Momir Dunjic ◽  
Stefano Turini ◽  
Dejan Krstic ◽  
Katarina Dunjic ◽  
Marija Dunjic ◽  
...  
Keyword(s):  
Amino Acid ◽  
Music Therapy ◽  
Amino Acid Sequences ◽  
Sirtuin 1 ◽  
Ring Test ◽  
Invasive Technique ◽  
Specific Gene ◽  
Radiofrequency Therapy ◽  
Wide Range ◽  
Clinical Pictures

Radiofrequency therapy is an unconventional method, already applied for some time, with numerous results in numerous clinical pictures. Our group has developed a software, later called SONGENPROT-SOLARIS, capable of directly converting nucleotide sequences (DNA and/or RNA) and amino acid sequences (polypeptides and proteins) into musical sequences, based on mathematic matrices, designed by the French physicist and musician Joel Sternheimer, which allows to associate a musical note with a nucleotide or an amino acid. Innovation in our software is that, in the algorithm that defines it, a variant is directly implemented that allows the reproduction of sounds, phase-shifted by 30 Hz, between one ear and another reproducing the phenomenon of Binaural Tones, capable of induce a specific brain activity and also the release of particles called solitons. Thanks to this software we have developed a technique called MMT (Molecular Music Therapy) and currently, we are in the phase of applying the technique on a cohort of 91 patients, with a high spectrum of clinical pictures, examining the same, using the technique Bi-Digital-ORing-Test (BDORT), before and after treatment with MMT. Aim of project is to stimulate the expression of a specific gene (the same genetic sequence that the patient listens to, translated into music), only through the use of sound sequences. We have concentrated our attention on three main molecules: Sirtuin-1, Telomers and TP-53. The results obtained with BDORT, after treatment with MMT, showed a significant increase in the values of the three molecules, on all the examined patients, demonstrating the operative efficacy of the technique and the its applicability to numerous diseases. In order to confirm the data obtained by BDORT, we propose, with the help of an accredited laboratory, to perform epigenetic tests on the three parameters listed above, paving the way to understanding how frequencies can influence gene expression.

scholarly journals Transcriptome and metabolome profiling provide insights into molecular mechanism of pseudostem elongation in banana

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Guiming Deng ◽  
Fangcheng Bi ◽  
Jing Liu ◽  
Weidi He ◽  
Chunyu Li ◽  
...  
Keyword(s):  
Cell Wall ◽  
Cell Elongation ◽  
Molecular Mechanisms ◽  
Specific Gene ◽  
Wild Type ◽  
Banana Plant ◽  
Cell Wall Modification ◽  
Dwarf Cultivar ◽  
Important Trait ◽  
Metabolome Profiling

AbstractBackgroundBanana plant height is an important trait for horticultural practices and semi-dwarf cultivars show better resistance to damages by wind and rain. However, the molecular mechanisms controlling the pseudostem height remain poorly understood. Herein, we studied the molecular changes in the pseudostem of a semi-dwarf banana mutant Aifen No. 1 (Musaspp. Pisang Awak sub-group ABB) as compared to its wild-type dwarf cultivar using a combined transcriptome and metabolome approach.ResultsA total of 127 differentially expressed genes and 48 differentially accumulated metabolites were detected between the mutant and its wild type. Metabolites belonging to amino acid and its derivatives, flavonoids, lignans, coumarins, organic acids, and phenolic acids were up-regulated in the mutant. The transcriptome analysis showed the differential regulation of genes related to the gibberellin pathway, auxin transport, cell elongation, and cell wall modification. Based on the regulation of gibberellin and associated pathway-related genes, we discussed the involvement of gibberellins in pseudostem elongation in the mutant banana. Genes and metabolites associated with cell wall were explored and their involvement in cell extension is discussed.ConclusionsThe results suggest that gibberellins and associated pathways are possibly developing the observed semi-dwarf pseudostem phenotype together with cell elongation and cell wall modification. The findings increase the understanding of the mechanisms underlying banana stem height and provide new clues for further dissection of specific gene functions.

scholarly journals Genotype-Specific Signal Generation Based on Digestion of 3-Way DNA Junctions: Application to KRAS Variation Detection

2006 ◽  
Vol 52 (10) ◽  
pp. 1855-1863 ◽  
Author(s):  
Giulia Amicarelli ◽  
Daniel Adlerstein ◽  
Erlet Shehi ◽  
Fengfei Wang ◽  
G Mike Makrigiorgos
Keyword(s):  
Nucleic Acid ◽  
Concordance Rate ◽  
Signal Generation ◽  
Sequence Variants ◽  
Wild Type ◽  
Specific Signal ◽  
Novel Technology ◽  
Dna Junctions ◽  
Wide Range ◽  
Kras Codon

Abstract Background: Genotyping methods that reveal single-nucleotide differences are useful for a wide range of applications. We used digestion of 3-way DNA junctions in a novel technology, OneCutEventAmplificatioN (OCEAN) that allows sequence-specific signal generation and amplification. We combined OCEAN with peptide-nucleic-acid (PNA)-based variant enrichment to detect and simultaneously genotype v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS) codon 12 sequence variants in human tissue specimens. Materials and Methods: We analyzed KRAS codon 12 sequence variants in 106 lung cancer surgical specimens. We conducted a PNA-PCR reaction that suppresses wild-type KRAS amplification and genotyped the product with a set of OCEAN reactions carried out in fluorescence microplate format. The isothermal OCEAN assay enabled a 3-way DNA junction to form between the specific target nucleic acid, a fluorescently labeled “amplifier”, and an “anchor”. The amplifier-anchor contact contains the recognition site for a restriction enzyme. Digestion produces a cleaved amplifier and generation of a fluorescent signal. The cleaved amplifier dissociates from the 3-way DNA junction, allowing a new amplifier to bind and propagate the reaction. Results: The system detected and genotyped KRAS sequence variants down to ∼0.3% variant-to-wild-type alleles. PNA-PCR/OCEAN had a concordance rate with PNA-PCR/sequencing of 93% to 98%, depending on the exact implementation. Concordance rate with restriction endonuclease-mediated selective-PCR/sequencing was 89%. Conclusion: OCEAN is a practical and low-cost novel technology for sequence-specific signal generation. Reliable analysis of KRAS sequence alterations in human specimens circumvents the requirement for sequencing. Application is expected in genotyping KRAS codon 12 sequence variants in surgical specimens or in bodily fluids, as well as single-base variations and sequence alterations in other genes.

Grain mineral density of bread and durum wheat landraces from geochemically diverse native soils

2018 ◽  
Vol 69 (4) ◽  
pp. 335 ◽  
Author(s):  
José Francisco Vázquez ◽  
Efraín Antonio Chacón ◽  
José María Carrillo ◽  
Elena Benavente
Keyword(s):  
Durum Wheat ◽  
Triticum Aestivum L ◽  
Field Trials ◽  
Mineral Elements ◽  
Small Scale ◽  
Mineral Density ◽  
Native Soil ◽  
Wide Range ◽  
Grain Zinc ◽  
Wheat Landraces

Future progress on the creation of wheat cultivars with high grain zinc (Zn) and iron (Fe) mineral density will depend on both the availability of suitable donor germplasm and the identification of genes or quantitative trait loci contributing to increase the accumulation of mineral elements in the wheat kernels. Multi-environment field trials were conducted to evaluate the grain Zn, Fe and protein concentration of 32 bread wheat (Triticum aestivum L.) and 20 durum wheat (T. turgidum L. var. durum) landraces locally adapted to soils covering a wide range of pH values and mineral composition. These landraces were selected after a preliminary, small-scale field trial that had analysed 425 Spanish local varieties. Analyses of variance demonstrated a significant effect of genotype on grain composition, and 16 wheat landraces with elevated grain Zn and/or Fe density across the environments were identified. These landraces rich in grain minerals represent valuable primary gene-pool parents for wheat biofortification. No pattern of native soil geochemical characteristics that could help to predict the success in collecting mineral-dense genotypes in a given area was found. Mapping populations derived from some pairs of grain-mineral-rich and -poor genotypes characterised in the study may facilitate the development of molecular markers to assist the selection of superior wheat genotypes.

scholarly journals Functional Promiscuity of Gene Regulation by Serpentine Receptors in Dictyostelium discoideum

1998 ◽  
Vol 18 (10) ◽  
pp. 5744-5749 ◽  
Author(s):  
Irene Verkerke-Van Wijk ◽  
Ji-Yun Kim ◽  
Raymond Brandt ◽  
Peter N. Devreotes ◽  
Pauline Schaap
Keyword(s):  
Gene Expression ◽  
Cell Fate ◽  
Dictyostelium Discoideum ◽  
Developmental Regulation ◽  
Mutant Cell ◽  
Gene Induction ◽  
Specific Gene ◽  
Wild Type ◽  
Animal Development ◽  
Camp Stimulation

ABSTRACT Serpentine receptors such as smoothened and frizzled play important roles in cell fate determination during animal development. InDictyostelium discoideum, four serpentine cyclic AMP (cAMP) receptors (cARs) regulate expression of multiple classes of developmental genes. To understand their function, it is essential to know whether each cAR is coupled to a specific gene regulatory pathway or whether specificity results from the different developmental regulation of individual cARs. To distinguish between these possibilities, we measured gene induction in car1 car3 double mutant cell lines that express equal levels of either cAR1, cAR2, or cAR3 under a constitutive promoter. We found that all cARs efficiently mediate both aggregative gene induction by cAMP pulses and induction of postaggregative and prespore genes by persistent cAMP stimulation. Two exceptions to this functional promiscuity were observed. (i) Only cAR1 can mediate adenosine inhibition of cAMP-induced prespore gene expression, a phenomenon that was found earlier in wild-type cells. cAR1’s mediation of adenosine inhibition suggests that cAR1 normally mediates prespore gene induction. (ii) Only cAR2 allows entry into the prestalk pathway. Prestalk gene expression is induced by differentiation-inducing factor (DIF) but only after cells have been prestimulated with cAMP. We found that DIF-induced prestalk gene expression is 10 times higher in constitutive cAR2 expressors than in constitutive cAR1 or cAR3 expressors (which still have endogenous cAR2), suggesting that cAR2 mediates induction of DIF competence. Since in wild-type slugs cAR2 is expressed only in anterior cells, this could explain the so far puzzling observations that prestalk cells differentiate at the anterior region but that DIF levels are actually higher at the posterior region. After the initial induction of DIF competence, cAMP becomes a repressor of prestalk gene expression. This function can again be mediated by cAR1, cAR2, and cAR3.

scholarly journals In-field screening for host plant resistance to Delia radicum and Brevicoryne brassicae within selected rapeseed cultivars and new interspecific hybrids

2020 ◽  
Vol 15 (1) ◽  
pp. 711-720
Author(s):  
Janetta Niemann ◽  
Justyna Szwarc ◽  
Jan Bocianowski ◽  
Dorota Weigt ◽  
Marek Mrówczyński
Keyword(s):  
Life Sciences ◽  
Field Trials ◽  
Brevicoryne Brassicae ◽  
Delia Radicum ◽  
Sources Of Resistance ◽  
Cabbage Aphid ◽  
Hybrid Combination ◽  
Resistance To Insects ◽  
Wide Range ◽  
High Level

AbstractRapeseed (Brassica napus) can be attacked by a wide range of pests, for example, cabbage root fly (Delia radicum) and cabbage aphid (Brevicoryne brassicae). One of the best methods of pest management is breeding for insect resistance in rapeseed. Wild genotypes of Brassicaceae and rapeseed cultivars can be used as a source of resistance. In 2017, 2018, and 2019, field trials were performed to assess the level of resistance to D. radicum and B. brassicae within 53 registered rapeseed cultivars and 31 interspecific hybrid combinations originating from the resources of the Department of Genetics and Plant Breeding of Poznań University of Life Sciences (PULS). The level of resistance varied among genotypes and years. Only one hybrid combination and two B. napus cultivars maintained high level of resistance in all tested years, i.e., B. napus cv. Jet Neuf × B. carinata – PI 649096, Galileus, and Markolo. The results of this research indicate that resistance to insects is present in Brassicaceae family and can be transferred to rapeseed cultivars. The importance of continuous improvement of rapeseed pest resistance and the search for new sources of resistance is discussed; furthermore, plans for future investigations are presented.

Differential elimination of rDNA genes in bobbed mutants of Drosophila melanogaster

1986 ◽  
Vol 6 (4) ◽  
pp. 1023-1031
Author(s):  
R Terracol ◽  
N Prud'homme
Keyword(s):  
Drosophila Melanogaster ◽  
Gene Copy Number ◽  
Rdna Locus ◽  
Gene Copy ◽  
Specific Gene ◽  
Type I ◽  
28S Rrna ◽  
Wild Type ◽  
Y Chromosomes ◽  
Genes Encoding

In Drosophila melanogaster, the multiply repeated genes encoding 18S and 28S rRNA are located on the X and Y chromosomes. A large percentage of these repeats are interrupted in the 28S region by insertions of two types. We compared the restriction patterns from a subcloned wild-type Oregon R strain to those of spontaneous and ethyl methanesulfonate-induced bobbed mutants. Bobbed mutations were found to be deficiencies that modified the organization of the rDNA locus. Genes without insertions were deleted about twice as often as genes with type I insertions. Type II insertion genes were not decreased in number, except in the mutant having the most bobbed phenotype. Reversion to wild type was associated with an increase in gene copy number, affecting exclusively genes without insertions. One hypothesis which explains these results is the partial clustering of genes by type. The initial deletion could then be due either to an unequal crossover or to loss of material without exchange. Some of our findings indicated that deletion may be associated with an amplification phenomenon, the magnitude of which would be dependent on the amount of clustering of specific gene types at the locus.

Modifications of Seizure Susceptibility inDrosophila

2000 ◽  
Vol 83 (2) ◽  
pp. 998-1009 ◽  
Author(s):  
Daniel Kuebler ◽  
Mark A. Tanouye
Keyword(s):  
Idiopathic Epilepsy ◽  
Seizure Susceptibility ◽  
Wild Type ◽  
Physiological Basis ◽  
Seizure Disorders ◽  
Spontaneous Seizures ◽  
Wide Range ◽  
Symptomatic Seizures ◽  
Type Strains ◽  
Over Time

In a given population, certain individuals are much more likely to have seizures than others. This increase in seizure susceptibility can lead to spontaneous seizures, such as seen in idiopathic epilepsy, or to symptomatic seizures that occur after insults to the nervous system. Despite the frequency of these seizure disorders in the human population, the genetic and physiological basis for these defects remains unclear. The present study makes use of Drosophila as a potentially powerful model for understanding seizure susceptibility in humans. In addition to the genetic and molecular advantages of using Drosophila, it has been found that seizures in Drosophila share much in common with seizures seen in humans. However, the most powerful aspect of this model lies in the ability to accurately measure seizure susceptibility across genotypes and over time. In the current study seizure susceptibility was quantified in a variety of mutant and wild-type strains, and it was found that genetic mutations can modulate susceptibility over an extremely wide range. This genetic modulation of seizure susceptibility apparently occurs without affecting the threshold of individual neurons. Seizure susceptibility also varied depending on the experience of the fly, decreasing immediately after a seizure and then gradually increasing over time. A novel phenomenon was also identified in which seizures are suppressed after certain high-intensity stimuli. These results demonstrate the utility of Drosophila as a model system for studying human seizure disorders and provide insights into the possible mechanisms by which seizure susceptibility is modified.

scholarly journals Virulence and Metabolic Characteristics of Salmonella enterica Serovar Enteritidis Strains with DifferentsefDVariants in Hens

2012 ◽  
Vol 78 (18) ◽  
pp. 6405-6412 ◽  
Author(s):  
Cesar A. Morales ◽  
Jean Guard ◽  
Roxana Sanchez-Ingunza ◽  
Devendra H. Shah ◽  
Mark Harrison
Keyword(s):  
Salmonella Enterica ◽  
Egg Production ◽  
The Body ◽  
Wild Type ◽  
Salmonella Enterica Serovar Enteritidis ◽  
Blood Calcium ◽  
Content Type ◽  
Metabolic Characteristics ◽  
Wide Range ◽  
Metabolic Properties

ABSTRACTSalmonella entericaserovar Enteritidis is one of a fewSalmonella entericaserotypes that has SEF14 fimbriae encoded by thesefoperon, which consists of 4 cotranscribed genes,sefABCD, regulated bysefR. A parental strain was used to construct asefDmutant and its complement, and all 3 strains were compared for gene expression, metabolic properties, and virulence characteristics in hens. Transcription ofsefDby wild type was suppressed at 42°C and absent for the mutant under conditions where the complemented mutant had 103times higher transcription. Growth of the complemented mutant was restricted in comparison to that of the mutant and wild type. Hens infected with the wild type and mutant showed decreased blood calcium and egg production, but infection with the complemented mutant did not. Thus, the absence ofsefDcorrelated with increased metabolic capacity and enhanced virulence of the pathogen. These results suggest that any contribution thatsefDmakes to egg contamination is either unknown or would be limited to early transmission from the environment to the host. Absence ofsefD, either through mutation or by suppression of transcription at the body temperature of the host, may contribute to the virulence ofSalmonella entericaby facilitating growth on a wide range of metabolites.

Genetic characterization of Rhizobium sp. strain TAL1145 that nodulates tree legumes

1994 ◽  
Vol 40 (3) ◽  
pp. 208-215 ◽  
Author(s):  
M. L. C. George ◽  
J. P. W. Young ◽  
D. Borthakur
Keyword(s):  
Rhizobium Leguminosarum ◽  
Genetic Characterization ◽  
Rhizobium Meliloti ◽  
Wild Type ◽  
Tree Legumes ◽  
Wide Range ◽  
The Common ◽  
Rrna Sequences ◽  
Rhizobium Sp

Rhizobium sp. strain TALI 145 nodulates Leucaena ieucocephaia and Phaseolus vulgaris, in addition to a wide range of tropical tree legumes. Six overlapping clones that complemented nodulation defects in leucaena and bean rhizobia were isolated and a 40-kb map of the symbiosis region was constructed. The common nod and nifA genes were situated approximately 17 kb apart, with the nodlJ genes in between. These clones enabled a derivative of TAL1145 carrying a partially deleted pSym to form ineffective nodules on both leucaena and bean, and a similar derivative of Rhizobium etli TAL182 to form ineffective nodules on bean. When two representative clones, pUHR9 and pUHR114, were each transferred to wild-type rhizobial strains, they allowed ineffective nodulation by Rhizobium meliloti on both leucaena and bean and by Rhizobium leguminosarum bv. viciae on bean. Transconjugants of R. leguminosarum bv. trifolii formed effective nodules on leucaena and ineffective nodules on bean. Tn5 mutagenesis of the symbiosis region resulted in a variety of nodulation and fixation phenotypes on leucaena and bean. On the basis of 16S rRNA sequences, TAL1145 was found to be distinct from both R. tropici and NGR234, the two groups of leucaena symbionts that were previously described.Key words: Rhizobium, Leucaena leucocephala, nodulation, nitrogen fixation.

Sign in / Sign up

Export Citation Format

Share Document