scholarly journals Production of Multilayer Cell Mass from Olfactory Stem Cell and Its Utilization Potential in Nervous Tissue Regeneration

2017 ◽  
Author(s):  
Olga N. Oztel ◽  
Adil Allahverdiyev ◽  
Aysegul Batioglu Karaaltin ◽  
Melahat Bağırova ◽  
Ercument Ovali
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Nerve Cell ◽  
Tissue Regeneration ◽  
Cell Mass ◽  
Experimental Studies ◽  
Nerve Tissue ◽  
Supporting Material ◽  
Olfactory Tissue

AbstractBackgroundExperimental studies performed with human olfactory nerve stem cells haveshown that these cells can ameliorate nerve cell regeneration. Developing a method of repairing nerve damage solely using stem cells without the need of any supporting material is important.MethodsA multilayer cell mass was obtained from olfactory tissue-derived mesenchymal stem cells with high viability and proliferation capability using a protocol devoid of scaffolds or any other artificial supporting material. First, human olfactory tissue-derived mesenchymal stem cells were isolated, cultured, and characterized. Next, consecutive passages were conducted to obtain multilayer cell growth. The resulting cell mass could be suitable for tissue engineering models as well as nerve cell or tissue regeneration studies in the future.ResultsViability and adhesive properties of the resulting cell mass were examined and found to be suitable for use in nerve tissue regeneration.ConclusionIt is suggested that an in vitro-produced olfactory stem cell mass can be applied to a very small damaged region and could have a high potential for microenvironment formation.

Amniotic Stem Cell-Conditioned Media for the Treatment of Nerve and Muscle Pathology: A Systematic Review

2021 ◽  
Author(s):  
Chukwuweike Gwam ◽  
Ahmed Emara ◽  
Nequesha Mohamed ◽  
Noor Chughtai ◽  
Johannes Plate ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Tissue Regeneration ◽  
Cell Damage ◽  
Conditioned Media ◽  
Nerve Tissue ◽  
Muscle Pathology ◽  
Loss Of Function ◽  
Cell Based Therapy

Muscle and nerve tissue damage can elicit a significant loss of function and poses as a burden for patients and healthcare providers. Even for tissues, such as the peripheral nerve and skeletal muscle, that harbor significant regenerative capacity, innate regenerative processes often lead to less than optimal recovery and residual loss of function. The reasons for poor regeneration include significant cell damage secondary to oxidative stress, poor recruitment of resident stem cells, and an unfavorable microenvironment for tissue regeneration. Stem cell-based therapy was once thought as a potential therapy in tissue regeneration, due to its self-renewal and multipotent capabilities. Early advocates for cellular-based therapy pointed to the pluripotent nature of stem cells, thus eluding to its ability to differentiate into resident cells as the source of its regenerative capability. However, increasing evidence has revealed a lack of engraftment and differentiation of stem cells, thereby pointing to stem cell paracrine activity as being responsible for its regenerative potential. Stem cell-conditioned media houses biomolecular factors that portray significant regenerative potential. Amniotic-derived stem cell-conditioned media (AFS-CM) has been of particular interest because of its ease of allocation and in vitro culture. The purpose of this review is to report the results of studies that assess the role of AFS-CM for nerve and muscle conditions. In this review, we will cover the effects of AFS-CM on cellular pathways, genes, and protein expression for different nerve and muscle cell types.

scholarly journals Immunomodulatory Functions of Mesenchymal Stem Cells in Tissue Engineering

2019 ◽  
Vol 2019 ◽  
pp. 1-18 ◽  
Author(s):  
Haojiang Li ◽  
Shi Shen ◽  
Haitao Fu ◽  
Zhenyong Wang ◽  
Xu Li ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Tissue Regeneration ◽  
Tissue Damage ◽  
Material Selection ◽  
Immune Microenvironment ◽  
Chronic Injury ◽  
Research Fields

The inflammatory response to chronic injury affects tissue regeneration and has become an important factor influencing the prognosis of patients. In previous stem cell treatments, it was revealed that stem cells not only have the ability for direct differentiation or regeneration in chronic tissue damage but also have a regulatory effect on the immune microenvironment. Stem cells can regulate the immune microenvironment during tissue repair and provide a good “soil” for tissue regeneration. In the current study, the regulation of immune cells by mesenchymal stem cells (MSCs) in the local tissue microenvironment and the tissue damage repair mechanisms are revealed. The application of the concepts of “seed” and “soil” has opened up new research avenues for regenerative medicine. Tissue engineering (TE) technology has been used in multiple tissues and organs using its biomimetic and cellular cell abilities, and scaffolds are now seen as an important part of building seed cell microenvironments. The effect of tissue engineering techniques on stem cell immune regulation is related to the shape and structure of the scaffold, the preinflammatory microenvironment constructed by the implanted scaffold, and the material selection of the scaffold. In the application of scaffold, stem cell technology has important applications in cartilage, bone, heart, and liver and other research fields. In this review, we separately explore the mechanism of MSCs in different tissue and organs through immunoregulation for tissue regeneration and MSC combined with 3D scaffolds to promote MSC immunoregulation to repair damaged tissues.

Dendrimer surface orientation of the RGD peptide affects mesenchymal stem cell adhesion

RSC Advances ◽  
2016 ◽  
Vol 6 (55) ◽  
pp. 49839-49844 ◽  
Author(s):  
Y. Vida ◽  
D. Collado ◽  
F. Najera ◽  
S. Claros ◽  
J. Becerra ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Cell Adhesion ◽  
Mesenchymal Stem Cell ◽  
Tissue Regeneration ◽  
Surface Orientation ◽  
Rgd Peptide

Mesenchymal stem cells (MSCs) are promising candidates for a range of tissue regeneration applications.

scholarly journals The Effect of Diabetes Mellitus on IGF Axis and Stem Cell Mediated Regeneration of the Periodontium

2021 ◽  
Vol 8 (12) ◽  
pp. 202
Author(s):  
Nancy M. S. Hussein ◽  
Josie L. Meade ◽  
Hemant Pandit ◽  
Elena Jones ◽  
Reem El-Gendy
Keyword(s):  
Diabetes Mellitus ◽  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Tissue Regeneration ◽  
Diabetic Patients ◽  
Periodontal Tissue ◽  
Signalling Molecules ◽  
Periodontal Tissues

Periodontitis and diabetes mellitus (DM) are two of the most common and challenging health problems worldwide and they affect each other mutually and adversely. Current periodontal therapies have unpredictable outcome in diabetic patients. Periodontal tissue engineering is a challenging but promising approach that aims at restoring periodontal tissues using one or all of the following: stem cells, signalling molecules and scaffolds. Mesenchymal stem cells (MSCs) and insulin-like growth factor (IGF) represent ideal examples of stem cells and signalling molecules. This review outlines the most recent updates in characterizing MSCs isolated from diabetics to fully understand why diabetics are more prone to periodontitis that theoretically reflect the impaired regenerative capabilities of their native stem cells. This characterisation is of utmost importance to enhance autologous stem cells based tissue regeneration in diabetic patients using both MSCs and members of IGF axis.

scholarly journals Mesenchymal Stem Cells in Bone Tissue Regeneration and Application to Bone Healing

2009 ◽  
Vol 78 (4) ◽  
pp. 635-642 ◽  
Author(s):  
Michal Crha ◽  
Alois Nečas ◽  
Robert Srnec ◽  
Jan Janovec ◽  
Ladislav Stehlík ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Bone Healing ◽  
Experimental Studies ◽  
Bone Defects ◽  
Bone Tissue Regeneration ◽  
Bone Lesions ◽  
Tumour Resection

This synoptic study gives a concise overview of current knowledge of bone healing, the role of mesenchymal stem cells in bone tissue regeneration and contemporary possibilities of supporting regeneration of damaged bone. Attention of research concerning the healing of fractures with extensive loss of bone tissue following trauma, the treatment of belatedly healing or non-healing fractures or the healing of segmental bone defects following tumour resection, is focused on development of three-dimensional scaffolds planted with mesenchymal stem cells that might be used for reconstruction of such large bone lesions. Presented are possibilities of transplantation of mesenchymal stem cells combined with biomaterials into bone defects, including the results of our own experimental studies dealing with the use of stem cells in the treatment of damaged tissues of the musculoskeletal system in animal models.

scholarly journals Mesenchymal stem cells in the dental tissues: perspectives for tissue regeneration

2011 ◽  
Vol 22 (2) ◽  
pp. 91-98 ◽  
Author(s):  
Carlos Estrela ◽  
Ana Helena Gonçalves de Alencar ◽  
Gregory Thomas Kitten ◽  
Eneida Franco Vencio ◽  
Elisandra Gava
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Tissue Regeneration ◽  
Stem Cell Research ◽  
Cell Biology ◽  
Dental Tissues ◽  
New Findings ◽  
Dental Stem Cell

In recent years, stem cell research has grown exponentially owing to the recognition that stem cell-based therapies have the potential to improve the life of patients with conditions that range from Alzheimer’s disease to cardiac ischemia and regenerative medicine, like bone or tooth loss. Based on their ability to rescue and/or repair injured tissue and partially restore organ function, multiple types of stem/progenitor cells have been speculated. Growing evidence demonstrates that stem cells are primarily found in niches and that certain tissues contain more stem cells than others. Among these tissues, the dental tissues are considered a rich source of mesenchymal stem cells that are suitable for tissue engineering applications. It is known that these stem cells have the potential to differentiate into several cell types, including odontoblasts, neural progenitors, osteoblasts, chondrocytes, and adipocytes. In dentistry, stem cell biology and tissue engineering are of great interest since may provide an innovative for generation of clinical material and/or tissue regeneration. Mesenchymal stem cells were demonstrated in dental tissues, including dental pulp, periodontal ligament, dental papilla, and dental follicle. These stem cells can be isolated and grown under defined tissue culture conditions, and are potential cells for use in tissue engineering, including, dental tissue, nerves and bone regeneration. More recently, another source of stem cell has been successfully generated from human somatic cells into a pluripotent stage, the induced pluripotent stem cells (iPS cells), allowing creation of patient- and disease-specific stem cells. Collectively, the multipotency, high proliferation rates, and accessibility make the dental stem cell an attractive source of mesenchymal stem cells for tissue regeneration. This review describes new findings in the field of dental stem cell research and on their potential use in the tissue regeneration.

The effect of PEGylated hollow gold nanoparticles on stem cell migration: potential application in tissue regeneration

Nanoscale ◽  
2017 ◽  
Vol 9 (28) ◽  
pp. 9848-9858 ◽  
Author(s):  
Maria del Mar Encabo-Berzosa ◽  
Maria Sancho-Albero ◽  
Alejandra Crespo ◽  
Vanesa Andreu ◽  
Victor Sebastian ◽  
...  
Keyword(s):  
Stem Cells ◽  
Gold Nanoparticles ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Tissue Regeneration ◽  
Cell Types ◽  
Stem Cell Migration ◽  
Hollow Gold Nanoparticles ◽  
Migration Potential ◽  
Different Cell Types

Mesenchymal stem cells (MSCs) not only can be differentiated into different cell types but also have tropism towards injured or inflamed tissues serving as repair cells.

scholarly journals A mathematical model of cartilage regeneration after chondrocyte and stem cell implantation – I: the effects of growth factors

2019 ◽  
Vol 10 ◽  
pp. 204173141982779
Author(s):  
Kelly Campbell ◽  
Shailesh Naire ◽  
Jan Herman Kuiper
Keyword(s):  
Mathematical Model ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Growth Factors ◽  
Healing Process ◽  
Experimental Studies ◽  
Cartilage Regeneration ◽  
Long Term Results ◽  
Cell Based Therapy

Autologous chondrocyte implantation is a cell-based therapy for treating chondral defects. The procedure begins by inserting chondrocytes into the defect region. The chondrocytes initiate healing by proliferating and depositing extracellular matrix, which allows them to migrate into the defect until it is completely filled with new cartilage. Mesenchymal stem cells can be used instead of chondrocytes with similar long-term results. The main differences are at early times since mesenchymal stem cells must first differentiate into chondrocytes before cartilage is formed. To better understand this repair process, we present a mathematical model of cartilage regeneration after cell therapy. We extend our previous work to include the cell–cell interaction between mesenchymal stem cells and chondrocytes via growth factors. Our results show that matrix formation is enhanced at early times in the presence of growth factors. This study reinforces the importance of mesenchymal stem cell and chondrocyte interaction in the cartilage healing process as hypothesised in experimental studies.

scholarly journals Effects of Lipids and Lipoproteins on Mesenchymal Stem Cells Used in Cardiac Tissue Regeneration

2020 ◽  
Vol 21 (13) ◽  
pp. 4770 ◽  
Author(s):  
Yi-Hsiung Lin ◽  
Lin Kang ◽  
Wen-Han Feng ◽  
Tsung-Lin Cheng ◽  
Wei-Chung Tsai ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Regenerative Medicine ◽  
Tissue Regeneration ◽  
Blood Lipids ◽  
Cardiac Tissue ◽  
Lipid Lowering ◽  
Lipids And Lipoproteins ◽  
Msc Differentiation

Mesenchymal stem cells (MSCs) have two characteristics of interest for this paper: the ability to self-renew, and the potential for multiple-lineage differentiation into various cells. MSCs have been used in cardiac tissue regeneration for over a decade. Adult cardiac tissue regeneration ability is quite low; it cannot repair itself after injury, as the heart cells are replaced by fibroblasts and lose function. It is therefore important to search for a feasible way to repair and restore heart function through stem cell therapy. Stem cells can differentiate and provide a source of progenitor cells for cardiomyocytes, endothelial cells, and supporting cells. Studies have shown that the concentrations of blood lipids and lipoproteins affect cardiovascular diseases, such as atherosclerosis, hypertension, and obesity. Furthermore, the MSC lipid profiles, such as the triglyceride and cholesterol content, have been revealed by lipidomics, as well as their correlation with MSC differentiation. Abnormal blood lipids can cause serious damage to internal organs, especially heart tissue. In the past decade, the accumulated literature has indicated that lipids/lipoproteins affect stem cell behavior and biological functions, including their multiple lineage capability, and in turn affect the outcome of regenerative medicine. This review will focus on the effect of lipids/lipoproteins on MSC cardiac regenerative medicine, as well as the effect of lipid-lowering drugs in promoting cardiomyogenesis-associated MSC differentiation.

scholarly journals Biomaterials and Stem Cells in the Treatment of Articular Cartilage, Meniscal, Physeal, Bone, Ligamentous and Tendineous Defects

2008 ◽  
Vol 77 (2) ◽  
pp. 277-284 ◽  
Author(s):  
A. Nečas ◽  
L. Plánka ◽  
R. Srnec ◽  
P. Raušer ◽  
L. Urbanová ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Articular Cartilage ◽  
Stem Cell Transplantation ◽  
Cell Transplantation ◽  
Musculoskeletal System ◽  
Experimental Studies

This review briefly summarizes existing results and main trends in experimental studies dealing with the possibilities of the use of biomaterials and mesenchymal stem cells in the treatment of diseases affecting tissues derived from the mesoderm, i.e. articular cartilage, meniscus, physis, bone, ligaments and tendons. The aim of these experimental studies is to find optimal forms of treatment of the diseases of individual parts of the musculoskeletal system using methods of tissue engineering with the use of stem cell transplantation.

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