scholarly journals Host-induced gene silencing compromises Verticillium wilt in tomato and Arabidopsis

2016 ◽  
Author(s):  
Yin Song ◽  
Bart P.H.J. Thomma
Keyword(s):  
Gene Silencing ◽  
Verticillium Wilt ◽  
Host Plants ◽  
Plant Protection ◽  
Tobacco Rattle Virus ◽  
Wilt Disease ◽  
Tomato Plants ◽  
Wide Range ◽  
Soil Borne Fungi ◽  
Important Disease

SUMMARYVerticillium wilt, caused by soil-borne fungi of the genus Verticillium, is an economically important disease that affects a wide range of host plants. Unfortunately, host resistance against Verticillium wilts is not available for many plant species, and the disease is notoriously difficult to combat. Host-induced gene silencing (HIGS) is an RNA interference (RNAi) based process in which small RNAs are produced by the host plant to target parasite transcripts. HIGS has emerged as a promising strategy for improving plant resistance against pathogens by silencing genes that are essential for these pathogens. Here, we assessed whether HIGS can be utilized to suppress Verticillium wilt disease by silencing previously identified virulence genes of V. dahliae through the host plants tomato and Arabidopsis. In transient assays, tomato plants were agroinfiltrated with Tobacco rattle virus (TRV) constructs to target V. dahliae transcripts. Subsequent V. dahliae inoculation revealed suppression of Verticillium wilt disease in some, but not all, cases. Next, expression of RNAi constructs targeting V. dahliae transcripts was pursued in stable transgenic Arabidopsis thaliana plants. Also in this host, V. dahliae inoculation revealed reduced Verticillium wilt disease in some cases. Thus, our study suggests that, depending on the target gene chosen, HIGS against V. dahliae is operational in tomato and A. thaliana plants and may act as a plant protection approach that may be used in Verticillium wilt-susceptible crops.

Effect of Olive Mill Wastewater of in the Control of Olive Verticillium Wilt Caused by Verticillium dahlia Kleb

2021 ◽  
Vol 39 (4) ◽  
pp. 273-280
Author(s):  
Khiyam Muhrez ◽  
◽  
Mohamad Zakaria Tawil ◽  
Bassema Barhoum ◽  
◽  
...  
Keyword(s):  
Disease Severity ◽  
Verticillium Wilt ◽  
Plant Protection ◽  
Wilt Disease ◽  
Olive Mill Wastewater ◽  
Waste Waters ◽  
Verticillium Dahlia ◽  
Olive Trees ◽  
Mill Waste ◽  
Olive Mill

Muhrez, K.A., M.Z. Tawil and B. Barhoum. 2021. Effect of Olive Mill Wastewater of in the Control of Olive Verticillium Wilt Caused by Verticillium dahlia Kleb. Arab Journal of Plant Protection, 39(4): 273-280. https://doi.org/10.22268/AJPP-39.4.273280 This research aimed to evaluate the application of olive mill wastewater in controlling Verticillium dahliae the causal agent of olive wilt disease on olive trees. This study was carried out on 2 years old olive local variety (Khderi) plants planted in pots after it has been artificial inoculated with V. dahliae, and severity was recorded within 90 days. Olive mill wastewater (omww) efficacy was estimated at the end of the experiment. Severity was increased from 8.89% before treatment to 28.89% 60 days after treatment, and decreased to 13.33% 90 days after treatment with omww 450 ml/l. Disease severity increased from 11.11% before treatment to 37.78% 60 days after treatment and decreased to 35.56% 90 days after treatment with omww 300 ml/l. Whereas, disease severity increased from 17.78% before treatment to 40% 90 days after treatment with azoxystrobin compared to the inoculated control where disease severity increased from 13.33% before treatment to 100% 90 days after treatment. Omww 450 ml/l had the highest efficacy of 80% followed by azoxystrobin 70%, and omww 300 ml/l 57.33%. It can be concluded that olive mill wastewater has a potential application as a bio-fungicide for the control of olive verticillium wilt disease. Keywords: Olive mill waste waters, Verticillium dahlia, olive, Fungicide.

scholarly journals Development of resistant crop plants to parasitic weeds based on trans-specific gene silencing

2013 ◽  
Author(s):  
Radi Aly ◽  
John I. Yoder
Keyword(s):  
Gene Silencing ◽  
Host Plants ◽  
Target Genes ◽  
Tobacco Rattle Virus ◽  
Genetic Resistance ◽  
Transgenic Tomato ◽  
Specific Gene ◽  
Virus Induced Gene Silencing ◽  
Parasitic Weeds ◽  
Experimental Systems

Broomrapes (Orobanche/Phelipanchespp.) are holo parasitic plants that subsist on the roots of a variety of agricultural crops and cause severe losses to the yield quality and quantity. Effective methods for controlling parasitic weeds are scarce, with only a few known cases of genetic resistance. In the current study, we proposed an improved strategy for the control of parasitic weeds based on trans-specific gene-silencing of three parasite genes at once. We used two strategies to express dsRNA containing selected sequences of three Phelipancheaegyptiacagenes PaACS, PaM6PR and PaPrx1 (pma): transient expression using Tobacco rattle virus (TRV:pma) as a virus-induced gene-silencing (VIGS) vector and stable expression in transgenic tomato Solanumlycopersicum(Mill.) plants harboring a hairpin construct (pBINPLUS35:pma). siRNA-mediated transgene-silencing (20–24 nt) was detected in the host plants. Our results demonstrate that the quantities of PaACSand PaM6PR transcripts from P. aegyptiacatubercles grown on transgenic tomato or on Tobacco rattle virus-infected Nicotianabenthamianaplants were significantly reduced. However, only partial reductions in the quantity of PaPrx1 transcripts were observed in the parasite tubercles grown on tomato and on N. benthamianaplants. Concomitant with the suppression of the target genes, there were significant decreases in the number and weight of the parasite tubercles that grew on the host plants, in both the transient and the stable experimental systems. The results of the work carried out using both strategies point to the movement of mobile exogenous siRNA from the host to the parasite, leading to the impaired expression of essential parasite target genes. In light of the importance of parasitic weeds to world agriculture and the difficulty of obtaining resistance by conventional methods, we assume that genetic resistance based on the silencing of key metabolic genes in the parasite is now feasible. BARD Report - Project4622 Page 2 of 60 

scholarly journals Eutetranychus orientalis Klein (oriental spider mite). Phytosanitary risk analysis for Ukraine

2020 ◽  
pp. 1-4
Author(s):  
L. Titova ◽  
Yu. Klechkovskyi ◽  
O. Palahina
Keyword(s):  
Risk Analysis ◽  
Host Plants ◽  
Spider Mite ◽  
Plant Protection ◽  
Reproductive Potential ◽  
Climatic Conditions ◽  
Scientific Publications ◽  
Air Masses ◽  
Wide Range ◽  
The World

Goal. To carry out the analysis of phytosanitary risk for Ukraine of a dangerous quarantine pest Eutetranychus orientalis Klein (oriental spider mite). Research Methods. The main method is information-analytical. We conducted analytical research and analysis of reports from the Mediterranean, World Plant Protection Organizations, literary sources of scientific publications and online resources. Phytosanitary risk analysis (PRA) was performed according to the EPPO standards PM 5/3 (5), PM 5/1, PM 5/4 [3, 4, 5]. The possibility of acclimatization of the pest was determined using modern computer programs IDRISI SELVA, MapInfo Pro 15.0 and AgroAtlas. Results. Eutetranychus orientalis Klein (oriental spider mite) absent in Ukraine is a polyphage, it can damage 217 species of plants, preferring citrus, and is widespread in the world. Many species of plants that are host plants of the eastern spider mite grow in Ukraine and are important in the production of fruits, vegetables, and oils. The primary pest habitat was the Middle East, but currently E. orientalis is found in many countries in Asia, Africa, Europe, Oceania and is in a wide climatic range. Thus, in European countries which lie in the pest habitat, the climate is subtropical, Mediterranean and temperate, transitional to continental (Greece, Spain, Cyprus, Turkey). In Asia (China, India, Israel, Iraq, Saudi Arabia), the area of the pest occupies territories dominated by tropical, subtropical and temperate climates. In recent years, several species of tetrachnid mites, including E. orientalis, have expanded their geographical range, mainly due to increased trade and travel around the world, posing a threat to agriculture in many countries. Under optimal conditions, 25 generations per year can occur. The spread of E. orientalis is by air masses or anthropic. Distribution of E. orientalis is carried out by air masses or anthropically. The most likely pathway for spreading the pest is through infected planting material. Given the high reproductive potential of E. Orientalis, the rate of expansion of the habitat, the diversity of the plant’s food supply, its adaptability to a wide range of climatic conditions, there is a need to analyze the phytosanitary risk (AFR) of the eastern spider mite for Ukraine. The end result of the research is the determination of the quarantine status of the pest and the proposal for amendments to the «List of regulated pests» that are quarantined in Ukraine. Conclusions. There is a high likelihood of acclimatization of Eutetranychus orientalis in Ukraine, which is due to the large number of host plants and compliance with the species requirements to the climatic conditions. Potential habitat area in Ukraine may be the southern coast of Crimea. Phytosanitary risk analysis of Eutetranychus orientalis Klein (Eastern spider mite) for Ukraine identified the need for the pest to be granted the status of a quarantine organism absent in Ukraine (list A1) and to amend the «List of regulated pests of Ukraine».

RESISTANCE TO VERTICILLIUM WILT IN F1 GENERATIONS OF SELF-FERTILIZED SPECIES OF FRAGARIA

1958 ◽  
Vol 36 (3) ◽  
pp. 297-299 ◽  
Author(s):  
W. Newton ◽  
M. C. J. van Adrichem
Keyword(s):  
Verticillium Wilt ◽  
Wilt Disease ◽  
Fragaria Chiloensis

The F1 generation of selfed plants of Fragaria chiloensis, F. ovalis, and F. yukonensis contained seedlings resistant to the verticillium wilt disease. Selfed F. orientalis plants yielded seedlings that carried considerable tolerance but selfed F. vesca, F. bracteata, and F. virginiana plants yielded neither tolerant nor resistant seedlings. Asexually propagated plants of the seven species were all susceptible to the disease.

Optimisation of tobacco rattle virus-induced gene silencing in Arabidopsis

2006 ◽  
Vol 33 (4) ◽  
pp. 347 ◽  
Author(s):  
Changchun Wang ◽  
Xinzhong Cai ◽  
Xuemin Wang ◽  
Zhong Zheng
Keyword(s):  
Gene Silencing ◽  
High Throughput ◽  
Function Analysis ◽  
Tobacco Rattle Virus ◽  
Phytoene Desaturase ◽  
Growth Stages ◽  
Virus Induced Gene Silencing ◽  
Gene Functions ◽  
Genes Encoding ◽  
Gene Function Analysis

Arabidopsis thaliana (L.) Heynh. is a model plant species in which to study plant gene functions. Recently developed virus-induced gene silencing (VIGS) offers a rapid and high-throughput technique platform for gene function analysis. In this paper we report optimisation of tobacco rattle virus (TRV)-induced gene silencing in Arabidopsis. The parameters potentially affecting the efficiency of VIGS in Arabidopsis were investigated. These included the concentration and pre-incubation of Agrobacterium inocula (agro-inocula), the concentration of acetosyringone included in agro-inocula, the Agrobacterium inoculation (agro-inoculation) method, the ecotypes and the growth stages of Arabidopsis plants for agro-inoculation, and the growth temperature of agro-inoculated plants. The optimised VIGS procedure involves preparing the agro-inocula with OD600 of 2.0, pre-incubating for 2 h in infiltration buffer containing 200 μm acetosyringone, agro-inoculating by vacuum infiltration, and growth of agro-inoculated plants at 22 −24°C. Following this procedure consistent and highly efficient VIGS was achieved for the genes encoding phytoene desaturase (PDS) and actin in Arabidopsis. The silencing phenotype lasts for at least 6 weeks, and is applicable in at least seven ecotypes, including Col-0, Cvi-0, Sd, Nd-1, Ws-0, Bay-0 and Ler. TRV-induced VIGS was expressed not only in leaves, but also in stems, inflorescences and siliques. However, VIGS was not transmissible through seed to the subsequent generation. The optimised procedure of the TRV-induced gene silencing should facilitate high-throughput functional analysis of genes in Arabidopsis.

Ustilago hypodytes. [Descriptions of Fungi and Bacteria].

1984 ◽  
Author(s):  
J. E. M. Mordue
Keyword(s):  
New Zealand ◽  
North America ◽  
South America ◽  
Root System ◽  
Geographical Distribution ◽  
Host Plants ◽  
Meristematic Tissue ◽  
Wide Range ◽  
Vegetative Multiplication ◽  
Established Infection

Abstract A description is provided for Ustilago hypodytes. Information is included on the disease caused by the organism, its transmission, geographical distribution, and hosts. HOSTS: A wide range of grasses, including species of Agropyron (many), Ammophila, Brachypodium, Bromus, Calamagrostis, Diplachne, Distichlis, Elymus (many), Festuca, Glyceria, Hilaria, Hordeum, Haynaldia, Lygeum, Melica, Orysopsis, Panicum, Phalaris, Phleum, Poa (many), Puccinellia, Secale, Sitanion, Sporobolus, Stipa (many), and Trisetum. DISEASE: Stem smut of grasses. GEOGRAPHICAL DISTRIBUTION: Chiefly a temperate species found in Europe (including Denmark, Finland, France, Germany, Hungary, Italy, Romania, Sweden, Switzerland, UK, USSR, Yugoslavia) and North America (Canada, USA) and extending to central and South America (Argentina, Peru, Uruguay), N. Africa (Libya, Morocco, Tunisia), Japan, Australia and New Zealand. TRANSMISSION: Not fully understood, though inoculation experiments have demonstrated that infection occurs in mature vegetative plants (possibly through meristematic tissue), not seeds or flowers (22, 240; 24, 511). Once established, infection is systemic, probably overwintering in the root system and spreading by vegetative multiplication of host plants as well as from plant to plant (24, 511; 19, 720).

scholarly journals Applications of Photocatalytic Disinfection

2010 ◽  
Vol 2010 ◽  
pp. 1-11 ◽  
Author(s):  
Joanne Gamage ◽  
Zisheng Zhang
Keyword(s):  
Indoor Air ◽  
Food Industry ◽  
Plant Protection ◽  
Water Disinfection ◽  
Photocatalytic Disinfection ◽  
Wide Range ◽  
Test Organisms ◽  
Drinking Water Disinfection ◽  
End Use ◽  
Biological And Medical Applications

Due to the superior ability of photocatalysis to inactivate a wide range of harmful microorganisms, it is being examined as a viable alternative to traditional disinfection methods such as chlorination, which can produce harmful byproducts. Photocatalysis is a versatile and effective process that can be adapted for use in many applications for disinfection in both air and water matrices. Additionally, photocatalytic surfaces are being developed and tested for use in the context of “self-disinfecting” materials. Studies on the photocatalytic technique for disinfection demonstrate this process to have potential for widespread applications in indoor air and environmental health, biological, and medical applications, laboratory and hospital applications, pharmaceutical and food industry, plant protection applications, wastewater and effluents treatment, and drinking water disinfection. Studies on photocatalytic disinfection using a variety of techniques and test organisms are reviewed, with an emphasis on the end-use application of developed technologies and methods.

scholarly journals Onorchard habitats for Nysius huttoni an important kiwifruit passenger pest

2014 ◽  
Vol 67 ◽  
pp. 326-326
Author(s):  
C.A. Rowe ◽  
M.G. Hill ◽  
D.P. Logan
Keyword(s):  
Native Species ◽  
Host Plants ◽  
Ground Vegetation ◽  
Preliminary Survey ◽  
Weed Species ◽  
Quarantine Pest ◽  
Polygonum Aviculare ◽  
Brassica Crops ◽  
Wide Range ◽  
High Incidence

Nysius huttoni is a native species of wheat bug which is an occasional quarantine pest on export kiwifruit Wheat bug has a wide range of host plants including wheat and brassica crops as well as many weed species The kiwifruit harvest of 2013 had a high incidence of wheat bug finds on fruit in packhouses In response an orchard survey was undertaken to identify orchard habitats used by wheat bug Twentyfive orchards where wheat bug was found during packing in 2013 were surveyed from the Te Puke area from February to March At each orchard four habitats were searched (1) ground vegetation under the kiwifruit canopy (2) the loadout zone (3) edge of the loadout zone and (4) grassland surrounding the was found in and around the loadout zone associated with weed species notably Polygonum aviculare the dominant weed species in loadout zones No wheat bug was found under the kiwifruit canopy and the remaining 4 were found in grassland This preliminary survey suggests that the risk of wheat bug infestation can be reduced considerably by keeping loadout zones free of weeds during spring and summer

scholarly journals Trehalose induces tomato defenses and increases drought tolerance and bacterial wilt disease resistance

2021 ◽  
Author(s):  
April M MacIntyre ◽  
Valerian Meline ◽  
Zachary Gorman ◽  
Steven P Augustine ◽  
Carolyn J Dye ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Stomatal Conductance ◽  
Water Use ◽  
Bacterial Wilt ◽  
Xylem Sap ◽  
Wilt Disease ◽  
Tomato Plants ◽  
Bacterial Wilt Disease ◽  
Infected Tomato ◽  
Use Efficiency

Ralstonia solanacearum causes plant bacterial wilt disease, leading to severe crop losses. Xylem sap from R. solanacearum-infected tomato is enriched in host produced trehalose. Water stressed plants accumulate the disaccharide trehalose, which increases drought tolerance via abscisic acid (ABA) signaling networks. Because infected plants have reduced water flow, we hypothesized that bacterial wilt physiologically mimics drought stress, which trehalose could mitigate. Transcriptomic responses of susceptible vs. resistant tomato plants to R. solanacearum infection revealed differential expression of drought-associated genes, including those involved in ABA and trehalose metabolism. ABA was enriched in xylem sap from R. solanacearum-infected plants. Treating roots with ABA lowered stomatal conductance and reduced R. solanacearum stem colonization. Treating roots with trehalose increased ABA in xylem sap and reduced plant water use by reducing stomatal conductance and temporarily improving water use efficiency. Further, trehalose-treated plants were more resistant to bacterial wilt disease. Trehalose treatment also upregulated expression of salicylic acid (SA)-dependent defense genes, increased xylem sap levels of SA and other antimicrobial compounds, and increased wilt resistance of SA-insensitive NahG tomato plants. Additionally, trehalose treatment increased xylem concentrations of jasmonic acid and related oxylipins. Together, these data show that exogenous trehalose reduced both water stress and bacterial wilt disease and triggered systemic resistance. This suite of responses revealed unexpected linkages between plant responses to biotic and abiotic stress and suggests that that R. solanacearum-infected tomato plants produce more trehalose to improve water use efficiency and increase wilt disease resistance. In turn, R. solanacearum degrades trehalose as a counter-defense.

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