scholarly journals High genomic diversity of multi-drug resistant wastewater Escherichia coli

2017 ◽  
Author(s):  
Norhan Mahfouz ◽  
Serena Caucci ◽  
Eric Achatz ◽  
Torsten Semmler ◽  
Sebastian Guenther ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Wastewater Treatment ◽  
Wastewater Treatment Plants ◽  
Genotypic Variation ◽  
Treatment Plant ◽  
Multidrug Resistant ◽  
Genomic Diversity ◽  
Drug Resistant ◽  
Large Dataset ◽  
Whole Genomes

AbstractWastewater treatment plants play an important role in the release of antibiotic resistance into the environment. It has been shown that wastewater contains multi-drug resistant Escherichia coli, but information on strain diversity is surprisingly scarce. Here we present an exceptionally large dataset on multidrug resistant Escherichia coli, originating from wastewater, over a thousand isolates were phenotypically characterized for twenty antibiotics and for 103 isolates whole genomes were sequenced. To our knowledge this is the first study documenting such a comprehensive diversity of multi-drug resistant Escherichia coli in wastewater. The genomic diversity of the isolates was unexpectedly high and contained a high number of resistance and virulence genes. To illustrate the genomic diversity of the isolates we calculated the pan genome of the wastewater Escherichia coli and found it to contain over sixteen thousand genes. To analyse this diverse dataset, we devised a computational approach correlating genotypic variation and resistance phenotype, this way we were able to identify not only known, but also candidate resistance genes. Finally, we could verify that the effluent of a wastewater treatment plant will contain multi-drug resistant Escherichia coli belonging to clinically important clonal groups.

Removal improvement of bacteria (Escherichia coli and enterococci) in maturation ponds using baffles

2012 ◽  
Vol 65 (4) ◽  
pp. 589-595 ◽  
Author(s):  
A. Ouali ◽  
H. Jupsin ◽  
J. L. Vasel ◽  
L. Marouani ◽  
A. Ghrabi
Keyword(s):  
Escherichia Coli ◽  
Wastewater Treatment ◽  
Wastewater Treatment Plants ◽  
Treatment Plant ◽  
E Coli ◽  
Conventional Activated Sludge ◽  
In Series ◽  
Hydrodynamic Study ◽  
Rhodamine Wt ◽  
Engineering Intervention

Korba wastewater treatment plant is a conventional activated sludge followed by three maturation ponds (MP1, MP2, MP3) in series acting as a tertiary treatment. The first study of wastewater treatment plants showed that the effluent concentration of Escherichia coli and enterococci at the outlet of the (MP3) varies between 103 and 104CFU/100 ml. After the hydrodynamic study conducted by Rhodamine WT which showed short-circuiting in the MP1, two baffles were introduced in the first maturation pond (MP1) to improve the hydrodynamic and the sanitary performances. The second hydraulic study showed that the dispersion number ‘d’ was reduced from 1.45 to 0.43 by this engineering intervention and the Peclet number was raised from 0.69 to 2.32. The hydraulic retention time was increased by 14 h. Because of well-designed baffles, the removal efficiency of E. coli and enterococci was raised between 0.2 and 0.7 log units for the first maturation pond.

scholarly journals Genomic Insights of Multidrug-Resistant Escherichia coli From Wastewater Sources and Their Association With Clinical Pathogens in South Africa

2021 ◽  
Vol 8 ◽  
Author(s):  
Joshua Mbanga ◽  
Daniel G. Amoako ◽  
Akebe L. K. Abia ◽  
Mushal Allam ◽  
Arshad Ismail ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Virulence Genes ◽  
Treatment Plant ◽  
Multidrug Resistant ◽  
Genomic Diversity ◽  
Health Concern ◽  
Comparative Genomic ◽  
Phylogenomic Analysis ◽  
E Coli ◽  
Wwtp Effluent

There is limited information on the comparative genomic diversity of antibiotic-resistant Escherichia coli from wastewater. We sought to characterize environmental E. coli isolates belonging to various pathotypes obtained from a wastewater treatment plant (WWTP) and its receiving waters using whole-genome sequencing (WGS) and an array of bioinformatics tools to elucidate the resistomes, virulomes, mobilomes, clonality, and phylogenies. Twelve multidrug-resistant (MDR) diarrheagenic E. coli isolates were obtained from the final effluent of a WWTP, and the receiving river upstream and downstream of the WWTP were sequenced on an Illumina MiSeq machine. The multilocus sequence typing (MLST) analysis revealed that multiple sequence types (STs), the most common of which was ST69 (n = 4) and ST10 (n = 2), followed by singletons belonging to ST372, ST101, ST569, ST218, and ST200. One isolate was assigned to a novel ST ST11351. A total of 66.7% isolates were positive for β-lactamase genes with 58.3% harboring the blaTEM1B gene and a single isolate the blaCTX−M−14 and blaCTX−M−55 extended-spectrum β-lactamase (ESBL) genes. One isolate was positive for the mcr-9 mobilized colistin resistance gene. Most antibiotic resistance genes (ARGs) were associated with mobile genetic support: class 1 integrons (In22, In54, In191, and In369), insertion sequences (ISs), and/or transposons (Tn402 or Tn21). A total of 31 virulence genes were identified across the study isolates, including those responsible for adhesion (lpfA, iha, and aggR), immunity (air, gad, and iss), and toxins (senB, vat, astA, and sat). The virulence genes were mostly associated with IS (IS1, IS3, IS91, IS66, IS630, and IS481) or prophages. Co-resistance to heavy metal/biocide, antibiotics were evident in several isolates. The phylogenomic analysis with South African E. coli isolates from different sources (animals, birds, and humans) revealed that isolates from this study mostly clustered with clinical isolates. Phylogenetics linked with metadata revealed that isolates did not cluster according to source but according to ST. The occurrence of pathogenic and MDR isolates in the WWTP effluent and the associated river is a public health concern.

scholarly journals Virulence and plasmidic resistance determinants of Escherichia coli isolated from municipal and hospital wastewater treatment plants

2014 ◽  
Vol 13 (2) ◽  
pp. 311-318 ◽  
Author(s):  
Vera Calhau ◽  
Catarina Mendes ◽  
Angelina Pena ◽  
Nuno Mendonça ◽  
Gabriela Jorge Da Silva
Keyword(s):  
Escherichia Coli ◽  
Wastewater Treatment ◽  
Wastewater Treatment Plants ◽  
Multidrug Resistant ◽  
Quinolone Resistance ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Hospital Wastewater ◽  
Aminoglycoside Resistance ◽  
E Coli

Escherichia coli is simultaneously an indicator of water contamination and a human pathogen. This study aimed to characterize the virulence and resistance of E. coli from municipal and hospital wastewater treatment plants (WWTPs) in central Portugal. From a total of 193 isolates showing reduced susceptibility to cefotaxime and/or nalidixic acid, 20 E. coli with genetically distinct fingerprint profiles were selected and characterized. Resistance to antimicrobials was determined using the disc diffusion method. Extended spectrum β-lactamase and plasmid-mediated quinolone resistance genes, phylogroups, pathogenicity islands (PAIs) and virulence genes were screened by polymerase chain reaction (PCR). CTX-M producers were typed by multilocus sequence typing. Resistance to beta-lactams was associated with the presence of blaTEM,blaSHV, blaCTX-M-15 and blaCTX-M-32. Plasmid-mediated quinolone resistance was associated with qnrA, qnrS and aac(6′)-Ib-cr. Aminoglycoside resistance and multidrug-resistant phenotypes were also detected. PAI IV536, PAI IICFT073, PAI II536 and PAI ICFT073, and uropathogenic genes iutA, papAH and sfa/foc were detected. With regard to the clinical ST131 clone, it carried blaCTX-M-15, blaTEM-type, qnrS and aac(6′)-lb-cr; IncF and IncP plasmids, and virulence factors PAI IV536, PAI ICFT073, PAI IICFT073, iutA, sfa/foc and papAH were identified in the effluent of a hospital plant. WWTPs contribute to the dissemination of virulent and resistant bacteria in water ecosystems, constituting an environmental and public health risk.

scholarly journals Big Data Analytics from a Wastewater Treatment Plant

2021 ◽  
Vol 13 (22) ◽  
pp. 12383
Author(s):  
Praewa Wongburi ◽  
Jae K. Park
Keyword(s):  
Big Data ◽  
Wastewater Treatment ◽  
Data Analytics ◽  
Wastewater Treatment Plants ◽  
Treatment Plant ◽  
Big Data Analytics ◽  
Operational Performance ◽  
Clear Understanding ◽  
Large Dataset ◽  
Regulatory Limits

Wastewater treatment plants (WWTPs) use considerable workforces and resources to meet the regulatory limits without mistakes. The advancement of information technology allowed for collecting large amounts of data from various sources using sophisticated sensors. Due to the lack of specialized tools and knowledge, operators and engineers cannot effectively extract meaningful and valuable information from large datasets. Unfortunately, the data are often stored digitally and then underutilized. Various data analytics techniques have been developed in the past few years. The methods are efficient for analyzing vast datasets. However, there is no wholly developed study in applying these techniques to assist wastewater treatment operation. Data analytics processes can immensely transform a large dataset into informative knowledge, such as hidden information, operational problems, or even a predictive model. The use of big data analytics will allow operators to have a much clear understanding of the operational status while saving the operation and maintenance costs and reducing the human resources required. Ultimately, the method can be applied to enhance the operational performance of the wastewater treatment infrastructure.

High prevalence of multidrug-resistant Escherichia coli and Enterococcus spp. in river water, upstream and downstream of a wastewater treatment plant

2014 ◽  
Vol 12 (3) ◽  
pp. 426-435 ◽  
Author(s):  
Lucinda J. Bessa ◽  
Ana Barbosa-Vasconcelos ◽  
Ângelo Mendes ◽  
Paulo Vaz-Pires ◽  
Paulo Martins da Costa
Keyword(s):  
Escherichia Coli ◽  
Wastewater Treatment ◽  
Antimicrobial Resistance ◽  
River Water ◽  
Wastewater Treatment Plant ◽  
Treatment Plant ◽  
Multidrug Resistant ◽  
Antimicrobial Drugs ◽  
E Coli ◽  
Enterococcus Spp

In this study, microbial quality and antimicrobial resistance of faecal bacteria from a Portuguese river were assessed. River water samples collected upstream and downstream of a wastewater treatment plant, throughout a 3-month period, were used for the enumeration of Escherichia coli and Enterococcus spp. The highest numbers found for E. coli and enterococci were 1.1 × 104 and 1.2 × 104 colony forming units (CFU)/100 ml, respectively. In total, 144 isolates of E. coli and 144 of enterococci were recovered and tested for antimicrobial susceptibility; 104 E. coli and 78 Enterococcus spp. showed resistance to one or more antimicrobial drugs. Overall, 70 and 32 different resistance patterns were found for E. coli and enterococci, respectively. One E. coli showed resistance to imipenem and 29 isolates were extended spectrum β-lactamase-producers. Multidrug-resistant E. coli belonged mostly to groups A, B1 and group D. Enterococcus spp. were mostly resistant to rifampicin, tetracycline, azithromycin and erythromycin; six isolates showed resistance to vancomycin, presenting the VanA phenotype. The high levels of E. coli and enterococci and the remarkable variety of antimicrobial resistance profiles, reinforces the theory that these river waters can be a pool of antimicrobial resistance determinants, which can be easily spread among different bacteria and reach other environments and hosts.

scholarly journals Emergence of High Level Carbapenem and Extensively Drug Resistant Escherichia coli ST746 Producing NDM-5 in Influent of Wastewater Treatment Plant, Seoul, South Korea

2021 ◽  
Vol 12 ◽  
Author(s):  
Hanseob Shin ◽  
Yeonghyeon Kim ◽  
Dukki Han ◽  
Hor-Gil Hur
Keyword(s):  
Escherichia Coli ◽  
Wastewater Treatment ◽  
South Korea ◽  
Resistance Genes ◽  
Treatment Plant ◽  
Carbapenem Resistance ◽  
Drug Resistant ◽  
E Coli ◽  
Extensively Drug Resistant ◽  
High Level

High level carbapenem and extensively drug resistant (XDR) Escherichia coli strain N7, which produces a variant of New Delhi metallo-β-lactamase (NDM-5), was isolated from the influent of the Jungnang wastewater treatment plant located on Han River, Seoul, South Korea. Phenotypic and genotypic resistances to carbapenem were tested using agar and broth dilution methods, and polymerase chain reaction. Whole-genome sequencing was performed to characterize the genetic structure of strain N7. E. coli strain N7, which harbors the blaNDM–5 gene, showed high level of carbapenem resistance at concentrations of doripenem (512 mg/L) and meropenem (256 mg/L), and XDR to 15 antibiotics. Based on the genomic sequence analysis, two plasmids, a hybrid IncHI2/N-type and an IncX3 type, were present. The former contains a cluster (blaNDM–5-bleMBL-trpF-dsbD) bracketed by multi-insertional sequences, IS3000, ISAba125, IS5, and IS26. The latter carries the following resistance genes: blaCTX–14, aac(3)-IV, aadA1, aadA2, aph(3′)-Ia, aph(4)-Ia, sul1, sul2, sul3, dfrA12, fosA3, oqxA, oqxB, mph(A), and floR, and cmlA1. The chromosome, contig3, and contig5 also carry blaCTX–64 and mdf(A), tet(A), and erm(B), tet(M) and aadA22, respectively. Strain N7 also harbors virulence factors such as fimH, flu, ecpABCDE, sfmA, hlyE, and gadA. This study demonstrates the emergence of high level carbapenem resistant XDR E. coli strain N7 containing blaNDM–5 in aquatic environment, Seoul, South Korea. Due to the presence of mobile genetic elements, this strain could horizontally transfer resistance genes, including blaNDM–5 to environmental bacteria. Thus, it is necessary to conduct continuous surveillance for carbapenem resistance in various aquatic environments.

Analysis and balance of phosphorous removal from wastewater at urban wastewater treatment plant

2020 ◽  
Vol 15 (2) ◽  
pp. 142-151
Author(s):  
Peter Lukac ◽  
Lubos Jurik
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plant ◽  
Wastewater Treatment Plants ◽  
Sewage Treatment ◽  
Sewage Treatment Plant ◽  
Treatment Plant ◽  
Phosphorus Recovery ◽  
Anaerobic Sludge ◽  
Biological Phosphorus Removal ◽  
The Mean

Abstract:Phosphorus is a major substance that is needed especially for agricultural production or for the industry. At the same time it is an important component of wastewater. At present, the waste management priority is recycling and this requirement is also transferred to wastewater treatment plants. Substances in wastewater can be recovered and utilized. In Europe (in Germany and Austria already legally binding), access to phosphorus-containing sewage treatment is changing. This paper dealt with the issue of phosphorus on the sewage treatment plant in Nitra. There are several industrial areas in Nitra where record major producers in phosphorus production in sewage. The new wastewater treatment plant is built as a mechanicalbiological wastewater treatment plant with simultaneous nitrification and denitrification, sludge regeneration, an anaerobic zone for biological phosphorus removal at the beginning of the process and chemical phosphorus precipitation. The sludge management is anaerobic sludge stabilization with heating and mechanical dewatering of stabilized sludge and gas management. The aim of the work was to document the phosphorus balance in all parts of the wastewater treatment plant - from the inflow of raw water to the outflow of purified water and the production of excess sludge. Balancing quantities in the wastewater treatment plant treatment processes provide information where efficient phosphorus recovery could be possible. The mean daily value of P tot is approximately 122.3 kg/day of these two sources. The mean daily value of P tot is approximately 122.3 kg/day of these two sources. There are also two outflows - drainage of cleaned water to the recipient - the river Nitra - 9.9 kg Ptot/day and Ptot content in sewage sludge - about 120.3 kg Ptot/day - total 130.2 kg Ptot/day.

Sludge minimization by disintegration at different wastewater treatment plants

2008 ◽  
Vol 3 (1) ◽  
Author(s):  
Luchien Luning ◽  
Paul Roeleveld ◽  
Victor W.M. Claessen
Keyword(s):  
Organic Matter ◽  
Wastewater Treatment ◽  
Anaerobic Digestion ◽  
Sewage Sludge ◽  
New Technologies ◽  
Wastewater Treatment Plants ◽  
Treatment Plant ◽  
Biological Degradation ◽  
Ongoing Research ◽  
Sludge Minimization

In recent years new technologies have been developed to improve the biological degradation of sewage sludge by anaerobic digestion. The paper describes the results of a demonstration of ultrasonic disintegration on the Dutch Wastewater Treatment Plant (WWTP) Land van Cuijk. The effect on the degradation of organic matter is presented, together with the effect on the dewatering characteristics. Recommendations are presented for establishing research conditions in which the effect of sludge disintegration can be determined in a more direct way that is less sensitive to changing conditions in the operation of the WWTP. These recommendations have been implemented in the ongoing research in the Netherlands supported by the National Institute for wastewater research (STOWA).

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