scholarly journals Pregnancy Rates with Recombinant versus Urinary Human Chorionic Gonadotropin in In Vitro Fertilization: An Observational Study

2011 ◽  
Vol 11 ◽  
pp. 1781-1787 ◽  
Author(s):  
József Zeke ◽  
Katalin Kanyó ◽  
Helga Zeke ◽  
Áron Cseh ◽  
Barna Vásárhelyi ◽  
...  
Keyword(s):  
Clinical Trials ◽  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Clinical Characteristics ◽  
Randomized Clinical Trials ◽  
Real Life ◽  
In Vitro Fertilisation ◽  
Vitro Fertilization ◽  
Equal Efficacy

Randomized clinical trials (RCTs) demonstrated the equal efficacy of urinary human chorionic gonadotropin (uhCG) and recombinant hCG (rhCG) products in in vitro fertilisation (IVF). However, limitations inherent with RCTs necessitate the reinforcement of RCT results in real-life. We retrospectively analyzed pregnancies after treatment with rhCG and uhCG products (n=391, and 96, resp.). We found that laboratory-verified pregnancy occurred more frequently in rhCG patients than in those on uhCG (43% versus 30%,P=0.02). The association remains significant (P=0.002) after its adjustment for clinical characteristics. The prevalence of laboratory-verified pregnancies was higher with GnRH agonist use (P=0.012) and BMI under 30 kg/m2(P=0.053) while decreased the age (P=0.014) and the number of previous failed attempts (P=0.08). Similar (but not significant) trends were observed with rates of pregnancy filled the 24th week. These results reinforce RCTs supporting the notion that rhCG is more efficient as uhCG during IVF.

scholarly journals Evaluation of Nicked Human Chorionic Gonadotropin Content in Clinical Specimens by a Specific Immunometric Assay

1999 ◽  
Vol 45 (1) ◽  
pp. 68-77 ◽  
Author(s):  
Galina Kovalevskaya ◽  
Steven Birken ◽  
Tatsu Kakuma ◽  
John Schlatterer ◽  
John F O’Connor
Keyword(s):  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Normal Pregnancy ◽  
Physical Separation ◽  
Vitro Fertilization ◽  
Clinical Specimens ◽  
Reference Preparation ◽  
Ectopic Pregnancies

Abstract We report the development and characterization of an IRMA for the direct measurement of nicked human chorionic gonadotropin (hCGn) in blood and urine. hCGn derived from a reference preparation of hCG used as an immunogen elicits monoclonal antibodies (mAbs) with enhanced recognition of human luteinizing hormone epitopes. The most specific assay for pregnancy hCGn is an IRMA composed of one mAb to choriocarcinoma-derived hCGn (C5) and a second mAb developed from immunization with normal-pregnancy hCGn. This assay was used to evaluate hCGn profiles in normal, in vitro fertilization, Down syndrome, and ectopic pregnancies. In all pregnancies, hCGn was usually present in much lower concentrations than the non-nicked hCG isoform. Our results suggest that some form of physical separation from the overwhelming quantities of non-nicked hCG present in clinical specimens will be required before accurate immunochemical estimations of hCGn can be made.

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