scholarly journals Antimicrobial Activity and Phytochemical Screening ofBuchenavia tetraphylla(Aubl.) R. A. Howard (Combretaceae: Combretoideae)

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Ygor Lucena Cabral de Oliveira ◽  
Luís Cláudio Nascimento da Silva ◽  
Alexandre Gomes da Silva ◽  
Alexandre José Macedo ◽  
Janete Magali de Araújo ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Mycobacterium Smegmatis ◽  
Micrococcus Luteus ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Proteus Vulgaris ◽  
Hydroalcoholic Extract ◽  
Phytochemical Constituents ◽  
New Perspective ◽  
First Time

This study evaluated the antimicrobial and hemolytic activities and phytochemical constituents of hydroalcoholic extract and its fractions fromBuchenavia tetraphyllaleaves. Cyclohexane (BTCF), ethyl acetate (BTEF), and n-butanol-soluble (BTSBF) and non-soluble (BTNBF) fractions were obtained from a liquid-liquid partition of hydroalcoholic extract (BTHE) fromB. tetraphyllaleaves. The hemolytic activity of active fractions was checked. The BTHE inhibited the growth ofMicrococcus luteus(MIC: 0.10 mg/mL),Pseudomonas aeruginosa(MIC: 0.20 mg/mL),Mycobacterium smegmatis(MIC: 0.39 mg/mL),Proteus vulgaris,andStaphylococcus aureus(MIC: 0.78 mg/mL for both). The more active fractions were BTCF and BTBSF. BTCF showed better potential to inhibitM. luteus(0.10 mg/mL),P. aeruginosa(0.20 mg/mL),S. enteritidis(0.39 mg/mL), andS. aureus(1.56 mg/mL). BTBSF showed the best results forM. luteus(0.10 mg/mL),M. smegmatis,B. subtilis(0.39 mg/mL for both), andP. vulgaris(0.10 mg/mL). The HC50 were greater than observed MIC: 20.30, 4.70 and 2.53 mg/mL, respectively, to BTBF, BTHE and BTCF, which. The phytochemical analysis detected the presence of flavanoids, triterpene, carbohydrate, and tannin. Our work showed for the first time the broad-spread antimicrobial activity ofB. tetraphylla, which has nonhemolytic action, creating a new perspective on the interesting association of traditional and scientific knowledge.

In-vitro anti-bacterial activity and phytochemical screening of crude extracts of Catharanthus roseus L. (G.) Don.

2017 ◽  
Vol 37 (2) ◽  
Author(s):  
Manish Kapoor ◽  
Jyoti Rani ◽  
Rupinder Kaur
Keyword(s):  
Antimicrobial Activity ◽  
Petroleum Ether ◽  
Mycobacterium Smegmatis ◽  
Cardiac Glycosides ◽  
Methanolic Extract ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Bacterial Strains ◽  
Aqueous Methanol

Present study aims to investigate phytochemical analysis as well as in vitro antimicrobial activity of crude aqueous, methanol, ethanol, chloroform and petroleum ether extracts from leaves of C. roseus. Antimicrobial activity of extract was studied against various bacterial strains (Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, Staphylococcus aureus and Mycobacterium smegmatis). Qualitative preliminary phytochemical analysis revealed that alkaloids, phenols, flavonoids, amino acid and cardiac glycosides are present in the extracts. The result for total phenol and flavonoids content was the highest in methanol and the lowest in petroleum ether crude extract. The study revealed that inhibition significantly depend upon the solvent used for extraction and bacterial strain tested for susceptibility. Aqueous extracts were found less efficient as compared to organic solvent. Methanolic extract were found more effective against tested microbes.

Preliminary phytochemical screening, isolation and structural elucidation of chloroform leaf extracts of Maesa lanceolata

2019 ◽  
Author(s):  
Chem Int
Keyword(s):  
Cardiac Glycosides ◽  
Leaf Extract ◽  
Chloroform Extract ◽  
Phytochemical Analysis ◽  
Spectroscopic Methods ◽  
Phytochemical Screening ◽  
Leaf Extracts ◽  
Standard Procedures ◽  
Phytochemical Constituents ◽  
First Time

The aim of this study was to screen the phytochemical constituents, isolate and elucidate the structure of chloroform extracts from the leaves of Maesa lanceolata. The qualitative phytochemical analysis of the leaf extract of Maesa lanceolata was done following standard procedures and the tests revealed the presence of cardiac glycosides and saponins in the crude extracts. The chloroform extract of the leaves of M. lanceolata led to the isolation of one compound, C1. Structure determination was accomplished by means of spectroscopic methods (IR, 13C and 1H NMR). According to the spectral data (IR, 1HNMR, 13CNMR and DEPT), probably Myrsenene was identified which is reported for the first time from M. lanceolata.

scholarly journals PRELIMINARY PHYTOCHEMICAL SCREENING AND IN VITRO ANTIMICROBIAL ACTIVITY OF ETHANOLIC EXTRACT OF WHOLE AERIAL PART OF THE HERB LEUCAS PLUKENETII SPRENG (FAMILY-LAMINACEAE)

2017 ◽  
Vol 9 (3) ◽  
pp. 87
Author(s):  
Uttam Borah ◽  
Biswajit Dash ◽  
Suvakanta Dash ◽  
Lawrence Kalita
Keyword(s):  
Antimicrobial Activity ◽  
Aerial Part ◽  
Ethanolic Extract ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Bacterial Strains ◽  
Standard Methods ◽  
Aerial Parts ◽  
Phytochemical Constituents

Objective: To evaluate the antimicrobial activity of ethanolic extract of Leucas Plukenetii Spreng (family-laminaceae) in conjugation with phytochemical analysis.Methods: The ethanolic extract of whole aerial parts of the herb Leucas Plukenetiispreng (family-laminaceae) was prepared and analysed for phytochemical constituents using standard methods. The antimicrobial activity of the plant extract was examined against 2 bacterial strains among one is gram positive and other is gram negative and 2 fungal strains using agar well diffusion method.Results: The present investigation shows the phytochemical analysis, antimicrobial activity of the ethanolic extract of the plant Leucas Plukenetii Spreng. Various phytochemical analyses revealed the presence of alkaloids, saponin, flavonoids, carbohydrates, glycosides, Steroids, proteins and amino acids and tannins. The antimicrobial activity of the ethanolic extract of the plant showed significant result against all the of the test organisms.Conclusion: The present study concluded that ethanolic extract of whole aerial parts of the Leucas Plukenetii Spreng contains the high presence of phytochemicals. The ethnaolic extract of the plant was found to possess promising antimicrobial activity when compared with the standards.

scholarly journals Phytochemical screening and antimicrobial activity testing of crude hydroalcoholic extract from leaves of Sphagneticola trilobata (Asteraceae)

2019 ◽  
Vol 49 (4) ◽  
Author(s):  
Ana Greice Borba Leite ◽  
Eulina Tereza Nery Farias ◽  
Ana Paula de Oliveira ◽  
Ruan Emmanuell Franco de Abreu ◽  
Mateus Matiuzzi da Costa ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Secondary Metabolites ◽  
Human Skin ◽  
Phytochemical Analysis ◽  
Microbiological Analysis ◽  
Phytochemical Screening ◽  
Hydroalcoholic Extract ◽  
Skin Bacteria ◽  
Broth Microdilution Method ◽  
Staphylococcus Spp

ABSTRACT: This study aimed to perform phytochemical analysis and to test the antimicrobial activity of the crude hydroalcoholic extract obtained from the leaves of Sphagneticola trilobata. Classes of secondary metabolites present in the extract were identified through phytochemical screening using analytical thin-layer chromatography. Antimicrobial activity was evaluated by testing cultures of Staphylococcus aureus, S. epidermidis, Staphylococcus spp., Escherichia coli, Serratia marcescens, Enterococcus faecalis, Pseudomonas aeruginosa, Salmonella Typhimurium, and Klebsiella pneumoniae isolated from human skin and those of Staphylococcus spp. isolated from dog skin using the broth microdilution method. In the phytochemical screening, classes of anthracenic derivatives and mono-, sesqui-, and diterpenes were identified. Colorimetric analysis showed total phenol and total flavonoid contents of 21.7 ± 0.009 mg of gallic acid equivalents per gram of sample and 0.23 ± 0.005 mg of catechin equivalents per gram of sample, respectively. Microbiological analysis revealed that the hydroalcoholic extract of S. trilobata exhibited antimicrobial activity against cultures of Staphylococcus spp., E. coli, S. marcescens, and E. faecalis isolated from human skin and those of Staphylococcus spp. isolated from dog skin. Thus, crude hydroalcoholic extract of leaves of S. trilobata contained flavonoids and terpenoids as secondary metabolites, which contributed to its antimicrobial activity against skin bacteria isolated from different sources.

scholarly journals PRELIMINARY PHYTOCHEMICAL SCREENING AND ANTIMICROBIAL ACTIVITY OF ETHANOLIC EXTRACT OF STEMS OF IPOMOEA AQUATICA FORSK. (FAMILY-CONVOLVULACEAE)

2017 ◽  
Vol 9 (4) ◽  
pp. 109
Author(s):  
Imdadul Haque Chaudhury ◽  
Trishna Das ◽  
Dipankar Saha
Keyword(s):  
Antimicrobial Activity ◽  
Ethanolic Extract ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Bacterial Strains ◽  
Standard Methods ◽  
Ipomoea Aquatica ◽  
Ethanolic Extracts ◽  
Phytochemical Constituents ◽  
Ipomoea Aquatic

Objective: To evaluate the antimicrobial activity ethanolic extracts of stems of the plant Ipomoea aquatica Forsk. (family-Convolvulaceae) along with preliminary phytochemical analysis.Methods: The ethanolic extract of stems of the plant Ipomoea aquatica Forsk. (family-Convolvulaceae) were prepared and analyzed for phytochemical constituents using standard methods. The antimicrobial activity of the plant extracts was examined against 2 bacterial strains among one is gram positive and other is gram negative and 2 fungus using agar well diffusion method.Results: The present experiment shows the phytochemical analysis, antimicrobial activity of the ethanolic extract of stems of the plant Ipomoea aquatic Forsk. (Family-Convolvulaceae). Various phytochemical analysis revealed the presence of alkaloids, saponin, flavonoids, carbohydrates, glycosides, Steroids, proteins and amino acids and tannins. All the extracts of the plant showed antimicrobial activity against most of the test organisms.Conclusion: The present study concluded that ethanolic extract of stems of the plant Ipomoea aquatica Forsk. Contain the high presence of phytochemical. The ethanolic extract of the plant was found to possess promising antimicrobial activity when compared with the standards. 

scholarly journals PHYTOCHEMICAL CONSTITUENTS OF THE ROOT OF BERBERIS MICROPHYLLA

2017 ◽  
Vol 10 (6) ◽  
pp. 225 ◽  
Author(s):  
María Cristina Furrianca ◽  
Marysol Alvear ◽  
Tomás Zambrano ◽  
Víctor Fajardo ◽  
Luis Salazar
Keyword(s):  
Secondary Metabolites ◽  
Cardiac Glycosides ◽  
Methanolic Extract ◽  
Ethanolic Extract ◽  
Phytochemical Screening ◽  
Qualitative And Quantitative ◽  
Medicinal Properties ◽  
Phytochemical Constituents ◽  
First Time

Objective: To objective of this work was to perform phytochemical qualitative and quantitative analyzes of the main secondary metabolites in the root of Berberis microphylla.Methods: The extracts of B. microphylla root were tested through phytochemical screening and the quantification of the most important constituents was carried out using spectrophotometric and gravimetric techniques.Results: Phytochemical screening of both extracts showed the presence of alkaloids, flavonoids, glycosides, cardiac glycosides, saponins, terpenes,and tannins, which are pharmacologically important. Quantification of the major phytochemicals groups showed that the ethanolic extract contains 3.9% alkaloids, 0.46% flavonoids, 9.53% tannins, and 3.60% saponins. Similarly, the methanolic extract contains 6.61% alkaloids, 0.41% flavonoids, 7.40% tannins, and 1.43% saponins.Conclusion: This is the first time that the presence of tannins, flavonoids, and saponins in this plant has been reported. The medicinal properties of the root of B. microphylla may exist due to the presence secondary metabolites.

scholarly journals Quantitative and Qualitative Identification of Phytochemical Constituents of Sida Rhombifolia Leaves Extract

2019 ◽  
Vol 8 (2S4) ◽  
pp. 403-408
Keyword(s):  
Quantitative Analysis ◽  
Cardiac Glycosides ◽  
Total Content ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Biological Compounds ◽  
Potential Sources ◽  
Pharmaceutical Industries ◽  
Phytochemical Constituents ◽  
Sida Rhombifolia

Anti-inflammatory, analgesic, antimicrobial, anti-arthritic, antibacterial, antispasmodic, hypoglycaemic and hepatoprotective characteristics of Sida rhombifolia are included in the Malvaceae family. Most of the plant belongs to the Malvaceae family, as they comprise a number of phytochemicals and biological compounds, are potential sources of different medications. This is study on Sida rhombifolia leaf extract's tophytochemical constituen ts. Phytochemical screening results in herbal stan-dardizat ion and preparation and may relate the components to thei r medicinal / pharmacological uses. The qualitative phytochemical analysis has shown that the extract is positive for saponins, flavonoids, alkaloids, phenols and same extract is negative for carbohydrate, tannins, glycosides, cardiac glycosides, terpenoids, coumarins, steroid& phytosteroids, phlobutanins, anthraquinones. Quantitative analysis of phytochemicals includes the estimation of flavonoid, tannin and total content of phenol. The result suggest that the Sida rhombifolia leaves extract consist plenty of phytochemicals beneficial in alternative medical and pharmaceutical industries.

scholarly journals Novel Flavonoid Glycosides of Quercetin from Leaves and Flowers of Gaiadendron punctatum G.Don. (Violeta de Campo), used by the Saraguro Community in Southern Ecuador, Inhibit α-Glucosidase Enzyme

Molecules ◽  
2019 ◽  
Vol 24 (23) ◽  
pp. 4267
Author(s):  
Cedeño ◽  
Espinosa ◽  
Andrade ◽  
Cartuche ◽  
Malagón
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Competitive Inhibition ◽  
Micrococcus Luteus ◽  
Optical Rotation ◽  
2D Nmr ◽  
Flavonoid Glycosides ◽  
Hydroalcoholic Extract ◽  
Indigenous Group ◽  
Southern Ecuador

Gaiadandendron punctatum G.Don. (violeta de campo) is a plant used in traditional medicine by the Saraguro people, an ancient indigenous group that lives in southern Ecuador. From samples collected in the region, six glycoside flavonoids, five with quercetin and one with kaempferol as aglycon, were isolated and characterized from hydroalcoholic extracts of leaves and flowers. Rutin (2) was found in flowers and leaves, nicotiflorin (1) was found in flowers, artabotryside A (3) was found in leaves, and three novel quercetin flavonoid glycosides were isolated, elucidated, and characterized via 1D and 2D NMR experiments (1H, 13C, COSY, DEPT, HMBC, HSQC, TOCSY, NOESY, ROESY), acid hydrolysis–derivatization–GC-MS analysis, HPLC-MS, IR, UV, and optical rotation. The new quercetin flavonoid glycosides were named hecpatrin (4) (isolated from leaves), gaiadendrin (5) (isolated from leaves), and puchikrin (6) (isolated from flowers). The hydroalcoholic extracts of the leaves presented antimicrobial activity against Micrococcus luteus, Staphylococcus aureus, and Enterococcus faecalis and the hydroalcoholic extract of the flowers was active against Micrococcus luteus. However, glycoside flavonoids presented scarce antimicrobial activity against bacteria. Hydroalcoholic extracts from leaves and flowers and their secondary metabolites showed inhibition against the α-glucosidase enzyme at different concentrations. Rutin, gaiadendrin, and nicotiflorin showed competitive α-glucosidase inhibition, while hecpatrin presented non-competitive inhibition.

scholarly journals Phytochemical Analysis, Antioxidant Activity, Antimicrobial Activity, and Cytotoxicity of Chaptalia nutans Leaves

2020 ◽  
Vol 2020 ◽  
pp. 1-15
Author(s):  
Letiele Bruck de Souza ◽  
Amanda Leitão Gindri ◽  
Thainara de Andrade Fortes ◽  
Thais Felli Kubiça ◽  
Jefferson Enderle ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Clinical Isolate ◽  
Condensed Tannins ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Phytochemical Study ◽  
Carbonyl Protein ◽  
Crude Leaf Extract

Context. Chaptalia nutans (L.) Pol. (family: Asteraceae) is widely used in traditional medicine as laxative and anticough medications and especially in the traumatisms, wounds, and hemorrhages in topical preparations. Objective. This work was to evaluate the chemical constitution of the hydromethanolic (30/70 methanol-water) macerating extract obtained from the leaves of C. nutans, as well as to study the antioxidant, antimicrobial, cytotoxic, and genotoxic activity of the species. Materials and methods. Phytochemical screening, antioxidant activity (total phenolic, total flavonoid, condensed tannins content, DPPH radical, and FRAP), antibacterial activity (P. aeruginosa, B. cereus, E. epidermidis, E. coli, S. aureus, E. faecalis, P. mirabilis, Candida glabrata (clinical isolate), Candida tropicalis (clinical isolate), C. krusei (clinical isolate), and C. albicans (clinical isolate)), and oxidative stress parameters (TBARS, carbonyl protein, and DCFH) were analyzed according to the literature. Toxicity of C. nutans was evaluated using an alternative method, D. melanogaster, as well as a locomotor assay. Results. The phytochemical screening test of methanolic leaves extract revealed the presence of alkaloids, coumarins, quaternary bases, phenolics, flavonoids, tannins, and free steroids. A quantitative phytochemical study indicated the total phenol (30.17 ± 1.44 mg/g), flavonoid (21.64 ± 0.66 mg/g), and condensed tannins (9.58 ± 0.99 mg/g). DPPH (345.41 ± 5.35 μg/mL) and FRAP (379.98 ± 39.25 μM FeSO4/mg sample) show to extract of C. nutans leaves an intermediate value, indicating moderate antioxidant activity of the extract. Antibacterial results revealed only a positive result (antimicrobial activity) for the hexane fraction which significantly inhibited the microorganisms E. epidermidis, C. tropicalis, C. glabrata, and C. krusei at a concentration of 1000 μg/mL. TBARS, carbonyl protein, and DCFH demonstrate that the extract has the ability to protect the cell from protein and lipid damage, as well as the inhibition of oxygen-derived radicals at the three concentrations tested: 0.1, 1, and 10 mg/mL. Regarding the toxicity of C. nutans extract against D. melanogaster, it was found that until the concentration of 15 mg/mL, the extract showed no toxicity and that the LC50 obtained was 24 mg/mL. Results show that the C. nutans extract leaves used to prevent PQ damage were effective in reducing flies’ mortality and improving locomotor capacity. Conclusion. Our studies demonstrated for the first time that C. nutans crude leaf extract has high antioxidant capacity both in vitro and in vivo through different analysis techniques. These results make it possible to infer future applications in the pharmacological area, evidenced by the low toxicity observed in D. melanogatser, as well as the ability to neutralize different sources of RONS.

Phytochemical Analysis and GC-MS Profiling in the Flower of Plumeria alba

2020 ◽  
Vol 981 ◽  
pp. 280-284
Author(s):  
Siti Aisyah Mohamad ◽  
Noor Suhana Adzahar ◽  
Muhammad Nadeem Akhtar ◽  
Seema Zareen ◽  
Thong Chuan Lee
Keyword(s):  
Chemical Constituents ◽  
Volatile Oil ◽  
Gas Chromatography Mass Spectrometry ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Therapeutic Properties ◽  
Phytochemical Constituents ◽  
Screening Assays ◽  
Major Chemical ◽  
Ethylhexyl Phthalate

Therapeutic properties of the medicinal plant are due to the presence of phytochemical constituents. The phytochemical constituents of Plumeria alba flower were investigated by phytochemical screening assays and gas chromatography-mass spectrometry (GC-MS). The phytochemical screening of hexane, dichloromethane, ethyl acetate, butanol, and aqueous extracts of P. alba flower showed it contains a various concentration of saponins, flavonoids, tannins, steroids, volatile oil and phenolic compounds. Several major chemical constituents that were identified is squalene, bis(2-ethylhexyl) phthalate, methyl (methyl 4-O-methyl-α-d-mannopyranoside) uronate and tricyclo [7.2.0.0(2,6)] undecan-5-ol, 2,6,10,10-tetramethyl- (isomer 2) by using GC-MS technique.

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