OSTEOPONTIN REGULATES CD44 EXPRESSION AND IS REQUIRED FOR NORMAL MACROPHAGE/OSTEOCLAST FUNCTION

2002 ◽  
Author(s):  
Baoqian Zhu ◽  
Keiko Suzuki ◽  
Harvey A. Goldberg ◽  
Susan R. Rittling ◽  
David T. Denhardt ◽  
...  
Keyword(s):  
Cd44 Expression ◽  
Osteoclast Function ◽  
Normal Macrophage

The role of transcription factor Egr‐1 in IL‐1 up‐regulation of tubular CD44 expression

Nephrology ◽  
2000 ◽  
Vol 5 (3) ◽  
pp. A92-A92
Author(s):  
Takazoe K ◽  
Foti R ◽  
Hurst La ◽  
Atkins Rc ◽  
Nikolic‐Paterson DJ.
Keyword(s):  
Transcription Factor ◽  
Cd44 Expression

Pathophysiological implication of Autotaxin on osteoclast function

2016 ◽  
Author(s):  
Sacha Flammier ◽  
Tristan Gicquel ◽  
Francois Duboeuf ◽  
Olivier Peyruchaud ◽  
Fabienne Coury ◽  
...  
Keyword(s):  
Osteoclast Function

Paget’s disease: clinical update

2011 ◽  
Vol 152 (33) ◽  
pp. 1337-1346
Author(s):  
Judit Donáth ◽  
Gyula Poór
Keyword(s):  
Alkaline Phosphatase ◽  
Serum Alkaline Phosphatase ◽  
Paget’S Disease ◽  
Paget's Disease ◽  
Etiologic Role ◽  
Osteoblast Activity ◽  
Chronic Disorder ◽  
Abnormal Increase ◽  
Osteoclast Function ◽  
High Level

Paget’s disease is a chronic disorder of bone remodeling, characterized by an abnormal increase of osteoclast and, hence, osteoblast activity. The imbalance of bone turnover results in the formation of unhealthy and fragile bone. It also leads to impairment of adjacent joints and to a risk of various complications. Current research focuses on the elucidation of the etiologic role viral infection and predisposing genetic factors. Paget’s disease is commonly discovered by chance; its suspicion is raised either by high level of alkaline phosphatase or by the X-ray of the pathological bone. Bisphosphonates have proven to be effective in controlling disease activity because they inhibit osteoclast function. Their use is recommended when bone-derived serum alkaline phosphatase is high and/or when disease localizations are highly suspected for the development of complications. Orv. Hetil., 2011, 152, 1337–1346.

scholarly journals 89Zr anti-CD44 immuno-PET monitors  CD44 expression on splenic myeloid cells and HT29 colon cancer cells

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jin Won Park ◽  
Kyung-Ho Jung ◽  
Jin Hee Lee ◽  
Seung Hwan Moon ◽  
Young Seok Cho ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Nude Mice ◽  
Myeloid Cells ◽  
Human Antibody ◽  
Tumor Uptake ◽  
Colon Cancer Cells ◽  
Cell Surface Glycoprotein ◽  
Cd44 Expression ◽  
Human And Mouse

AbstractCD44 is a cell-surface glycoprotein involved in cell–cell interaction, adhesion, and migration. CD44 is found on colon cancer cells and on immune cells. Previous studies of 89Zr PET imaging of CD44 have relied on an anti-human antibody (Ab), which can influence biodistribution in murine models. In this study, we used an Ab that cross-reacts with both human and mouse origin CD44 of all isoforms to unveil the type of leukocyte responsible for high splenic anti-CD44 uptake and investigate how its regulation can influence tumor immuno-PET. The Ab was site-specifically labeled with 89Zr-deferoxamine on cysteine residues. 89Zr-anti-CD44 demonstrated high-specific binding to HT29 human colon cancer cells and monocytic cells that showed CD44 expression. When 89Zr-anti-CD44 was administered to Balb/C nude mice, there was remarkably high splenic uptake but low SNU-C5 tumor uptake (1.2 ± 0.7%ID/g). Among cells isolated from Balb/C mouse spleen, there was greater CD44 expression on CD11b positive myeloid cells than lymphocytes. In cultured monocytic and macrophage cells, LPS stimulation upregulated CD44 expression and increased 89Zr-anti-CD44 binding. Similarly, normal Balb/C mice that underwent lipopolysaccharide (LPS) stimulation showed a significant upregulation of CD44 expression on splenic myeloid cells. Furthermore, LPS treatment stimulated a 2.44-fold increase of 89Zr-anti-CD44 accumulation in the spleen, which was attributable to splenic myeloid cells. Finally, in Balb/C nude mice bearing HT29 tumors, we injected 89Zr-anti-CD44 with greater Ab doses to reduce binding to splenic cells. The results showed lower spleen uptake and improved tumor uptake (2.9 ± 1.3%ID/g) with a total of 300 μg of Ab dose, and further reduction of spleen uptake and greater tumor uptake (5.7 ± 0.0%ID/g) with 700 μg Ab dose. Thus, using an 89Zr labeled Ab that cross-reacts with both human and mouse CD44, we demonstrate that CD44 immuno-PET has the capacity to monitor CD44 regulation on splenic myeloid cells and may also be useful for imaging colon tumors.

scholarly journals Absence of Dap12 and the αvβ3 integrin causes severe osteopetrosis

2014 ◽  
Vol 208 (1) ◽  
pp. 125-136 ◽  
Author(s):  
Wei Zou ◽  
Steven L. Teitelbaum
Keyword(s):  
Bone Mass ◽  
Αvβ3 Integrin ◽  
Skeletal Phenotype ◽  
Osteoclast Function ◽  
Deficient Mice

In vitro, ligand occupancy of αvβ3 integrin induces phosphorylation of Dap12, which is essential for osteoclast function. Like mice deleted of only αvβ3, Dap12−/− mice exhibited a slight increase in bone mass, but Dap12−/− mice, lacking another ITAM protein, FcRγ, were severely osteopetrotic. The mechanism by which FcRγ compensates for Dap12 deficiency is unknown. We find that co-deletion of FcRγ did not exacerbate the skeletal phenotype of β3−/− mice. In contrast, β3/Dap12 double-deficient (DAP/β3−/−) mice (but not β1/Dap12 double-deficient mice) were profoundly osteopetrotic, reflecting severe osteoclast dysfunction relative to those lacking αvβ3 or Dap12 alone. Activation of OSCAR, the FcRγ co-receptor, rescued Dap12−/− but not DAP/β3−/−osteoclasts. Thus, the absence of αvβ3 precluded compensation for Dap12 deficiency by FcRγ. In keeping with this, Syk phosphorylation did not occur in OSCAR-activated DAP/β3−/− osteoclasts. Thus, FcRγ requires the osteoclast αvβ3 integrin to normalize the Dap12-deficient skeleton.

OP - CD44 EXPRESSION AND ITS ASSOCIATION TO CLINICOPATHOLOGICAL FEATURES AND SURVIVAL IN ORAL SQUAMOUS CELL CARCINOMA

2017 ◽  
Vol 123 (2) ◽  
pp. e38
Author(s):  
SANDRA VENTORIN VON ZEIDLER ◽  
JOSÉ ROBERTO VASCONCELOS DE PODESTÁ ◽  
DANIELY SOUZA DE NARDI ◽  
PRISCILA MARINHO ABREU ◽  
MARCELLA SOL ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Clinicopathological Features ◽  
Cd44 Expression

A new mutation in the KINDLIN-3 gene ablates integrin-dependent leukocyte, platelet, and osteoclast function in a patient with leukocyte adhesion deficiency-III

2015 ◽  
Vol 62 (9) ◽  
pp. 1677-1679 ◽  
Author(s):  
Roman Crazzolara ◽  
Kathrin Maurer ◽  
Harald Schulze ◽  
Barbara Zieger ◽  
Jozef Zustin ◽  
...  
Keyword(s):  
Leukocyte Adhesion ◽  
Leukocyte Adhesion Deficiency ◽  
New Mutation ◽  
Osteoclast Function
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