ROS accumulation in cotton ovule epidermal cells is necessary for fiber initiation

2013 ◽  
Author(s):  
mingxiong pang ◽  
Nickolas Sanford ◽  
Thea Wilkins
Keyword(s):  
Epidermal Cells ◽  
Cotton Leaf ◽  
Fiber Initiation ◽  
Leaf Trichomes ◽  
H2o2 Accumulation ◽  
Ros Accumulation ◽  
Cotton Ovule ◽  
Seed Epidermis ◽  
Leaf Trichome

Cotton (Gossypium hirsutum) fiber, an extremely elongated and thickened single cell of the seed epidermis, is the world’s most important natural and economical textile fiber. Unlike Arabidopsis leaf trichomes, fiber initials are randomly developed and frequently form in adjacent seed epidermal cells and follow no apparent pattern. Numerous publications suggested cotton fiber development shares a similar mechanism with Arabidopsis leaf trichome development. Here we show that H2O2 accumulation in cotton ovule epidermal cells by NBT staining ovules at different development stages between TM1 and N1n2, a lintless-fuzzless doubled mutant originated from TM1. In contrast, Arabidopsis and cotton leaf trichomes do not show H2O2 content. By adding DPI (H2O2 inhibitor) and SHAM (H2O2 activator) in vitro ovule cultures, we show fiber initiation directly involves with H2O2 accumulation. We propose that the directional accumulation of H2O2 in cotton ovule epidermal cell is the drive for fiber initiation, elongation.

scholarly journals Similarities between Initiation Mechanism between Cotton Fiber and Arabidopsis Trichome: Prospects in Improving Cotton Fiber Yield

2020 ◽  
pp. 1-9
Author(s):  
Anh Phu Nam Bui ◽  
Binh-Nguyen Ong ◽  
Thuan-Anh Nguyen Huu ◽  
Hoang-Dung Tran
Keyword(s):  
Cotton Fiber ◽  
Textile Industry ◽  
Functional Characterization ◽  
Model System ◽  
Epidermal Cells ◽  
Comprehensive Understanding ◽  
Initiation Mechanism ◽  
Fiber Initiation ◽  
Leaf Trichome

Cotton fiber is the fundamental material for a textile industry, and currently there is an immense interest in understanding the process of fiber initiation and development. Cotton fiber, also known as seed trichome, is differentiated from the seed coat epidermal cells similar to Arabidopsis leaf trichome, which is differentiated from the leaf epidermal cells. Despite functional characterization of individual cotton fiber initiation genes, currently there is not a comprehensive understanding of the mechanism behind cotton fiber initiation. Since the resemblance in initiation to cotton fiber, the Arabidopsis trichome has been successfully employed as a model system for functional characterization of cotton fiber initiation genes. Knowledge gained from the initiation mechanism of Arabidopsis trichomes will facilitate, as a comparative model system in understanding of the cotton fiber initiation mechanisms.

Epithelial–mesenchymal interactions allow for epidermal cells to display an in vivo-like phenotype in vitro

2005 ◽  
Vol 73 (2-3) ◽  
pp. 79-87 ◽  
Author(s):  
Eduardo Mitrani ◽  
Guy Nadel ◽  
Eilat Hasson ◽  
Esther Harari ◽  
Yael Shimoni
Keyword(s):  
Epidermal Cells

scholarly journals Benzo[f]indole-4,9-dione Derivatives Effectively Inhibit the Growth of Triple-Negative Breast Cancer

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4414
Author(s):  
Fabiana Sélos Guerra ◽  
Flaviana Rodrigues Fintelman Dias ◽  
Anna Claudia Cunha ◽  
Patricia Dias Fernandes
Keyword(s):  
Breast Cancer ◽  
Triple Negative Breast Cancer ◽  
Triple Negative ◽  
Cancer Cell Line ◽  
Caspase 9 ◽  
Apoptosis Pathway ◽  
Indole Compounds ◽  
Intrinsic Apoptosis Pathway ◽  
Ros Accumulation

Triple-negative breast cancer (TNBC) is a subtype of breast cancer with poor clinical outcome, and currently no effective targeted therapies are available. Indole compounds have been shown to have potential antitumor activity against various cancer cells. In the present study, we found that new four benzo[f]indole-4,9-dione derivatives reduce TNBC cell viability by reactive oxygen species (ROS) accumulation stress in vitro. Further analyses showed that LACBio1, LACBio2, LACBio3 and LACBio4 exert cytotoxic effects on MDA-MB 231 cancer cell line by inducing the intrinsic apoptosis pathway, activating caspase 9 and Bax/Bcl-2 pathway in vitro. These results provide evidence that these new four benzo[f]indole-4,9-dione derivatives could be potential therapeutic agents against TNBC by promoting ROS stress-mediated apoptosis through intrinsic-pathway caspase activation.

In vitro analysis of interactions between sensory neurons and skin: evidence for selective innervation of dermis and epidermis

Development ◽  
1985 ◽  
Vol 86 (1) ◽  
pp. 53-70
Author(s):  
J.-M. Verna
Keyword(s):  
Ganglion Cells ◽  
Neurite Growth ◽  
Epidermal Cells ◽  
Serum Free Medium ◽  
Diffusible Factor ◽  
Vitro Analysis ◽  
Dermal Cells ◽  
Dorsal Root Ganglion Cells ◽  
In Vitro Analysis

Axons from dorsal root ganglion cells cultured in a serum-free medium on poly-L-lysine or collagen substrates interact differently with dermis and epidermis. The orientation of neurite growth is not changed by encountering mesenchymal cells migrating from the outgrowth zone of a dermal explant, and neurites form close membrane associations with some dermal cells; in contrast, neurites strongly avoid epidermis and deviate around the edge of an epidermal explant. When cultures are grown on polylysine this avoidance behaviour occurs at a distance from the epidermis. It is suppressed in the presence of necrotic epidermal cells. We suggest that this avoidance is due to epidermal diffusible factor(s) which bind preferentially to polylysine. The possibility of an absence of specific recognition cues between neurites and epidermal cells is discussed.

scholarly journals Adaptability and leaf anatomical features in oil palm seedlings produced by embryo rescue and pre-germinated seeds

2010 ◽  
Vol 22 (3) ◽  
pp. 209-215 ◽  
Author(s):  
Zanderluce G. Luis ◽  
Kadja Milena G. Bezerra ◽  
Jonny Everson Scherwinski-Pereira
Keyword(s):  
Oil Palm ◽  
Embryo Rescue ◽  
Morphological Characteristics ◽  
Growth Conditions ◽  
Epidermal Cells ◽  
In Vitro Cultivation ◽  
Vascular Bundles ◽  
Anatomical Plasticity ◽  
Germinated Seeds

Changes in the leaf structure of plants grown in different conditions have been reported, such as increase in size and density of stomata and reduction in stomatal control, amount of epicuticular wax, and mesophyll thickness, with a high diversity of intercellular spaces. However, these changes are highly variable depending on the physiological and morphological characteristics of each species. The objective of this work was to analyze the adaptability and anatomical plasticity of oil palm seedlings produced after embryo rescue and pre-germinated seeds. Expanded leaves were prepared for evaluation of morphometric data and anatomical structures. It was verified that the environmental conditions in vitro negatively influenced the stomata density, epidermal and hypodermal thickness, and the values for the expansion cells and leaf mesophile. Anatomically, the oil palm leaves present the same tissues composition in both growth conditions, with uniseriate epidermal cells, and tetracitic stomata occurring in both epidermal surfaces. Epidermal cells from in vitro plants are thinner than ones from greenhouse. The midrib of leaves from greenhouse plants are more developed and is composed by only one central vascular bundle, while plants from in vitro cultivation developed three to four collateral vascular bundles.

scholarly journals Interaction of integrins alpha 3 beta 1 and alpha 2 beta 1: potential role in keratinocyte intercellular adhesion.

1993 ◽  
Vol 120 (2) ◽  
pp. 523-535 ◽  
Author(s):  
B E Symington ◽  
Y Takada ◽  
W G Carter
Keyword(s):  
Potential Role ◽  
Intercellular Adhesion ◽  
Epidermal Cells ◽  
Intercellular Contact ◽  
In Vitro Experiments ◽  
Selective Binding ◽  
Contact Sites ◽  
Do So

The colocalization of integrins alpha 2 beta 1 and alpha 3 beta 1 at intercellular contact sites of keratinocytes in culture and in epidermis suggests that these integrins may mediate intercellular adhesion (ICA). P1B5, an anti-alpha 3 beta 1 mAb previously reported to inhibit keratinocyte adhesion to epiligrin, was also found to induce ICA. Evidence that P1B5-induced ICA was mediated by alpha 2 beta 1 and alpha 3 beta 1 was obtained using both ICA assays and assays with purified, mAb-immobilized integrins. Selective binding of alpha 2 beta 1-coated beads to epidermal cells or plate-bound alpha 3 beta 1 was observed. This binding was inhibited by mAbs to integrin alpha 3, alpha 2, or beta 1 subunits and could be stimulated by P1B5. We also demonstrate a selective and inhibitable interaction between affinity-purified integrins alpha 2 beta 1 and alpha 3 beta 1. Finally, we show that expression of alpha 2 beta 1 by CHO fibroblasts results in the acquisition of collagen and alpha 3 beta 1 binding. Binding to both of these ligands is inhibited by P1H5, an anti-alpha 2 beta 1 specific mAb. Results of these in vitro experiments suggest that integrins alpha 2 beta 1 and alpha 3 beta 1 can interact and may do so to mediate ICA in vivo. Thus, alpha 3 beta 1 mediates keratinocyte adhesion to epiligrin and plays a second role in ICA via alpha 2 beta 1.

scholarly journals Modulation of G6PD affects bladder cancer via ROS accumulation and the AKT pathway in vitro

2018 ◽  
Author(s):  
Xiaoyi Chen ◽  
Zhijie Xu ◽  
Zhijian Zhu ◽  
Anqi Chen ◽  
Guanghou Fu ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Akt Pathway ◽  
Ros Accumulation

scholarly journals Trichomes Morphology and Essential Oils Characterization of Field-Growing and In Vitro Propagated Plants of Lavandula pedunculata

2008 ◽  
Vol 14 (S3) ◽  
pp. 148-149 ◽  
Author(s):  
M. Zuzarte ◽  
A.M. Dinis ◽  
C. Cavaleiro ◽  
J. Canhoto ◽  
L. Salgueiro
Keyword(s):  
Chemical Composition ◽  
Antifungal Activity ◽  
Essential Oils ◽  
Vegetative Propagation ◽  
Leaf Trichomes ◽  
Economic Exploitation ◽  
Selection Of

The selection of native Lavandula species and their economic exploitation have increased in the last few years. Micropropagation techniques have been used as an alternative for vegetative propagation allowing the multiplication of selected genotypes and chemotypes. Our previous studies showed that the essential oils of Lavandula pedunculata have an important antifungal activity against dermatophyte strains. Therefore, a new line of investigation concerning the in vitro culture of this species is justified. In the present study we compare the morphology of the leaf trichomes and the chemical composition of their essential oils in both field-growing and in vitro propagated plants.

The mitotic pattern of the embryonic epidermis of chick during scale morphogenesis

Development ◽  
1969 ◽  
Vol 21 (2) ◽  
pp. 235-242
Author(s):  
Takesi Yohro
Keyword(s):  
Chick Embryo ◽  
Experimental Studies ◽  
Gross Anatomy ◽  
Epidermal Cells ◽  
Mitotic Count ◽  
Proliferation Rate ◽  
In Vitro Studies ◽  
White Leghorn ◽  
Developmental Problems

The epidermis of the chick embryo has been widely used for in vitro studies of many developmental problems (Matoltsy, 1960; Billingham & Silvers, 1963). The present attempt to determine the proliferation rate of chick embryonic epidermal cells was expected to provide a base for experimental studies, but a preliminary mitotic count revealed that the number of mitoses varied greatly in different areas. This suggested accumulation of mitoses in some restricted parts of the epidermis, and so a mapping experiment was carried out to determine the distribution of mitoses in this material. The characteristic mitotic pattern which was discovered is described and discussed. About 300 White Leghorn embryos were used: 20 for study of the gross anatomy of scales, 200 for Colcemid treatment and 80 for [3H] thymidine treatment.

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