scholarly journals Development of EST-SSR annotated database in olive (Olea europaea)

2015 ◽  
Author(s):  
sami said adawy ◽  
morad mokhtar mokhtar ◽  
Alsamman Mahmoud Alsamman ◽  
Mahmoud m Sakr

Olive tree (Olea europaea L.) is one of the most important oil producing crops in the world and the genetic identification of several genotypes by using molecular markers is the first step in its breeding programs. A set of 1,801 well-informative EST-SSR primers targeting specific Olive genes included in different biological processes and pathways were generated using 11,215 Olive EST sequences acquired from the NCBI database. Our bioinformatics analytical procedure showed that 8295 SSR motifs were detected which belonged to different motif types with occurrences of 77.6%, 11.84%, 8.62%, 0.84%, 0.77% and 0.29% for Mononucleotide, trinucleotide, dinucleotide, hexanucleotide, pentanucleotide and tetranucleotide respectively. The appearance of the AAG/CTT repeat was highly represented in trinucleotide and the representation of AG/CT was high in dinucleotide repeats. Results obtained from functional annotation of olives EST sequences targeted with our primers set indicated that 78.5% of these sequences having homology with known proteins, while 4.2% was homologous to hypothetical, predicted, unnamed or uncharacterized proteins and the 17.3% sequences did not possess homology with any known proteins. Our EST-SSR primer set cover a total of 92 biological pathways such as carbohydrate metabolism pathway, energy metabolism & carbon fixation in photosynthetic organism pathway including 11 pathways associated with lipid metabolism .A twenty five randomly selected primers were applied to 9 Egyptian cultivated olive accessions to test its amplification and polymorphism detection efficacy. All tested primers were successfully amplified and only 10 exhibited detectable polymorphism.

2020 ◽  
Vol 10 (18) ◽  
pp. 6414
Author(s):  
Nuno Rodrigues ◽  
Teresa Pinho ◽  
Susana Casal ◽  
António M. Peres ◽  
Paula Baptista ◽  
...  

Oleaster (Olea europaea var. sylvestris), or the wild olive tree, has great interest as a source of genetic material for olive breeding programs. Nevertheless, information about its oil composition is scarce. In the present work, the characterization of oleaster fruit morphology and oil chemical composition from three different tree populations in Northeastern Portugal (Moncorvo, Alijó and Vila Nova de Foz Côa) was performed. The three studied populations presented some morphological differences in the fruits, but similar oil chemical composition. Oleic acid (68.9–70.6%) was the most abundant fatty acid. High variability was observed in total tocopherol content, ranging between 263 and 503 mg/kg. Additionally, high amounts of total sterols were found, from 1742 to 2198 mg/kg of oil. A rich composition in phenols was found with 14 phenolic compounds identified. The evaluated parameters for oleander oils allowed discriminating the oils according to the geographical origin and were consistent with those commonly found in olive oil, showing that they are particularly rich in antioxidants and can be exploited in breeding programs to increase the amounts of bioactive compounds in cultivated oils.


2011 ◽  
pp. 89-94 ◽  
Author(s):  
G. Psarras ◽  
I. Kasapakis ◽  
E. Stefanoudaki ◽  
I. Papadakis ◽  
K.S. Chartzoulakis

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Giuseppe Russo ◽  
Isacco Beritognolo ◽  
Marina Bufacchi ◽  
Vitale Stanzione ◽  
Andrea Pisanelli ◽  
...  

2020 ◽  
Vol 8 (9) ◽  
pp. 1321 ◽  
Author(s):  
Rosario Nicoletti ◽  
Claudio Di Vaio ◽  
Chiara Cirillo

In addition to the general interest connected with investigations on biodiversity in natural contexts, more recently the scientific community has started considering occurrence of endophytic fungi in crops in the awareness of the fundamental role played by these microorganisms on plant growth and protection. Crops such as olive tree, whose management is more and more frequently based on the paradigm of sustainable agriculture, are particularly interested in the perspective of a possible applicative employment, considering that the multi-year crop cycle implies a likely higher impact of these symbiotic interactions. Aspects concerning occurrence and effects of endophytic fungi associated with olive tree (Olea europaea) are revised in the present paper.


Genes ◽  
2020 ◽  
Vol 11 (10) ◽  
pp. 1171
Author(s):  
Paula Moreno-Sanz ◽  
Luca Lombardo ◽  
Silvia Lorenzi ◽  
Franco Michelotti ◽  
Maria Stella Grando

The area of the Garda Lake within the Trentino province (north of Italy) is the northernmost part of Europe where the Mediterranean species Olea europaea L. is traditionally cultivated. ‘Casaliva’ is claimed as the main variety traditionally grown in the Garda Trentino area (GT) from which a world renowned niche extra virgin olive oil is produced. Since a dominant presence of ‘Casaliva’ would link the fruit set success and yield to a self-pollination compatibility system, a deep genetic survey of the olive tree population in the GT has been performed with the aim of establishing the actual varietal composition and of understanding from which pollen donor the ‘Casaliva’ olives originate. Forty-four different genetic profiles were observed among the 205 leaf samples collected from 106 ancient trees through the analysis of 20 nuclear microsatellite markers. The varietal composition in modern orchards was also explored and the vast majority of the additional 151 trees analyzed showed the same genotype as the ancient accessions of ‘Casaliva’. The results support the long historical link of ‘Casaliva’ with the GT and, besides a high varietal homogeneity, they also revealed the presence of olive genetic resources essential to fruit production. In fact, the parentage analysis of 550 embryos from drupes of ‘Casaliva’ evidenced that a cross-fertilization system is favored and a list of candidate cultivars most suitable as local pollinizers of ‘Casaliva’ was identified.


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