scholarly journals Long-term monitoring of inflammation in the mammalian gut using programmable commensal bacteria

2016 ◽  
Author(s):  
David T Riglar ◽  
Michael Baym ◽  
S Jordan Kerns ◽  
Matthew J Niederhuber ◽  
Roderick T Bronson ◽  
...  
Keyword(s):  
Mouse Model ◽  
Cost Effective ◽  
Oxygen Species ◽  
Genetic Circuits ◽  
E Coli ◽  
Inflammatory Bowel ◽  
Engineered Bacteria ◽  
Term Monitoring

AbstractInflammation in the gut, caused by infection and autoimmunity, remains challenging to effectively detect, monitor, and treat. Here, we engineer a commensal mouse E. coli strain to record exposure to tetrathionate, a downstream product of reactive oxygen species generated during inflammation. Using these programmed bacteria to sense in situ levels we show that tetrathionate accompanies inflammation during Salmonella-induced colitis in mice and is elevated in an inflammatory bowel disease mouse model. We demonstrate long-term genetic stability and associated robust function of synthetic genetic circuits in bacteria colonizing the mammalian gut. These results demonstrate the potential for engineered bacteria to stably and reliably probe pathophysiological processes for which traditional diagnostics may not be feasible or cost-effective.One sentence summaryEngineered bacteria record an inflammatory response in an IBD mouse model and are genetically stable during long-term growth in the mouse gut.

scholarly journals ENVIRONMENTAL POLLUTION BY WASTEWATER FROM BROWN COAL PROCESSING ¬ A REMEDIATION CASE STUDY IN GERMANY

2014 ◽  
Vol 22 (1) ◽  
pp. 71-83 ◽  
Author(s):  
Arndt Wiessner ◽  
Jochen A. Müller ◽  
Peter Kuschk ◽  
Uwe Kappelmeyer ◽  
Matthias Kästner ◽  
...  
Keyword(s):  
Environmental Pollution ◽  
Large Scale ◽  
Pilot Scale ◽  
Wastewater Disposal ◽  
Interdisciplinary Approaches ◽  
Phenolic Wastewater ◽  
Term Monitoring

The large scale of the contamination by the former carbo-chemical industry in Germany requires new and often interdisciplinary approaches for performing an economically sustainable remediation. For example, a highly toxic and dark-colored phenolic wastewater from a lignite pyrolysis factory was filled into a former open-cast pit, forming a large wastewater disposal pond. This caused an extensive environmental pollution, calling for an ecologically and economically acceptable strategy for remediation. Laboratory-scale investigations and pilot-scale tests were carried out. The result was the development of a strategy for an implementation of full-scale enhanced in situ natural attenuation on the basis of separate habitats in a meromictic pond. Long-term monitoring of the chemical and biological dynamics of the pond demonstrates the metamorphosis of a former highly polluted industrial waste deposition into a nature-integrated ecosystem with reduced danger for the environment, and confirmed the strategy for the chosen remediation management.

scholarly journals Mouse model of ulcerative colitis using trinitrobenzene sulfonic acid

2015 ◽  
Vol 10 (4) ◽  
pp. 860
Author(s):  
Irfan Ahmad Rather ◽  
Vivek K. Bajpai ◽  
Nam Gyeong-Jun
Keyword(s):  
Ulcerative Colitis ◽  
Inflammatory Bowel Disease ◽  
Mouse Model ◽  
Bowel Disease ◽  
Sulfonic Acid ◽  
Intestinal Inflammation ◽  
Cost Effective ◽  
Trinitrobenzene Sulfonic Acid ◽  
Clinical Presentations ◽  
Inflammatory Bowel

<p>Animal model of intestinal inflammation is of paramount significance that aids in discerning the pathologies underlying ulcerative colitis and Crohn’s disease, the two clinical presentations of inflammatory bowel disease. The 2,4,6-trinitrobenzene sulfonic acid (TNBS) colitis model represents one such intestinal inflammation-prototype that is generated in susceptible strains of mice through intra-rectal instillation of compound TNBS. In this paper, we demonstrate the experimental induction of TNBS-mediated colitis in a susceptible strain of ICR mice. This can be done by the following steps: a) acclimation, b) induction and c) observation. TNBS-mouse model provides the information in shortest possible time and simultaneously represents a cost effective and highly reproducible model method of studying the pathogenesis of inflammatory bowel disease.</p><p><strong>Video Clips</strong></p><p><a href="https://youtube.com/v/6MsuIGzH3uA">Acclimation and induction of TNBS</a>:          4.5 min</p><p><a href="https://youtube.com/v/ya66SNwoVag">Observation and drug administration</a>:      1.5 min</p>

Development and Assembly of a MEMS Based High-Sensitivity Relative Gravimeter for Multi-Pixel Imaging Applications 

2021 ◽  
Author(s):  
Karl Toland ◽  
Abhinav Prasad ◽  
Andreas Noack ◽  
Kristian Anastasiou ◽  
Richard Middlemiss ◽  
...  
Keyword(s):  
High Sensitivity ◽  
Cost Effective ◽  
Mount Etna ◽  
Earth Tides ◽  
Gravity Measurements ◽  
Stepper Motors ◽  
Long Term Monitoring ◽  
The Stability ◽  
Term Monitoring

&lt;p&gt;The manufacture and production of a&amp;#160;high-sensitivity&amp;#160;cost-effective gravimeter has the potential to change the&amp;#160;methodology&amp;#160;and efficiency&amp;#160;of gravity measurements. Currently,&amp;#160;the&amp;#160;most common&amp;#160;method&amp;#160;to conduct a survey is&amp;#160;by using&amp;#160;a single gravimeter, usually costing tens of thousands of Dollars,&amp;#160;with measurements taken at multiple locations to&amp;#160;obtain the required data.&amp;#160;The availability of a cost-effective gravimeter&amp;#160;however would allow the user to install multiple gravimeters, at the same cost of a single gravimeter,&amp;#160;to increase the efficiency of&amp;#160;surveys and long-term monitoring.&amp;#160;&amp;#160;&lt;/p&gt;&lt;p&gt;&amp;#160;&lt;/p&gt;&lt;p&gt;Since the previous reporting on a low-drift relative MEMS gravimeter for multi-pixel imaging applications (Prasad, A. et al,&amp;#160;EGU2020-18528), significant&amp;#160;progress&amp;#160;has&amp;#160;been made in the&amp;#160;development and assembly of the previously reported system. Field prototypes have been manufactured and undergone significant testing&amp;#160;to investigate the stability and robustness of the system&amp;#160;in preparation for&amp;#160;the&amp;#160;deployment&amp;#160;of multiple devices&amp;#160;as part of the gravity imager on Mount Etna. The device, known as Wee-g, has several key features which makes it an attractive prospect in the field of gravimetry. Examples of these features are that the Wee-g is small and portable with the ability to connect to the device remotely, can be powered through a mains connected power supply, or through portable batteries, weighs under 4kg, has a low power consumption during normal use of 5W, correct for tilt through manual adjustments or remotely through integrated stepper motors with a total tilt correction range of 5 degrees, the ability to read out tilt of the device through an inclinometer for either alignment or long term monitoring and numerous temperature sensors and heater servos to control the temperature of the MEMS to &lt;1mK.&lt;/p&gt;&lt;p&gt;&amp;#160;&lt;/p&gt;&lt;p&gt;This presentation aims to report on the progress that has been achieved&amp;#160;in the&amp;#160;development and&amp;#160;manufacturing of the prototype devices, various testing of the devices under various laboratory conditions (such as the measurements of the Earth tides, and a relative measurement of gravity at various floor levels), as well as additional&amp;#160;applications that are to be explored in 2021.&amp;#160;&lt;/p&gt;

The Need for In-Situ Pipe Support Testing

2004 ◽  
Author(s):  
Gerry May
Keyword(s):  
Creep Damage ◽  
Cost Effective ◽  
Piping System ◽  
Design Load ◽  
Cost Effective Method ◽  
Accelerated Creep ◽  
Constant Support ◽  
Full Operation

Pipe support functionality is critical to the long term life of piping system. Spring supports degrade with time due to flexing in the spring and wear in constant support bearings. It is not unusual to measure constant support hangers with resistance 25% to 50% different than the design load. This leads to excessive sustained pipe stress, pipe sag (or uplift), and in high temperature systems, accelerated creep damage. Supports may also not move properly from shut down to full operation, which can create excessive fatigue stress, failed hanger components, and other piping system damage. In-situ hanger testing has been found to be a reliable and cost effective method to determine the functionality of pipe supports. Results are used as input to set revised recommended loads, and to determine if any hangers need to be replaced. This paper provides examples of the types of problems that are often found in the field, the method to test, and typical resolutions to maximize the pipe life and minimize the risk of failure.

scholarly journals Serial passage of the human probiotic E. coli Nissle 1917 in an insect host leads to changed bacterial phenotypes

2021 ◽  
Author(s):  
Nicolas C. H. Schröder ◽  
Ana Korša ◽  
Haleluya Wami ◽  
Ulrich Dobrindt ◽  
Joachim Kurtz
Keyword(s):  
Medical Application ◽  
Serial Passage ◽  
Intestinal Colonization ◽  
Genetic Changes ◽  
E Coli ◽  
Novel Host ◽  
Multiple Cycles ◽  
Inflammatory Bowel ◽  
Underlying Mechanisms

Probiotics are living microorganisms that are increasingly and successfully used for the therapy of various diseases. The most common use of probiotics is the therapeutic and preventive application for gastrointestinal disorders. The probiotic Escherichia coli strain Nissle 1917 (EcN) has been proven to effectively prevent and alleviate intestinal diseases, including various types of inflammatory bowel disease. Despite the widespread medical application of EcN, the underlying mechanisms of its protective effect remain elusive. The present work aimed to establish an insect model system to enable further research on the modes of action of EcN and the dynamics of adaptation to a novel host organism. Using a long-term serial passage approach, we orally introduced EcN to the host, the red flour beetle Tribolium castaneum. After multiple cycles of intestinal colonization in beetle larvae, several attributes of the passaged replicate lines were assessed. We observed phenotypic changes in growth and motility but no genetic changes in the lines after passaging through the host and its flour environment. One of the EcN lines exposed to the host displayed peculiar morphological and physiological characteristics showing that serial passage of EcN can generate differential phenotypes.

scholarly journals Phytoindication of environmental mutagenesis in the in-situ conditions using the pollen of selected species of native flora

2021 ◽  
Author(s):  
Karol Mičieta
Keyword(s):  
Pollen Grains ◽  
Heavy Load ◽  
Native Flora ◽  
In Situ Conditions ◽  
Environmental Mutagenesis ◽  
Term Monitoring ◽  
Long Term Experience

The aim of this study is to provide an effective method for indicating ecogenotoxicity in the environment using pollen grains and microspores of selected species of the native flora in the in situ conditions. In the report, we summarize the results of long-term experience with the benefits of native flora species as bioindicators of polluted environments. We present the current results of long-term monitoring of phytoindication of ecogenotoxicity in Bratislava and selected traffic junctions in Slovakia. The increase of pollen grain abortion in the group of localities exposed to a heavy load of traffic pollution demonstrates the ecogenotoxic impact of traffic emissions in the environment. The detailed practical methodological tools and possible difficulties with the classification of abortivity of microspores and pollen grains of these plant species are discussed.

scholarly journals Highly Multiplexed Spatial Mapping of Microbial Communities

2019 ◽  
Author(s):  
Hao Shi ◽  
Warren Zipfel ◽  
Ilana Brito ◽  
Iwijn De Vlaminck
Keyword(s):  
Microbial Communities ◽  
Spatial Organization ◽  
Single Cells ◽  
Cost Effective ◽  
Probe Design ◽  
E Coli ◽  
Rich Diversity ◽  
Effective Technology ◽  
Phylogenetic Resolution

ABSTRACTMapping the complex biogeography of microbial communities in situ with high taxonomic and spatial resolution poses a major challenge because of the high density and rich diversity of species in environmental microbiomes and the limitations of optical imaging technology. Here, we introduce High Phylogenetic Resolution microbiome mapping by Fluorescence In-Situ Hybridization (HiPR-FISH), a versatile and cost-effective technology that uses binary encoding and spectral imaging and machine learning based decoding to create micron-scale maps of the locations and identities of hundreds of microbial species in complex communities. We demonstrate the ability of 10-bit HiPR-FISH to distinguish 1023 E. coli strains, each fluorescently labeled with a unique binary barcode. HiPR-FISH, in conjunction with custom algorithms for automated probe design and segmentation of single-cells in the native context of tissues, reveals the intricate spatial architectures formed by bacteria in the human oral plaque microbiome and disruption of spatial networks in the mouse gut microbiome in response to antibiotic treatment. HiPR-FISH provides a framework for analyzing the spatial organization of microbial communities in tissues and the environment at single cell resolution.

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