scholarly journals The Effect of High Concentrations of Glufosinate Ammonium on the Yield Components of Transgenic Spring Wheat (Triticum aestivumL.) Constitutively Expressing thebarGene

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Zoltán Áy ◽  
Róbert Mihály ◽  
Mátyás Cserháti ◽  
Éva Kótai ◽  
János Pauk
Keyword(s):  
Yield Components ◽  
Lethal Dose ◽  
Experimental System ◽  
Kernel Weight ◽  
Wheat Line ◽  
Glufosinate Ammonium ◽  
Kernel Weights ◽  
High Concentrations ◽  
Effective Component ◽  
Yield Depression

We present an experiment done on abar+wheat line treated with 14 different concentrations of glufosinate ammonium—an effective component of nonselective herbicides—during seed germination in a closed experimental system. Yield components as number of spikes per plant, number of grains per spike, thousand kernel weight, and yield per plant were thoroughly analysed and statistically evaluated after harvesting. We found that a concentration of glufosinate ammonium 5000 times the lethal dose was not enough to inhibit the germination of transgenic plants expressing thebargene. Extremely high concentrations of glufosinate ammonium caused a bushy phenotype, significantly lower numbers of grains per spike, and thousand kernel weights. Concerning the productivity, we observed that concentrations of glufosinate ammonium 64 times the lethal dose did not lead to yield depression. Our results draw attention to the possibilities implied in the transgenic approaches.

EFFECTS OF REDUCING INTERPLANT COMPETITION AT DIFFERENT STAGES OF GROWTH ON STALK ROT AND YIELD COMPONENTS OF CORN

1969 ◽  
Vol 49 (6) ◽  
pp. 723-729 ◽  
Author(s):  
C. G. Mortimore ◽  
L. F. Gates
Keyword(s):  
Yield Components ◽  
Kernel Weight ◽  
Yield Component ◽  
Maximum Effect ◽  
Physiological Maturity ◽  
Stalk Rot ◽  
Stress Effects ◽  
Stages Of Growth ◽  
Kernel Weights ◽  
Interplant Competition

Stress effects on stalk rot and yield components of corn (Zea mays L.), arising from interplant competition, were, studied by removing at various times either the competing plants or the developing ears. The number of ears per plant and the number of kernels per ear at harvest were greater when plants were thinned before mid-silk, but the only yield component associated with stalk rot was 1000-kernel weight.Removal of alternate plants at any time up to 4 weeks after mid-silk greatly reduced the proportion of plants developing stalk rot. In plots thinned less than 4 weeks before physiological maturity, the incidence of stalk rot increased progressively as thinning was delayed. Similarly, up to 1 month before physiological maturity, stress had little effect on weight of individual kernels but when the stress was continued into the month which preceded physiological maturity, individual kernel weights were substantially decreased. Ear removal 3 weeks or more before maturity practically eliminated stalk rot whereas later removal had progressively less effect. Thus, the deleterious effects of stress from interplant competition and ear development had their maximum effect on stalk rot susceptibility and kernel weight during the month before physiological maturity.

scholarly journals Epistasis for Three Grain Yield Components in Rice (Oryxa sativa L.)

Genetics ◽  
1997 ◽  
Vol 145 (2) ◽  
pp. 453-465 ◽  
Author(s):  
Zhikang Li ◽  
Shannon R M Pinson ◽  
William D Park ◽  
Andrew H Paterson ◽  
James W Stansel
Keyword(s):  
Grain Yield ◽  
Complex Traits ◽  
Yield Components ◽  
Genetic Basis ◽  
Kernel Weight ◽  
Progeny Testing ◽  
Grain Yield Components ◽  
Main Effects ◽  
Grain Number Per Panicle

The genetic basis for three grain yield components of rice, 1000 kernel weight (KW), grain number per panicle (GN), and grain weight per panicle (GWP), was investigated using restriction fragment length polymorphism markers and F4 progeny testing from a cross between rice subspecies japonica (cultivar Lemont from USA) and indica (cv. Teqing from China). Following identification of 19 QTL affecting these traits, we investigated the role of epistasis in genetic control of these phenotypes. Among 63 markers distributed throughout the genome that appeared to be involved in 79 highly significant (P < 0.001) interactions, most (46 or 73%) did not appear to have “main” effects on the relevant traits, but influenced the trait(s) predominantly through interactions. These results indicate that epistasis is an important genetic basis for complex traits such as yield components, especially traits of low heritability such as GN and GWP. The identification of epistatic loci is an important step toward resolution of discrepancies between quantitative trait loci mapping and classical genetic dogma, contributes to better understanding of the persistence of quantitative genetic variation in populations, and impels reconsideration of optimal mapping methodology and marker-assisted breeding strategies for improvement of complex traits.

Effect of nitrogen, seeding date and cultivar on oat quality and yield in the eastern Canadian prairies

2004 ◽  
Vol 84 (4) ◽  
pp. 1025-1036 ◽  
Author(s):  
William E. May ◽  
Ramona M. Mohr ◽  
Guy P. Lafond ◽  
Adrian M. Johnston ◽  
F. Craig Stevenson
Keyword(s):  
Grain Yield ◽  
Avena Sativa ◽  
Yield Components ◽  
Seed Quality ◽  
Kernel Weight ◽  
Yield Component ◽  
Human Consumption ◽  
Seeding Date ◽  
N Rates

The proportion of oat (Avena sativa L.) being used for race horses and human consumption has increased over the past 15 yr. The objective of this study was to evaluate the effects of N, seeding date and cultivar on grain yield components, grain yield and grain quality of oat under a direct seeding system. Four N rates, three seeding dates and two cultivars were tested at Indian Head, Melfort, and Canora, SK, and Brandon, MB. Yield was more responsive to increasing N rates from 15 and 80 kg ha-1 when oat was seeded in early May versus early June. Panicles plant-1 was the yield component that accounted for most of the yield increase achieved from increasing rates of N, while kernel weight was the yield component that decreased as the rate of N increased. Physical seed quality decreased (plump seed decreased and thin seed increased) with delayed seeding and greater N fertilizer rates. Nitrogen fertilizer and seeding date had a much larger effect on the quality of CDC Pacer than AC Assiniboia. Combining early seeding, appropriate N fertility and well-adapted cultivars should increase the likelihood of optimizing oat yield and quality necessary for high-value markets. Key words: Avena sativa L., yield components, test weight, lodging, plump seed, thin seed

scholarly journals Antioxidant Therapy in Parkinson’s Disease: Insights from Drosophila melanogaster

Antioxidants ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 52 ◽  
Author(s):  
Federica De Lazzari ◽  
Federica Sandrelli ◽  
Alexander J. Whitworth ◽  
Marco Bisaglia
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Drosophila Melanogaster ◽  
Therapeutic Potential ◽  
Experimental System ◽  
Rapid Screening ◽  
Antioxidant Compounds ◽  
Oxygen Species ◽  
Protective Effects ◽  
High Concentrations

Reactive oxygen species (ROS) play an important role as endogenous mediators in several cellular signalling pathways. However, at high concentrations they can also exert deleterious effects by reacting with many macromolecules including DNA, proteins and lipids. The precise balance between ROS production and their removal via numerous enzymatic and nonenzymatic molecules is of fundamental importance for cell survival. Accordingly, many neurodegenerative disorders, including Parkinson’s disease (PD), are associated with excessive levels of ROS, which induce oxidative damage. With the aim of coping with the progression of PD, antioxidant compounds are currently receiving increasing attention as potential co-adjuvant molecules in the treatment of these diseases, and many studies have been performed to evaluate the purported protective effects of several antioxidant molecules. In the present review, we present and discuss the relevance of the use of Drosophila melanogaster as an animal model with which to evaluate the therapeutic potential of natural and synthetic antioxidants. The conservation of most of the PD-related genes between humans and D. melanogaster, along with the animal’s rapid life cycle and the versatility of genetic tools, makes fruit flies an ideal experimental system for rapid screening of antioxidant-based treatments.

Creatine kinase BB: a new tumor-associated marker.

1979 ◽  
Vol 25 (8) ◽  
pp. 1432-1435 ◽  
Author(s):  
L M Silverman ◽  
G B Dermer ◽  
M H Zweig ◽  
A C Van Steirteghem ◽  
Z A Tökés
Keyword(s):  
Creatine Kinase ◽  
Epithelial Cells ◽  
Prostatic Carcinoma ◽  
Experimental System ◽  
Immunoperoxidase Staining ◽  
Prostatic Fluid ◽  
Agarose Electrophoresis ◽  
Adenocarcinoma Of The Prostate ◽  
High Concentrations ◽  
Malignant Prostate

Abstract Creatine kinase isoenzyme BB (CK-BB) is found in the serum of patients with various types of cancer. Using both radioimmunoassay and agarose electrophoresis, we found abnormal amounts of this isoenzyme in the serum of 15 of 17 patients with untreated prostatic carcinoma. Among patients with other types of adenocarcinomas and metastatic disease, serum CK-BB was increased in most of those who were unresponsive to therapy. In benign or malignant prostate tissue and in malignant pleural effusions, cytoplasmic CK-BB as determined by immunoperoxidase staining was found in epithelial cells. Prostatic fluid had high concentrations of CK-BB, as did malignant, but not benign, pleural fluid supernates. We conclude that glandular epithelial cells contain CK-BB, which is released in benign and malignant states and may appear in higher concentrations in the circulation in malignant states. These conclusions are consistent with predictions we have made from a model experimental system concerning characteristics of possible tumor markers. The observations indicate a role for CK-BB as a tumor marker, particularly for adenocarcinoma of the prostate.

scholarly journals Incredible Role of Osmotic Adjustment in Grain Yield Sustainability under Water Scarcity Conditions in Wheat (Triticum aestivum L.)

Plants ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1208
Author(s):  
Tahir Mahmood ◽  
Muhammad Abdullah ◽  
Sunny Ahmar ◽  
Muhammad Yasir ◽  
Muhammad Shahid Iqbal ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
Osmotic Adjustment ◽  
Yield Components ◽  
Positive Association ◽  
Kernel Weight ◽  
Environment Interaction ◽  
Plant Yield ◽  
Wheat Genotypes ◽  
Yield Sustainability

Interrogations of local germplasm and landraces can offer a foundation and genetic basis for drought tolerance in wheat. Potential of drought tolerance in a panel of 30 wheat genotypes including varieties, local landraces, and wild crosses were explored under drought stress (DS) and well-watered (WW) conditions. Considerable variation for an osmotic adjustment (OA) and yield components, coupled with genotype and environment interaction was observed, which indicates the differential potential of wheat genotypes under both conditions. Reduction in yield per plant (YP), thousand kernel weight (TKW), and induction of OA was detected. Correlation analysis revealed a strong positive association of YP with directly contributing yield components under both environments, indicating the impotence of these traits as a selection-criteria for the screening of drought-tolerant genotypes for drylands worldwide. Subsequently, the association of OA with TKW which contributes directly to YP, indicates that wheat attains OA to extract more water from the soil under low water-potential. Genotypes including WC-4, WC-8 and LLR-29 showed more TKW under both conditions, among them; LLR-29 also has maximum OA and batter yield comparatively. Result provides insight into the role of OA in plant yield sustainability under DS. In this study, we figure out the concept of OA and its incredible role in sustainable plant yield in wheat.

scholarly journals Zinc and amino acids impact on nutrient status of maize during the ‘critical window’

2018 ◽  
Vol 64 (No. 3) ◽  
pp. 126-131
Author(s):  
Szczepaniak Witold ◽  
Potarzycki Jarosław ◽  
Grzebisz Witold ◽  
Nowicki Bartłomiej
Keyword(s):  
Amino Acids ◽  
Nutritional Status ◽  
Nutrient Status ◽  
Kernel Weight ◽  
Simultaneous Application ◽  
Critical Window ◽  
Combined Application ◽  
Beneficial Impact ◽  
Yield Depression ◽  
Yield Decrease

It has been assumed that zinc (Zn) fertilizers applied to maize simultaneously with amino acids (AA) at early stages of its growth may decrease the yield variability due to correcting its nutritional status during the ‘critical window’. Two Zn carriers were evaluated (Zn-I – Zn chelate; Zn-II – Zn oxide); they were applied to maize at BBCH 14/15 with or without amino acids, based on two rates of nitrogen (80 and 160 kg N/ha). The precipitation deficiency in 2015 resulted in the grain yield decrease by 35% compared to 2014. An advantage of higher N rate was proved in 2014, whereas the influence of Zn and AA showed in 2015. In this year, the beneficial impact of Zn-oxide and AA combined application resulted in amelioration, at least partially, of the imbalance of certain macronutrient content (N, P, Mg) during the ‘critical window’. These effects were revealed due to a boosted number of kernels in cob, and particularly higher thousand kernel weight. Consequently, the yield depression in 2015 was partly overcome. The results indicated that simultaneous application of Zn oxide and AA to maize at BBCH 14/15 corrected both its nutritional status during the ‘critical window’ and yield components, but had no effect on the yield itself.

scholarly journals Influence of Vancomycin Infusion Methods on Endothelial Cell Toxicity

2014 ◽  
Vol 59 (2) ◽  
pp. 930-934 ◽  
Author(s):  
Maryline Drouet ◽  
Feng Chai ◽  
Christine Barthélémy ◽  
Gilles Lebuffe ◽  
Bertrand Debaene ◽  
...  
Keyword(s):  
Continuous Infusion ◽  
Umbilical Vein ◽  
Lethal Dose ◽  
Intermittent Infusion ◽  
Common Side Effect ◽  
Cell Toxicity ◽  
Intravenous Therapy ◽  
High Concentrations ◽  
Umbilical Vein Endothelial Cells

ABSTRACTPeripheral intravenous therapy is frequently used in routine hospital practice and, due to various factors, its most common side effect is phlebitis. The infusion of vancomycin is particularly associated with phlebitis despite its widespread use. French guidelines recommend central intravenous infusion for high concentrations of vancomycin, but peripheral intravenous therapy is often preferred in intensive care units. Methods of vancomycin infusion are either intermittent infusion or continuous infusion. A comparison of these methods underin vitroconditions simulating clinical use could result in better infusion efficacy. Human umbilical vein endothelial cells (HUVECs) were therefore challenged with clinical doses of vancomycin over a 24- to 72-h period using these infusion methods. Cell death was measured with the alamarBlue test. Concentration-dependent and time-dependent vancomycin toxicity on HUVECs was noted with a 50% lethal dose at 5 mg/ml after 24 h, reaching 2.5 mg/ml after 72 h of infusion, simulating long-term infusion. This toxicity does not seem to be induced by acidic pH. In comparing infusion methods, we observed that continuous infusion induced greater cell toxicity than intermittent infusion at doses higher than 1 g/day. The increasing use of vancomycin means that new guidelines are required to avoid phlebitis. If peripheral intravenous therapy is used to reduce infusion time, along with intermittent infusion, vein irritation and localized phlebitis may be reduced. Further studies have to be carried out to explore the causes of vancomycin endothelial toxicity.

scholarly journals Electroporation can cause artefacts due to solubilization of cations from the electrode plates. Aluminum ions enhance conversion of inositol 1,3,4,5-tetrakisphosphate into inositol 1,4,5-trisphosphate in electroporated L1210 cells

1991 ◽  
Vol 277 (3) ◽  
pp. 883-885 ◽  
Author(s):  
J W Loomis-Husselbee ◽  
P J Cullen ◽  
R F Irvine ◽  
A P Dawson
Keyword(s):  
Experimental System ◽  
Experimental Medium ◽  
Assay Medium ◽  
L1210 Cells ◽  
Aluminum Ions ◽  
Inositol 1,4,5 Trisphosphate ◽  
The Difference ◽  
High Concentrations ◽  
As If ◽  
In Cells

1. In electroporated L1210 cells, Ins(1,3,4,5)P4 causes Ca2+ release, owing to its conversion into Ins(1,4,5)P3, but this does not happen in cells permeabilized by digitonin treatment [Cullen, Irvine, Drøbak & Dawson (1989) Biochem. J. 259, 931-933]. 2. If the assay medium is subjected to electroporation by using a commercially available electroporation apparatus and then the cells are added and permeabilized with digitonin, the cells behave as if they had been electroporated. 3. Electroporation causes the release of high concentrations of Al3+ into the experimental medium, and addition of these concentrations of Al3+ into the experimental medium mimics the effect of electroporation on the conversion of Ins(1,3,4,5)P4 into Ins(1,4,5)P3. 4. It is concluded that the difference between electroporated and digitonin-permeabilized L1210 cells in this experimental system can be attributed to dissolution of Al3+ from the electroporation cuvette. Al3+ contamination may thus be a serious problem when using this apparatus.

scholarly journals Genome wide identification of QTL associated with yield and yield components in two popular wheat cultivars TAM 111 and TAM 112

2020 ◽  
Author(s):  
Yan Yang ◽  
Smit Dhakal ◽  
Chenggen Chu ◽  
Shichen Wang ◽  
Qingwu Xue ◽  
...  
Keyword(s):  
Grain Yield ◽  
Great Plains ◽  
Yield Components ◽  
Recombinant Inbred Lines ◽  
Wheat Breeding ◽  
Kernel Weight ◽  
Genetic Maps ◽  
Wheat Cultivars ◽  
Thousand Kernel Weight ◽  
Yield And Yield Components

AbstractTwo drought-tolerant wheat cultivars, ‘TAM 111’ and ‘TAM 112’, have been widely grown in the Southern Great Plains of the U.S. and used as parents in many wheat breeding programs worldwide. This study aimed to reveal genetic control of yield and yield components in the two cultivars under both dryland and irrigated conditions. A mapping population containing 124 F5:7 recombinant inbred lines (RILs) was developed from the cross of TAM 112/TAM 111. A set of 5,948 SNPs from the wheat 90K iSelect array and double digest restriction-site associated DNA sequencing was used to construct high-density genetic maps. Data for yield and yield components were obtained from 11 environments. QTL analyses were performed based on 11 individual environments, across all environments, within and across mega-environments. Thirty-six unique consistent QTL regions were distributed on 13 chromosomes including 1A, 1B, 1D, 2A, 2D, 3D, 4B, 4D, 6A, 6B, 6D, 7B, and 7D. Ten unique QTL with pleiotropic effects were identified on four chromosomes and eight were in common with the consistent QTL. These QTL increased dry biomass grain yield by 16.3 g m−2, plot yield by 28.1 g m−2, kernels spike−1 by 0.7, spikes m−2 by 14.8, thousand kernel weight by 0.9 g with favorable alleles from either parent. TAM 112 alleles mainly increased spikes m−2 and thousand kernel weight while TMA 111 alleles increased kernels spike−1, harvest index and grain yield. The saturated genetic map and markers linked to significant QTL from this study will be very useful in developing high throughput genotyping markers for tracking the desirable haplotypes of these important yield-related traits in popular parental cultivars.

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