scholarly journals RNA polymerase errors cause splicing defects and can be regulated by differential expression of RNA polymerase subunits

2015 ◽  
Author(s):  
Lucas B. Carey
Keyword(s):  
Rna Polymerase ◽  
Differential Expression ◽  
Splicing Defects ◽  
Rna Polymerase Subunits

scholarly journals RNA polymerase errors cause splicing defects and can be regulated by differential expression of RNA polymerase subunits

eLife ◽  
2015 ◽  
Vol 4 ◽  
Author(s):  
Lucas B Carey
Keyword(s):  
Rna Polymerase ◽  
Differential Expression ◽  
Dna Polymerase ◽  
Error Rate ◽  
Single Cells ◽  
Coding Sequences ◽  
Polymerase Fidelity ◽  
Splicing Defects ◽  
Cellular Phenotypes ◽  
Rna Polymerase Subunits

Errors during transcription may play an important role in determining cellular phenotypes: the RNA polymerase error rate is >4 orders of magnitude higher than that of DNA polymerase and errors are amplified >1000-fold due to translation. However, current methods to measure RNA polymerase fidelity are low-throughout, technically challenging, and organism specific. Here I show that changes in RNA polymerase fidelity can be measured using standard RNA sequencing protocols. I find that RNA polymerase is error-prone, and these errors can result in splicing defects. Furthermore, I find that differential expression of RNA polymerase subunits causes changes in RNA polymerase fidelity, and that coding sequences may have evolved to minimize the effect of these errors. These results suggest that errors caused by RNA polymerase may be a major source of stochastic variability at the level of single cells.

scholarly journals Involvement of Hsp90 in Assembly and Nuclear Import of Influenza Virus RNA Polymerase Subunits

2006 ◽  
Vol 81 (3) ◽  
pp. 1339-1349 ◽  
Author(s):  
Tadasuke Naito ◽  
Fumitaka Momose ◽  
Atsushi Kawaguchi ◽  
Kyosuke Nagata
Keyword(s):  
Influenza Virus ◽  
Rna Polymerase ◽  
Nuclear Transport ◽  
Intracellular Localization ◽  
Viral Rna ◽  
Polymerase Complex ◽  
Virus Rna ◽  
Binary Complexes ◽  
Infected Cells ◽  
Rna Polymerase Subunits

ABSTRACT Transcription and replication of the influenza virus RNA genome occur in the nuclei of infected cells through the viral RNA-dependent RNA polymerase consisting of PB1, PB2, and PA. We previously identified a host factor designated RAF-1 (RNA polymerase activating factor 1) that stimulates viral RNA synthesis. RAF-1 is found to be identical to Hsp90. Here, we examined the intracellular localization of Hsp90 and viral RNA polymerase subunits and their molecular interaction. Hsp90 was found to interact with PB2 and PB1, and it was relocalized to the nucleus upon viral infection. We found that the nuclear transport of Hsp90 occurs in cells expressing PB2 alone. The nuclear transport of Hsp90 was in parallel with that of the viral RNA polymerase binary complexes, either PB1 and PB2 or PB1 and PA, as well as with that of PB2 alone. Hsp90 also interacted with the binary RNA polymerase complex PB1-PB2, and it was dissociated from the PB1-PB2 complex upon its association with PA. Furthermore, Hsp90 could form a stable PB1-PB2-Hsp90 complex prior to the formation of a ternary polymerase complex by the assembly of PA in the infected cells. These results suggest that Hsp90 is involved in the assembly and nuclear transport of viral RNA polymerase subunits, possibly as a molecular chaperone for the polymerase subunits prior to the formation of a mature ternary polymerase complex.

scholarly journals Microprocessor Recruitment to Elongating RNA Polymerase II Is Required for Differential Expression of MicroRNAs

Cell Reports ◽  
2017 ◽  
Vol 20 (13) ◽  
pp. 3123-3134 ◽  
Author(s):  
Victoria A. Church ◽  
Sigal Pressman ◽  
Mamiko Isaji ◽  
Mary Truscott ◽  
Nihal Terzi Cizmecioglu ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Differential Expression

The RNA polymerase subunits E/F from the Antarctic archaeon Methanococcoides burtonii bind to specific species of mRNA

2010 ◽  
Vol 13 (8) ◽  
pp. 2039-2055 ◽  
Author(s):  
Davide De Francisci ◽  
Stefano Campanaro ◽  
Geoff Kornfeld ◽  
Khawar S. Siddiqui ◽  
Timothy J. Williams ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Specific Species ◽  
The Antarctic ◽  
Rna Polymerase Subunits

scholarly journals Archaeal RNA polymerase subunits E and F are not required for transcriptionin vitro, but aThermococcus kodakarensismutant lacking subunit F is temperature-sensitive

2008 ◽  
Vol 70 (3) ◽  
pp. 623-633 ◽  
Author(s):  
Akira Hirata ◽  
Tamotsu Kanai ◽  
Thomas J. Santangelo ◽  
Momoko Tajiri ◽  
Kenji Manabe ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Temperature Sensitive ◽  
Rna Polymerase Subunits

scholarly journals Differential expression of cucumber RNA-dependent RNA polymerase 1 genes during antiviral defence and resistance

2017 ◽  
Vol 19 (2) ◽  
pp. 300-312 ◽  
Author(s):  
Diana Leibman ◽  
Michael Kravchik ◽  
Dalia Wolf ◽  
Sabrina Haviv ◽  
Mira Weissberg ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Differential Expression ◽  
Rna Dependent Rna Polymerase ◽  
Rna Polymerase 1
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