scholarly journals Particle size distribution and optimal capture of aqueous macrobial eDNA

2014 ◽  
Author(s):  
Cameron R. Turner ◽  
Matthew A. Barnes ◽  
Charles C.Y. Xu ◽  
Stuart E. Jones ◽  
Christopher L. Jerde ◽  
...  
Keyword(s):  
Particle Size ◽  
Particle Size Distribution ◽  
Pore Size ◽  
Size Distribution ◽  
Suspended Particle ◽  
Survey Method ◽  
Water Volume ◽  
Size Analysis ◽  
Future Research ◽  
Gravimetric Analysis

1. Detecting aquatic macroorganisms with environmental DNA (eDNA) is a new survey method with broad applicability. However, the origin, state, and fate of aqueous macrobial eDNA - which collectively determine how well eDNA can serve as a proxy for directly observing organisms and how eDNA should be captured, purified, and assayed - are poorly understood. 2. The size of aquatic particles provides clues about their origin, state, and fate. We used sequential filtration size fractionation to measure, for the first time, the particle size distribution (PSD) of macrobial eDNA, specifically Common Carp (hereafter referred to as Carp) eDNA. We compared it to the PSDs of total eDNA (from all organisms) and suspended particle matter (SPM). We quantified Carp mitochondrial eDNA using a custom qPCR assay, total eDNA with fluorometry, and SPM with gravimetric analysis. 3. In a lake and a pond, we found Carp eDNA in particles from >180 to <0.2 µm, but it was most abundant from 1-10 µm. Total eDNA was most abundant below 0.2 µm and SPM was most abundant above 100 µm. SPM was ≤0.1% total eDNA, and total eDNA was ≤0.0004% Carp eDNA. 0.2 µm filtration maximized Carp eDNA capture (85%±6%) while minimizing total (i.e., non-target) eDNA capture (48%±3%), but filter clogging limited this pore size to a volume <250 mL. To mitigate this limitation we estimated a continuous PSD model for Carp eDNA and derived an equation for calculating isoclines of pore size and water volume that yield equivalent amounts of Carp eDNA. 4. Our results suggest that aqueous macrobial eDNA predominantly exists inside mitochondria or cells, and that settling plays an important role in its fate. For optimal eDNA capture, we recommend 0.2 µm filtration or a combination of larger pore size and water volume that exceeds the 0.2 µm isocline. In situ filtration of large volumes could maximize detection probability when surveying large habitats for rare organisms. Our method for eDNA particle size analysis enables future research to compare the PSDs of eDNA from other organisms and environments, and to easily apply them for ecological monitoring.

Experimental investigation of the effect of particle size distribution of suspended particles on microfiltration

1996 ◽  
Vol 34 (9) ◽  
pp. 133-140 ◽  
Author(s):  
J. S. Chang ◽  
L. J. Tsai ◽  
S. Vigneswaran
Keyword(s):  
Experimental Investigation ◽  
Particle Size ◽  
Particle Size Distribution ◽  
Pore Size ◽  
Size Distribution ◽  
Suspended Particle ◽  
Permeate Flux ◽  
Effect Of Particle Size ◽  
Main Factors ◽  
Suspended Particle Size

Suspended particle size is one of the main factors which affect the performance of the microfiltration. Experimental results with bi and trimodal suspensions of latex particles clearly showed that the permeate flux and the quality were significantly affected by the particle size and its distribution, especially when the particle size was smaller than the pore size of the membrane.

X-ray fluorescence spectrometry for rapid screening of particle size in soils

2021 ◽  
Author(s):  
Maame Croffie ◽  
Paul N. Williams ◽  
Owen Fenton ◽  
Anna Fenelon ◽  
Karen Daly
Keyword(s):  
Particle Size ◽  
Particle Size Distribution ◽  
Size Distribution ◽  
Soil Texture ◽  
Particle Size Analysis ◽  
Parent Material ◽  
Fluorescence Spectrometry ◽  
Rapid Screening ◽  
Size Analysis ◽  
X Ray

&lt;p&gt;Soil texture is an essential factor for effective land management in agricultural production. Knowledge of soil texture and particle size at field scale can aid with on-going soil management decisions. Standard soil physical and gravimetric methods for particle size analysis are time-consuming and X-ray fluorescence spectrometry (XRF) provides a rapid and cost-effective alternative. The objective of this study was to explore the use of XRF as a predictor for particle size. An extensive archive of Irish soils with particle size and soil texture data was used to select samples for XRF analysis. Regression and correlation analyses on XRF determined results showed that the relationship between Rb and % clay varied with soil type and was dependent on the parent material. There was a strong relationship (R &gt; 0.62, R&lt;sup&gt;2&lt;/sup&gt;&gt;0.30, p&lt;0.05) between Rb and clay for soils originating from bedrock such as limestones and slate. Contrastingly, no significant relationship (R&lt;0.03, R&lt;sup&gt;2&lt;/sup&gt;=0.00, p&gt;0.05) exists between Rb and % clay for soils originating from granite and gneiss. Furthermore, there was a significant negative correlation (p&lt;0.05) between Rb and % sand. The XRF is a useful technique for rough screening of particle size distribution in soils originating from certain parent materials. Thus, this may contribute to the rapid prediction of soil texture based on knowledge of the particle size distribution.&lt;/p&gt;&lt;p&gt;&amp;#160;&lt;/p&gt;

Determination of atmospheric aerosol particle size distribution and visibility using a mobile laboratory

1982 ◽  
Vol 60 (8) ◽  
pp. 1101-1107
Author(s):  
C. V. Mathai ◽  
A. W. Harrison
Keyword(s):  
Particle Size ◽  
Particle Size Distribution ◽  
Size Distribution ◽  
Atmospheric Aerosols ◽  
Suspended Particle ◽  
Aerosol Size Distribution ◽  
Size Distributions ◽  
Mobile Laboratory ◽  
Mean Values ◽  
The City

As part of an ongoing general research program on the effects of atmospheric aerosols on visibility and its dependence on aerosol size distributions in Calgary, this paper presents the results of a comparative study of particle size distribution and visibility in residential (NW) and industrial (SE) sections of the city using a mobile laboratory. The study was conducted in the period October–December, 1979. An active scattering aerosol spectrometer measured the size distributions and the corresponding visibilities were deduced from scattering coefficients measured with an integrating nephelometer.The results of this transit study show significantly higher suspended particle concentrations and reduced visibilities in the SE than in the NW. The mean values of the visibilities are 44 and 97 km for the SE and the NW respectively. The exponent of R (particle radius) in the power law aerosol size distribution has a mean value of −3.36 ± 0.24 in the SE compared with the corresponding value of −3.89 ± 0.39 for the NW. These results arc in good agreement with the observations of Alberta Environment; however, they are in contradiction with a recent report published by the City of Calgary.

scholarly journals Log-Cubic Method for Generation of Soil Particle Size Distribution Curve

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Songhao Shang
Keyword(s):  
Particle Size ◽  
Particle Size Distribution ◽  
Size Distribution ◽  
Cross Validation ◽  
Interpolation Method ◽  
Physical Property ◽  
Particle Size Analysis ◽  
Soil Particle ◽  
Size Analysis ◽  
Particle Analysis

Particle size distribution (PSD) is a fundamental physical property of soils. Traditionally, the PSD curve was generated by hand from limited data of particle size analysis, which is subjective and may lead to significant uncertainty in the freehand PSD curve and graphically estimated cumulative particle percentages. To overcome these problems, a log-cubic method was proposed for the generation of PSD curve based on a monotone piecewise cubic interpolation method. The log-cubic method and commonly used log-linear and log-spline methods were evaluated by the leave-one-out cross-validation method for 394 soil samples extracted from UNSODA database. Mean error and root mean square error of the cross-validation show that the log-cubic method outperforms two other methods. What is more important, PSD curve generated by the log-cubic method meets essential requirements of a PSD curve, that is, passing through all measured data and being both smooth and monotone. The proposed log-cubic method provides an objective and reliable way to generate a PSD curve from limited soil particle analysis data. This method and the generated PSD curve can be used in the conversion of different soil texture schemes, assessment of grading pattern, and estimation of soil hydraulic parameters and erodibility factor.

scholarly journals Preparation and Characterization of Latex Particles as Potential Physical Shale Stabilizer in Water-Based Drilling Fluids

2014 ◽  
Vol 2014 ◽  
pp. 1-8
Author(s):  
Junyi Liu ◽  
Zhengsong Qiu ◽  
Wei’an Huang ◽  
Dingding Song ◽  
Dan Bao
Keyword(s):  
Particle Size ◽  
Particle Size Distribution ◽  
Methyl Methacrylate ◽  
Size Distribution ◽  
Pore Pressure ◽  
Water Medium ◽  
Gravimetric Analysis ◽  
Latex Particles ◽  
Pressure Transmission ◽  
Ft Ir

The poly(styrene-methyl methacrylate) latex particles as potential physical shale stabilizer were successfully synthesized with potassium persulfate as an initiator in isopropanol-water medium. The synthesized latex particles were characterized by Fourier transform infrared spectroscopy (FT-IR), particle size distribution measurement (PSD), transmission electron microscopy (TEM), and thermal gravimetric analysis (TGA). FT-IR and TGA analysis confirmed that the latex particles were prepared by polymerization of styrene and methyl methacrylate and maintained good thermal stability. TEM and PSD analysis indicated that the spherical latex particles possessed unimodal distribution from 80 nm to 345 nm with the D90 value of 276 nm. The factors influencing particle size distribution (PSD) of latex particles were also discussed in detail. The interaction between latex particles and natural shale cores was investigated quantitatively via pore pressure transmission tests. The results indicated that the latex particles as potential physical shale stabilizer could be deformable to bridge and seal the nanopores and microfractures of shale to reduce the shale permeability and prevent pore pressure transmission. What is more, the latex particles as potential physical shale stabilizer work synergistically with chemical shale stabilizer to impart superior shale stability.

Size Reduction of Clay Particles in Nanometer Dimensions

2002 ◽  
Vol 740 ◽  
Author(s):  
Gopinath Mani ◽  
Qinguo Fan ◽  
Samuel C. Ugbolue ◽  
Isabelle M. Eiff
Keyword(s):  
Particle Size ◽  
Particle Size Distribution ◽  
Specific Surface ◽  
Size Distribution ◽  
Research Work ◽  
Particle Size Analysis ◽  
Laser Diffraction ◽  
Size Analysis ◽  
Clay Particles ◽  
Nano Size

ABSTRACTThis research work focuses on combining ball milling and ultrasonication to produce nano-size clay particles. Our work also emphasizes on increasing the specific surface area of montmorillonite clay particles by reducing the particle size to nanometer dimensions. We have characterized the as-received clay particles by using particle size analysis based on laser diffraction and found that the size of the clay particles is not consistent and the particle size distribution is very broad. However, after the unique treatment and processing, the clay particles were obtained in nanometer dimensions with narrowed particle size distribution.

scholarly journals Effects of Particle Size Distribution on the Properties of Natural-Based Composite

2019 ◽  
Vol 4 (4) ◽  
pp. 170-177
Author(s):  
J. Abutu ◽  
S.A. Lawal ◽  
M.B. Ndaliman ◽  
R.A. Lafia-Araga ◽  
A.S. Abdulrahman
Keyword(s):  
Particle Size ◽  
Particle Size Distribution ◽  
Size Distribution ◽  
Treatment Time ◽  
Particle Size Analysis ◽  
Coconut Shell ◽  
Size Analysis ◽  
Distribution Width ◽  
Testing Procedures ◽  
Coconut Shells

In this study, locally sourced natural materials (coconut shells and seashells) were used separately to produce composites. The powders were sieved with sieve size of 10 µm and characterized using a particle size analyser (DLS) in order to ascertain their particle size distribution. Also, the effects of particle size distribution on the performance of sourced coconut shells and seashells-based composite was investigated. About 52% of the characterized powder was afterward used along with other ingredients (35% binder, 8% alumina and 5% graphite) to produced composites using moulding pressure (14 MPa), moulding temperature (160 ºC), curing time (12 min) and heat treatment time (1 hr). The performance of the composites was thereafter evaluated using standard testing procedures. The results of particle size analysis indicated that the seashell powder (0.27) possesses lower distribution width (PDI) compared to the coconut shell powder (0.342) while the coconut shell (542.3 nm) showed lower Z-average diameter compared to the seashell powder (1096 nm) with some little traces of nanoparticles (<10 µm). Also, the experimental results obtained from composite characterization indicated that the coconut shell-based samples exhibited better performance in terms of its mechanical and tribological properties compared to the seashell-based samples.

scholarly journals Particle size distribution of fly ash from co-incineration of bituminous coal with municipal solid waste

2018 ◽  
Vol 28 ◽  
pp. 01008
Author(s):  
Ewelina Cieślik ◽  
Tomasz Konieczny ◽  
Bartłomiej Bobik
Keyword(s):  
Particle Size ◽  
Fly Ash ◽  
Particle Size Distribution ◽  
Solid Waste ◽  
Size Distribution ◽  
Bituminous Coal ◽  
Municipal Waste ◽  
Test Material ◽  
Size Analysis ◽  
Exhaust Gas

One of the source of air pollutants is emission from local coal-fired boiler-houses and domestic heating boilers. The consequence of incineration of municipal waste is the introduction of additional pollutants into the atmosphere, including fly ash. The aim of this work was to evaluate the particle size distribution of fly ash emitted by coal combustion and co-incineration of coal with municipal waste in a domestic 18 kW central heating boiler equipped with an automatic fuel feeder. Mixtures of bituminous coal with different types of solid waste (5, 10 and 15% of mass fraction) were used. Solid waste types consisted of: printed, colored PE caps, fragmented cable trunking, fragmented car gaskets and shredded tires from trucks. During the incineration of a given mixture of municipal waste with bituminous coal, the velocity of exhaust gas was specified, the concentration and mass flow of fly ash were determined together with the physico-chemical parameters of the exhaust gas, the samples of emitted fly ash were taken as the test material. Particle size analysis of fly ash was performed using laser particle sizer Fritch Analysette 22. The PM10 share from all fly ashes from incineration of mixtures was about 100%. Differences were noted between PM2.5 and PM1.

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