scholarly journals Phosphorylation of the synaptonemal complex protein SYP-1 promotes meiotic chromosome segregation

2017 ◽  
Author(s):  
Aya Sato-Carlton ◽  
Chihiro Nakamura-Tabuchi ◽  
Stephane Kazuki Chartrand ◽  
Tomoki Uchino ◽  
Peter Mark Carlton
Keyword(s):  
Synaptonemal Complex ◽  
Meiotic Chromosome ◽  
C Elegans ◽  
Chromosome Domains ◽  
Chromatid Cohesion ◽  
Complex Protein ◽  
Chromosomal Passenger ◽  
Synaptonemal Complex Protein ◽  
A Site ◽  
Meiosis I

AbstractChromosomes that have undergone crossing-over in meiotic prophase must maintain sister chromatid cohesion somewhere along their length between the first and second meiotic divisions. While many eukaryotes use the centromere as a site to maintain cohesion, the holocentric organism C. elegans instead creates two chromosome domains of unequal length termed the short arm and long arm, which become the first and second site of cohesion loss at meiosis I and II. The mechanisms that confer distinct functions to the short and long arm domains remain poorly understood. Here, we show that phosphorylation of the synaptonemal complex protein SYP-1 is required to create these domains. Once crossovers are made, phosphorylated SYP-1 and PLK-2 become cooperatively confined to short arms and guide phosphorylated histone H3 and the chromosomal passenger complex to the site of meiosis I cohesion loss. Our results show that PLK-2 and phosphorylated SYP-1 ensure creation of the short arm subdomain, promoting disjunction of chromosomes in meiosis I.

scholarly journals Phosphorylation of the synaptonemal complex protein SYP-1 promotes meiotic chromosome segregation

2017 ◽  
Vol 217 (2) ◽  
pp. 555-570 ◽  
Author(s):  
Aya Sato-Carlton ◽  
Chihiro Nakamura-Tabuchi ◽  
Stephane Kazuki Chartrand ◽  
Tomoki Uchino ◽  
Peter Mark Carlton
Keyword(s):  
Synaptonemal Complex ◽  
Meiotic Chromosome ◽  
Chromosome Domains ◽  
Chromatid Cohesion ◽  
Complex Protein ◽  
Chromosomal Passenger ◽  
Synaptonemal Complex Protein ◽  
A Site ◽  
Unequal Length ◽  
Meiosis I

Chromosomes that have undergone crossing over in meiotic prophase must maintain sister chromatid cohesion somewhere along their length between the first and second meiotic divisions. Although many eukaryotes use the centromere as a site to maintain cohesion, the holocentric organism Caenorhabditis elegans instead creates two chromosome domains of unequal length termed the short arm and long arm, which become the first and second site of cohesion loss at meiosis I and II. The mechanisms that confer distinct functions to the short and long arm domains remain poorly understood. Here, we show that phosphorylation of the synaptonemal complex protein SYP-1 is required to create these domains. Once crossover sites are designated, phosphorylated SYP-1 and PLK-2 become cooperatively confined to short arms and guide phosphorylated histone H3 and the chromosomal passenger complex to the site of meiosis I cohesion loss. Our results show that PLK-2 and phosphorylated SYP-1 ensure creation of the short arm subdomain, promoting disjunction of chromosomes in meiosis I.

scholarly journals The Synaptonemal Complex Protein Zip1 Promotes Bi-Orientation of Centromeres at Meiosis I

PLoS Genetics ◽  
2009 ◽  
Vol 5 (12) ◽  
pp. e1000771 ◽  
Author(s):  
Mara N. Gladstone ◽  
David Obeso ◽  
Hoa Chuong ◽  
Dean S. Dawson
Keyword(s):  
Synaptonemal Complex ◽  
Complex Protein ◽  
Synaptonemal Complex Protein ◽  
Meiosis I

scholarly journals The synaptonemal complex protein, Zip1, promotes the segregation of nonexchange chromosomes at meiosis I

2009 ◽  
Vol 107 (2) ◽  
pp. 781-785 ◽  
Author(s):  
L. Newnham ◽  
P. Jordan ◽  
B. Rockmill ◽  
G. S. Roeder ◽  
E. Hoffmann
Keyword(s):  
Synaptonemal Complex ◽  
Complex Protein ◽  
Synaptonemal Complex Protein ◽  
Meiosis I

ZIP1 is a synaptonemal complex protein required for meiotic chromosome synapsis

Cell ◽  
1993 ◽  
Vol 72 (3) ◽  
pp. 365-378 ◽  
Author(s):  
Mary Sym ◽  
JoAnne Engebrecht ◽  
G.Shirleen Roeder
Keyword(s):  
Synaptonemal Complex ◽  
Meiotic Chromosome ◽  
Chromosome Synapsis ◽  
Complex Protein ◽  
Synaptonemal Complex Protein

scholarly journals Polo-like kinase-dependent phosphorylation of the synaptonemal complex protein SYP-4 regulates double-strand break formation through a negative feedback loop.

eLife ◽  
2017 ◽  
Vol 6 ◽  
Author(s):  
Saravanapriah Nadarajan ◽  
Talley J Lambert ◽  
Elisabeth Altendorfer ◽  
Jinmin Gao ◽  
Michael D Blower ◽  
...  
Keyword(s):  
Synaptonemal Complex ◽  
Central Region ◽  
Feedback Loop ◽  
Strand Break ◽  
Dynamic State ◽  
Strand Breaks ◽  
Homologous Chromosomes ◽  
C Elegans ◽  
Complex Protein ◽  
Synaptonemal Complex Protein

The synaptonemal complex (SC) is an ultrastructurally conserved proteinaceous structure that holds homologous chromosomes together and is required for the stabilization of pairing interactions and the completion of crossover (CO) formation between homologs during meiosis I. Here, we identify a novel role for a central region component of the SC, SYP-4, in negatively regulating formation of recombination-initiating double-strand breaks (DSBs) via a feedback loop triggered by crossover designation in C. elegans. We found that SYP-4 is phosphorylated dependent on Polo-like kinases PLK-1/2. SYP-4 phosphorylation depends on DSB formation and crossover designation, is required for stabilizing the SC in pachytene by switching the central region of the SC from a more dynamic to a less dynamic state, and negatively regulates DSB formation. We propose a model in which Polo-like kinases recognize crossover designation and phosphorylate SYP-4 thereby stabilizing the SC and making chromosomes less permissive for further DSB formation.

scholarly journals Double or nothing: a Drosophila mutation affecting meiotic chromosome segregation in both females and males.

Genetics ◽  
1994 ◽  
Vol 136 (3) ◽  
pp. 953-964 ◽  
Author(s):  
D P Moore ◽  
W Y Miyazaki ◽  
J E Tomkiel ◽  
T L Orr-Weaver
Keyword(s):  
Cell Death ◽  
Chromosome Segregation ◽  
Sister Chromatid ◽  
Meiotic Chromosome ◽  
Sister Chromatid Cohesion ◽  
Aberrant Chromosome ◽  
Chromatid Cohesion ◽  
Meiotic Nondisjunction ◽  
Lethal Allele ◽  
Meiosis I

Abstract We describe a Drosophila mutation, Double or nothing (Dub), that causes meiotic nondisjunction in a conditional, dominant manner. Previously isolated mutations in Drosophila specifically affect meiosis either in females or males, with the exception of the mei-S332 and ord genes which are required for proper sister-chromatid cohesion. Dub is unusual in that it causes aberrant chromosome segregation almost exclusively in meiosis I in both sexes. In Dub mutant females both nonexchange and exchange chromosomes undergo nondisjunction, but the effect of Dub on nonexchange chromosomes is more pronounced. Dub reduces recombination levels slightly. Multiple nondisjoined chromosomes frequently cosegregate to the same pole. Dub results in nondisjunction of all chromosomes in meiosis I of males, although the levels are lower than in females. When homozygous, Dub is a conditional lethal allele and exhibits phenotypes consistent with cell death.

scholarly journals X-ray crystallographic studies of the middle part of the human synaptonemal complex protein 1 coiled-coil domain

2015 ◽  
Vol 71 (9) ◽  
pp. 1131-1134 ◽  
Author(s):  
Hyun Ho Park
Keyword(s):  
Synaptonemal Complex ◽  
Short Form ◽  
Complex Structure ◽  
Coiled Coil ◽  
Middle Part ◽  
X Ray ◽  
Cell Parameters ◽  
Coiled Coil Domain ◽  
Complex Protein ◽  
Synaptonemal Complex Protein

The synaptonemal complex is a meiosis-specific complex structure formed at the synapse of homologous chromosomes to hold them together during meiosis. Synaptonemal complex protein 1 (SYCP1) is one of the components of the syneptonemal complex. In this study, the short form of the coiled-coil domain of SYCP1 was overexpressed inEscherichia coliwith an engineered C-terminal His tag. The short form of the coiled-coil domain of SYCP1 was then purified to homogeneity and crystallized at 293 K. X-ray diffraction data were collected to a resolution of 3.0 Å from a crystal belonging to space groupI4, with unit-cell parametersa= 41.95,b= 41.95,c= 318.78 Å. The asymmetric unit was estimated to contain two molecules.

scholarly journals Synaptonemal complex protein 2 (SYCP2) mediates the association of the centromere with the synaptonemal complex

2017 ◽  
Vol 8 (7) ◽  
pp. 538-543 ◽  
Author(s):  
Jianrong Feng ◽  
Shijuan Fu ◽  
Xuan Cao ◽  
Hao Wu ◽  
Jing Lu ◽  
...  
Keyword(s):  
Synaptonemal Complex ◽  
Complex Protein ◽  
Synaptonemal Complex Protein
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