scholarly journals Communication from learned to innate olfactory processing centers is required for memory retrieval in Drosophila

2017 ◽  
Author(s):  
Michael-John Dolan ◽  
Ghislain Belliart-Guérin ◽  
Alexander Shakeel Bates ◽  
Yoshinori Aso ◽  
Shahar Frechter ◽  
...  
Keyword(s):  
Behavioral Response ◽  
Memory Retrieval ◽  
Mushroom Body ◽  
Behavioral Responses ◽  
Cell Types ◽  
Olfactory Behavior ◽  
Olfactory Neurons ◽  
Appropriate Behavior ◽  
Processing Centre ◽  
Olfactory Processing

AbstractAnimals can show either learned or innate behavioral responses to a given stimulus. How these circuits interact to produce an appropriate behavioral response is unknown. In the Drosophila olfactory system, the lateral horn (LH) and the mushroom body (MB) are thought to mediate innate and learned olfactory behavior respectively, although the function of the LH has not been directly tested. Here we identify two LH cell-types (PD2a1/b1) that receive input from an MB output neuron required for recall of aversive olfactory memories. In contrast to the model above we find that PD2a1/b1 are required for aversive memory retrieval. PD2a1/b1 activity is modulated by training, indicating that memory information is passed to the innate olfactory processing centre. We map the connectivity of PD2a1/b1 to other olfactory neurons with connectomic data. This provides a circuit mechanism by which learned and unlearned olfactory information can interact to produce appropriate behavior.

scholarly journals Neurogenetic dissection of the Drosophila lateral horn reveals major outputs, diverse behavioural functions, and interactions with the mushroom body

eLife ◽  
2019 ◽  
Vol 8 ◽  
Author(s):  
Michael-John Dolan ◽  
Shahar Frechter ◽  
Alexander Shakeel Bates ◽  
Chuntao Dan ◽  
Paavo Huoviala ◽  
...  
Keyword(s):  
Mushroom Body ◽  
Cell Types ◽  
Olfactory Cues ◽  
Body Site ◽  
Olfactory Behavior ◽  
Gal4 Driver ◽  
Sensory Stimuli ◽  
Lateral Horn ◽  
Functional Understanding ◽  
Innate Behaviour

Animals exhibit innate behaviours to a variety of sensory stimuli including olfactory cues. In Drosophila, one higher olfactory centre, the lateral horn (LH), is implicated in innate behaviour. However, our structural and functional understanding of the LH is scant, in large part due to a lack of sparse neurogenetic tools for this region. We generate a collection of split-GAL4 driver lines providing genetic access to 82 LH cell types. We use these to create an anatomical and neurotransmitter map of the LH and link this to EM connectomics data. We find ~30% of LH projections converge with outputs from the mushroom body, site of olfactory learning and memory. Using optogenetic activation, we identify LH cell types that drive changes in valence behavior or specific locomotor programs. In summary, we have generated a resource for manipulating and mapping LH neurons, providing new insights into the circuit basis of innate and learned olfactory behavior.

scholarly journals Internal state configures olfactory behavior and early sensory processing in Drosophila larvae

2021 ◽  
Vol 7 (1) ◽  
pp. eabd6900
Author(s):  
Katrin Vogt ◽  
David M. Zimmerman ◽  
Matthias Schlichting ◽  
Luis Hernandez-Nunez ◽  
Shanshan Qin ◽  
...  
Keyword(s):  
Food Deprivation ◽  
Sensory Processing ◽  
Antennal Lobe ◽  
Internal State ◽  
Behavioral Responses ◽  
Projection Neuron ◽  
Projection Neurons ◽  
Olfactory Behavior ◽  
Appropriate Behavior ◽  
State Dependent

Animals exhibit different behavioral responses to the same sensory cue depending on their internal state at a given moment. How and where in the brain are sensory inputs combined with state information to select an appropriate behavior? Here, we investigate how food deprivation affects olfactory behavior in Drosophila larvae. We find that certain odors repel well-fed animals but attract food-deprived animals and that feeding state flexibly alters neural processing in the first olfactory center, the antennal lobe. Hunger differentially modulates two output pathways required for opposing behavioral responses. Upon food deprivation, attraction-mediating uniglomerular projection neurons show elevated odor-evoked activity, whereas an aversion-mediating multiglomerular projection neuron receives odor-evoked inhibition. The switch between these two pathways is regulated by the lone serotonergic neuron in the antennal lobe, CSD. Our findings demonstrate how flexible behaviors can arise from state-dependent circuit dynamics in an early sensory processing center.

scholarly journals Functional and Anatomical Specificity in a Higher Olfactory Centre

2018 ◽  
Author(s):  
Shahar Frechter ◽  
Alexander S. Bates ◽  
Sina Tootoonian ◽  
Michael-John Dolan ◽  
James D. Manton ◽  
...  
Keyword(s):  
Sensory Processing ◽  
Mushroom Body ◽  
Cell Types ◽  
Projection Neurons ◽  
Olfactory Behavior ◽  
Population Code ◽  
Odor Coding ◽  
Sensory Stimuli ◽  
Anatomical Analysis ◽  
Sparse Population

AbstractMost sensory systems are organized into parallel neuronal pathways that process distinct aspects of incoming stimuli. For example, in insects, second order olfactory projection neurons target both the mushroom body, which is required for learning, and the lateral horn (LH), which has been proposed to mediate innate olfactory behavior. Mushroom body neurons encode odors in a sparse population code, which does not appear stereotyped across animals. In contrast the functional principles of odor coding in the LH remain poorly understood. We have carried out a comprehensive anatomical analysis of the Drosophila LH, counting ~1400 neurons; combining genetic driver lines, anatomical and functional criteria, we identify 165 LHN cell types. We then show that genetically labeled LHNs have stereotyped odor responses across animals for 33 of these cell types. LHN tuning can be ultra-sparse (1/40 odors tested), but on average single LHNs respond to three times more odors than single projection neurons. This difference can be rationalized by our observation that LHNs are better odor categorizers, likely due to pooling of input projection neurons responding to different odors of the same category. Our results reveal some of the principles by which a higher sensory processing area can extract innate behavioral significance from sensory stimuli.

scholarly journals Functional and anatomical specificity in a higher olfactory centre

eLife ◽  
2019 ◽  
Vol 8 ◽  
Author(s):  
Shahar Frechter ◽  
Alexander Shakeel Bates ◽  
Sina Tootoonian ◽  
Michael-John Dolan ◽  
James Manton ◽  
...  
Keyword(s):  
Olfactory System ◽  
Mushroom Body ◽  
Cell Types ◽  
Sensory Systems ◽  
Projection Neurons ◽  
Olfactory Behavior ◽  
Population Code ◽  
Odor Coding ◽  
Sensory Stimuli ◽  
Lateral Horn

Most sensory systems are organized into parallel neuronal pathways that process distinct aspects of incoming stimuli. In the insect olfactory system, second order projection neurons target both the mushroom body, required for learning, and the lateral horn (LH), proposed to mediate innate olfactory behavior. Mushroom body neurons form a sparse olfactory population code, which is not stereotyped across animals. In contrast, odor coding in the LH remains poorly understood. We combine genetic driver lines, anatomical and functional criteria to show that the Drosophila LH has ~1400 neurons and >165 cell types. Genetically labeled LHNs have stereotyped odor responses across animals and on average respond to three times more odors than single projection neurons. LHNs are better odor categorizers than projection neurons, likely due to stereotyped pooling of related inputs. Our results reveal some of the principles by which a higher processing area can extract innate behavioral significance from sensory stimuli.

scholarly journals Olfactory processing in the lateral horn of Drosophila

2021 ◽  
Vol 383 (1) ◽  
pp. 113-123
Author(s):  
Sudeshna Das Chakraborty ◽  
Silke Sachse
Keyword(s):  
Functional Properties ◽  
Olfactory System ◽  
Cell Types ◽  
Specific Cell ◽  
Olfactory Behavior ◽  
Lateral Horn ◽  
Behavioral Decision ◽  
Animal Phyla ◽  
Survival And Reproduction ◽  
Almost All

AbstractSensing olfactory signals in the environment represents a crucial and significant task of sensory systems in almost all organisms to facilitate survival and reproduction. Notably, the olfactory system of diverse animal phyla shares astonishingly many fundamental principles with regard to anatomical and functional properties. Binding of odor ligands by chemosensory receptors present in the olfactory peripheral organs leads to a neuronal activity that is conveyed to first and higher-order brain centers leading to a subsequent odor-guided behavioral decision. One of the key centers for integrating and processing innate olfactory behavior is the lateral horn (LH) of the protocerebrum in insects. In recent years the LH of Drosophila has garnered increasing attention and many studies have been dedicated to elucidate its circuitry. In this review we will summarize the recent advances in mapping and characterizing LH-specific cell types, their functional properties with respect to odor tuning, their neurotransmitter profiles, their connectivity to pre-synaptic and post-synaptic partner neurons as well as their impact for olfactory behavior as known so far.

Using Notches to Uncover Optimization Frictions and Structural Elasticities: Theory and Evidence from Pakistan *

2013 ◽  
Vol 128 (2) ◽  
pp. 669-723 ◽  
Author(s):  
Henrik J. Kleven ◽  
Mazhar Waseem
Keyword(s):  
Administrative Data ◽  
Behavioral Response ◽  
Behavioral Responses ◽  
Tax Incentives ◽  
Missing Mass ◽  
Zero Mass ◽  
Long Run ◽  
Choice Sets ◽  
Price Distortions

Abstract We develop a framework for nonparametrically identifying optimization frictions and structural elasticities using notches—discontinuities in the choice sets of agents—introduced by tax and transfer policies. Notches create excess bunching on the low-tax side and missing mass on the high-tax side of a cutoff, and they are often associated with a region of strictly dominated choice that would have zero mass in a frictionless world. By combining excess bunching (observed response attenuated by frictions) with missing mass in the dominated region (frictions), it is possible to uncover the structural elasticity that would govern behavior in the absence of frictions and arguably capture long-run behavior. We apply our framework to tax notches in Pakistan using rich administrative data. While observed bunching is large and sharp, optimization frictions are also very large as the majority of taxpayers in dominated ranges are unresponsive to tax incentives. The combination of large observed bunching and large frictions implies that the frictionless behavioral response to notches is extremely large, but the underlying structural elasticity driving this response is nevertheless modest. This highlights the inefficiency of notches: by creating extremely strong price distortions, they induce large behavioral responses even when structural elasticities are small.

Invasion of Personal Space as a Function of Age, Sex, and Race

1976 ◽  
Vol 38 (3) ◽  
pp. 959-965 ◽  
Author(s):  
Larry M. Dean ◽  
Frank N. Willis ◽  
James M. La Rocco
Keyword(s):  
Motor Activity ◽  
Exploratory Behavior ◽  
Behavioral Response ◽  
Behavioral Responses ◽  
Personal Space ◽  
Male And Female ◽  
Black And White

Reactions to the invasion of personal space in terms of age, sex, and race of the invaders were investigated. Children, grouped by sex (male and female), race (black and white), and age (5, 8, and 10 yr. old), invaded the personal space of 192 adults grouped by sex (male and female) and race (black and white). The six types of behavioral responses were: avoidance, aggression, exploratory behavior, facilitative behavior, excess motor activity, and failure to respond. Responses to personal space invasion were not affected by sex. Blacks responded more often than whites but did not differ with regard to any particular type of behavioral response. Age of the invader had a significant effect on type of response given by adults whose personal space was invaded. It was concluded that the age of the invader was much more important than race or sex in determining the response to invasion of personal space.

scholarly journals Psychological Distress, Early Behavioral Response, and Perception Toward the COVID-19 Pandemic Among Health Care Workers in North Shoa Zone, Oromiya Region

2021 ◽  
Vol 12 ◽  
Author(s):  
Kemal Jemal ◽  
Berhanu Senbeta Deriba ◽  
Tinsae Abeya Geleta
Keyword(s):  
Health Care ◽  
Psychological Distress ◽  
Health Care Workers ◽  
Behavioral Response ◽  
Behavioral Responses ◽  
Health Care Facilities ◽  
Medical Laboratory ◽  
Event Scale ◽  
Care Workers ◽  
Medical Laboratory Professionals

Background: The coronavirus disease 2019 (COVID-19) pandemic has had a significant psychological impact on health care workers (HCWs). Therefore, this study inspects the mental health status, behavioral response, and perception among HCWs (nurses, physicians, and medical laboratory workers) during the COVID-19 pandemic in public health care facilities.Methods: A facilities-based cross-sectional study was conducted in July 2020. A simple random sampling technique was used to select study participants. Data were collected by self-report administered questionnaires using Patient Health Questionnaire-9 (PHQ-9) for depression, General Anxiety Disorder-7 (GAD-7) for anxiety, Insomnia Severity Index (ISI) for insomnia, Impact of Event Scale-Revised (IES-R) for psychological distress, Perceived Threat Scale for perception, and Behavioral Response Inquiry for the behavioral response. Moreover, bivariable and multivariable logistic regressions analysis was used to identify the association between dependent and independent variables at p-value <0.05.Results: A total of 417 (98.6%) HCWs responded to a self-administered questionnaire. The proportion of HCWs who had moderate to severe symptoms of psychological distress, depression, anxiety, and insomnia during the COVID-19 pandemic were 58, 16.3, 30.7, and 15.9%, respectively. Three-fifth of the nurses, medical laboratory professionals (62.2%), and physicians (59.2%) had reported good behavioral responses toward the COVID-19 pandemic. More than three-fifths of the nurses had reported poor perception toward the COVID-19 pandemic. Conversely, 61.2% of physicians and three-fourths (75.5%) of medical laboratory professionals had reported good perception toward the COVID-19 pandemic. Female and married participants, those working in the emergency unit, those with poor behavioral responses, and those with poor perception toward the COVID-19 pandemic were significantly associated with symptoms of psychological distress, depression, anxiety, and insomnia.Conclusions: Psychological impacts among physicians, nurses, and medical laboratory professionals are high during the COVID-19 pandemic. The Ethiopian Federal Ministry of Health should aim to protect all HCWs' psychological well-being during the COVID-19 pandemic with appropriate interventions and accurate information response.

scholarly journals Testing the habituation assumption underlying models of parasitoid foraging behavior

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e3097 ◽  
Author(s):  
Paul K. Abram ◽  
Antonino Cusumano ◽  
Katrina Abram ◽  
Stefano Colazza ◽  
Ezio Peri
Keyword(s):  
Foraging Behavior ◽  
Time Allocation ◽  
Behavioral Response ◽  
Behavioral Responses ◽  
Protein Synthesis Inhibitor ◽  
Partial Recovery ◽  
Behavioral Strategies ◽  
Atpase Inhibitor ◽  
Wide Range ◽  
Training Interval

BackgroundHabituation, a form of non-associative learning, has several well-defined characteristics that apply to a wide range of physiological and behavioral responses in many organisms. In classic patch time allocation models, habituation is considered to be a major mechanistic component of parasitoid behavioral strategies. However, parasitoid behavioral responses to host cues have not previously been tested for the known, specific characteristics of habituation.MethodsIn the laboratory, we tested whether the foraging behavior of the egg parasitoidTrissolcus basalisshows specific characteristics of habituation in response to consecutive encounters with patches of host (Nezara viridula) chemical contact cues (footprints), in particular: (i) a training interval-dependent decline in response intensity, and (ii) a training interval-dependent recovery of the response.ResultsAs would be expected of a habituated response, wasps trained at higher frequencies decreased their behavioral response to host footprints more quickly and to a greater degree than those trained at low frequencies, and subsequently showed a more rapid, although partial, recovery of their behavioral response to host footprints. This putative habituation learning could not be blocked by cold anesthesia, ingestion of an ATPase inhibitor, or ingestion of a protein synthesis inhibitor.DiscussionOur study provides support for the assumption that diminishing responses of parasitoids to chemical indicators of host presence constitutes habituation as opposed to sensory fatigue, and provides a preliminary basis for exploring the underlying mechanisms.

The Drosophila mushroom body is a quadruple structure of clonal units each of which contains a virtually identical set of neurones and glial cells

Development ◽  
1997 ◽  
Vol 124 (4) ◽  
pp. 761-771 ◽  
Author(s):  
K. Ito ◽  
W. Awano ◽  
K. Suzuki ◽  
Y. Hiromi ◽  
D. Yamamoto
Keyword(s):  
Glial Cells ◽  
Sensory Integration ◽  
Mushroom Body ◽  
Expression Patterns ◽  
Cell Lineage ◽  
Cell Types ◽  
Enhancer Trap ◽  
Adult Brain ◽  
A New Technique ◽  
Lineage Analysis

The mushroom body (MB) is an important centre for higher order sensory integration and learning in insects. To analyse the development and organisation of the MB neuropile in Drosophila, we performed cell lineage analysis in the adult brain with a new technique that combines the Flippase (flp)/FRT system and the GAL4/UAS system. We showed that the four mushroom body neuroblasts (MBNbs) give birth exclusively to the neurones and glial cells of the MB, and that each of the four MBNb clones contributes to the entire MB structure. The expression patterns of 19 GAL4 enhancer-trap strains that mark various subsets of MB cells revealed overlapping cell types in all four of the MBNb lineages. Partial ablation of MBNbs using hydroxyurea showed that each of the four neuroblasts autonomously generates the entire repertoire of the known MB substructures.

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