A novel, fast-growing Borrelia sp. isolated from the hard tick Hyalomma aegyptium in Turkey

Microbiology ◽  
2003 ◽  
Vol 149 (9) ◽  
pp. 2539-2544 ◽  
Author(s):  
Ece S. Güner ◽  
Naoya Hashimoto ◽  
Teruki Kadosaka ◽  
Yasuyuki Imai ◽  
Toshiyuki Masuzawa
Keyword(s):  
Sequence Similarity ◽  
Optimal Growth ◽  
Hard Tick ◽  
The Novel ◽  
Borrelia Hermsii ◽  
Fast Growing ◽  
Sds Page ◽  
Northwestern Turkey ◽  
Observation Sequence ◽  
Pure Cultures

A novel, fast-growing spirochaete was isolated from the hard tick Hyalomma aegyptium (family Ixodidae, subfamily Metastriata) using Barbour–Stoenner–Kelly (BSK) II medium. Tick samples were taken during the summer of 2000 from the Istanbul area in northwestern Turkey. Sixty-seven of 153 adults (44 %) and 72 of 185 nymphs (39 %) were infected with the novel spirochaete, whereas none of the 20 larvae examined were infected. The optimal growth temperature of the spirochaete in BSK II medium was 34–37 °C, and it could grow at 39 °C. Doubling times at 34 and 37 °C were 5·3 and 5·1 h, respectively. Six pure cultures of the spirochaete were obtained and characterized by microscopic observation, sequence analysis of the flagellin gene (flaB), SDS-PAGE and Western blotting. The spirochaete was morphologically similar to those of the genus Borrelia and contained a 41 kDa protein reactive with mAb H9724 specific to the flagellin of a Borrelia species. Polyclonal antibody raised to this spirochaete reacted with several antigen bands, whereas no bands were detected with Borrelia burgdorferi, Borrelia hermsii, Borrelia turicatae and Borrelia parkeri. The flaB sequences of the six isolates showed high similarity, with sequence similarity values ranging from 99·2 to 100 %; however, the similarity of the isolates' flaB sequences to those of the Lyme-disease-related Borrelia and relapsing-fever-associated Borrelia species was less than 90 %. These findings suggest that the unique spirochaete is a member of the genus Borrelia, and differs from previously described Borrelia species.

scholarly journals Sphingomonas hengshuiensis sp. nov., isolated from lake wetland

2015 ◽  
Vol 65 (Pt_12) ◽  
pp. 4644-4649 ◽  
Author(s):  
Shuzhen Wei ◽  
Tingting Wang ◽  
Hongliang Liu ◽  
Caifeng Zhang ◽  
Jiping Guo ◽  
...  
Keyword(s):  
Sequence Similarity ◽  
Optimal Growth ◽  
Northern China ◽  
Rrna Gene ◽  
The Novel ◽  
Respiratory Quinone ◽  
Closely Related Species ◽  
Novel Bacterium ◽  
The Status ◽  
Type Strains

A polyphasic taxonomic study was undertaken to establish the status of a novel bacterium, designated strain WHSC-8T, which was isolated from soil of Hengshui Lake Wetland Reserve in Hebei province, northern China. Colonies of this strain were yellow and cells were rod-shaped, polar-flagellated and obligately aerobic, exhibiting negative Gram reaction. The strain was able to grow at 0–1 % (w/v) NaCl, pH 5–10 and 20–35 °C, with optimal growth occurring at pH 7.0 and 28 °C without NaCl. Chemotaxonomic data revealed that strain WHSC-8T possesses ubiquinone Q-10 as the predominant respiratory quinone, C18 : 1ω7c, C16 : 0 and summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH) as the major fatty acids, and sym-homospermidine as the major polyamine. Sphingomonadaceae-specific sphingoglycolipid was detected in the polar lipid patterns. The G+C content of the genomic DNA was 68.7 mol%. All of the above characters corroborated the assignment of the novel strain to the genus Sphingomonas. Strain WHSC-8T shared less than 97.0 % 16S rRNA gene sequence similarity with the type strains of other species of the genus Sphingomonas, except for Sphingomonas asaccharolytica DSM 10564T (97.5 %). The low DNA–DNA relatedness value and distinct phenotypic and chemotaxonomic characteristics distinguished strain WHSC-8T from closely related species of the genus Sphingomonas. Therefore, strain WHSC-8T represents a novel species of the genus Sphingomonas, for which the name Sphingomonas hengshuiensis sp. nov. is proposed. The type strain is WHSC-8T ( = KCTC 42455T = CCTCC AB 2015265T).

scholarly journals Idiomarina aquatica sp. nov., a moderately halophilic bacterium isolated from salterns

2015 ◽  
Vol 65 (Pt_12) ◽  
pp. 4595-4600 ◽  
Author(s):  
María José León ◽  
Fernando Martínez-Checa ◽  
Antonio Ventosa ◽  
Cristina Sánchez-Porro
Keyword(s):  
Novel Species ◽  
Sequence Similarity ◽  
Optimal Growth ◽  
Halophilic Bacterium ◽  
Rrna Gene ◽  
The Novel ◽  
Bacterial Strains ◽  
Moderately Halophilic Bacterium ◽  
Curved Rods ◽  
Content Range

Four bacterial strains, SN-14T, SN-4, M6-46 and M6-58B, were isolated from water of ponds of two salterns located in Huelva (Spain). They were Gram-stain-negative, aerobic and slightly curved rods. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the four strains belong to the genus Idiomarina, being related most closely to Idiomarina fontislapidosi F23T (98.4–98.0% sequence similarity), Idiomarina seosinensis CL-SP19T (98.3–98.0%), Idiomarina piscisalsi TPS4-2T (97.9–97.4%), Idiomarina baltica OS145T (97.5–97.4%) and Idiomarina zobellii KMM 231T (97.6–97.0%). The level of similarity with the type species of the genus, Idiomarina abyssalis KMM 227T, was 97.2–96.7%. The novel strains exhibited optimal growth at 5–10% (w/v) total salts, pH 7 and 37 °C. The major fatty acids of strain SN-14T were iso-C15 : 0, iso-C17 : 0, C18 : 1ω7c/C18 : 1ω6c, C16 : 0 and iso-C17 : 1ω9c/C16 : 0 10-methyl. The DNA G+C content range was 47.6–50.8 mol%. The level of DNA–DNA relatedness between strain SN-14T and I. fontislapidosi F23T was 13%, while those between strain SN-14T and the other three new isolates were between 77 and 99%. These data demonstrated that the four isolates constitute a novel species of the genus Idiomarina. Based on the phylogenetic, genotypic, phenotypic and chemotaxonomic data, the four strains represent a novel species of the genus Idiomarina, for which the name Idiomarina aquatica sp. nov. is proposed. The type strain is SN-14T ( = CCM 8471T = CECT 8360T = LMG 27613T).

scholarly journals Roseovarius nanhaiticus sp. nov., a member of the Roseobacter clade isolated from marine sediment

2010 ◽  
Vol 60 (6) ◽  
pp. 1289-1295 ◽  
Author(s):  
Baojiang Wang ◽  
Fengqin Sun ◽  
Qiliang Lai ◽  
Yaping Du ◽  
Xiupian Liu ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
Optimal Growth ◽  
Rrna Gene ◽  
Sandy Sediment ◽  
The Novel ◽  
16S Rrna Gene Sequences ◽  
Gram Staining

An aerobic, Gram-staining-negative, rod or ovoid-shaped bacterial isolate, strain NH52JT, was isolated from a sandy sediment sample from the South China Sea. Strain NH52JT exhibited tumbling motility, formed beige or faint pink colonies, gave a positive reaction in tests for catalase and oxidase and required NaCl for growth. Optimal growth was observed at pH 7.8–9.3, at 30 °C and in the presence of 2.0–4.0 % (w/v) NaCl. The novel strain did not synthesize bacteriochlorophyll a, and the DNA G+C content was 62 %. The major fatty acids were C18 : 1 ω7c, C16 : 0 and C18 : 1 ω7c 11-methyl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NH52JT was affiliated to the genus Roseovarius of the class Alphaproteobacteria. Roseovarius pacificus and Roseovarius aestuarii were the most closely related recognized species to strain NH52JT with 16S rRNA gene sequence similarity values of 95.0 and 95.7 %, respectively. Sequence similarity values between strain NH52JT and other phylogenetically related species were all below 95.0 %. Based on the phenotypic, chemotaxonomic and phylogenetic data presented, strain NH52JT is considered to represent a novel species of the genus Roseovarius, for which the name Roseovarius nanhaiticus sp. nov. is proposed. The type strain is NH52JT (=LMG 24840T=CCTCC AB 208317T=MCCC 1A03543T).

scholarly journals Rhodonellum psychrophilum gen. nov., sp. nov., a novel psychrophilic and alkaliphilic bacterium of the phylum Bacteroidetes isolated from Greenland

2006 ◽  
Vol 56 (12) ◽  
pp. 2887-2892 ◽  
Author(s):  
Mariane Schmidt ◽  
Anders Priemé ◽  
Peter Stougaard
Keyword(s):  
Gene Sequence ◽  
Unsaturated Fatty Acids ◽  
Sequence Similarity ◽  
Optimal Growth ◽  
Growth Conditions ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Psychrophilic Bacterium ◽  
The Novel ◽  
Alkaliphilic Bacterium

A novel alkaliphilic and psychrophilic bacterium was isolated from the cold and alkaline ikaite tufa columns of the Ikka Fjord in south-west Greenland. According to 16S rRNA gene sequence analysis, strain GCM71T belonged to the family ‘Flexibacteraceae’ in the phylum Bacteroidetes. Strain GCM71T, together with five related isolates from ikaite columns, formed a separate cluster with 86–93 % gene sequence similarity to their closest relative, Belliella baltica. The G+C content of the DNA from strain GCM71T was 43.1 mol%, whereas that of B. baltica was reported to be 35 mol%. DNA–DNA hybridization between strain GCM71T and B. baltica was 9.5 %. The strain was red pigmented, Gram-negative, strictly aerobic with non-motile, rod-shaped cells. The optimal growth conditions for strain GCM71T were pH 9.2–10.0, 5 °C and 0.6 % NaCl. The fatty acid profile of the novel strain was dominated by branched and unsaturated fatty acids (90–97 %), with a high abundance of iso-C17 : 1 ω9c (17.5 %), iso-C17 : 0 3-OH (17.5 %) and summed feature 3, comprising iso-C15 : 0 2-OH and/or C16 : 1 ω7c (12.6 %). Phylogenetic, chemotaxonomic and physiological characteristics showed that the novel strain could not be affiliated to any known genus. A new genus, Rhodonellum gen. nov., is proposed to accommodate the novel strain. Strain GCM71T (=DSM 17998T=LMG 23454T) is proposed as the type strain of the type species, Rhodonellum psychrophilum sp. nov.

scholarly journals Shewanella donghaensis sp. nov., a psychrophilic, piezosensitive bacterium producing high levels of polyunsaturated fatty acid, isolated from deep-sea sediments

2007 ◽  
Vol 57 (2) ◽  
pp. 208-212 ◽  
Author(s):  
Sung-Hyun Yang ◽  
Jung-Hyun Lee ◽  
Ji-Sun Ryu ◽  
Chiaki Kato ◽  
Sang-Jin Kim
Keyword(s):  
Dna Hybridization ◽  
Deep Sea ◽  
Sequence Similarity ◽  
Optimal Growth ◽  
Rrna Gene ◽  
Psychrophilic Bacterium ◽  
The Novel ◽  
16S Rrna Gene Sequences ◽  
Shewanella Frigidimarina ◽  
Deep Sea Sediments

A Gram-negative, motile, rod-shaped, psychrophilic bacterium, LT17T, was isolated from deep-sea sediments (3300 m depth) of the East Sea (Sea of Japan). Optimal growth of LT17T requires the presence of 2.5 % (w/v) NaCl, a pH of 7.0–7.5 and a temperature of 17 °C. The isolate grows optimally under a hydrostatic pressure of 10 MPa and growth is possible between 0.1 and <30 MPa. The novel strain is positive in tests for catalase, oxidase, lipase, β-glucosidase and gelatinase activities and reduces nitrate to nitrate. The predominant cellular fatty acids are iso-C13 : 0, iso-C15 : 0, C16 : 0, C16 : 1ω7 and C20 : 5ω3. The DNA G+C content of strain LT17T is 38.8 mol%. Phylogenetic analysis of 16S rRNA gene sequences places this bacterium in the class Gammaproteobacteria, within the genus Shewanella. The closest relatives of strain LT17T are Shewanella japonica (97.8 % gene sequence similarity), Shewanella pacifica (97.5 %), Shewanella olleyana (96.8 %), Shewanella frigidimarina (96.5 %) and Shewanella gelidimarina (95.4 %). The DNA–DNA hybridization levels between the novel isolate and its closest known phylogenetic relatives, S. japonica and S. pacifica, are lower than 14 %. On the basis of this polyphasic evidence, strain LT17T represents a novel species of the genus Shewanella, for which the name Shewanella donghaensis sp. nov. is proposed. The type strain is LT17T (=KCTC 10635BPT=JCM 12524T).

scholarly journals Rhodococcus Yananensis Sp. Nov., Isolated From Microbial Fermentation Bed Material From a Pig Farm

2021 ◽  
Author(s):  
Yingying Jiang ◽  
Chaochao Zheng ◽  
Tianfei Yu ◽  
Jing Li ◽  
Jiamin Ai ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Sequence Similarity ◽  
Optimal Growth ◽  
Rrna Gene ◽  
Microbial Fermentation ◽  
The Novel ◽  
Respiratory Quinone ◽  
Pig Farm ◽  
Bed Material

Abstract An opaque, pink-colored, gram-positive, aerobic bacteria (designated FBM22-1T), 0.5 to 1.0 μm in width and 0.5 to 1.5 μm in length, was isolated from microbial fermentation bed material from a pig farm in northwestern China. Optimal growth occurred at 30–37℃, pH 7.0, and 0.5% NaCl (w/v). Phylogenetic analysis based on the 16S rRNA gene sequences revealed that the novel isolate belonged to the family Nocardiaceae of the class Actinomycetia. FBM22-1T is closely related to Rhodococcus zopfii NBRC 100606T and Rhodococcus rhodochrous NBRC 16069T, with 16S rRNA gene sequence similarity of 97.95% and 97.73%, respectively. The predominant respiratory quinone in FBM22-1T was ubiquinone MK-8(H2), and the cellular fatty acids consisted primarily of C16:1ω7c/16:1ω6c, C16:0, and C18:0 10-methly1. The major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine and glycolipids. The G+C content of FBM22-1T was 68.64 mol%. Based on the phenotypic, phylogenetic, and chemotaxonomic characterization, in combination with low values of digital DNA–DNA hybridization between FBM22-1T and its closest neighbors, FBM22-1T represents a novel species of the genus Rhodococcus, for which the name Rhodococcus yananensis sp. nov. is proposed; the type strain is FBM22-1T (=KCTC 49502T = CCTCC 2020275T).

scholarly journals Salegentibacter flavus sp. nov.

2006 ◽  
Vol 56 (3) ◽  
pp. 583-586 ◽  
Author(s):  
Elena P. Ivanova ◽  
John P. Bowman ◽  
Richard Christen ◽  
Natalia V. Zhukova ◽  
Anatoly M. Lysenko ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Unsaturated Fatty Acids ◽  
Sequence Data ◽  
Sequence Similarity ◽  
Tween 80 ◽  
Optimal Growth ◽  
Rrna Gene ◽  
The Novel ◽  
Straight Chain ◽  
Branched Chain

A yellow-pigmented, non-motile, Gram-negative bacterium, designated Fg 69T, was isolated from a sediment sample collected in Chazhma Bay (Sea of Japan). The novel organism grew at 10–35 °C, was neutrophilic and required 3–10 % NaCl for optimal growth. Strain Fg 69T was able to degrade starch and to hydrolyse gelatin and Tween 80 weakly but not casein or agar. Predominant cellular fatty acids comprised n-C15 and n-C16 branched-chain and straight-chain saturated and unsaturated fatty acids, including iso-C15 : 0 (5 %), anteiso-C15 : 0 (11 %), C15 : 0 (9 %), iso-C15 : 1 (5 %), iso-C16 : 0 (8 %), C16 : 0 (5 %) and C16 : 1 ω7 (5 %) and iso- and anteiso-branched 2-OH and 3-OH C15 : 0 to C17 : 0 fatty acids (26 % in total). The G+C content of the DNA was 40·4 mol%. 16S rRNA gene sequence data indicated that strain Fg 69T belonged to the genus Salegentibacter but was distinct from recognized Salegentibacter species (94–95 % sequence similarity). Based on these results, a novel species, Salegentibacter flavus sp. nov., is proposed. The type strain is Fg 69T (=KMM 6000T=CIP 107843T).

scholarly journals Umboniibacter marinipuniceus gen. nov., sp. nov., a marine gammaproteobacterium isolated from the mollusc Umbonium costatum from the Sea of Japan

2010 ◽  
Vol 60 (3) ◽  
pp. 603-609 ◽  
Author(s):  
Lyudmila A. Romanenko ◽  
Naoto Tanaka ◽  
Galina M. Frolova
Keyword(s):  
Sea Of Japan ◽  
Sequence Similarity ◽  
Fatty Acid Analysis ◽  
Phenotypic Characterization ◽  
The Sea Of Japan ◽  
Rrna Gene ◽  
Phylogenetic Distance ◽  
The Novel ◽  
Bacterial Strains ◽  
Separate Branch

Two bacterial strains, KMM 3891T and KMM 3892, were isolated from internal tissues of the marine mollusc Umbonium costatum collected from the Sea of Japan. The novel isolates were Gram-negative, aerobic, faint pink–reddish-pigmented, rod-shaped, non-motile, stenohaline and psychrotolerant bacteria that were unable to degrade most tested complex polysaccharides. Polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Fatty acid analysis revealed C17 : 1 ω6c, C17 : 0, C16 : 0 and C16 : 1 ω7c as the dominant components. The major isoprenoid quinone was Q-7. The DNA G+C content of strain KMM 3891T was 51.7 mol%. According to phylogenetic analysis of 16S rRNA gene sequences, strains KMM 3891T and KMM 3892 were positioned within the Gammaproteobacteria as a separate branch, sharing <93 % sequence similarity to their phylogenetic relatives including Saccharophagus degradans, Microbulbifer species, Endozoicomonas elysicola, Simiduia agarivorans and Teredinibacter turnerae. Based on phenotypic characterization and phylogenetic distance, the novel marine isolates KMM 3891T and KMM 3892 represent a new genus and species, for which the name Umboniibacter marinipuniceus gen. nov., sp. nov. is proposed. The type strain of Umboniibacter marinipuniceus is KMM 3891T (=NRIC 0753T =JCM 15738T).

scholarly journals Rubritalea halochordaticola sp. nov., a carotenoid-producing verrucomicrobial species isolated from a marine chordate

2011 ◽  
Vol 61 (7) ◽  
pp. 1515-1520 ◽  
Author(s):  
Jaewoo Yoon ◽  
Satoru Matsuda ◽  
Kyoko Adachi ◽  
Hiroaki Kasai ◽  
Akira Yokota
Keyword(s):  
Dna Hybridization ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
The Novel ◽  
16S Rrna Gene Sequences ◽  
Sea Squirt ◽  
Cell Biomass ◽  
Taxonomic Approach

A Gram-negative-staining, obligately aerobic, non-motile, rod-shaped and chemoheterotrophic bacterium, designated strain MN1-1006T, was isolated from an ascidian (sea squirt) sample, and was studied using a polyphasic taxonomic approach. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the new isolate shared approximately 93–99% sequence similarity with recognized species of the genus Rubritalea within the phylum ‘Verrucomicrobia’. DNA–DNA hybridization values between strain MN1-1006T and Rubritalea squalenifaciens HOact23T and Rubritalea sabuli YM29-052T were 57% and 14.5%, respectively. Strain MN1-1006T produced carotenoid compounds that rendered the cell biomass a reddish pink colour. The strain also contained squalene. The cell-wall peptidoglycan of the novel strain contained muramic acid and meso-diaminopimelic acid. The DNA G+C content of strain MN1-1006T was 51.4 mol%. The major cellular fatty acids were iso-C14:0, iso-C16:0 and anteiso-C15:0. The major isoprenoid quinone was MK-9. On the basis of these data, it was concluded that strain MN1-1006T represents a novel species of the genus Rubritalea, for which the name Rubritalea halochordaticola sp. nov. is proposed. The type strain is MN1-1006T ( = KCTC 23186T = NBRC 107102T).

scholarly journals Thermaerobacter litoralis sp. nov., a strictly aerobic and thermophilic bacterium isolated from a coastal hydrothermal field

2006 ◽  
Vol 56 (7) ◽  
pp. 1531-1534 ◽  
Author(s):  
Reiji Tanaka ◽  
Satoshi Kawaichi ◽  
Hiroshi Nishimura ◽  
Yoshihiko Sako
Keyword(s):  
Dna Hybridization ◽  
Sequence Similarity ◽  
Thermophilic Bacterium ◽  
Hydrothermal Field ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
The Novel ◽  
Optimum Ph ◽  
Kagoshima Prefecture ◽  
Bacterium Strain

A novel thermophilic bacterium, strain KW1T, was isolated from a coastal hydrothermal field on the Satsuma Peninsula, Kagoshima Prefecture, Japan. The variably Gram-stained cells were motile rods with flagella, did not form spores and proliferated at 52–78 °C (optimum, 70 °C), pH 5–8 (optimum, pH 7) and 0–4.5 % NaCl (optimum, 1.0 %). The novel isolate was a strictly aerobic heterotroph that utilized complex proteinaceous substrates as well as a variety of carboxylic acids and amino acids. The G+C content of the genomic DNA was 70.8 mol%. Analysis of 16S rRNA gene sequences indicated that strain KW1T is closely related to Thermaerobacter subterraneus C21T (98.4 % sequence similarity). However, the DNA–DNA hybridization value for strain KW1T and T. subterraneus ATCC BAA-137T was below 46 %. On the basis of the molecular and physiological traits of strain KW1T, it represents a novel species of the genus Thermaerobacter, for which the name Thermaerobacter litoralis sp. nov. is proposed. The type strain is KW1T (=JCM 13210T=DSM 17372T).

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