scholarly journals A data acquisition and analysis pipeline for scanning angle interference microscopy

2016 ◽  
Author(s):  
Catherine B. Carbone ◽  
Ronald D. Vale ◽  
Nico Stuurman
Keyword(s):  
Data Acquisition ◽  
Open Source ◽  
Open Source Software ◽  
Interference Microscopy ◽  
Analysis Pipeline ◽  
Scanning Angle ◽  
Software And Hardware

AbstractWe describe open source software and hardware tools for calibrating, acquiring, and analyzing images for scanning angle interference microscopy (SAIM) analysis. These tools make it possible for any user with a TIRF microscope equipped with a motorized illumination unit to generate reconstructed images with nanometer precision in the axial (z) direction and diffraction-limited resolution in the lateral (xy) plane.

scholarly journals An acquisition and analysis pipeline for scanning angle interference microscopy

2016 ◽  
Vol 13 (11) ◽  
pp. 897-898 ◽  
Author(s):  
Catherine B Carbone ◽  
Ronald D Vale ◽  
Nico Stuurman
Keyword(s):  
Interference Microscopy ◽  
Analysis Pipeline ◽  
Scanning Angle

scholarly journals High-speed device synchronization in optical microscopy with an open-source hardware control platform

2019 ◽  
Author(s):  
Marshall J. Colville ◽  
Sangwoo Park ◽  
Warren R. Zipfel ◽  
Matthew J. Paszek
Keyword(s):  
Open Source ◽  
High Speed ◽  
Interference Microscopy ◽  
Scanning Angle ◽  
Precise Control ◽  
Synchronization Errors ◽  
Waveform Generation ◽  
High Speeds ◽  
Control And Synchronization ◽  
Open Source Hardware

AbstractRecent advances in fluorescence microscopy have enabled the visualization of subcellular structures at unprecedented resolution. However, the complexity of state-of-the-art microscopes has increased considerably, often requiring the precise control and synchronization of multiple peripheral devices at high speeds. Drawing inspiration from open-source prototyping systems, like the Arduino, we describe the development of a new control platform that adopts the best features of these systems – affordability, facile programmability, and flexible connectivity – but with the scientific-grade inputs and outputs (I/O) and built-in routines that are necessary to control peripherals in advanced microscopy applications. Notably, our platform includes waveform generators and I/O for point-and azimuthal-scanning of excitation in laser-based applications. As a proof of concept, we show how the integration of waveform generation, multiplexed analog outputs, and native hardware triggers into a single central hub provides a versatile platform for performing fast circle-scanning acquisitions, including ring scanning-angle interference microscopy (SAIM), total internal reflection fluorescence (TIRF and ring TIRF) microscopy, and multiangle TIRF (MA-TIRF). We also demonstrate how the low communication latency of our hardware platform can reduce image intensity and reconstruction artifacts arising from synchronization errors produced by software control. Our complete platform, including hardware design files, firmware, API, software, and all associated source code, will be hosted for community-based development and collaboration.

scholarly journals Development and implementation of real-time data acquisition systems for fusion devices with Open Source software

2010 ◽  
Vol 85 (3-4) ◽  
pp. 378-382 ◽  
Author(s):  
M. Zilker ◽  
K. Behler ◽  
T. Bluhm ◽  
P. Heimann ◽  
Ch. Hennig ◽  
...  
Keyword(s):  
Data Acquisition ◽  
Open Source ◽  
Real Time ◽  
Open Source Software ◽  
Time Data ◽  
Data Acquisition Systems ◽  
Real Time Data
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