An arabinose-induced enhancement of asexual reproduction and concomitant changes in metabolic state in the filamentous fungus Bipolaris maydis

Microbiology ◽  
2021 ◽  
Author(s):  
Hiroshi Yoshida ◽  
Chihiro Tanaka
Keyword(s):  
Asexual Reproduction ◽  
Vegetative Growth ◽  
Plant Cell Wall ◽  
Dry Weight ◽  
Transcriptional Analysis ◽  
Major Constituent ◽  
Phytopathogenic Fungus ◽  
Bipolaris Maydis ◽  
Carbohydrate Utilization ◽  
Physiological Basis

l-Arabinose, a major constituent pentose of plant cell-wall polysaccharides, has been suggested to be a less preferred carbon source for fungi but to be a potential signalling molecule that can cause distinct genome-wide transcriptional changes in fungal cells. Here, we explore the possibility that this unique pentose influences the morphological characteristics of the phytopathogenic fungus Bipolaris maydis strain HITO7711. When grown on plate media under different sugar conditions, the mycelial dry weight of cultures on l-arabinose was as low as that with no sugar, suggesting that l-arabinose does not substantially contribute to vegetative growth. However, the intensity of conidiation on l-arabinose was comparable to or even higher than that on d-glucose and on d-xylose, in contrast to the poor conidiation under the no-sugar condition. To explore the physiological basis of the passive growth and active conidiation on l-arabinose, we next investigated cellular responses of the fungus to these sugar conditions. Transcriptional analysis of genes related to carbohydrate metabolism showed that l-arabinose stimulates carbohydrate utilization through the hexose monophosphate shunt (HMP shunt), a catabolic pathway parallel to glycolysis and which participates in the generation of the reducing agent NADPH (the reduced form of nicotinamide adenine dinucleotide phosphate). Then, the HMP shunt was impaired by disrupting the related gene BmZwf1, which encodes glucose-6-phosphate dehydrogenase in this fungus. The resulting mutants on l-arabinose showed remarkably decreased conidiation, but a conversely increased mycelial dry weight compared with the wild-type. Our study demonstrates that l-arabinose acts to enhance resource allocation to asexual reproduction in B. maydis HITO7711 at the cost of vegetative growth, and suggests that this is mediated by the concomitant stimulation of the HMP shunt.

scholarly journals Vegetative and Reproductive Response to Fruit Load in Two Jojoba (Simmondsia chinensis) Cultivars

Agronomy ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 889
Author(s):  
Aviad Perry ◽  
Noemi Tel-Zur ◽  
Arnon Dag
Keyword(s):  
Vegetative Growth ◽  
Accumulation Rate ◽  
Dry Weight ◽  
High Yield ◽  
Lower Amount ◽  
Simmondsia Chinensis ◽  
Alternate Bearing ◽  
Fruit Removal ◽  
Growing Period ◽  
Fruit Load

Jojoba (Simmondsia chinensis) is a wax crop cultivated mainly in arid and semi-arid regions. This crop has been described as an alternate-bearing plant, meaning that it has a high-yield year (“on-year”) followed by a low-yield year (“off-year”). We investigated the effect of fruit load on jojoba’s vegetative and reproductive development. For two consecutive years, we experimented with two high-yielding cultivars—Benzioni and Hazerim—which had opposite fruit loads, i.e., one was under an on-year load, while the other was under an off-year load simultaneously. We found that removing the developing fruit from the shoot during an off-year promotes further vegetative growth in the same year, whereas in an on-year, this action has no effect. Moreover, after fruit removal in an on-year, there was a delay in vegetative growth renewal in the consecutive year, suggesting that the beginning of the growing period is dependent on the previous year’s yield load. We found that seed development in the 2018 season started a month earlier than in the 2017 season in both cultivars, regardless of fruit load. This early development was associated with higher wax content in the seeds. Hence, the wax accumulation rate, as a percentage of dry weight, was affected by year and not by fruit load. However, on-year seeds stopped growing earlier than off-year seeds, resulting in smaller seeds and an overall lower amount of wax per seed.

The influence of seed size and depth of sowing on pre-emergence and early vegetative growth of subterranean clover (Trifolium subterraneum L.)

1956 ◽  
Vol 7 (2) ◽  
pp. 98 ◽  
Author(s):  
JN Black
Keyword(s):  
Leaf Area ◽  
Seed Size ◽  
Vegetative Growth ◽  
Dry Matter ◽  
Relative Rate ◽  
Subterranean Clover ◽  
Trifolium Subterraneum ◽  
Dry Weight ◽  
Total Leaf Area ◽  
Pot Culture

Changes in the pre-emergence distribution of dry matter in subterranean clover (Trifolium subterraneum L.) variety Bacchus Marsh were followed at 21°C, using three sizes of seed and three depths of sowing, ½, 1¼, and 2 in. Decreasing seed size and increasing depth of sowing both reduce the weight of the cotyledons a t emergence. Seed of the three sizes were sown a t three depths in pot culture a t staggered intervals so that emergence was simultaneous. Dry weight in the early vegetative stage was proportional to seed size, and total leaf area and leaf numbers showed similar trends. Plants of each seed size grew at the same relative rate. No effect of depth of sowing could be detected, and this was shown to be due to the cotyledon area a t emergence being constant for any given seed size, regardless of varying depth of sowing and hence of cotyledon weight. It was concluded that seed size in a plant having epigeal germination and without endosperm is of importance: firstly, in limiting the maximum hypocotyl elongation and hence depth of sowing, and secondly, in determining cotyledon area. Cotyledon area in turn influences seedling growth, which is not affected by cotyledon weight. Once emergence has taken place, cotyledonary reserves are of no further significance in the growth of the plants.

scholarly journals Regulation of Sporangium Formation by BldD in the Rare Actinomycete Actinoplanes missouriensis

2017 ◽  
Vol 199 (12) ◽  
Author(s):  
Yoshihiro Mouri ◽  
Kenji Konishi ◽  
Azusa Fujita ◽  
Takeaki Tezuka ◽  
Yasuo Ohnishi
Keyword(s):  
Vegetative Growth ◽  
Streptomyces Coelicolor ◽  
Morphological Differentiation ◽  
Transcriptional Regulator ◽  
Saccharopolyspora Erythraea ◽  
Transcriptional Analysis ◽  
Morphological Development ◽  
Sequencing Analysis ◽  
Content Type ◽  
Biological Studies

ABSTRACT The rare actinomycete Actinoplanes missouriensis forms sporangia, including hundreds of flagellated spores that start swimming as zoospores after their release. Under conditions suitable for vegetative growth, zoospores stop swimming and germinate. A comparative proteome analysis between zoospores and germinating cells identified 15 proteins that were produced in larger amounts in germinating cells. They include an orthologue of BldD (herein named AmBldD [BldD of A. missouriensis]), which is a transcriptional regulator involved in morphological development and secondary metabolism in Streptomyces. AmBldD was detected in mycelia during vegetative growth but was barely detected in mycelia during the sporangium-forming phase, in spite of the constant transcription of AmbldD throughout growth. An AmbldD mutant started to form sporangia much earlier than the wild-type strain, and the resulting sporangia were morphologically abnormal. Recombinant AmBldD bound a palindromic sequence, the AmBldD box, located upstream from AmbldD. 3′,5′-Cyclic di-GMP significantly enhanced the in vitro DNA-binding ability of AmBldD. A chromatin immunoprecipitation-sequencing analysis and an in silico search for AmBldD boxes revealed that AmBldD bound 346 genomic loci that contained the 19-bp inverted repeat 5′-NN(G/A)TNACN(C/G)N(G/C)NGTNA(C/T)NN-3′ as the consensus AmBldD-binding sequence. The transcriptional analysis of 27 selected AmBldD target gene candidates indicated that AmBldD should repress 12 of the 27 genes, including bldM, ssgB, whiD, ddbA, and wblA orthologues. These genes are involved in morphological development in Streptomyces coelicolor A3(2). Thus, AmBldD is a global transcriptional regulator that seems to repress the transcription of tens of genes during vegetative growth, some of which are likely to be required for sporangium formation. IMPORTANCE The rare actinomycete Actinoplanes missouriensis undergoes complex morphological differentiation, including sporangium formation. However, almost no molecular biological studies have been conducted on this bacterium. BldD is a key global regulator involved in the morphological development of streptomycetes. BldD orthologues are highly conserved among sporulating actinomycetes, but no BldD orthologues, except one in Saccharopolyspora erythraea, have been studied outside the streptomycetes. Here, it was revealed that the BldD orthologue AmBldD is essential for normal developmental processes in A. missouriensis. The AmBldD regulon seems to be different from the BldD regulon in Streptomyces coelicolor A3(2), but they share four genes that are involved in morphological differentiation in S. coelicolor A3(2).

scholarly journals Pengaruh Pemberian Zeolite dan Dosis Pupuk Nitrogen Terhadap Pertumbuhan Vegetatif Jagung (Zea mays, L.) di Media Pasir Pantai

2021 ◽  
Vol 1 (1) ◽  
pp. 39-43
Author(s):  
Gunawan Budiyanto ◽  
Mulyono Mulyono ◽  
Fiyoni Dwi Setyawan
Keyword(s):  
Plant Height ◽  
Sandy Soil ◽  
Nitrogen Fertilizer ◽  
Vegetative Growth ◽  
Dry Weight ◽  
Maximum Growth ◽  
Fertilizer Application ◽  
Research Field ◽  
Maize Crop ◽  
Randomized Design

A research to study the effects of Zeolite and nitrogen fertilizer application on vegetative growth of maize crop in coastal sandy soil was conducted on the research field of Agriculture Faculty, Muhammadiyah University of Yogyakarta at Tamantirto, Bantul District of Yogyakarta during October until February, 2003. This research was aimed on the study of Zeolite application in order to enhance the nitrogen uptake in the coastal sandy soil, and its effects on vegetative growth of CP I variety of maize. The pot experiment was arranged in 7 x 3 factorial completely randomized design, with 4 replications. The first factor was Zeolite dosage consisted of 7 levels, i.e:  0, 3, 4, 5, 6, 7, and 8 %; and the second one was 3 levels nitrogen fertilizer dosage which consisted of: 75, 110, and 145 kg/ ha. The nitrogen fertilizer was applied on the medium mixed with Zeolite granules. Observations on plant height, stem diemeter, leaves number, plant fresh and dry weight were done during the vegetative growth of maize. The results showed that Zeolite application significantly enhanced the vegetative growth of CPI maize. The 5,2  - 5,6  % Zeolite was optimum to gave the maximum growth of maize crop. Nitrogen fertilizer application was also significantly increased the plant height and biomass weight, and the 145 kg/ha was the optimum dosage. There was no interaction between Zeolite and nitrogen fertilizer application in order to affected the maize growth.

scholarly journals EFFECT OF DIPPING IN GIBBERLLIC ACID AND SPRAYING NUTRIENT SOLUTION AGRO LEAF ON SOME VEGETATIVE GROWTH AND YIELD OF JERUSALUM ARTICHOKE.

2016 ◽  
Vol 47 (4) ◽  
Author(s):  
Sadik & et al.
Keyword(s):  
Leaf Area ◽  
Nutrient Solution ◽  
Vegetative Growth ◽  
Dry Weight ◽  
Factorial Experiment ◽  
Growth And Yield ◽  
Control Treatment ◽  
Highest Weight ◽  
Number Of Leaves ◽  
Number Of Branches

This study was conducted in experimental fields, Department of Horticulture, University of Bagdad, in Abu-Graib  during season 2011-2012 for jerusalum artichoke. This study was included the effect of dipping tubers in three concentrations of GA3(2.5,5,10g/l) (G1,G2,G3),as well as to control treatment (G0), and spraying nutrient solution Agro leaf A1 (8g/l), as well as to control treatment (A0). This study was made by using Factorial experiment (4*2) within the design RCBD with three replicates. Results could be summarized as follows: G3A0 increased field emergence (12.00 day), G2A0 increased percentage of germination (99.33%) and G2A1 increased number of branches (4.60 stem.plant-1) but the treatment G1A1gave highest rate for number of leaves, leaf area, guide of leaf area, dry weight of the vegetative parts and dry weight of 100gm tubers as(4495.10 leaf.plant-1, 2246.20 dsm2, 99.84, 922.40g, 24.00g.) respectively. The treatments gave significant differences quantity yield, so treatment G3A0 gave highest weight of the tubers as(45.55g.) but the treatment G3A1 gave highest number of the tubers as(68.00 tuber.plant-1 ) and highest yield of plant as (2890g/plant).

scholarly journals Draft Genome Sequence and Transcriptional Analysis of Rosellinia necatrix Infected with a Virulent Mycovirus

2018 ◽  
Vol 108 (10) ◽  
pp. 1206-1211 ◽  
Author(s):  
Takeo Shimizu ◽  
Satoko Kanematsu ◽  
Hajime Yaegashi
Keyword(s):  
Genome Sequence ◽  
Molecular Mechanisms ◽  
Plant Cell Wall ◽  
Draft Genome ◽  
Transcriptional Analysis ◽  
Draft Genome Sequence ◽  
Effective Control ◽  
Economic Losses ◽  
Rosellinia Necatrix ◽  
Cell Wall Degrading Enzymes

Understanding the molecular mechanisms of pathogenesis is useful in developing effective control methods for fungal diseases. The white root rot fungus Rosellinia necatrix is a soilborne pathogen that causes serious economic losses in various crops, including fruit trees, worldwide. Here, using next-generation sequencing techniques, we first produced a 44-Mb draft genome sequence of R. necatrix strain W97, an isolate from Japan, in which 12,444 protein-coding genes were predicted. To survey differentially expressed genes (DEGs) associated with the pathogenesis of the fungus, the hypovirulent W97 strain infected with Rosellinia necatrix megabirnavirus 1 (RnMBV1) was used for a comprehensive transcriptome analysis. In total, 545 and 615 genes are up- and down-regulated, respectively, in R. necatrix infected with RnMBV1. Gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses of the DEGs suggested that primary and secondary metabolism would be greatly disturbed in R. necatrix infected with RnMBV1. The genes encoding transcriptional regulators, plant cell wall-degrading enzymes, and toxin production, such as cytochalasin E, were also found in the DEGs. The genetic resources provided in this study will accelerate the discovery of genes associated with pathogenesis and other biological characteristics of R. necatrix, thus contributing to disease control.

Dry Matter, Nodulation and Nutrient Uptake in Chickpea (Cicer arietinum) as Influenced by Irrigation and Phosphorus

1989 ◽  
Vol 25 (3) ◽  
pp. 349-355 ◽  
Author(s):  
S. S. Parihar ◽  
R. S. Tripathi
Keyword(s):  
Grain Yield ◽  
Nutrient Uptake ◽  
Irrigation Water ◽  
Vegetative Growth ◽  
Dry Matter ◽  
Dry Weight ◽  
Irrigation Scheduling ◽  
Dry Matter Accumulation ◽  
Pan Evaporation ◽  
Eastern India

SUMMARYThe response of chickpea to irrigation and phosphorus was studied at Kharagpur in Eastern India. Irrigation scheduling was based on the ratio between irrigation water applied and cumulative pan evaporation (ID/CPE), and had little effect on dry matter accumulation. Increasing the frequency and amount of irrigation reduced the number and dry weight of nodules per plant, which increased to a maximum 70 days after sowing and then declined. Irrigation significantly reduced grain yield as a result of excessive vegetative growth at the expense of pod formation. Application of phosphorus promoted nodulation and increased both nodule dry weight and the concentration of N, P and K in grain and stover. Uptake of N, P and K by the crop was also increased.

Application of Potassium Fertilizer and Chicken Coop Fertilizer Against Growth and Production of Shallots (Allium ascalanicum L.)

Nabatia ◽  
2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Andy Pradana ◽  
Al Machfudz WDP
Keyword(s):  
Growth Phase ◽  
Vegetative Growth ◽  
Growth Parameters ◽  
Block Design ◽  
Dry Weight ◽  
Chicken Manure ◽  
Fertilizer Treatment ◽  
Potassium Fertilizer ◽  
Wet Weight ◽  
Number Of Leaves

This study aims to determine the application of potassium fertilizer and chicken manure to the growth and production of shallots (Allium ascalanicum L.). The study used a randomized block design with 2 factors repeated 3 times. Factor 1: Potassium Fertilizer 40,60,80 kg/ha. Factor 2: Chicken Manure 5,10,15 tons/ha. The results showed that the application of potassium fertilizer and chicken manure did not interact with either the growth phase or the production phase of the plant on all observation variables, while the potassium fertilizer treatment showed significantly different results only on the plant vegetative growth parameters, namely the number of leaves at the age of 7 days after planting, namely ( 8,111 strands), while the vegetative phase had no significant effect on the number of tubers per clump, wet weight of tubers per clump, wet weight of tubers per plot, dry weight of sun-dried tubers per clump, and dry weight of sun-dried tubers per plot. While the treatment of chicken manure had a significant effect on the vegetative growth phase of plants, namely the length of plants at 28 DAP (30.278 cm) and 35 DAP (32.704 cm), on the number of leaves at 7 DAP (8,000 strands). While the generative phase (60 DAP) significantly affected the number of tubers per clump, namely (5,889 grams), wet weight of tubers per clump (38,333 grams), wet weight of tubers per plot (319,000 grams), dry weight of sun-dried tubers per clump. namely (23.074 grams), and the dry weight of sun-dried tubers per plot (191.889 grams).

scholarly journals Metabolism and Development during Conidial Germination in Response to a Carbon-Nitrogen-Rich Synthetic or a Natural Source of Nutrition inNeurospora crassa

mBio ◽  
2019 ◽  
Vol 10 (2) ◽  
Author(s):  
Zheng Wang ◽  
Cristina Miguel-Rojas ◽  
Francesc Lopez-Giraldez ◽  
Oded Yarden ◽  
Frances Trail ◽  
...  
Keyword(s):  
Life History ◽  
Sexual Reproduction ◽  
Nitrogen Metabolism ◽  
Asexual Reproduction ◽  
Sexual Development ◽  
Vegetative Growth ◽  
Synthetic Medium ◽  
Conidial Germination ◽  
Content Type ◽  
Genes Expression

ABSTRACTFungal spores germinate and undergo vegetative growth, leading to either asexual or sexual reproductive dispersal. Previous research has indicated that among developmental regulatory genes, expression is conserved across nutritional environments, whereas pathways for carbon and nitrogen metabolism appear highly responsive—perhaps to accommodate differential nutritive processing. To comprehensively investigate conidial germination and the adaptive life history decision-making underlying these two modes of reproduction, we profiled transcription ofNeurospora crassagerminating on two media: synthetic Bird medium, designed to promote asexual reproduction; and a natural maple sap medium, on which both asexual reproduction and sexual reproduction manifest. A later start to germination but faster development was observed on synthetic medium. Metabolic genes exhibited altered expression in response to nutrients—at least 34% of the genes in the genome were significantly downregulated during the first two stages of conidial germination on synthetic medium. Knockouts of genes exhibiting differential expression across development altered germination and growth rates, as well as in one case causing abnormal germination. A consensus Bayesian network of these genes indicated especially tight integration of environmental sensing, asexual and sexual development, and nitrogen metabolism on a natural medium, suggesting that in natural environments, a more dynamic and tentative balance of asexual and sexual development may be typical ofN. crassacolonies.IMPORTANCEOne of the most remarkable successes of life is its ability to flourish in response to temporally and spatially varying environments. Fungi occupy diverse ecosystems, and their sensitivity to these environmental changes often drives major fungal life history decisions, including the major switch from vegetative growth to asexual or sexual reproduction. Spore germination comprises the first and simplest stage of vegetative growth. We examined the dependence of this early life history on the nutritional environment using genome-wide transcriptomics. We demonstrated that for developmental regulatory genes, expression was generally conserved across nutritional environments, whereas metabolic gene expression was highly labile. The level of activation of developmental genes did depend on current nutrient conditions, as did the modularity of metabolic and developmental response network interactions. This knowledge is critical to the development of future technologies that could manipulate fungal growth for medical, agricultural, or industrial purposes.

Controlled-temperature effects on cotton growth and development

1998 ◽  
Vol 130 (4) ◽  
pp. 451-462 ◽  
Author(s):  
D. ROUSSOPOULOS ◽  
A. LIAKATAS ◽  
W. J. WHITTINGTON
Keyword(s):  
Leaf Area ◽  
Temperature Variation ◽  
Vegetative Growth ◽  
Growth And Development ◽  
Dry Weight ◽  
Maximum Temperature ◽  
Node Number ◽  
Mean Temperature ◽  
Fruiting Branch ◽  
Threshold Temperatures

The growth and development of cotton was studied on cotton plants grown in pots in growth rooms under constant day/night temperature (C) and varying temperature regimes throughout the day and/or night (V) The V-treatments had a common mean temperature of 22°C. The objectives were to determine the thermal requirements of three cultivars and to observe the extent of genotype×thermal environment interactions throughout the entire growth period.Vegetative growth was found to be almost exclusively time and temperature dependent, varietal differences being largely insignificant. Plant material was found to accumulate four times faster under the warmest C-regime, which was 7°C warmer than the coolest. The C-treatments caused variation in the number and size of lateral shoots and leaves, causing leaf area to be larger at the squaring stage in cool environments but at maturity in warm regimes. Relatively cool nights lowered the position of the first floral or fruiting branch, whereas warm days shortened flowering intervals and thus promoted earliness. However, the effect of temperature in altering the position of the first floral branch seems to be less important than its effect on the shedding of early squares.The effects of fluctuating temperature (V) on vegetative growth and earliness were similar to those from constant temperature environments. Growth and development rates were low in the low minimum V-regimes, especially when the maximum temperature was also low. Temperature variation affected vegetative growth to a greater extent in the early than in the later stages of development. At squaring, leaf area and dry weight were lowest under the regime with the highest minimum and maximum temperatures. Later on, only leaf area at flowering and total dry weight at maturity significantly differed between treatments. High maximum or minimum temperatures produced effects similar to a higher or lower mean temperature, respectively. The low minimum raised the node number of the first floral or fruiting branch, whereas in the case of boll dry weight it acted in the same way as a further drop in temperature, decreasing the weight, regardless of the maximum temperature. Boll period was affected mainly by the temperature variation itself rather by than the type of variation.The inverse of time to a certain stage and the corresponding mean temperature were linearly related and allowed threshold temperatures (T0) and thermal time requirements to be estimated. T0=12°C, except for the initial stage, when it was lower. A cotton growing season cooler on average by only 1°C will considerably delay maturity.

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