scholarly journals Sensitive red protein calcium indicators for imaging neural activity

2016 ◽  
Author(s):  
Hod Dana ◽  
Boaz Mohar ◽  
Yi Sun ◽  
Sujatha Narayan ◽  
Andrew Gordus ◽  
...  
Keyword(s):  
Neural Activity ◽  
Emission Spectra ◽  
Tissue Imaging ◽  
C Elegans ◽  
Calcium Indicators ◽  
Large Populations ◽  
Consequent Reduction ◽  
Deep Tissue Imaging ◽  
Genetically Encoded Calcium Indicators

Genetically encoded calcium indicators (GECIs) allow measurement of activity in large populations of neurons and in small neuronal compartments, over times of milliseconds to months. Although GFP-based GECIs are widely used for in vivo neurophysiology, GECIs with red-shifted excitation and emission spectra have advantages for in vivo imaging because of reduced scattering and absorption in tissue, and a consequent reduction in phototoxicity. However, current red GECIs are inferior to the state-of-the-art GFP-based GCaMP6 indicators for detecting and quantifying neural activity. Here we present improved red GECIs based on mRuby (jRCaMP1a, b) and mApple (jRGECO1a), with sensitivity comparable to GCaMP6. We characterized the performance of the new red GECIs in cultured neurons and in mouse, Drosophila, zebrafish and C. elegans in vivo. Red GECIs facilitate deep-tissue imaging, dual-color imaging together with GFP-based reporters, and the use of optogenetics in combination with calcium imaging.

scholarly journals Sensitive red protein calcium indicators for imaging neural activity

eLife ◽  
2016 ◽  
Vol 5 ◽  
Author(s):  
Hod Dana ◽  
Boaz Mohar ◽  
Yi Sun ◽  
Sujatha Narayan ◽  
Andrew Gordus ◽  
...  
Keyword(s):  
Neural Activity ◽  
Emission Spectra ◽  
Tissue Imaging ◽  
C Elegans ◽  
Calcium Indicators ◽  
Large Populations ◽  
Consequent Reduction ◽  
Deep Tissue Imaging ◽  
Genetically Encoded Calcium Indicators

Genetically encoded calcium indicators (GECIs) allow measurement of activity in large populations of neurons and in small neuronal compartments, over times of milliseconds to months. Although GFP-based GECIs are widely used for in vivo neurophysiology, GECIs with red-shifted excitation and emission spectra have advantages for in vivo imaging because of reduced scattering and absorption in tissue, and a consequent reduction in phototoxicity. However, current red GECIs are inferior to the state-of-the-art GFP-based GCaMP6 indicators for detecting and quantifying neural activity. Here we present improved red GECIs based on mRuby (jRCaMP1a, b) and mApple (jRGECO1a), with sensitivity comparable to GCaMP6. We characterized the performance of the new red GECIs in cultured neurons and in mouse, Drosophila, zebrafish and C. elegans in vivo. Red GECIs facilitate deep-tissue imaging, dual-color imaging together with GFP-based reporters, and the use of optogenetics in combination with calcium imaging.

The one-pot synthesis of core/shell/shell CdTe/CdSe/ZnSe quantum dots in aqueous media for in vivo deep tissue imaging

2011 ◽  
Vol 21 (9) ◽  
pp. 2877 ◽  
Author(s):  
Shohei Taniguchi ◽  
Mark Green ◽  
Sarwat B. Rizvi ◽  
Alexander Seifalian
Keyword(s):  
Quantum Dots ◽  
Aqueous Media ◽  
Tissue Imaging ◽  
Core Shell ◽  
Deep Tissue ◽  
One Pot ◽  
One Pot Synthesis ◽  
Deep Tissue Imaging ◽  
The One

scholarly journals Ultrabright red AIEgens for two-photon vascular imaging with high resolution and deep penetration

2018 ◽  
Vol 9 (10) ◽  
pp. 2705-2710 ◽  
Author(s):  
Wei Qin ◽  
Pengfei Zhang ◽  
Hui Li ◽  
Jacky W. Y. Lam ◽  
Yuanjing Cai ◽  
...  
Keyword(s):  
High Resolution ◽  
Vascular Imaging ◽  
Tissue Imaging ◽  
Deep Penetration ◽  
High Contrast ◽  
Deep Tissue ◽  
Two Photon ◽  
High Penetration ◽  
Deep Tissue Imaging

A successful strategy for the design of ultrabright red luminogens with aggregation-induced emission (AIE) features is reported. The AIE dots can be utilized as efficient fluorescent probes for in vivo deep-tissue imaging with high penetration depth and high contrast.

scholarly journals Label-free microendoscopy using a micro-needle imaging probe for in vivo deep tissue imaging

2020 ◽  
Vol 11 (9) ◽  
pp. 4976
Author(s):  
Kwanjun Park ◽  
June Hoan Kim ◽  
Taedong Kong ◽  
Woong Sun ◽  
Jonghwan Lee ◽  
...  
Keyword(s):  
Tissue Imaging ◽  
Label Free ◽  
Deep Tissue ◽  
Imaging Probe ◽  
Deep Tissue Imaging

scholarly journals Imaging of red-shifted photons from bioluminescent tumours using fluorescence by unbound excitation from luminescence

2018 ◽  
Author(s):  
Fabiane Sônego ◽  
Sophie Bouccara ◽  
Thomas Pons ◽  
Nicolas Lequeux ◽  
Anne Danckaert ◽  
...  
Keyword(s):  
Cell Line ◽  
Near Infrared ◽  
Tumour Size ◽  
Visible Spectrum ◽  
Tissue Imaging ◽  
Tumour Detection ◽  
Signal Sensitivity ◽  
Bioluminescence Signal ◽  
Deep Tissue Imaging

AbstractEarly detection of tumours is today a major challenge and requires sensitive imaging methodologies coupled with new efficient probes. Bioluminescence imaging has been widely used in the field of oncology and several cancer cell lines have been genetically modified to provide bioluminescence signals. However, photons that are emitted by the majority of commonly used luciferases are usually in the blue part of the visible spectrum, where tissue absorption is still very high, making deep tissue imaging non-optimal and calling for optimised optical imaging methodologies. We have previously shown that red-shifting of bioluminescence signal by Fluorescence Unbound Excitation from Luminescence (FUEL) is a mean to increase bioluminescence signal sensitivity detection in vivo. Here, we applied FUEL to tumour detection in two different subcutaneous tumour models: the auto-luminescent human embryonic kidney (HEK293) cell line and the murine B16-F10 melanoma cell line previously transfected with the plasmid Luc2. Tumour size and bioluminescence were measured over time and tumour vascularization characterized. We then locally injected near infrared emitting Quantum Dots (NIR QDs)in the tumour site and observed a red-shifting of bioluminescence signal by (FUEL) indicating that FUEL could be used to allow deeper tumour detection.

scholarly journals Optogenetic analysis of Ca++ transients in Caenorhabditis elegans muscle cells during forward and reverse locomotion

2020 ◽  
Author(s):  
Jacob R. Manjarrez ◽  
Magera Shaw ◽  
Roger Mailler
Keyword(s):  
Caenorhabditis Elegans ◽  
Muscle Activation ◽  
Cell Activity ◽  
Model Organism ◽  
Muscle Cells ◽  
Phase Lag ◽  
C Elegans ◽  
Calcium Indicators ◽  
Body Wall Muscles ◽  
Genetically Encoded Calcium Indicators

ABSTRACTUnderstanding how an organism generates movement is an important step toward determining how a system of neurons produces behavior. With only 95 body wall muscles and 302 neurons, Caenorhabditis elegans is an attractive model organism to use in uncovering the connection between neural circuitry and movement. This study provides a comprehensive examination of the muscle cell activity used by C. elegans during both forward and reverse locomotion. By tracking freely moving worms that express genetically encoded calcium indicators in their muscle cells, we directly measure the patterns of activity that occur during movement. We then analyzed these patterns using a variety of signal processing and statistical techniques. Although our results agree with many previous findings, we also discovered there is significantly different mean Ca++ levels in many of the muscle cells during forward and reverse locomotion and, when considered independently, the dorsal and ventral muscle activation waves exhibit classical neuromechanical phase lag (NPL).

Five-nanometer ZnSn2O4:Cr,Eu ultra-small nanoparticles as new near infrared-emitting persistent luminescent nanoprobes for cellular and deep tissue imaging at 800 nm

Nanoscale ◽  
2017 ◽  
Vol 9 (25) ◽  
pp. 8631-8638 ◽  
Author(s):  
Jin-Lei Li ◽  
Jun-Peng Shi ◽  
Cheng-Cheng Wang ◽  
Peng-Hui Li ◽  
Zhen-Feng Yu ◽  
...  
Keyword(s):  
Near Infrared ◽  
Tissue Imaging ◽  
Deep Tissue ◽  
Deep Tissue Imaging ◽  
Small Nanoparticles

Schematic illustration of the synthesis, functionalization and repeated in vivo simulated deep tissue imaging of ZSO NPLNPs.

scholarly journals Genetically encoded calcium indicators for multi-color neural activity imaging and combination with optogenetics

2013 ◽  
Vol 6 ◽  
Author(s):  
Jasper Akerboom ◽  
Nicole Carreras Calderón ◽  
Lin Tian ◽  
Sebastian Wabnig ◽  
Matthias Prigge ◽  
...  
Keyword(s):  
Neural Activity ◽  
Calcium Indicators ◽  
Genetically Encoded Calcium Indicators
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