scholarly journals Lateral Surgical Approach to Lumbar Intervertebral Discs in an Ovine Model

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
David Oehme ◽  
Tony Goldschlager ◽  
Jeffrey Rosenfeld ◽  
Andrew Danks ◽  
Peter Ghosh ◽  
...  
Keyword(s):  
Animal Model ◽  
Lumbar Spine ◽  
Intervertebral Discs ◽  
Lateral Approach ◽  
Large Animal Model ◽  
Anterolateral Approach ◽  
Large Animal ◽  
Ovine Model ◽  
Far Lateral Approach ◽  
Far Lateral

The sheep is becoming increasingly used as a large animal model for preclinical spine surgery studies. Access to the ovine lumbar intervertebral discs has traditionally been via an anterior or anterolateral approach, which requires larger wound incisions and, at times, significant abdominal retraction. We present a new minimally invasive operative technique for a far-lateral approach to the ovine lumbar spine that allows for smaller incisions, excellent visualisation of intervertebral discs, and minimal abdominal retraction and is well tolerated by animals with minimal morbidity.

scholarly journals A Nonthoracotomy Myocardial Infarction Model in an Ovine Using Autologous Platelets

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Tyler Spata ◽  
Daniel Bobek ◽  
Bryan A. Whitson ◽  
Sampath Parthasarathy ◽  
Peter J. Mohler ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Animal Model ◽  
Myocardial Infarct ◽  
Border Zone ◽  
Large Animal Model ◽  
Large Animal ◽  
Ovine Model ◽  
Intravascular Thrombosis ◽  
Serial Serum ◽  
Male Sheep

Objective. There is a paucity of a biological large animal model of myocardial infarction (MI). We hypothesized that, using autologous-aggregated platelets, we could create an ovine model that was reproducible and more closely mimicked the pathophysiology of MI.Methods. Mepacrine stained autologous platelets from male sheep (n=7) were used to create a myocardial infarction via catheter injection into the mid-left anterior descending (LAD) coronary artery. Serial daily serum troponin measurements were taken and tissue harvested on post-embolization day three. Immunofluorescence microscopy was used to detect the mepacrine-stained platelet-induced thrombus, and histology performed to identify three distinct myocardial (infarct, peri-ischemic “border zone,” and remote) zones.Results. Serial serum troponin levels (μg/mL) measured0.0±0.0at baseline and peaked at297.4±58.0on post-embolization day 1, followed by153.0±38.8on day 2 and76.7±19.8on day 3. Staining confirmed distinct myocardial regions of inflammation and fibrosis as well as mepacrine-stained platelets as the cause of intravascular thrombosis.Conclusion. We report a reproducible, unique model of a biological myocardial infarction in a large animal model. This technique can be used to study acute, regional myocardial changes following a thrombotic injury.

scholarly journals Neotropical Rattlesnake (Crotalus simus) Venom Pharmacokinetics in Lymph and Blood Using an Ovine Model

Toxins ◽  
2020 ◽  
Vol 12 (7) ◽  
pp. 455 ◽  
Author(s):  
Edgar Neri-Castro ◽  
Melisa Bénard-Valle ◽  
Dayanira Paniagua ◽  
Leslie V. Boyer ◽  
Lourival D. Possani ◽  
...  
Keyword(s):  
Animal Model ◽  
Snake Venom ◽  
Serine Proteases ◽  
Large Animal Model ◽  
Large Animal ◽  
Clinical Effects ◽  
Differential Absorption ◽  
Protein Families ◽  
Ovine Model ◽  
Viper Venoms

The most abundant protein families in viper venoms are Snake Venom Metalloproteases (SVMPs), Snake Venom Serine Proteases (SVSPs) and Phospholipases (PLA2s). These are primarily responsible for the pathophysiology caused by the bite of pit-vipers; however, there are few studies that analyze the pharmacokinetics (PK) of whole venom (WV) and its protein families. We studied the pathophysiology, PK profile and differential absorption of representative toxins from venom of Neotropical Rattlesnake (Crotalus simus) in a large animal model (ovine). Toxins studied included crotoxin (the main lethal component), which causes moderate to severe neurotoxicity; SVSPs, which deplete fibrinogen; and SVMPs, which cause local tissue damage and local and systemic hemorrhage. We found that Whole Venom (WV) was highly bioavailable (86%) 60 h following intramuscular (IM) injection, and extrapolation suggests that bioavailability may be as high as 92%. PK profiles of individual toxins were consistent with their physicochemical properties and expected clinical effects. Lymph cannulated animals absorbed 1.9% of WV through lymph during the first 12 h. Crotoxin was minimally detectable in serum after intravenous (IV) injection; however, following IM injection it was detected in lymph but not in blood. This suggests that crotoxin is quickly released from the blood toward its tissue targets.

scholarly journals Pig lumbar spine anatomy and imaging-guided lateral lumbar puncture: A new large animal model for intrathecal drug delivery

2013 ◽  
Vol 216 (1) ◽  
pp. 10-15 ◽  
Author(s):  
Josef Pleticha ◽  
Timothy P. Maus ◽  
Christian Jeng-Singh ◽  
Michael P. Marsh ◽  
Fadi Al-Saiegh ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Animal Model ◽  
Lumbar Spine ◽  
Lumbar Puncture ◽  
Large Animal Model ◽  
Large Animal ◽  
Intrathecal Drug Delivery

Surgical Relevance of Pediatric Skull Base Maturation for the Far-Lateral Approach

2019 ◽  
Author(s):  
Robert Rennert ◽  
Reid Hoshide ◽  
Michael Brandel ◽  
Jeffrey Steinberg ◽  
Joel Martin ◽  
...  
Keyword(s):  
Skull Base ◽  
Lateral Approach ◽  
Far Lateral Approach ◽  
Far Lateral

Extended Transpterygoid Endonasal Far Lateral Approach: The Role of the Eustachian Tube Revised

Skull Base ◽  
2009 ◽  
Vol 19 (01) ◽  
Author(s):  
Paolo Battaglia ◽  
Guglielmo Romano ◽  
Iacopo Dallan ◽  
Maurizio Bignami ◽  
Luca Muscatello ◽  
...  
Keyword(s):  
Eustachian Tube ◽  
Lateral Approach ◽  
Far Lateral Approach ◽  
Far Lateral

Medulla oblongata cavernoma removal through a lazy far lateral approach: operative video and technical nuances

2019 ◽  
Vol 1 (2) ◽  
pp. V21
Author(s):  
Carlos Candanedo ◽  
Samuel Moscovici ◽  
Sergey Spektor
Keyword(s):  
Medulla Oblongata ◽  
Cavernous Malformation ◽  
Complete Removal ◽  
Lateral Approach ◽  
Neurological Deficits ◽  
Total Resection ◽  
Postoperative Mri ◽  
Far Lateral Approach ◽  
Far Lateral ◽  
Brainstem Cavernous Malformation

Removal of brainstem cavernous malformation remains a surgical challenge. We present a case of a 63-year-old female who was diagnosed with a large cavernoma located in the medulla oblongata. The patient suffered three episodes of brainstem bleeding resulting in significant neurological deficits (hemiparesis, dysphagia, and dysarthria). It was decided to remove the cavernoma through a left-sided modified far lateral approach.3The operative video demonstrates the surgical steps and nuances of a complete removal of this complex medulla oblongata cavernous malformation. Total resection was achieved without complications. Postoperative MRI revealed no signs of residual cavernoma with clinical improvement.The video can be found here: https://youtu.be/BTtMvvLMOFM.

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