scholarly journals Biosynthesis of the Antibiotic Nonribosomal Peptide Penicillin in Baker’s Yeast

2016 ◽  
Author(s):  
Ali R. Awan ◽  
Benjamin A. Blount ◽  
David J. Bell ◽  
Jack C. H. Ho ◽  
Robert M. McKiernan ◽  
...  
Keyword(s):  
Baker’S Yeast ◽  
Growth Media ◽  
Subcellular Localisation ◽  
Peptide Antibiotics ◽  
Baker's Yeast ◽  
Beta Lactam ◽  
Nonribosomal Peptide ◽  
Rational Engineering ◽  
Long Read ◽  
Efficient Expression

AbstractFungi are a valuable source of enzymatic diversity and therapeutic natural products including antibiotics. By taking genes from a filamentous fungus and directing their efficient expression and subcellular localisation, we here engineer the baker’s yeast Saccharomyces cerevisiae to produce and secrete the antibiotic penicillin, a beta-lactam nonribosomal peptide. Using synthetic biology tools combined with long-read DNA sequencing, we optimise productivity by 50-fold to produce bioactive yields that allow spent S. cerevisiae growth media to have antibacterial action against Streptococcus bacteria. This work demonstrates that S. cerevisiae can be engineered to perform the complex biosynthesis of multicellular fungi, opening up the possibility of using yeast to accelerate rational engineering of nonribosomal peptide antibiotics.

scholarly journals Biosynthesis of the antibiotic nonribosomal peptide penicillin in baker’s yeast

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Ali R. Awan ◽  
Benjamin A. Blount ◽  
David J. Bell ◽  
William M. Shaw ◽  
Jack C.H. Ho ◽  
...  
Keyword(s):  
Baker’S Yeast ◽  
Baker's Yeast ◽  
Nonribosomal Peptide

scholarly journals Yeast Species, Strains, and Growth Media Mediate Attraction of Drosophila suzukii (Diptera: Drosophilidae)

Insects ◽  
2019 ◽  
Vol 10 (8) ◽  
pp. 228 ◽  
Author(s):  
Rodrigo Lasa ◽  
Laura Navarro-de-la-Fuente ◽  
Anne C. Gschaedler-Mathis ◽  
Manuel R. Kirchmayr ◽  
Trevor Williams
Keyword(s):  
Yeast Species ◽  
Baker’S Yeast ◽  
Invasive Pest ◽  
Growth Media ◽  
Baker's Yeast ◽  
Drosophila Suzukii ◽  
Corn Syrup ◽  
Control Techniques ◽  
Baited Traps ◽  
Males And Females

Specific ecological interactions between insects and microbes have potential in the development of targeted pest monitoring or control techniques for the spotted wing drosophilid, Drosophila suzukii (Matsumura), an exotic invasive pest of soft fruit. To evaluate D. suzukii attraction to yeast species from preferred types of fruit, three yeasts were isolated from blackberry fruit and two yeasts from raspberry fruit and used to bait simple plastic bottle traps. Saccharomyces cerevisiae and Hanseniaspora uvarum were identified from blackberries, whereas a different H. uvarum strain was identified from raspberry. Yeast identification was based on sequence analysis of the D1/D2 domain of the large subunit 26S rRNA gene. Commercial baker’s yeast (S. cerevisiae) was similar or more effective for the capture of D. suzukii males and females than yeasts isolated from blackberry or raspberry when grown in sucrose. However, when grown in corn syrup, a strain of S. cerevisiae from blackberry captured the highest number of females and a strain of H. uvarum isolated from raspberry captured high numbers of males and females. Species of Candida, Hanseniaspora, and Pichia from a laboratory yeast collection did not outperform baker’s yeast in pairwise tests when grown in sucrose solution or yeast-peptone-dextrose medium. The raspberry strain of H. uvarum grown in corn syrup outperformed S. cerevisiae grown in sucrose, in terms of captures in baited traps under laboratory conditions. We conclude that yeast species, strain, and growth medium can have a marked influence on D. suzukii attraction to baited traps, a finding that could assist in the development of yeast-related monitoring or control techniques targeted at this pest.

Valorization of residual soft drinks by baker's yeast production and insight for dairy wastewater whey incorporation

2019 ◽  
Vol 79 (4) ◽  
pp. 635-644
Author(s):  
Mariam Kasmi ◽  
Amjad Kallel ◽  
Lobna Elleuch ◽  
Moktar Hamdi ◽  
Ismail Trabelsi
Keyword(s):  
Growth Medium ◽  
Soft Drink ◽  
Soft Drinks ◽  
Baker’S Yeast ◽  
Dairy Wastewater ◽  
Partial Substitution ◽  
Growth Media ◽  
Baker's Yeast ◽  
Conversion Rates ◽  
Yeast Production

Abstract Residuals are responsible for the polluting load increase of soft drink industry wastewater due to their high sugar contents. The present work proposes an upstream segregation of residuals to be biologically treated by the bioconversion of their carbohydrates content into baker's yeast biomass. Carbonated soft drinks (CSD) and nectars and juices (NJ) ranges were considered. Different incorporation ratios of NJ in the CSD (0–75%) have been investigated for balanced growth medium. Despite the nitrogen deficiency of media, results showed that NJ incorporation promoted the microbial growth. Media containing more than 50% of NJ exhibited ∼25% sugar-biomass conversion rates. The chemical oxygen demand (COD) of the media exceeded 70% at the end of fermentation. Moreover, valuable components were recovered by yeast production. Nutrient consumption rates varied from 65.4% for sugar and calcium content to in excess of 99% for protein and other minerals. In order to investigate an available and low-cost source of nitrogen for yeast production, partial substitution of the soft drink growth medium by bactofugate whey was evaluated. The soft drink-whey mixture medium fermentation resulted in 63% COD removal rate after 28 h. Meanwhile, the biomass production yield revealed an improvement of about 25% compared to the balanced soft drink medium (NJ50).

Baker’s yeast-catalyzed synthesis of optically pure 4-tert-butyl-3-hydroxy beta-lactam cis-(3R,4S) and trans-(3R,4R) diastereomers

2002 ◽  
Vol 80 (7) ◽  
pp. 796-800 ◽  
Author(s):  
Margaret M Kayser ◽  
Yan Yang ◽  
Marko D Mihovilovic ◽  
Anton Feicht ◽  
Fernande D Rochon
Keyword(s):  
Baker’S Yeast ◽  
Yeast Cells ◽  
Side Chain ◽  
Baker's Yeast ◽  
Beta Lactam ◽  
Yeast Saccharomyces Cerevisiae ◽  
Enantiomerically Pure ◽  
Cis And Trans Isomers ◽  
Cis And Trans ◽  
Optically Pure

Baker's yeast (Saccharomyces cerevisiae) reductions were applied to the synthesis of the paclitaxel C-13 side-chain analogue. An easily synthesized alpha-keto-beta-lactam (1-(4-methoxyphenyl)-4-tert-butylazetidin-2,3-dione, 4) was reduced by yeast cells to give a mixture of enantiomerically pure cis and trans isomers. Both compounds were isolated and characterized, and their absolute configurations were confirmed by X-ray crystallographic analysis.Key words: Taxol®, C-13 side-chain, paclitaxel analogues, optically pure alpha-hydroxy-beta-lactams, baker's yeast-catalyzed reductions.

COD REDUCTION OF BAKER'S YEAST WASTEWATER USING BATCH ELECTROCOAGULATION

2014 ◽  
Vol 13 (12) ◽  
pp. 3153-3160 ◽  
Author(s):  
Zakaria Al-Qodah ◽  
Mohammad Al-Shannag ◽  
Kholoud Alananbeh ◽  
Nahla Bouqellah ◽  
Eman Assirey ◽  
...  
Keyword(s):  
Baker’S Yeast ◽  
Baker's Yeast ◽  
Cod Reduction ◽  
Yeast Wastewater

The use of β-glucan extracted from baker’s yeast (Saccharomyces cerevisiae) to increase non-specific immune system and resistence of tilapia (Oreochromis niloticus) to Aeromonas hydrophila

2013 ◽  
pp. 92
Author(s):  
Ida N Jamal ◽  
Reiny A Tumbol ◽  
Remy E.P Mangindaan
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Immune System ◽  
Aeromonas Hydrophila ◽  
Phagocytic Activity ◽  
Baker’S Yeast ◽  
Baker's Yeast ◽  
Yeast Saccharomyces Cerevisiae ◽  
Randomized Design ◽  
The Difference ◽  
Motile Aeromonas Septicaemia

Motile Aeromonas Septicaemia disease (MAS) attacking tilapia has increased in recent years as a consequence of intensive aquaculture activities, which led to losses in aquaculture industry. The agent causing MAS disease is Aeromonas hydrophila. The disease can be controlled with the β-glucan. As immunostimulants, β-glucans can also increase resistance in farmed tilapia. Studies on the use of β-glucan extracted from baker's yeast Saccharomyces cerevisiae was intended to evaluate the non-specific immune system of tilapia that were challenged with Aeromonas hydrophila. The method used was an experimental method with a completely randomized design consisting of four treatments with three replicats. The dose of β-glucan used as treatments were 0 mg.kg-1 fish (Control), 5 mg.kg-1 fish (B), 10 mg.kg-1 fish (C) and 20 mg.kg-1 fish (D), each treatment as injected three times at intervals of 3 days, the injection volume of 0.5 ml/fish for nine days and resistance surveillance for seven days. The results showed that the difference in the amount of β-glucan and the frequency of the injected real influence on total leukocytes, phagocytic activity and resistance. Total leukocytes, phagocytic activity and resistance to treatment was best achieved by the administration of C a dose of  10 mg.kg-1 of the fish© Penyakit Motil Aeromonas Septicaemia (MAS) yang menyerang ikan nila mengalami peningkatan selama beberapa tahun terakhir sebagai konsekuensi dari kegiatan akuakultur intensif, yang menyebabkan kerugian dalam industri budidaya. Agen utama penyebab penyakit MAS adalah Aeromonas hydrophila. Untuk mengendalikan penyakit tersebut dapat dilakukan dengan pemberian β-glukan. Sebagai imunostimulan, β-glukan juga dapat  meningkatkan resistensi pada ikan nila yang dibudidayakan. Pengkajian mengenai pemanfaatan β-glukan yang diekstrak dari ragi roti Saccharomyces cerevisiae dimaksudkan untuk menguji sistem imun non spesifik ikan nila yang diuji tantang dengan bakteri Aeromonas hydrophila. Metode yang digunakan yaitu metode eksperimen dengan rancangan acak lengkap yang terdiri dari empat perlakuan dan tiga ulangan. Dosis β-glukan  yang digunakan sebagai perlakuan sebesar 0 mg.kg-1 ikan (Kontrol), 5 mg.kg-1 ikan (B), 10 mg.kg-1 ikan (C) dan 20 mg.kg-1 ikan (D), masing-masing perlakuan diinjeksi sebanyak 3 kali dengan interval waktu 3 hari selama 9 hari, volume injeksi 0,5 mL/ekor ikan dan pengamatan resistensi selama tujuh hari. Hasil penelitian menunjukkan perbedaan jumlah β-glukan dan frekuensi pemberian yang diinjeksikan memberikan pengaruh nyata terhadap total leukosit, aktivitas fagositosis dan resistensi. Total leukosit, aktivitas fagositosis dan resistensi terbaik dicapai pada perlakuan C dengan dosis 10 mg.kg-1 ikan©

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