scholarly journals mRNA Expression of EgCHI1, EgCHI2, and EgCHI3 in Oil Palm Leaves (Elaeis guineesisJacq.) after Treatment withGanoderma boninensePat. andTrichoderma harzianumRifai

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Laila Naher ◽  
Soon Guan Tan ◽  
Chai Ling Ho ◽  
Umi Kalsom Yusuf ◽  
Siti Hazar Ahmad ◽  
...  
Keyword(s):  
Oil Palm ◽  
Biocontrol Agent ◽  
Elaeis Guineensis ◽  
Early Stage ◽  
Stem Rot ◽  
Ganoderma Boninense ◽  
Early Disease ◽  
Basal Stem Rot ◽  
Early Disease Detection ◽  
Serious Disease

Background. Basal stem rot (BSR) disease caused by the fungusGanoderma boninenseis the most serious disease affecting the oil palm; this is because the disease escapes the early disease detection. The biocontrol agentTrichoderma harzianumcan protect the disease only at the early stage of the disease. In the present study, the expression levels of three oil palm (Elaeis guineensisJacq.) chitinases encoding EgCHI1, EgCHI2, and EgCHI3 at 2, 5, and 8 weeks inoculation were measured in oil palm leaves from plants treated withG. boninenseorT. harzianumalone or both.Methods. The five-month-old oil palm seedlings were treated with Gano-wood blocks inoculum and trichomulch. Expression of EgCHI1, EgCHI2, and EgCHI3 in treated leaves tissue was determined by real-time PCR.Results. Oil palm chitinases were not strongly expressed in oil palm leaves of plants treated withG. boninensealone compared to other treatments. Throughout the 8-week experiment, expression of EgCHI1 increased more than 3-fold in leaves of plants treated withT. harzianumandG. boninensewhen compared to those of control and other treated plants.Conclusion. The data illustrated that chitinase cDNA expression varied depending on tissue and the type of treatment.

Mycoparasitic Scytalidium parasiticum as a potential biocontrol agent against Ganoderma boninense basal stem rot in oil palm

2016 ◽  
Vol 26 (10) ◽  
pp. 1352-1365 ◽  
Author(s):  
Yit Kheng Goh ◽  
Nurul Fadhilah Marzuki ◽  
Teik Khiang Goh ◽  
Suet Yee Tan ◽  
You Keng Goh ◽  
...  
Keyword(s):  
Oil Palm ◽  
Biocontrol Agent ◽  
Stem Rot ◽  
Ganoderma Boninense ◽  
Basal Stem Rot ◽  
Potential Biocontrol Agent

scholarly journals EFFECT OF MICRONUTRIENTS-ENRICHED FERTILIZERS ON BASAL STEM ROT DISEASE INCIDENCE AND SEVERITY ON OIL PALM (ELAEIS GUINEENSIS JACQ.) SEEDLINGS

2014 ◽  
Vol 11 (10) ◽  
pp. 1841-1859 ◽  
Author(s):  
Fabien Fonguimgo Tengoua ◽  
Mohamed M. Hanafi ◽  
A. S. Idris ◽  
Kadir Jugah ◽  
Jamaludin Nurul Mayziatul Azwa ◽  
...  
Keyword(s):  
Oil Palm ◽  
Elaeis Guineensis ◽  
Disease Incidence ◽  
Stem Rot ◽  
Basal Stem Rot ◽  
Rot Disease

scholarly journals Antioxidant Enzyme Activities and Secondary Metabolite Profiling of Oil Palm Seedlings Treated with Combination of NPK Fertilizers Infected with Ganoderma boninense

2018 ◽  
Vol 2018 ◽  
pp. 1-18
Author(s):  
Mahbod Sahebi ◽  
Mohamed M. Hanafi ◽  
Hasmah Mohidin ◽  
M. Y. Rafii ◽  
Parisa Azizi ◽  
...  
Keyword(s):  
Secondary Metabolite ◽  
Oil Palm ◽  
Defense Mechanism ◽  
Elaeis Guineensis ◽  
Growth And Yield ◽  
Stem Rot ◽  
Antioxidant Enzyme Activities ◽  
Npk Fertilizers ◽  
Basal Stem Rot ◽  
Cell Defense

Oil palm (Elaeis guineensis Jacq) is one of the major sources of edible oil. Reducing the effect of Ganoderma, main cause of basal stem rot (BSR) on oil palm, is the main propose of this study. Understanding the oil palm defense mechanism against Ganoderma infection through monitoring changes in the secondary metabolite compounds levels before/after infection by Ganoderma under different fertilizing treatment is required. Oil palm requires macro- and microelements for growth and yield. Manipulating the nutrient for oil palm is a method to control the disease. The 3-4-month-old oil palm seedlings were given different macronutrient treatments to evaluate induction of defense related enzymes and production of secondary metabolite compounds in response to G. boninense inoculation. The observed trend of changes in the infected and uninfected seedlings was a slightly higher activity for β-1,3-glucanases, chitinase, peroxidase, and phenylalanine ammonia-lyase during the process of pathogenesis. It was found that PR proteins gave positive response to the interaction between oil palm seedlings and Ganoderma infection. Although the responses were activated systematically, they were short-lasting as the changes in enzymes activities appeared before the occurrence of visible symptoms. Effect of different nutrients doses was obviously observed among the results of the secondary metabolite compounds. Many identified/unidentified metabolite compounds were presented, of which some were involved in plant cell defense mechanism against pathogens, mostly belonging to alkaloids with bitter-tasting nitrogenous-compounds, and some had the potential to be used as new markers to detect basal stem rot at the initial step of disease.

scholarly journals RNA-seq data of Ganoderma boninense at axenic culture condition and under in planta pathogen-oil palm (Elaeis guineensis Jacq.) interaction

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Mui Yun Wong ◽  
Nisha T. Govender ◽  
Chia Sui Ong
Keyword(s):  
Oil Palm ◽  
Molecular Mechanisms ◽  
Elaeis Guineensis ◽  
Early Stage ◽  
Axenic Culture ◽  
Economic Losses ◽  
Post Inoculation ◽  
Ganoderma Boninense ◽  
In Planta ◽  
Rot Disease

Abstract Objective Basal stem rot disease causes severe economic losses to oil palm production in South-east Asia and little is known on the pathogenicity of the pathogen, the basidiomyceteous Ganoderma boninense. Our data presented here aims to identify both the house-keeping and pathogenicity genes of G. boninense using Illumina sequencing reads. Description The hemibiotroph G. boninense establishes via root contact during early stage of colonization and subsequently kills the host tissue as the disease progresses. Information on the pathogenicity factors/genes that causes BSR remain poorly understood. In addition, the molecular expressions corresponding to G. boninense growth and pathogenicity are not reported. Here, six transcriptome datasets of G. boninense from two contrasting conditions (three biological replicates per condition) are presented. The first datasets, collected from a 7-day-old axenic condition provide an insight onto genes responsible for sustenance, growth and development of G. boninense while datasets of the infecting G. boninense collected from oil palm-G. boninense pathosystem (in planta condition) at 1 month post-inoculation offer a comprehensive avenue to understand G. boninense pathogenesis and infection especially in regard to molecular mechanisms and pathways. Raw sequences deposited in Sequence Read Archive (SRA) are available at NCBI SRA portal with PRJNA514399, bioproject ID.

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