scholarly journals High-Throughput Protein Expression and Purification for Proteomics Research

2002 ◽  
Vol 2 ◽  
pp. 146-147
Author(s):  
Sharon A. Doyle ◽  
Michael Murphy ◽  
Jennifer Primus ◽  
Paul Richardson ◽  
Trevor Hawkins
Keyword(s):  
Protein Expression ◽  
High Throughput ◽  
Expression And Purification ◽  
Protein Expression And Purification ◽  
Proteomics Research

scholarly journals Polyionic and cysteine-containing fusion peptides as versatile protein tags

2013 ◽  
Vol 394 (8) ◽  
pp. 995-1004 ◽  
Author(s):  
Hauke Lilie ◽  
Susanne Richter ◽  
Sabine Bergelt ◽  
Stefan Frost ◽  
Franziska Gehle
Keyword(s):  
Protein Expression ◽  
Specific Protein ◽  
Fusion Peptides ◽  
Protein Fusion ◽  
Expression And Purification ◽  
Functionalized Surfaces ◽  
Protein Expression And Purification ◽  
Bifunctional Proteins ◽  
Proteomics Research ◽  
Protein Tags

Abstract In response to advances in proteomics research and the use of proteins in medical and biotechnological applications, recombinant protein production and the design of specific protein characteristics and functions has become a widely used technology. In this context, protein fusion tags have been developed as indispensable tools for protein expression, purification, and the design of functionalized surfaces or artificially bifunctional proteins. Here we summarize how positively or negatively charged polyionic fusion peptides with or without an additional cysteine can be used as protein tags for protein expression and purification, for matrix-assisted refolding of aggregated protein, and for coupling of proteins either to technologically relevant matrices or to other proteins. In this context we used cysteine-containing polyionic fusion peptides for the design of immunotoxins. In general, polyionic fusion tags can be considered as a multifunctional module in protein technology.

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