scholarly journals The fallacy of identification by neutralization in the light of cytopathology nonproducing enterovirus strains

2017 ◽  
Author(s):  
Moses Olubusuyi Adewumi ◽  
Temitope Oluwasegun Cephas Faleye ◽  
Johnson Adekunle Adeniji
Keyword(s):  
Molecular Characterization ◽  
Molecular Analysis ◽  
Specific Antisera ◽  
Southwest Nigeria ◽  
Coxsackievirus A24 ◽  
Structural Region

ABSTRACTWe describe the characterization of an enterovirus isolate recovered from untreated raw sewage in Ibadan, southwest Nigeria in 2010. The isolate was neutralized by specific antisera and consequently identified as Echovirus 7 (E7). Subsequent molecular characterization showed the isolate to be a mixture of E7 and Coxsackievirus A24 (CV-A24) thereby suggesting the CV-A24 strain to be non-cytopathology producing. Further molecular analysis suggested that the CV-A24 might have recombined with a Sabin poliovirus 2 (PV2) in its non-structural region. This is the first description of a Nigerian CV-A24 strain.

scholarly journals Molecular characterization of Prunus necrotic ringspot virus isolated from rose in Brazil

2015 ◽  
Vol 45 (12) ◽  
pp. 2197-2200 ◽  
Author(s):  
Thor Vinícius Martins Fajardo ◽  
Monique Bezerra Nascimento ◽  
Marcelo Eiras ◽  
Osmar Nickel ◽  
Gilvan Pio-Ribeiro
Keyword(s):  
Amino Acid ◽  
Molecular Characterization ◽  
Molecular Analysis ◽  
Nucleotide Sequences ◽  
Amino Acid Sequences ◽  
Causal Agent ◽  
Ringspot Virus ◽  
Prunus Necrotic Ringspot Virus ◽  
First Time

ABSTRACT: There is no molecular characterization of Brazilian isolates of Prunus necrotic ringspot virus (PNRSV), except for those infecting peach. In this research, the causal agent of rose mosaic was determined and the movement (MP) and coat (CP) protein genes of a PNRSV isolate from rose were molecularly characterized for the first time in Brazil. The nucleotide and deduced amino acid sequences of MP and CP complete genes were aligned and compared with other isolates. Molecular analysis of the MP and CP nucleotide sequences of a Brazilian PNRSV isolate from rose and others from this same host showed highest identities of 96.7% and 98.6%, respectively, and Rose-Br isolate was classified in PV32 group.

scholarly journals Renal Tumors of Childhood—A Histopathologic Pattern-Based Diagnostic Approach

Cancers ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 729 ◽  
Author(s):  
Ariadne H.A.G. Ooms ◽  
Gordan M. Vujanić ◽  
Ellen D’Hooghe ◽  
Paola Collini ◽  
Aurore L’Herminé-Coulomb ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Molecular Analysis ◽  
Pediatric Oncology ◽  
Correct Diagnosis ◽  
Renal Tumors ◽  
Pediatric Tumors ◽  
Cell Type ◽  
Appropriate Treatment ◽  
Histologic Pattern

Renal tumors comprise approximately 7% of all malignant pediatric tumors. This is a highly heterogeneous group of tumors, each with its own therapeutic management, outcome, and association with germline predispositions. Histopathology is the key in establishing the correct diagnosis, and therefore pathologists with expertise in pediatric oncology are needed for dealing with these rare tumors. While each tumor shows different histologic features, they do have considerable overlap in cell type and histologic pattern, making the diagnosis difficult to establish, if based on routine histology alone. To this end, ancillary techniques, such as immunohistochemistry and molecular analysis, can be of great importance for the correct diagnosis, resulting in appropriate treatment. To use ancillary techniques cost-effectively, we propose a pattern-based approach and provide recommendations to aid in deciding which panel of antibodies, supplemented by molecular characterization of a subset of genes, are required.

scholarly journals Molecular Characterization of Chelonid Alphaherpesvirus 5 in a Black Turtle (Chelonia mydas) Fibropapilloma from Baja California Sur, Mexico

Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 105
Author(s):  
Eduardo Reséndiz ◽  
Helena Fernández-Sanz ◽  
José Francisco Domínguez-Contreras ◽  
Amelly Hyldaí Ramos-Díaz ◽  
Agnese Mancini ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Molecular Analysis ◽  
Baja California ◽  
Clinical Signs ◽  
Chelonia Mydas ◽  
Baja California Sur ◽  
Epidermal Hyperplasia ◽  
Routine Monitoring ◽  
Hematological Values

During routine monitoring in Ojo de Liebre Lagoon, Mexico, a juvenile black turtle (Chelonia mydas) was captured, physically examined, measured, weighed, sampled, and tagged. The turtle showed no clinical signs suggestive of disease. Eleven months later, this turtle was recaptured in the same area, during which one lesion suggestive of fibropapilloma on the neck was identified and sampled for histopathology and molecular analysis. Histopathology revealed hyperkeratosis, epidermal hyperplasia, acanthosis, papillary differentiation and ballooning degeneration of epidermal cells, increased fibroblasts in the dermis, and angiogenesis, among other things. Hematological values were similar to those reported for clinically healthy black turtles and did not show notable changes between the first capture and the recapture; likewise, clinicopathological evaluation did not show structural or functional damage in the turtle’s systems. The chelonid alphaherpesvirus 5 (ChHV5) UL30 gene was amplified and sequenced for phylogeny; Bayesian reconstruction showed a high alignment with the genus Scutavirus of the Eastern Pacific group. This is one of the first reports of ChHV5 in a cutaneous fibropapilloma of a black turtle in the Baja California peninsula.

scholarly journals Molecular Characterization of Moroccan Fig Germplasm Using Intersimple Sequence Repeat and Simple Sequence Repeat Markers To Establish A Reference Collection

HortScience ◽  
2005 ◽  
Vol 40 (1) ◽  
pp. 29-32 ◽  
Author(s):  
B. Khadari ◽  
A. Oukabli ◽  
M. Ater ◽  
A. Mamouni ◽  
J.P. Roger ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Molecular Characterization ◽  
Molecular Analysis ◽  
Simple Sequence Repeat ◽  
Germplasm Collection ◽  
Sequence Repeat ◽  
Simple Sequence Repeat Markers ◽  
Reference Collection ◽  
Simple Sequence

A study was conducted to identify genotypes present in a Moroccan fig germplasm collection and provide the first database for a reference collection in northern Morocco. In total, 75 fig samples were analyzed using 8 intersimple sequence repeat primers and 6 simple sequence repeat loci. From these samples, we identified 72 fig genotypes. In genetically heterogeneous cultivars, genotypes under the same denomination were distinguished by both molecular markers and pomological traits. Molecular analysis was used to classify the germplasm into 46 well-defined cultivars and 6 caprifig trees. The remaining genotypes were not clearly identified due to three cases of mislabeling and four cases of homonymy. No evidence was found for the occurrence of geographically widespread genotypes.

scholarly journals Molecular Characterization of 5α-Reductase Type 2 Deficiency and Fertility in a Swedish Family1

1998 ◽  
Vol 83 (9) ◽  
pp. 3236-3238 ◽  
Author(s):  
Agneta Nordenskjöld ◽  
Sten-Anders Ivarsson
Keyword(s):  
Molecular Characterization ◽  
Molecular Analysis ◽  
External Genitalia ◽  
Hypospadias Repair ◽  
Swedish Family ◽  
Exon 4 ◽  
Compound Heterozygotes ◽  
5Α Reductase Deficiency

The molecular background of 5α-reductase type 2 deficiency was investigated in a Swedish family with no known consanguinity and in which the affected males were fertile. The three male siblings were born with ambiguous external genitalia, and the diagnosis of 5α-reductase deficiency was established at the ages of 16, 14, and 10 yr, respectively. All three siblings underwent surgery for hypospadias repair. At least two of the brothers are demonstrably fertile. Molecular analysis showed the three brothers to be compound heterozygotes, carrying two different mutations in exon 4 of the 5α-reductase type 2 gene. The two mutations (G196S and H231R) have been described previously and reported to give rise to partially functioning enzymes, which may explain the milder phenotype and perhaps the fertility in the preset three patients.

scholarly journals Phenotypic and Molecular Characterization of Mango Cultivars in Southwest Nigeria

2022 ◽  
Vol 22 (1) ◽  
pp. 151-159
Author(s):  
Olawale Arogundade ◽  
Joshua Olumide Matthew ◽  
Omolara Ifeoluwa Akinyoola ◽  
Pamela Eloho Akin-Idowu ◽  
Sunday Oluseyi Solomom Akinyemi
Keyword(s):  
Molecular Characterization ◽  
Southwest Nigeria

scholarly journals Molecular approach of the fragile chromosomal region Xq31-34 in cattle (Bos taurus) by microdissection and DOP-PCR

2008 ◽  
Vol 60 (4) ◽  
pp. 926-931 ◽  
Author(s):  
S. Llambí ◽  
M.V. Arruga
Keyword(s):  
Molecular Characterization ◽  
Molecular Analysis ◽  
Chromosomal Region ◽  
Bos Taurus ◽  
Fragile Sites ◽  
Molecular Approach ◽  
Chromosomal Fragility ◽  
Chromosomal Microdissection

Fragile sites (FS) are chromosomal regions where the normal compactation of chromatine is not observed. FRAXA (Fra Xq27.3, X sexual chromosome) is one of the most studied FS in humans. FRAXA is an expansion of the trinucleotide CGG located in the gene FMR-1. In cattle, sites of chromosomal fragility were reported in BTAX, associated with different pathologies and fertility impairment. Chromosomal microdissection has became a valuable tool for isolating chromatine fragments. In this work, it was combined the chromosomal microdissection technique with DOP-PCR in order to carry out a molecular analysis of the fragile chromosomal region BTAXq31-34. In that region, polymorphic DNA-RAPD sequences (GC rich) are present and sequences of the gene FMR-1 are missing. The results showed the usefulness of the microdissection-DOP-PCR technique for molecular characterization of fragile chromosomal sites in cattle.

scholarly journals A new species, Dactylosoma piperis n. sp. (Apicomplexa, Dactylosomatidae), from the pepper frog Leptodactylus labyrinthicus (Anura, Leptodactylidae) from Mato Grosso State, Brazil.

Parasite ◽  
2020 ◽  
Vol 27 ◽  
pp. 73
Author(s):  
Letícia Pereira Úngari ◽  
Edward Charles Netherlands ◽  
André Luiz Quagliatto Santos ◽  
Edna Paulino de Alcantara ◽  
Enzo Emmerich ◽  
...  
Keyword(s):  
New Species ◽  
Molecular Characterization ◽  
Molecular Analysis ◽  
Peripheral Blood ◽  
18S Rdna ◽  
Mato Grosso ◽  
Lower Vertebrates ◽  
A New Species ◽  
Partial 18S

The Dactylosomatidae Jakowska and Negrelli, 1955 are one of four families belonging to adeleorinid coccidia and comprise the genera Babesiosoma Jakowska and Nigrelli, 1956 and Dactylosoma Labbé, 1894. These blood protozoa occur in peripheral blood of lower vertebrates, and are commonly reported parasitising amphibians. The present study describes Dactylosoma piperis n. sp. from the pepper frog Leptodactylus labyrinthicus (Spix, 1824) (Anura: Leptodactylidae), collected in 2018 at the municipality of Araguaiana, Mato Grosso State, Brazil, based on morphology of intra-erythrocytic trophozoite, primary and secondary merogonic stages and a molecular analysis (partial 18S rDNA). Dactylosoma piperis n. sp. forms a well-supported clade with other Dactylosomatidae. This is the first molecular characterization of a species of Dactylosoma from a Brazilian anuran.

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