scholarly journals In VitroAntibacterial and Time-Kill Evaluation of theErythrina caffraThunb. Extract against Bacteria Associated with Diarrhoea

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Olufunmiso Olusola Olajuyigbe ◽  
Anthony Jide Afolayan
Keyword(s):  
Staphylococcus Aureus ◽  
Cell Count ◽  
Micrococcus Luteus ◽  
Ethanolic Extract ◽  
Viable Cell ◽  
Viable Cell Count ◽  
Proteus Vulgaris ◽  
Gastrointestinal Infections ◽  
Time Kill

The antibacterial activities of stem bark ethanolic extract ofErythrina caffraThunb. against bacteria in diarrhoea was determinedin vitroby the agar diffusion and dilution, macrobroth dilution, and time-kill assay methods. The result showed that the extract produced inhibition zones ranging between15±1.0 mm and23±1.0 mm, and the bacteria were susceptible at concentrations ranging between ≤100 and ≤1000 μg/mL. While the MICs of the extract ranged between 39.1 and 625 μg/mL, and the MBCs ranged between 78.1 and 625μg/mL, the MICs ofMicrococcus luteus,Proteus vulgarisCSIR 0030,Enterococcus faecalisKZN, andStaphylococcus aureusOK3were less than 100 μg/mL, and the mechanisms of antibiosis indicated that the crude ethanolic extract was highly bactericidal against the entire test bacteria isolates. In the time-kill assay, the averagelogreduction of the viable cell count ranged between0.916log 10and1.851log 10 cfu/mL on incubating the bacteria for 4 h at the MICs, while the reduction ranged between0.183log 10and1.105log 10 cfu/mL after 8 h of incubation. Incubating the bacteria for 4 h at 2 × MICs resulted in the reduction of the viable cell count to between −0.264log 10and0.961log 10 cfu/mL, while the averagelogreduction ranged between −3.968log 10and −0.425log 10 cfu/mL after 8 h of incubation withMicrococcus luteus,Proteus vulgarisCSIR 0030, andStaphylococcus aureusOK3being the most highly affected bacteria. The result showed that the extract exhibited broader-spectrum antibacterial activity and justifies the use ofErythrina caffrain the folkloric medicine for treating gastrointestinal infections in South Africa.

scholarly journals EVALUATION OF ANTICANCER ACTIVITY OF CUCUMIS CALLOSUS AGAINST EHRLICH’S ASCITES CARCINOMA BEARING MICE

2018 ◽  
Vol 11 (10) ◽  
pp. 438
Author(s):  
Siva Prasad Panda ◽  
Rajsekhar Reddy A ◽  
Uttam Prasad Panigrahy
Keyword(s):  
Cell Count ◽  
Anticancer Activity ◽  
Tumor Volume ◽  
Viable Cell ◽  
Viable Cell Count ◽  
Standard Drug ◽  
Tumor Weight ◽  
Eac Cells

Objective: Our previous research isolated Cucurbitacin B (CuB) and ebenone leucopentaacetate (ELP) from methanolic fruit extract of Cucumis callosus (MFCC). The fruits of C. callosus (Rottl.) Cogn. (Family: Cucurbitaceae) plant have been traditionally used for antioxidant, anti-inflammatory, and antidiabetic actions. The objective of this research was to evaluate in vitro and in vivo anticancer effect of MFCC on Ehrlich Ascites Carcinoma (EAC) cell lines.Methods: In vitro anticancer assay of MFCC and standard drug, 5-fluorouracil (5-FU) was evaluated using Trypan blue and 3-(4, 5-dimethylthiazol-yl)-2, 5-diphenyl tetrazolium bromide methods. In vivo anticancer activity of MFCC and 5-FU was also performed after 24h of EAC cells (2×106cells/ mouse) inoculation based on toxicity study for 9 consecutive days. The activity of the extract was assessed by the study of tumor volume, tumor weight, viable and non-viable cell count, hematological parameters, and biochemical estimations.Results: The MFCC showed the direct antitumor effect on EAC cells in a dose-dependent manner with an IG50 value of 0.61 mg/ml. Furthermore, MFCC (350 mg/kg) exhibited significant (p<0.01) decrease in tumor volume, tumor weight, and viable cell count of EAC-treated mice. Hematological profile, biochemical estimation assay significantly (p<0.01) reverted to normal level in MFCC, and 5-FU treated mice.Conclusion: The anticancer activity of fruits of C callosus is may be either due to the presence of CuB or/and ELP as phytoconstituent and the activity is comparable to standard drug 5-FU.

scholarly journals Antimicrobial screening and hemolytic activity of products obtained from Piper montealegreanum Yuncker and effect in vitro on growth of Staphylococcus aureus

2020 ◽  
Vol 9 (9) ◽  
pp. e346997410
Author(s):  
Wilma Raianny Vieira da Rocha ◽  
Luanne Eugenia Nunes ◽  
Anna Flávia Costa Fernandes ◽  
Raïssa Mayer Ramalho Catão ◽  
Harley da Silva Alves
Keyword(s):  
Staphylococcus Aureus ◽  
Hemolytic Activity ◽  
Membrane Damage ◽  
Clinical Importance ◽  
Ethanolic Extract ◽  
Antimicrobial Screening ◽  
Staphyloccocus Aureus ◽  
Time Kill ◽  
Chcl3 Fraction

This study aimed to determine spectrum of antimicrobial activity of crude ethanolic extract (CEE), chloroform (CHCl3) and ethyl acetate (EtOAc) fractions, to determine influence of CHCl3 fraction on Staphylococcus aureus growth and hemolytic activity. Antimicrobial screening of CEE, CHCl3 and EtOAc fractions was carried out using broth microdilution technique against standard strains: Staphyloccocus aureus ATCC25923, Escherichia coli ATCC25922, Pseudomonas aeruginosa ATCC27853 and Candida albicans ATCC76645 to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Time kill curves were performed to determine influence of CHCl3 fraction on MIC and ½ MIC concentrations on S. aureus growth. Hemolytic activity of CHCl3 was performed in human erythrocytes to report the cytotoxic effect. CHCl3 fraction showed activity against S. aureus with MIC=1024 µg mL-1 and MBC=2048 µg mL-1. This fraction did not show activity against other tested strains, as well as the CEE and EtOAc fraction. Time kill curve of S. aureus treated with CHCl3 fraction showed that at MIC this fraction has bacteriostatic activity, which was not observed with the ½ MIC. Hemolytic activity demonstrated that when CHCl3 fraction in MIC does not present membrane damage of erythrocytes, since it did not cause hemolysis. Studies should be conducted to evaluate the activity of CHCl3 fraction against S. aureus with different antimicrobial resistance profiles, in addition to evaluating the potential for resistance reversion by in vitro combination studies. The study demonstrated that the species P. montealegreanum can be an important source of compounds of importance for combating microorganisms of clinical importance.

scholarly journals Plants used in Bandjoun village (La'Djo) to cure infectious diseases: An ethnopharmacology survey and in-vitro TimeKill Assessment of some of them against Escherichia coli

2016 ◽  
Vol 5 (2) ◽  
pp. 56-70
Author(s):  
S.P. Bouopda Tamo ◽  
◽  
S.H. Riwom Essama ◽  
F.X. Etoa ◽  
◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Medicinal Plants ◽  
Infectious Diseases ◽  
Cell Count ◽  
Plant Extracts ◽  
Viable Cell ◽  
Viable Cell Count ◽  
Minimal Bactericidal Concentration ◽  
Log Reduction

An ethnopharmacology survey concerning the medicinal plants used in Bandjoun village (La'Djo) to cure infectious diseases was carried out in three districts of this village. The survey led to the identification of 79 medicinal plants species listed in 41 families. These plants were cited to be use to treat about 25 infectious diseases among which malaria, diarrhea and intestinal-worms were the most cited. Chromolaena odorata, Voacanga africana, Moringa oleifera, Mammea africana, Euphorbia hirta, Psidium guajava, Allium cepa, Enantia chlorantha, Alstonia boonei and Picralima nitida, were the ten most cited plants. Extractions of parts of these last plants were performed in hydro-ethanol (3:7) solvent and then tested in-vitro against an Escherichia coli isolate. The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were assessed by microdilution assay and the time-kill assessment was carried out by measure of log reduction in viable cell count, on a period of 48 hours. MIC and MBC determined were ranged between 1.00 and 32.0 mg/mL. Eighty percent (80%) of plant extracts tested have been bactericidal (MBC/MIC = 1 or 2) after 24 hours of incubation. A significant dose-dependent decreasing (P<0.05) in test organisms population was observed in the time with log reduction in viable cell count was ranged between 0.13 log10cfu/mL and 100% of inhibition. This antimicrobial activity has been attributed to metabolites groups in plant extracts namely, Phenols, flavonoids, tannins, coumarins, terpenoids, anthraquinones, cardiac glycosides, anthocyanides and alkaloids. These results obtained against Escherichia coli give a scientific validation to the traditional medical knowledge of Bandjoun-village populations and confirm some of the plants identified like a source of potentially active compounds against infectious diseases.

scholarly journals The influence of He-Ne Laser (632.8 nm) on Candida albicans isolated from complete upper dentures: In vitro study

2009 ◽  
Vol 6 (4) ◽  
pp. 790-796
Author(s):  
Baghdad Science Journal
Keyword(s):  
Candida Albicans ◽  
Low Power ◽  
Cell Viability ◽  
Cell Count ◽  
In Vitro Study ◽  
Viable Cell ◽  
Vitro Study ◽  
Viable Cell Count ◽  
Group I

The main objective of this study is to determine whether the use of He-Ne Laser (632.8 nm wavelength and power 0.5 mW) is an eligible and effective tool to kill or reduce the cell viability of Candida albicans isolated from complete upper dentures. Twenty one swabs were taken from the complete upper dentures. Only six swabs showed positive cultures for C. albicans. The isolate was divided into two groups, group I was not irradiated (control), and group II was irradiated by He-Ne Laser for different periods (10, 15, 20, and 30) min. After irradiation, the results showed a significant reduction in the viable cell count and colonies diameters especially at exposure periods 10 and 15 min. Although the low power He-Ne Laser was not eradicating the cell itself, but it affected on the viable cell count and colonies diameters comparing with the control.

scholarly journals Galvanic current dosage and bacterial concentration are determinants of the bactericidal effect of percutaneous needle electrolysis: an in vitro study

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
José Antonio García-Vidal ◽  
Jesús Salinas ◽  
Pilar Escolar-Reina ◽  
Francisco Cuello ◽  
Nieves Ortega ◽  
...  
Keyword(s):  
Cell Count ◽  
Saline Solution ◽  
In Vitro Study ◽  
Viable Cell ◽  
Bactericidal Effect ◽  
Viable Cell Count ◽  
High Concentration ◽  
Bacterial Concentration ◽  
Galvanic Current

AbstractPercutaneous needle electrolysis (PNE) is a physiotherapy technique that has been shown to be effective in different pathologies such as tendinopathies or mammary fistula. For many years, theoretical bactericidal and germicidal effects have been attributed to this type of galvanic currents, partly explained by the changes in pH that it generates. However, these effects have not yet been demonstrated. The aim of this study was to evaluate the bactericidal effect and the changes in pH caused by PNE. S. aureus were prepared in two different solutions (TSB and saline solution) and in different concentrations (from 9 to 6 Log10 CFU/mL). Bacteria were treated with three experimental PNE doses to assess bacterial death levels and the changes caused to the pH of the medium. The viable cell count showed that all experimental PNE doses had a bactericidal effect against a high concentration (9 Log10 CFU/mL) of S. aureus in saline solution (p < 0.001). Furthermore, we found that when the concentration of bacteria decreased, a lower dose of galvanic current generated the same effect as a higher dose. Changes in pH were registered only in experiments performed with saline solution. PNE had a bactericidal effect against S. aureus and the level of this effect was mainly modulated by the solution, the bacterial concentration and the dose. Changes affecting pH were modulated by the type of solution and there was no relationship between this and bacterial death.

In-Vitro Studies on the Sensitivity of Staphylococcus aureus to Some Ethno-Medicinal Preparations Sold around Kano, Nigeria

2011 ◽  
Vol 4 (1) ◽  
pp. 22-25 ◽  
Author(s):  
M Bashir ◽  
I Yusuf ◽  
AS Kutama
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Test Organism ◽  
Ethanolic Extract ◽  
Screening Tests ◽  
Phytochemical Screening ◽  
Herbal Preparations

Five traditional herbal preparations were sampled between May-June, 2009 in Kano. The samples were investigated for invitro antibacterial activities against clinical isolates of Staphylococcus aureus. Likewise, phytochemical screening tests were conducted to determine some of the phytochemicals present in the ethanolic and water extracts of the samples. Various concentrations of the extracts were prepared using serial doubling dilutions (5000=l/ml, 2500=g/ml, 1250=g/ml, 625=g/ml and 312.5=g/ml). All the test extracts showed slight antibacterial activity against the test organism, with ethanolic extract of sample E having the highest zone diameter of inhibition, while sample H had the lowest diameter of inhibition. The standard antibiotic disc (Gentamicin) had demonstrated the highest activity on the test organisms. The results of the Phytochemical screening revealed the presence of steroid in all the samples, tannin in samples A, C, D and E, reducing sugars in sample A, D and E respectively. The result of the minimum inhibitory concentration (MIC) was found to be above 312.5=g/ml for samples C, D and E. Keywords: Staphylococcus aureus, Herbal preparations, antibacterial activity, Phytochemical screening and minimum inhibitory concentration.

scholarly journals In Vitro Killing of Community-Associated Methicillin-Resistant Staphylococcus aureus with Drug Combinations

2004 ◽  
Vol 48 (10) ◽  
pp. 4016-4019 ◽  
Author(s):  
Samuel A. Shelburne ◽  
Daniel M. Musher ◽  
Kristina Hulten ◽  
Heather Ceasar ◽  
Michael Y. Lu ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Drug Combinations ◽  
Methicillin Resistant ◽  
Common Drug ◽  
Time Kill ◽  
Hospital Acquired

ABSTRACT This study employs time-kill techniques to examine the most common drug combinations used in the therapy of methicillin-resistant Staphylococcus aureus (MRSA) infections, vancomycin plus either gentamicin or rifampin. Community-associated MRSA were more likely to be synergistically inhibited by combinations of vancomycin and gentamicin versus vancomycin alone compared to inhibition associated with hospital-acquired strains.

scholarly journals In vitro activities of oxazolidinone compounds U100592 and U100766 against Staphylococcus aureus and Staphylococcus epidermidis.

1996 ◽  
Vol 40 (3) ◽  
pp. 799-801 ◽  
Author(s):  
G W Kaatz ◽  
S M Seo
Keyword(s):  
Staphylococcus Aureus ◽  
Staphylococcus Epidermidis ◽  
Inhibitory Activity ◽  
Antimicrobial Agents ◽  
Methicillin Resistant ◽  
Clinical Strains ◽  
Time Kill

The new oxazolidinone antimicrobial agents U100592 and U100766 demonstrated good in vitro inhibitory activity against clinical strains of Staphylococcus aureus and Staphylococcus epidermidis regardless of methicillin susceptibility. Both agents appeared bacteriostatic by time-kill analysis. Stable resistance to low multiples of the MIC of either drug could be produced only in methicillin-resistant S. aureus.

Performance of Some Heat-Sensitive Differential Agars Prepared and Melted by Microwave Energy1

1988 ◽  
Vol 51 (7) ◽  
pp. 577-578 ◽  
Author(s):  
C. LIANG ◽  
D. Y. C. FUNG
Keyword(s):  
Escherichia Coli ◽  
Cell Count ◽  
Viable Cell ◽  
Microwave Energy ◽  
Microwave Oven ◽  
Viable Cell Count ◽  
Streptococcus Faecalis ◽  
Cell Counts ◽  
Significant Difference ◽  
Conventional Boiling

The viable cell count performance of some heat-sensitive differential agars prepared and remelted by microwave energy was evaluated for Salmonella choleraesui, Streptococcus faecalis and Escherichia coli. The conventional boiling method was used for comparison. No significant difference was found between the microwave oven processed agar and the conventional-boiling processed agar in viable cell counts of the target bacteria. Heating and reheating of violet red bile agar, bismuth sulfite agar, and KF Streptococcus agar by both methods did not change agar performance. However, remelting of desoxycholate citrate agar by both methods resulted in a substantial lowering of viable cell counts.

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