scholarly journals An Open-Source, Programmable Pneumatic Setup for Operation and Automated Control of Single- and Multi-Layer Microfluidic Devices

2017 ◽  
Author(s):  
Kara Brower ◽  
Robert Puccinelli ◽  
Craig J. Markin ◽  
Tyler C. Shimko ◽  
Scott A. Longwell ◽  
...  
Keyword(s):  
Open Source ◽  
High Throughput ◽  
Modular Design ◽  
Microfluidic Devices ◽  
Automated Control ◽  
Fluid Physics ◽  
Biochemical Measurements ◽  
On Chip ◽  
The Cost ◽  
Valve Actuation

AbstractMicrofluidic technologies have been used across diverse disciplines (e.g. high-throughput biological measurement, fluid physics, laboratory fluid manipulation) but widespread adoption has been limited due to the lack of openly disseminated resources that enable non-specialist labs to make and operate their own devices. Here, we report the open-source build of a pneumatic setup capable of operating both single and multilayer (Quake-style) microfluidic devices with programmable scripting automation. This setup can operate both simple and complex devices with 48 device valve control inputs and 18 sample inputs, with modular design for easy expansion, at a fraction of the cost of similar commercial solutions. We present a detailed step-by-step guide to building the pneumatic instrumentation, as well as instructions for custom device operation using our software, Geppetto, through an easy-to-use GUI for live on-chip valve actuation and a scripting system for experiment automation. We show robust valve actuation with near real-time software feedback and demonstrate use of the setup for high-throughput biochemical measurements on-chip. This open-source setup will enable specialists and novices alike to run microfluidic devices easily in their own laboratories. Specifications table

scholarly journals An open-source, programmable pneumatic setup for operation and automated control of single- and multi-layer microfluidic devices

HardwareX ◽  
2018 ◽  
Vol 3 ◽  
pp. 117-134 ◽  
Author(s):  
Kara Brower ◽  
Robert R. Puccinelli ◽  
Craig J. Markin ◽  
Tyler C. Shimko ◽  
Scott A. Longwell ◽  
...  
Keyword(s):  
Open Source ◽  
Microfluidic Devices ◽  
Automated Control

scholarly journals OPEN leaf: an open-source cloud-based phenotyping system for tracking dynamic changes at leaf-specific resolution in Arabidopsis

2021 ◽  
Author(s):  
Landon Gary Alan Swartz ◽  
Suxing Liu ◽  
Drew Dahlquist ◽  
Emily S Walter ◽  
Skyler Kramer ◽  
...  
Keyword(s):  
Open Source ◽  
High Throughput ◽  
Modular Design ◽  
Morphological Changes ◽  
Gene Families ◽  
Plant Phenotyping ◽  
Gene Redundancy ◽  
High Throughput Screens ◽  
Changes Over Time ◽  
Inducible Gene

The first draft of the Arabidopsis genome was released more than 20 years ago and despite intensive molecular research, more than 30% of Arabidopsis genes remained uncharacterized or without an assigned function. This is in part due to gene redundancy within gene families or the essential nature of genes, where their deletion results in lethality (i.e., the dark genome). High-throughput plant phenotyping (HTPP) offers an automated and unbiased approach to characterize subtle or transient phenotypes resulting from gene redundancy or inducible gene silencing; however, commercial HTPP platforms remain unaffordable. Here we describe the design and implementation of OPEN leaf, an open-source HTPP system with cloud connectivity and remote bilateral communication to facilitate data collection, sharing and processing. OPEN leaf, coupled with the SMART imaging processing package was able to consistently document and quantify dynamic morphological changes over time at the whole rosette level and also at leaf-specific resolution when plants experienced changes in nutrient availability. The modular design of OPEN leaf allows for additional sensor integration. Notably, our data demonstrate that VIS sensors remain underutilized and can be used in high-throughput screens to identify characterize previously unidentified phenotypes in a leaf-specific manner.

scholarly journals High-throughput organ-on-chip platform with integrated programmable fluid flow and real-time sensing for complex tissue models in drug development workflows

Lab on a Chip ◽  
2021 ◽  
Vol 21 (8) ◽  
pp. 1454-1474
Author(s):  
H. Azizgolshani ◽  
J. R. Coppeta ◽  
E. M. Vedula ◽  
E. E. Marr ◽  
B. P. Cain ◽  
...  
Keyword(s):  
Fluid Flow ◽  
Drug Development ◽  
Real Time ◽  
High Throughput ◽  
Microfluidic Devices ◽  
On Chip ◽  
Tissue Models

96 microfluidic devices with independent electrical readouts are coupled with 192 micropumps to make a high-throughput organ-on-chip platform.

scholarly journals A Scalable Unsegmented Multiport Memory for FPGA-Based Systems

2015 ◽  
Vol 2015 ◽  
pp. 1-12 ◽  
Author(s):  
Kevin R. Townsend ◽  
Osama G. Attia ◽  
Phillip H. Jones ◽  
Joseph Zambreno
Keyword(s):  
High Throughput ◽  
High Performance ◽  
Storage Capacity ◽  
Memory Access ◽  
Reconfigurable Architectures ◽  
Communication Overhead ◽  
Trade Offs ◽  
On Chip ◽  
Access Patterns ◽  
The Cost

On-chip multiport memory cores are crucial primitives for many modern high-performance reconfigurable architectures and multicore systems. Previous approaches for scaling memory cores come at the cost of operating frequency, communication overhead, and logic resources without increasing the storage capacity of the memory. In this paper, we present two approaches for designing multiport memory cores that are suitable for reconfigurable accelerators with substantial on-chip memory or complex communication. Our design approaches tackle these challenges by banking RAM blocks and utilizing interconnect networks which allows scaling without sacrificing logic resources. With banking, memory congestion is unavoidable and we evaluate our multiport memory cores under different memory access patterns to gain insights about different design trade-offs. We demonstrate our implementation with up to 256 memory ports using a Xilinx Virtex-7 FPGA. Our experimental results report high throughput memories with resource usage that scales with the number of ports.

FreeLoader: An Open Source Universal Testing Machine for High-Throughput Experimentation

2011 ◽  
Author(s):  
John R. Amend ◽  
Hod Lipson
Keyword(s):  
Open Source ◽  
High Throughput ◽  
Low Cost ◽  
Testing Machine ◽  
Universal Testing Machine ◽  
Universal Testing ◽  
High Throughput Experimentation ◽  
Compressive Loads ◽  
Order Of Magnitude ◽  
The Cost

We present a low cost, desktop size, open source, universal testing machine, designed for inexpensive high-throughput material testing. The tester can apply tensile and compressive loads up to 5 kN at rates ranging from 2 mm/min to 30 mm/min. Force measurements are achieved with ±1.8 N accuracy. The parts list for this machine represents an order of magnitude reduction in the cost per testing unit as compared to commercial systems. We describe the design and construction of the tester and validate its performance. The design, parts list, control software, and user manual are made available freely online under the open source BSD license.

Review of Microfluidic Devices for On-Chip Chemical Sensing

2016 ◽  
Vol 136 (6) ◽  
pp. 244-249
Author(s):  
Takahiro Watanabe ◽  
Fumihiro Sassa ◽  
Yoshitaka Yoshizumi ◽  
Hiroaki Suzuki
Keyword(s):  
Chemical Sensing ◽  
Microfluidic Devices ◽  
On Chip

scholarly journals O23: CHARACTERISING PATIENT-DERIVED COLORECTAL CANCER TISSUE-ORIGINATED ORGANOIDAL SPHEROIDS FOR HIGH-THROUGHPUT MICROFLUIDIC APPLICATIONS

2021 ◽  
Vol 108 (Supplement_1) ◽  
Author(s):  
MI Khot ◽  
M Levenstein ◽  
R Coppo ◽  
J Kondo ◽  
M Inoue ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Fluid Flow ◽  
High Throughput ◽  
Microfluidic Devices ◽  
In Vitro Models ◽  
Fluorescent Imaging ◽  
Cancer Tissue ◽  
Cell Models

Abstract Introduction Three-dimensional (3D) cell models have gained reputation as better representations of in vivo cancers as compared to monolayered cultures. Recently, patient tumour tissue-derived organoids have advanced the scope of complex in vitro models, by allowing patient-specific tumour cultures to be generated for developing new medicines and patient-tailored treatments. Integrating 3D cell and organoid culturing into microfluidics, can streamline traditional protocols and allow complex and precise high-throughput experiments to be performed with ease. Method Patient-derived colorectal cancer tissue-originated organoidal spheroids (CTOS) cultures were acquired from Kyoto University, Japan. CTOS were cultured in Matrigel and stem-cell media. CTOS were treated with 5-fluorouracil and cytotoxicity evaluated via fluorescent imaging and ATP assay. CTOS were embedded, sectioned and subjected to H&E staining and immunofluorescence for ABCG2 and Ki67 proteins. HT29 colorectal cancer spheroids were produced on microfluidic devices using cell suspensions and subjected to 5-fluorouracil treatment via fluid flow. Cytotoxicity was evaluated through fluorescent imaging and LDH assay. Result 5-fluorouracil dose-dependent reduction in cell viability was observed in CTOS cultures (p<0.01). Colorectal CTOS cultures retained the histology, tissue architecture and protein expression of the colonic epithelial structure. Uniform 3D HT29 spheroids were generated in the microfluidic devices. 5-fluorouracil treatment of spheroids and cytotoxic analysis was achieved conveniently through fluid flow. Conclusion Patient-derived CTOS are better complex models of in vivo cancers than 3D cell models and can improve the clinical translation of novel treatments. Microfluidics can streamline high-throughput screening and reduce the practical difficulties of conventional organoid and 3D cell culturing. Take-home message Organoids are the most advanced in vitro models of clinical cancers. Microfluidics can streamline and improve traditional laboratory experiments.

scholarly journals Demonstration of an integrated nanophotonic chip-scale alkali vapor magnetometer using inverse design

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Yoel Sebbag ◽  
Eliran Talker ◽  
Alex Naiman ◽  
Yefim Barash ◽  
Uriel Levy
Keyword(s):  
Spatial Resolution ◽  
Near Field ◽  
Computer Assisted ◽  
Experimental Characterization ◽  
Optical Isolation ◽  
New Concepts ◽  
On Chip ◽  
The Cost ◽  
Micrometer Scale

AbstractRecently, there has been growing interest in the miniaturization and integration of atomic-based quantum technologies. In addition to the obvious advantages brought by such integration in facilitating mass production, reducing the footprint, and reducing the cost, the flexibility offered by on-chip integration enables the development of new concepts and capabilities. In particular, recent advanced techniques based on computer-assisted optimization algorithms enable the development of newly engineered photonic structures with unconventional functionalities. Taking this concept further, we hereby demonstrate the design, fabrication, and experimental characterization of an integrated nanophotonic-atomic chip magnetometer based on alkali vapor with a micrometer-scale spatial resolution and a magnetic sensitivity of 700 pT/√Hz. The presented platform paves the way for future applications using integrated photonic–atomic chips, including high-spatial-resolution magnetometry, near-field vectorial imaging, magnetically induced switching, and optical isolation.

High-throughput concentration of rare malignant tumor cells from large-volume effusions by multistage inertial microfluidics

Lab on a Chip ◽  
2022 ◽  
Author(s):  
Nan Xiang ◽  
Zhonghua Ni
Keyword(s):  
Tumor Cells ◽  
High Throughput ◽  
Malignant Tumor ◽  
Large Volume ◽  
Concentration Factor ◽  
Pleural Effusions ◽  
Inertial Microfluidics ◽  
Low Concentration ◽  
Malignant Pleural Effusions ◽  
On Chip

On-chip concentration of rare malignant tumor cells (MTCs) in malignant pleural effusions (MPEs) with a large volume is challenging. Previous microfluidic concentrators suffer from a low concentration factor (CF) and...

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