IN VITRO BIOSYNTHESIS OF AN AI-2 LIKE BACTERIAL AUTOINDUCER BY RECOMBINANT ESCHERICHIA COLI ENZYMES

2002 ◽  
Author(s):  
Michael Ramm ◽  
Silke Steinbach ◽  
Volker Schroeckh
Keyword(s):  
Escherichia Coli ◽  
Recombinant Escherichia Coli ◽  
In Vitro Biosynthesis

In vitro biosynthesis of functional Escherichia coli su3+tyrosine transfer RNA

Biochemistry ◽  
1977 ◽  
Vol 16 (16) ◽  
pp. 3608-3618 ◽  
Author(s):  
Maurille J. Fournier ◽  
Elizabeth Webb ◽  
Shirley Tang
Keyword(s):  
Escherichia Coli ◽  
Transfer Rna ◽  
In Vitro Biosynthesis

scholarly journals Evaluation of biological safety in vitro and immunogenicity in vivo of recombinant Escherichia coli Shiga toxoids as candidate vaccines in cattle

2015 ◽  
Vol 46 (1) ◽  
Author(s):  
Katharina Kerner ◽  
Philip S Bridger ◽  
Gabriele Köpf ◽  
Julia Fröhlich ◽  
Stefanie Barth ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Recombinant Escherichia Coli ◽  
Biological Safety

scholarly journals Poly(3-hydroxyalkanoate) polymerase synthesis and in vitro activity in recombinant Escherichia coli and Pseudomonas putida

2005 ◽  
Vol 69 (3) ◽  
pp. 286-292 ◽  
Author(s):  
Qun Ren ◽  
Guy de Roo ◽  
Jan B. van Beilen ◽  
Manfred Zinn ◽  
Birgit Kessler ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Putida ◽  
Vitro Activity ◽  
Recombinant Escherichia Coli ◽  
In Vitro Activity

Recent discoveries in the pathways to cobalamin (coenzyme B12) achieved through chemistry and biology

2007 ◽  
Vol 79 (12) ◽  
pp. 2179-2188 ◽  
Author(s):  
A. Ian Scott ◽  
Charles A. Roessner
Keyword(s):  
Escherichia Coli ◽  
Side Chain ◽  
Coenzyme B12 ◽  
Over Expression ◽  
High Resolution Nmr ◽  
In Vitro Biosynthesis ◽  
Transport Complex ◽  
Aerobic And Anaerobic

The genetic engineering of Escherichia coli for the over-expression of enzymes of the aerobic and anaerobic pathways to cobalamin has resulted in the in vivo and in vitro biosynthesis of new intermediates and other products that were isolated and characterized using a combination of bioorganic chemistry and high-resolution NMR. Analyses of these products were used to deduct the functions of the enzymes that catalyze their synthesis. CobZ, another enzyme for the synthesis of precorrin-3B of the aerobic pathway, has recently been described, as has been BluB, the enzyme responsible for the oxygen-dependent biosynthesis of dimethylbenzimidazole. In the anaerobic pathway, functions have recently been experimentally confirmed for or assigned to the CbiMNOQ cobalt transport complex, CbiA (a,c side chain amidation), CbiD (C-1 methylation), CbiF (C-11 methylation), CbiG (lactone opening, deacylation), CbiP (b,d,e,g side chain amidation), and CbiT (C-15 methylation, C-12 side chain decarboxylation). The dephosphorylation of adenosylcobalamin-phosphate, catalyzed by CobC, has been proposed as the final step in the biosynthesis of adenosylcobalamin.

scholarly journals Glycolipid binding of purified and recombinant Escherichia coli produced verotoxin in vitro.

1987 ◽  
Vol 262 (18) ◽  
pp. 8834-8839
Author(s):  
C A Lingwood ◽  
H Law ◽  
S Richardson ◽  
M Petric ◽  
J L Brunton ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Recombinant Escherichia Coli
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