scholarly journals LcpRVH2 – regulating the expression of latex-clearing proteins in Gordonia polyisoprenivorans VH2

Microbiology ◽  
2019 ◽  
Vol 165 (3) ◽  
pp. 343-354 ◽  
Author(s):  
Sylvia Oetermann ◽  
Rense Jongsma ◽  
Anna Coenen ◽  
Jeanne Keller ◽  
Alexander Steinbüchel
Keyword(s):  
Gordonia Polyisoprenivorans

scholarly journals Cloning and Characterization of α-Methylacyl Coenzyme A Racemase from Gordonia polyisoprenivorans VH2

2008 ◽  
Vol 74 (22) ◽  
pp. 7085-7089 ◽  
Author(s):  
Quyen Arenskötter ◽  
Jens Heller ◽  
David Dietz ◽  
Matthias Arenskötter ◽  
Alexander Steinbüchel
Keyword(s):  
Cytochrome P450 ◽  
Reverse Transcription ◽  
Kanamycin Resistance ◽  
P450 Monooxygenase ◽  
Transcription Analysis ◽  
Kanamycin Resistance Cassette ◽  
Short Chain Dehydrogenase ◽  
Gordonia Polyisoprenivorans ◽  
Rubber Degradation

ABSTRACT The mcr gene of Gordonia polyisoprenivorans VH2 is not clustered with genes required for rubber degradation. Its disruption by insertion of a kanamycin resistance cassette impaired growth on methyl-branched isoprenoids but not on linear hydrocarbons. Intact mcr from this bacterium or from Nocardia farcinica IFM 10152 complemented the mutant. Reverse transcription analysis showed similar mcr VH2 expression results during cultivation with poly(cis-1,4-isoprene) and propionate. Additional genes coding for a putative cytochrome P450 monooxygenase and a short-chain dehydrogenase/reductase involved in β-oxidation and poly(cis-1,4-isoprene) degradation were also characterized.

scholarly journals Global Regulator of Rubber Degradation in Gordonia polyisoprenivorans VH2: Identification and Involvement in the Regulation Network

2020 ◽  
Vol 86 (15) ◽  
Author(s):  
Jan de Witt ◽  
Sylvia Oetermann ◽  
Mariana Parise ◽  
Doglas Parise ◽  
Jan Baumbach ◽  
...  
Keyword(s):  
Intergenic Region ◽  
Degradation Pathway ◽  
Receptor Protein ◽  
Waste Materials ◽  
Regulation Mechanism ◽  
Global Regulator ◽  
Content Type ◽  
Rubber Waste ◽  
Gordonia Polyisoprenivorans ◽  
Rubber Degradation

ABSTRACT A cAMP receptor protein (CRPVH2) was detected as a global regulator in Gordonia polyisoprenivorans VH2 and was proposed to participate in the network regulating poly(cis-1,4-isoprene) degradation as a novel key regulator. CRPVH2 shares a sequence identity of 79% with GlxR, a well-studied global regulator of Corynebacterium glutamicum. Furthermore, CRPVH2 and GlxR have a common oligomerization state and similar binding motifs, and thus most likely have similar functions as global regulators. Size exclusion chromatography of purified CRPVH2 confirmed the existence as a homodimer with a native molecular weight of 44.1 kDa in the presence of cAMP. CRPVH2 bound to the TGTGAN6TCACT motif within the 131-bp intergenic region of divergently oriented lcp1VH2 and lcpRVH2, encoding a latex clearing protein and its putative repressor, respectively. DNase I footprinting assays revealed the exact operator size of CRPVH2 in the intergenic region (25 bp), which partly overlapped with the proposed promoters of lcpRVH2 and lcp1VH2. Our findings indicate that CRPVH2 represses the expression of lcpRVH2 while simultaneously directly or indirectly activating the expression of lcp1VH2 by binding the competing promoter regions. Furthermore, binding of CRPVH2 to upstream regions of additional putative enzymes of poly(cis-1,4-isoprene) degradation was verified in vitro. In silico analyses predicted 206 CRPVH2 binding sites comprising 244 genes associated with several functional categories, including carbon and peptide metabolism, stress response, etc. The gene expression regulation of several subordinated regulators substantiated the function of CRPVH2 as a global regulator. Moreover, we anticipate that the novel lcpR regulation mechanism by CRPs is widespread in other rubber-degrading actinomycetes. IMPORTANCE In order to develop efficient microbial recycling strategies for rubber waste materials, it is required that we understand the degradation pathway of the polymer and how it is regulated. However, only little is known about the transcriptional regulation of the rubber degradation pathway, which seems to be upregulated in the presence of the polymer. We identified a novel key regulator of rubber degradation (CRPVH2) that regulates several parts of the pathway in the potent rubber-degrader G. polyisoprenivorans VH2. Furthermore, we provide evidence for a widespread involvement of CRP regulators in the degradation of rubber in various other rubber-degrading actinomycetes. Thus, these novel insights into the regulation of rubber degradation are essential for developing efficient microbial degradation strategies for rubber waste materials by this group of actinomycetes.

scholarly journals Enzymatic activity of catechol 1,2-dioxygenase and catechol 2,3-dioxygenase produced by Gordonia polyisoprenivorans

Química Nova ◽  
2012 ◽  
Vol 35 (8) ◽  
pp. 1587-1592 ◽  
Author(s):  
Andréa Scaramal Silva ◽  
Flávio Anastácio de Oliveira Camargo ◽  
Robson Andreazza ◽  
Rodrigo Josemar Seminoti Jacques ◽  
Daiana Bortoluzzi Baldoni ◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Catechol 1,2 Dioxygenase ◽  
Gordonia Polyisoprenivorans

scholarly journals Identification and Application of Plasmids Suitable for Transfer of Foreign DNA to Members of the Genus Gordonia

2003 ◽  
Vol 69 (8) ◽  
pp. 4971-4974 ◽  
Author(s):  
Matthias Arenskötter ◽  
Dirk Baumeister ◽  
Rainer Kalscheuer ◽  
Alexander Steinbüchel
Keyword(s):  
Heat Shock ◽  
Gene Transfer ◽  
Plasmid Dna ◽  
Dna Transfer ◽  
Autonomous Replication ◽  
Recombinant Strains ◽  
Foreign Dna ◽  
Gordonia Polyisoprenivorans

ABSTRACT Gene transfer systems for Gordonia polyisoprenivorans strains VH2 and Y2K based on electroporation and conjugation, respectively, were established. Several parameters were optimized, resulting in transformation efficiencies of >4 × 105 CFU/μg of plasmid DNA. In contrast to most previously described electroporation protocols, the highest efficiencies were obtained by applying a heat shock after the intrinsic electroporation. Under these conditions, transfer and autonomous replication of plasmid pNC9503 was also demonstrated to proceed in G. alkanivorans DSM44187, G. nitida DSM44499T, G. rubropertincta DSM43197T, G. rubropertincta DSM46038, and G. terrae DSM43249T. Conjugational plasmid DNA transfer to G. polyisoprenivorans resulted in transfer frequencies of up to 5 × 10−6 of the recipient cells. Recombinant strains capable of polyhydroxyalkanoate synthesis from alkanes were constructed.

Survival and biodegradation activity of Gordonia polyisoprenivorans 135: Basics of a biosensor receptor

2017 ◽  
Vol 53 (5) ◽  
pp. 580-586 ◽  
Author(s):  
E. V. Emelyanova ◽  
N. E. Souzina ◽  
V. N. Polivtseva ◽  
A. N. Reshetilov ◽  
I. P. Solyanikova
Keyword(s):  
Gordonia Polyisoprenivorans

Gordonia polyisoprenivorans Septicemia in a Bone Marrow Transplant Patient

2004 ◽  
Vol 23 (3) ◽  
pp. 226-228 ◽  
Author(s):  
V. A. J. Kempf ◽  
M. Schmalzing ◽  
A. F. Yassin ◽  
K. P. Schaal ◽  
D. Baumeister ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Transplant ◽  
Transplant Patient ◽  
Marrow Transplant ◽  
Bone Marrow Transplant Patient ◽  
Gordonia Polyisoprenivorans
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