scholarly journals Sticky-flares: real-time tracking of mRNAs... or of endosomes?

2015 ◽  
Author(s):  
David Mason ◽  
Raphael Levy
Keyword(s):  
Real Time ◽  
Live Cells ◽  
Letter To The Editor ◽  
Real Time Tracking

This article is a Letter to the Editor of PNAS (Response to Briley et al, Quantification and real time tracking of RNA in live cells using Sticky flares. Proc Natl Acad Sci U S A 112(31), 9591).

Quantification and Real-Time Tracking of RNA in Live Cells Using Sticky-Flares*

2020 ◽  
pp. 1761-1774
Author(s):  
William E. Briley ◽  
Madison H. Bondy ◽  
Pratik S. Randeria ◽  
Torin J. Dupper ◽  
Chad A. Mirkin
Keyword(s):  
Real Time ◽  
Live Cells ◽  
Real Time Tracking

Quantification and Real-Time Tracking of RNA in Live Cells Using Sticky-Flares*

2020 ◽  
pp. 1761-1774
Author(s):  
William E. Briley ◽  
Madison H. Bondy ◽  
Pratik S. Randeria ◽  
Torin J. Dupper ◽  
Chad A. Mirkin
Keyword(s):  
Real Time ◽  
Live Cells ◽  
Real Time Tracking

Self-stacking of exfoliated charged nanosheets of LDHs and graphene as biosensor with real-time tracking of dopamine from live cells

2019 ◽  
Vol 1047 ◽  
pp. 197-207 ◽  
Author(s):  
Ayesha Aziz ◽  
Muhammad Asif ◽  
Muhammad Azeem ◽  
Ghazala Ashraf ◽  
Zhengyun Wang ◽  
...  
Keyword(s):  
Real Time ◽  
Live Cells ◽  
Real Time Tracking

scholarly journals Quantification and real-time tracking of RNA in live cells using Sticky-flares

2015 ◽  
Vol 112 (31) ◽  
pp. 9591-9595 ◽  
Author(s):  
William E. Briley ◽  
Madison H. Bondy ◽  
Pratik S. Randeria ◽  
Torin J. Dupper ◽  
Chad A. Mirkin
Keyword(s):  
Real Time ◽  
Spatiotemporal Analysis ◽  
Fluorescent Labeling ◽  
Live Cells ◽  
Small Nuclear Rna ◽  
Rna Transcripts ◽  
Spherical Nucleic Acid ◽  
Actin Mrna ◽  
Real Time Tracking ◽  
Target Rna

We report a novel spherical nucleic acid (SNA) gold nanoparticle conjugate, termed the Sticky-flare, which enables facile quantification of RNA expression in live cells and spatiotemporal analysis of RNA transport and localization. The Sticky-flare is capable of entering live cells without the need for transfection agents and recognizing target RNA transcripts in a sequence-specific manner. On recognition, the Sticky-flare transfers a fluorophore-conjugated reporter to the transcript, resulting in a turning on of fluorescence in a quantifiable manner and the fluorescent labeling of targeted transcripts. The latter allows the RNA to be tracked via fluorescence microscopy as it is transported throughout the cell. We use this novel nanoconjugate to analyze the expression level and spatial distribution of β-actin mRNA in HeLa cells and to observe the real-time transport of β-actin mRNA in mouse embryonic fibroblasts. Furthermore, we investigate the application of Sticky-flares for tracking transcripts that undergo more extensive compartmentalization by fluorophore-labeling U1 small nuclear RNA and observing its distribution in the nucleus of live cells.

Quantification and Real-Time Tracking of RNA in Live Cells Using Sticky-Flares*

2020 ◽  
pp. 1761-1774
Author(s):  
William E. Briley ◽  
Madison H. Bondy ◽  
Pratik S. Randeria ◽  
Torin J. Dupper ◽  
Chad A. Mirkin
Keyword(s):  
Real Time ◽  
Live Cells ◽  
Real Time Tracking

Real-time tracking of hydrogen peroxide secreted by live cells using MnO2 nanoparticles intercalated layered doubled hydroxide nanohybrids

2015 ◽  
Vol 898 ◽  
pp. 34-41 ◽  
Author(s):  
Muhammad Asif ◽  
Ayesha Aziz ◽  
Anh Quang Dao ◽  
Abdul Hakeem ◽  
Haitao Wang ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Real Time ◽  
Live Cells ◽  
Mno2 Nanoparticles ◽  
Real Time Tracking

An Overview of Data Aggregation Architecture for Real-Time Tracking with Sensor Networks

2006 ◽  
Author(s):  
Tian He ◽  
Lin Gu ◽  
Liqian Luo ◽  
Ting Yan ◽  
John A. Stankovic ◽  
...  
Keyword(s):  
Sensor Networks ◽  
Real Time ◽  
Data Aggregation ◽  
Real Time Tracking
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