scholarly journals FLIM-based pH measurements reveal incretin-induced rejuvenation of aged insulin secretory granules

2017 ◽  
Author(s):  
Martin Neukam ◽  
Anke Sönmez ◽  
Michele Solimena
Keyword(s):  
Secretory Granules ◽  
Population Level ◽  
Underlying Mechanism ◽  
Fusion Pore ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
Luminal Ph ◽  
Relationship Of ◽  
First Time ◽  
The Relationship

AbstractInsulin is stored in dense-core secretory granules (SGs) and is released from beta cells in two distinct phases upon glucose stimulation. Newly synthesized insulin SGs are secreted preferentially, but the underlying mechanism of this phenomenon remains elusive. The relationship of SG age with their intraluminal pH is of particular interest: proinsulin conversion by prohormone convertases follows the acidification of immature SGs by the vacuolar proton-translocating ATPase (v-ATPase). v-ATPases may also participate in the formation of the fusion pore for SG exocytosis, with intraluminal alkalinization inhibiting membrane fusion. Previous studies examined the luminal pH of SGs on a population level only. Here we measured the pH-dependent lifetime changes of eCFP fused to the ICA512-RESP18 homology domain to assess for the first time the luminal pH of individual age-defined SGs in insulinoma INS-1 cells by fluorescence-lifetime imaging microscopy. We show that 2-4-hour-old young SGs have a pH of ~5.5, while 26-28-hour-old SGs have a pH of ~6.2. Remarkably, the GLP-1 receptor agonist Exendin-4 prompted the re-acidification of old SGs in a glutamate-dependent fashion, while it did not affect the pH of young SGs. This study demonstrates that insulin SGs change their pH over time - a change that is reversible by insulin secretagogues. Hence, it provides novel insight into the mechanisms accounting for aging and exocytosis of SGs and suggests that their ‘rejuvenation’ may be exploited to enhance insulin secretion in diabetes.

ЗУБРЫ НА СЕВЕРЕ РОССИИ — НАСТОЯЩЕЕ И БУДУЩЕЕ

2019 ◽  
pp. 42-44
Author(s):  
I.V. GUSAROV ◽  
V.A. OSTAPENKO ◽  
T.V. NOVIKOVА
Keyword(s):  
Biological Diversity ◽  
Climatic Conditions ◽  
Economic Consequences ◽  
European Bison ◽  
Rare And Endangered Species ◽  
The North ◽  
Rare And Endangered ◽  
Relationship Of ◽  
First Time ◽  
The Relationship

Впервые в мире создана популяция зубров на территории 60 градусов северной широты. В новых климатических условиях разведения и сохранения зубров определены и проанализированы факторы существования вида на севере Европейской части РФ. Выявлены признаки, динамика численности, которые являются составной частью системы, предназначенной для управления биоразнообразием. Интродукция, являясь процессом введения в экосистему нехарактерных для нее видов, может усиливать изменения биоценозов как положительно, так и отрицательно. Насколько быстро и успешно проходит процесс адаптации заселенного вида, и усматривается его влияние на окружающую среду зависит дальнейшее существование зубров и в целом биоразнообразия. В статье обсуждаются вопросы взаимоотношения зубров с другими видами копытных и хозяйственной деятельностью человека, а также дальнейшим использованием зубров в сельскохозяйственном производстве. Пластичность зубров, выявление изменений и их анализ при вселении видов в новые условия обитания необходимы не только для определения развития или деградации биоценозов и в целом экосистемы, но и прогноза социально-экономических последствий интродукции как одного из методов сохранения редких и исчезающих видов фауны.For the first time in the world, a bison population has been created in an area of 60 degrees north latitude. In the new climatic conditions of breeding and preservation of bison, the factors of the species existence in the north of the European part of the Russian Federation are identified and analyzed. The signs, dynamics of abundance, which are an integral part of the system designed to manage biodiversity are identified, since the preservation of biological diversity on the planet is one of the main problems of our time. Introduction, being the process of introducing non-typical species into an ecosystem, can enhance changes in biocenoses, both positively and negatively. The question posing sounds especially when it comes to such a large hoofed animal as the European bison. How quickly and successfully the process of adaptation of the universe takes place and its environmental impact is seen depends on the continued existence of bison and biodiversity in general. The article discusses the relationship of bison with other types of ungulates and human activities, as well as the further use of bison in agricultural production. How these issues will be resolved positively depends on the future of these animals. Thus, the plasticity of bison, the identification of changes and their analysis, with the introduction of species into new habitat conditions is necessary not only to determine the development or degradation of biocenoses and the ecosystem as a whole, but also to predict the socio-economic consequences due to the introduction as one of the methods of preserving rare and endangered species of fauna.

“Quelques misérables places à remplir”Locating Shaped Painting in Eighteenth-Century France

2017 ◽  
Vol 80 (4) ◽  
pp. 544-571
Author(s):  
David Pullins
Keyword(s):  
Eighteenth Century ◽  
Visual Field ◽  
Decorative Arts ◽  
Relationship Of ◽  
First Time ◽  
The Relationship

Abstract This article addresses in depth for the first time the irregularly shaped canvases known as tableaux chantournés (cut-out paintings) that were produced in vast numbers by leading academicians between the 1730s and 1750s and occupy a tenuous place between fine and applied or decorative arts. Through an examination of the term’s first uses in regard to painting and eighteenth-century critics’ responses to these works, tableaux chantournés are positioned as a means of rethinking the extraction of painting from a richer visual field and the relationship of this medium-specific agenda to the historiography of the rococo.

scholarly journals Investigating hot-Jupiter inflated radii with hierarchical Bayesian modelling

2018 ◽  
Vol 616 ◽  
pp. A76 ◽  
Author(s):  
Marko Sestovic ◽  
Brice-Olivier Demory ◽  
Didier Queloz
Keyword(s):  
Large Fraction ◽  
Population Level ◽  
Mass Range ◽  
Hierarchical Bayesian ◽  
Incident Flux ◽  
Hot Jupiters ◽  
Probabilistic Relation ◽  
Relationship Of ◽  
The Relationship ◽  
Level Analysis

Context. As of today, hundreds of hot Jupiters have been found, yet the inflated radii of a large fraction of them remain unexplained. A number of mechanisms have been proposed to explain these anomalous radii, however most of these can only work under certain conditions and may not be sufficient to explain the most extreme cases. It is still unclear whether a single mechanism can sufficiently explain the entire distribution of radii, or whether a combination of these mechanisms is needed. Aims. We seek to understand the relationship of radius with stellar irradiation and mass and to find the range of masses over which hot Jupiters are inflated. We also aim to find the intrinsic physical scatter in their radii, caused by unobservable parameters, and to constrain the fraction of hot Jupiters that exhibit inflation. Methods. By constructing a hierarchical Bayesian model, we inferred the probabilistic relation between planet radius, mass, and incident flux for a sample of 286 gas giants. We separately incorporated the observational uncertainties of the data and the intrinsic physical scatter in the population. This allowed us to treat the intrinsic physical scatter in radii, due to latent parameters such as the heavy element fraction, as a parameter to be inferred. Results. We find that the planetary mass plays a key role in the inflation extent and that planets in the range ~0.37−0.98  MJ show the most inflated radii. At higher masses, the radius response to incident flux begins to decrease. Below a threshold of 0.37 ± 0.03  MJ we find that giant exoplanets as a population are unable to maintain inflated radii ≿1.4  RJ but instead exhibit smaller sizes as the incident flux is increased beyond 106 W m−2. We also find that below 1  MJ, there is a cut-off point at high incident flux beyond which we find no more inflated planets, and that this cut-off point decreases as the mass decreases. At incident fluxes higher than ~1.6 × 106 W m−2 and in a mass range 0.37−0.98  MJ, we find no evidence for a population of non-inflated hot Jupiters. Our study sheds a fresh light on one of the key questions in the field and demonstrates the importance of population-level analysis to grasp the underlying properties of exoplanets.

Hitherto Unprinted Manuscripts of the Middle English Ipotis

PMLA ◽  
1916 ◽  
Vol 31 (1) ◽  
pp. 114-160
Author(s):  
Josephine D. Sutton
Keyword(s):  
Middle English ◽  
Fourteenth Century ◽  
English Poem ◽  
Relationship Of ◽  
First Time ◽  
New Evidence ◽  
The Relationship

The relationship of the manuscripts of the Middle-English poem Ipotis has been studied in detail by Dr. Hugo Gruber on the basis of the nine mss. known to him. In addition to these there are five others, four of which are printed for the first time below. One of these, unfortunately a fragment, is of the greatest importance, since it carries back the date of the poem at least fifty years. On the basis of the earliest manuscript known to him—ms. Vernon, written about 1385—Gruber assigned the Ipotis to the second half of the fourteenth century. But in the light of the new evidence, the composition of the poem is pushed back to the very beginning of the century.

scholarly journals HOW TO SABOTAGE A SECRET SOCIETY: THE DEMISE OF CARL FRIEDRICH BAHRDT'S GERMAN UNION IN 1789

2017 ◽  
Vol 61 (2) ◽  
pp. 379-402
Author(s):  
ANDREW MCKENZIE-MCHARG
Keyword(s):  
Secret Society ◽  
Republic Of Letters ◽  
The Public ◽  
The North ◽  
State And Society ◽  
German Republic ◽  
The Status ◽  
Relationship Of ◽  
First Time ◽  
The Relationship

AbstractIn 1789 in Leipzig, a slim pamphlet of 128 pages appeared that sent shock waves through the German republic of letters. The pamphlet, bearing the title Mehr Noten als Text (More notes than text), was an ‘exposure’ whose most sensational element was a list naming numerous members of the North German intelligentsia as initiates of a secret society. This secret society, known as the German Union, aimed to push back against anti-Enlightenment tendencies most obviously manifest in the policies promulgated under the new Prussian king Frederick William II. The German Union was the brainchild of the notorious theologian Carl Friedrich Bahrdt (1741–92). But who was responsible for the ‘exposure’? Using material culled from several archives, this article pieces together for the first time the back story to Mehr Noten als Text and in doing so uncovers a surprisingly heterogeneous network of Freemasons, publishers, and state officials. The findings prompt us to reconsider general questions about the relationship of state and society in the late Enlightenment, the interplay of the public and the arcane spheres and the status of religious heterodoxy at this time.

scholarly journals Microscopic Fungi in Big Cities: Biodiversity, Source, and Relation to Pollution by Potentially Toxic Metals

Atmosphere ◽  
2021 ◽  
Vol 12 (11) ◽  
pp. 1471
Author(s):  
Maria V. Korneykova ◽  
Anastasia S. Soshina ◽  
Andrey I. Novikov ◽  
Kristina V. Ivashchenko ◽  
Olesya I. Sazonova ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Toxic Metals ◽  
Road Dust ◽  
Airborne Fungi ◽  
Potentially Toxic Metals ◽  
Opportunistic Fungi ◽  
Air Quality Assessment ◽  
Relationship Of ◽  
First Time ◽  
The Relationship

For the first time, a mycological analysis of outdoor urban environment (air, leaves, sealed surfaces) was carried in the cities of subarctic (Murmansk) and temperate (Moscow) climatic zones. The chemical composition of dust deposited on leaves of dominant tree species was taken as an indicator of the air quality. Assessment of the complex impact of factors (climate zone, type of substrate, anthropogenic load) on the quantitative and qualitative parameters of mycobiome was performed. Compared to Moscow, Murmansk was characterized by an increased number and concentrations of pollutants in the deposited dust. The number of culturable airborne fungi in Murmansk was substantially lower than in Moscow. Half of the species belonged to the opportunistic in both cities. Most dangerous opportunistic fungi were absent in the air of recreational zones but present on leaves surface and in road dust in all assessed zones of the cities. Dust chemical composition affected the diversity of fungi species. While the relationship of biological parameters with concentration of potentially toxic metals was generally negative, Cd increased the fraction of opportunistic fungi in road dust. The study revealed an importance of substrate in determining the sensitivity of outdoor mycobiome to pollution and highlighted its biological characteristics sensitive to climate.

scholarly journals The specifics of interpretation of the history in the story by A. Doronin «A Wolf Ravine»

2021 ◽  
Vol 11 (1) ◽  
pp. 139-147
Author(s):  
S. V. Sheyanova ◽  
◽  
N. M. Yusupova ◽  
Keyword(s):  
Literary Criticism ◽  
Historical Narrative ◽  
Volga Region ◽  
Psychological States ◽  
The Real ◽  
The Subject ◽  
The Individual ◽  
Relationship Of ◽  
First Time ◽  
The Relationship

Introduction: at present the reader’s audience is particularly interested in creative experiments in which the historical fate of the Russian peasantry in the «turning» eras is artistically comprehended. The article is devoted to the study of the problem-thematic range of modern Mordovian historical prose. The subject of analysis is the peculiarity of the reception of the period of collectivization and dekulakization in the story by Erzyan prose writer A. Doronin «A Wolf Ravine». Objective: to reveal the features of the artistic reconstruction of the events of the 1930s, the modeling of the relationship between a man and society in the story by A. Doronin «A Wolf Ravine».Research materials: the story by A. Doronin «A Wolf Ravine». Results and novelty of the research: the historical story « A Wolf Ravine » for the first time becomes the object of scientific understanding and is introduced into the context of Finno-Ugric literary criticism. A. Doronin artistically interprets the real events and circumstances of the resettlement of dispossessed peasants of the Volga region to the uninhabited steppes of Kazakhstan. As a result of the study, we conclude that the actualization of this problem-thematic cluster is due to the creative concept of the historical writer; the individual author’s approach to the reconstruction of historical narrative can be traced in the writer’s desire to realistically reveal the relationship of personality and society in the tragic 1930s; to analyze intentions of people and of the psychological states of the characters. Problems of a sociopolitical nature, actualized in the story, are filled with philosophical, axiological content, and lead to a multi-faceted understanding of the «man and history» problem.

scholarly journals Characteristics of cows’ cicatricial metabolism of different linearity

2021 ◽  
Vol 213 (10) ◽  
pp. 75-80
Author(s):  
E. Razhina
Keyword(s):  
Statistical Data ◽  
Ph Value ◽  
Rumen Fluid ◽  
Point Of View ◽  
Ideal Line ◽  
Microsoft Office ◽  
Relationship Of ◽  
First Time ◽  
The Relationship ◽  
Veterinary Laboratory

Abstract. The aim of this work is to investigate the relationship between indicators of cicatricial digestion and linearity of cows. Methods. The research was carried out in the breeding enterprises of the Sverdlovsk region on the livestock of Ural-type cows. Cicatricial fluid was collected with an oropharyngeal rubber probe, and a wooden yaw was also used. Cicatricial content was assessed at the Chelyabinsk Interregional Veterinary Laboratory. The pH value was determined by the electrometric method, VFA – in the Markgam apparatus, ammonia – by the microdiffusion method. Ciliates – in Goryaev’s chamber and under a microscope, bacteria – under a microscope with the addition of sodium chloride solution. Statistical data processing was carried out in the Microsoft Office Excel 2010 program. Results. In terms of the number of ciliates and bacteria in the rumen fluid, the Montwick Chieftain cows, characterized by high productive qualities, had an advantage. In terms of pH and VFA, the Vis Back Ideal line was the best. There were no significant differences between the groups in terms of the amount of ammonia in the rumen. The best animals from the point of view of influence on cicatricial metabolism were the animals of the Vis Back Ideal and Montwick Chieftain lineage. Scientific novelty. Studies have shown that linearity is associated with cicatricial digestion in cows. The relationship of bull lines with indicators of cicatricial metabolism of cows has been determined for the first time. The food in the rumen is digested due to the action of bacteria, ciliates, and fungi. An environment has been created in the rumen for the active development of microflora. Bacteria are able to synthesize amino acids and vitamins. The proventriculus can contain up to 50 species of ciliates.

scholarly journals Adolescent to young adult longitudinal development of subcortical volumes in two European sites with four waves

2021 ◽  
Author(s):  
Lea L. Backhausen ◽  
Juliane H. Froehner ◽  
Herve Lemaitre ◽  
Eric Artiges ◽  
Marie-Laure Paillere-Martinot ◽  
...  
Keyword(s):  
Sex Differences ◽  
Brain Development ◽  
Adolescent Psychopathology ◽  
Control Procedure ◽  
Structural Development ◽  
Age Related ◽  
Subcortical Regions ◽  
Relationship Of ◽  
First Time ◽  
The Relationship

Adolescent subcortical structural brain development might underlie psychopathological symptoms, which often emerge in adolescence. At the same time, sex differences exist in psychopathology, which might be mirrored in underlying sex differences in structural development. However, previous studies showed inconsistencies in subcortical trajectories and potential sex differences. Therefore, we aimed to investigate the subcortical structural trajectories and their sex differences across adolescence using for the first time a single cohort design, the same quality control procedure, software and a general additive mixed modeling approach. We investigated two large European sites from ages 14 to 24 with 503 participants and 1408 total scans from France and Germany as part of the IMAGEN project including four waves of data acquisition. We found significantly larger volumes in males versus females in both sites and across all seven subcortical regions. Sex differences in age-related trajectories were observed across all regions in both sites. Our findings provide further evidence of sex differences in longitudinal adolescent brain development of subcortical regions and thus might eventually support the relationship of underlying brain development and different adolescent psychopathology in boys and girls.

scholarly journals Practices of Constructing Regional Identity in the Media Discourse of Tatarstan

2020 ◽  
pp. 52-66
Author(s):  
O. V. Ilyina ◽  
E. V. Kablukov
Keyword(s):  
Regional Identity ◽  
Cultural Identification ◽  
Media Discourse ◽  
Traditional Religion ◽  
Regional Center ◽  
The Media ◽  
Relationship Of ◽  
First Time ◽  
The Relationship ◽  
Empirical Material

The authors consider identity as a conditional discursive construct, the result of subjects of discourse identifications and offer their own methodology for its analysis. It is shown that regional identity can be represented in the form of a model based on spatial and temporal identifications that specify the coordinate system of reality in which there are residents of the region in question. The concept of space-time is complemented by a set of diverse thematic identifications, including economic, political, cultural, ethnic, religious, linguistic, etc. For the first time in the framework of the socio-constructionist paradigm, a discursive model of the regional identity of the inhabitants of Tatarstan is constructed in the article. The empirical material of the study is the corpus of texts of Tatarstan media for 2017-2019. It is shown that the spatial identification of Tatarstan people includes the practice of selecting, nominating and describing significant geographical objects, the practice of constructing relations of Tatarstan with other geographical objects. Particular attention is paid to the practice of constructing the capital status of a regional center, as well as the relationship of spatial and political identifications. Analyzing the practices of ethnic, religious, linguistic and cultural identification, the authors come to the conclusion that the identity of the Tatarstan people is ethnocentric: despite the declared multinationality and multi-confessionality of the region, Tatars as an ethnic group, Tatar language, Tatar culture and Islam as a traditional religion of the Tatars are of particular importance.

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