scholarly journals Pseudomonas aeruginosa variants obtained from veterinary clinical samples reveal a role for cyclic di-GMP in biofilm formation and colony morphology

Microbiology ◽  
2017 ◽  
Vol 163 (11) ◽  
pp. 1613-1625 ◽  
Author(s):  
Maria T. Brock ◽  
Galya C. Fedderly ◽  
Grace I. Borlee ◽  
Michael M. Russell ◽  
Liliana K. Filipowska ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Biofilm Formation ◽  
Colony Morphology ◽  
Clinical Samples

scholarly journals Evaluation of Antibiotic Resistance Pattern, Alginate and Biofilm Production in Clinical Isolates of Pseudomonas aeruginosa

2021 ◽  
Author(s):  
Fateme DAVARZANI ◽  
Navid SAIDI ◽  
Saeed BESHARATI ◽  
Horieh SADERI ◽  
Iraj RASOOLI ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Biofilm Formation ◽  
Bacterial Resistance ◽  
Antimicrobial Agents ◽  
Clinical Isolates ◽  
Diffusion Method ◽  
Clinical Samples ◽  
Resistance Pattern ◽  
Alginate Production ◽  
Opportunistic Bacteria

Background: Pseudomonas aeruginosa is one of the most common opportunistic bacteria causing nosocomial infections, which has significant resistance to antimicrobial agents. This bacterium is a biofilm and alginate producer. Biofilm increases the bacterial resistance to antibiotics and the immune system. Therefore, the present study was conducted to investigate the biofilm formation, alginate production and antimicrobial resistance patterns in the clinical isolates of P. aeruginosa. Methods: One hundred isolates of P. aeruginosa were collected during the study period (from Dec 2017 to Jul 2018) from different clinical samples of the patients admitted to Milad and Pars Hospitals at Tehran, Iran. Isolates were identified and confirmed by phenotypic and genotypic methods. Antimicrobial susceptibility was specified by the disk diffusion method. Biofilm formation and alginate production were measured by microtiter plate and carbazole assay, respectively. Results: Sixteen isolates were resistant to all the 12 studied antibiotics. Moreover, 31 isolates were MultidrugResistant (MDR). The highest resistance rate was related to ofloxacin (36 isolates) and the least resistance was related to piperacillin-tazobactam (21 isolates). All the isolates could produce the biofilm and alginate. The number of isolates producing strong, medium and weak biofilms was equal to 34, 52, and 14, respectively. Alginate production was more than 400 μg/ml in 39 isolates, 250-400 μg/ml in 51 isolates and less than 250 μg/ml in 10 isolates. Conclusion: High prevalence of MDR, biofilm formation, and alginate production were observed among the clinical isolates of P. aeruginosa. The results also showed a significant relationship between the amount of alginate production and the level of biofilm formation.

Assessment of pelA-carried Pseudomonas aeruginosa isolates in respect to biofilm formation

2019 ◽  
pp. 1180-1187
Author(s):  
Mahmood Abd AL- Razzaq Hassan AL-Sheikhly ◽  
Laith N. Musleh ◽  
Harith J. F. Al-Mathkhury
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Nosocomial Infections ◽  
Biofilm Formation ◽  
Clinical Samples ◽  
Antibacterial Resistance ◽  
Gentamicin Resistance ◽  
Pcr Method

Owing to high antibacterial resistance of Pseudomonas aeruginosa, it could be considered as the main reason behind the nosocomial infections. P. aeruginosa has a well-known biofilm forming ability. The expression of polysaccharide encoding locus (pelA gene) by P. aeruginosa is essential for this ability. The purpose of the current research was to determine the biofilm formation in P. aeruginosa isolated from clinical samples and to evaluate the role of the selected PelA gene in biofilm formation using PCR method in Iraqi patients. Results revealed that 24 (96%) isolates were found to have the ability to form biofilm that was remarkably related to gentamicin resistance. Moreover, the pelA gene was found in all biofilm-producers. In conclusion, the results of the current study revealed that the P. aeruginosa biofilm-producer isolates were resistant to the antibiotics in question. Likewise, because of wide spreading, it appears that the pelA gene is related to biofilm formation.

scholarly journals Role of biofilm-specific antibiotic resistance genes PA0756-0757, PA5033 and PA2070 in Pseudomonas aeruginosa

2018 ◽  
Author(s):  
Prasanth Manohar ◽  
Thamaraiselvan Shanthini ◽  
Reethu Ann Philip ◽  
Subramani Ramkumar ◽  
Manali Kale ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Biofilm Formation ◽  
Tamil Nadu ◽  
Antibiotic Resistance Genes ◽  
Clinical Samples ◽  
Cross Sectional ◽  
Antibiotic Resistant ◽  
Resistant Genes

AbstractTo evaluate the presence of biofilm-specific antibiotic-resistant genes, PA0756-0757, PA5033 and PA2070 in Pseudomonas aeruginosa isolated from clinical samples in Tamil Nadu. For this cross-sectional study, 24 clinical isolates (included pus, urine, wound, and blood) were collected from two diagnostic centers in Chennai from May 2015 to February 2016. Biofilm formation was assessed using microtiter dish biofilm formation assay and minimal inhibitory concentration (MIC) and minimal bactericidal concentrations (MBC) were determined for planktonic and biofilm cells (MBC assay). Further, PCR amplification of biofilm-specific antibiotic resistance genes PA0756-0757, PA5033 and PA2070 were performed. Biofilm formation was found to be moderate/strong in 16 strains. MBC for planktonic cells showed that 4, 7, 10 and 14 strains were susceptible to gentamicin, ciprofloxacin, meropenem and colistin respectively. In MBC assay for biofilm cells (MBC-B), all the 16 biofilm producing strains were resistant to ciprofloxacin and gentamicin whereas nine and four were resistant to meropenem, and colistin respectively. The biofilm-specific antibiotic-resistant genes PA0756-0757 was found in 10 strains, 6 strains with PA5033 and 9 strains with PA2070 that were found to be resistant phenotypically. This study highlighted the importance of biofilm-specific antibiotic resistance genes PA0756-0757, PA5033, and PA2070 in biofilm-forming P. aeruginosa.

scholarly journals Biofilm inhibitor taurolithocholic acid alters colony morphology, specialized metabolism, and virulence ofPseudomonas aeruginosa

2019 ◽  
Author(s):  
Alanna R. Condren ◽  
Lisa Juliane Kahl ◽  
George Kritikos ◽  
Manuel Banzhaf ◽  
Lars E. P. Dietrich ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacterial Community ◽  
Biofilm Formation ◽  
Colony Morphology ◽  
Biofilm Inhibition ◽  
Specialized Metabolism ◽  
Virulence Assay ◽  
Biofilm Inhibitor ◽  
Highly Virulent ◽  
Wax Moth

AbstractBiofilm inhibition by exogenous molecules has been an attractive strategy for the development of novel therapeutics. We investigated the biofilm inhibitor taurolithocholic acid (TLCA) and its effects on the specialized metabolism, virulence and biofilm formation of the clinically relevant bacteriumPseudomonas aeruginosastrain PA14. Our study shows that TLCA alters specialized metabolism, thereby affectingP. aeruginosacolony biofilm physiology. We observed an upregulation of metabolites correlated to virulence such as the siderophore pyochelin. A wax moth virulence assay confirmed that treatment with TLCA increases virulence ofP. aeruginosa. Based on our results, we believe that future endeavors to identify biofilm inhibitors must consider how a putative lead is altering the specialized metabolism of a bacterial community to prevent pathogens from entering a highly virulent state.

Phenotypic Detection and Biofilm Formation among Pseudomonas aeruginosa Isolated from Different Sites of Infection

2019 ◽  
Vol 10 (02) ◽  
Author(s):  
Ilham A Bunyan ◽  
Oday M Hadi ◽  
Hussein A K Al-Mansoori
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Virulence Factors ◽  
Biofilm Formation ◽  
Trichloroacetic Acid ◽  
Egg Yolk ◽  
Staining Technique ◽  
Protease Production ◽  
Clinical Samples ◽  
Bacterial Biofilms ◽  
Vitek 2

In The present study, included the collection of (100) samples from different clinical sites. Clinical samples were collected from patients who were visit and admitted All-Hilla teaching hospital at the period from November (2017) to February (2018). Cultural, biochemical and VITEK2 system were used for identification, and depending on the VITEK2 system (VITEK-2 GN Kit), revealed that twenty one (21) Pseudomonas aeruginosa isolates were recovered, The percentage conformational identification of P. aeruginosa was performed using VITEK2 system of (21) P. aeruginosa was (99%). Nine(42.8%) samples were isolated from burns, 5(23.8%) samples from wound, 3(14.2%) from urine, 2(9.5%) from ear swab, and 1(4.7%) sample was isolated from both blood and sputum. The phenotypic detection of some virulence factors for all isolates were detected. Detection of capsule was done by using capsule staining technique was carried out for P. aeruginosa isolates; it was found that all P. aeruginosa isolates (100%) have a capsule surrounding the bacterial cell. Hemolysin production by P. aeruginosa was studied; it was found that 12(57.1%) isolates were able to produce extracellular hemolysin on blood agar. P. aeruginosa isolates were also investigated for their ability to produce siderophores. The results showed that 9(42.8%) isolates of P. aeruginosa were able to produce siderophores. Protease production by P. aeruginosa isolates was studied; it was found that all these isolates (100%) have this enzyme as appear as a zone around the colony when being grown on (M9) media after adding of (3ml) of (5%) Trichloroacetic acid and incubation for (24 hrs.). Ability of P. aeruginosa to produce lipase has been investigated; it found that all these isolates (100%) were able to produce lipase after incubation for (48 hrs.) on egg yolk agar. Also, bacterial biofilms cause chronic diseases that are difficult to control and in the present study, differentiation of bacteria as biofilm producers and non-biofilm producers was done by using (ELISA) TCP method, a total of (21) isolates were tested for their ability to produce biofilm. From these isolates, (19) isolates were form strong biofilm, (2) isolates were form moderate biofilm.

Association between biofilm formation gene bla exoU and metallo and extend spectrum beta-lactamase production of multidrug resistance Pseudomonas aeruginosa in clinical samples

2021 ◽  
Vol 24 ◽  
Author(s):  
Fattma Abodi Ali
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Biofilm Formation ◽  
Public Hospitals ◽  
High Rate ◽  
Clinical Samples ◽  
Antimicrobial Drugs ◽  
Product Size ◽  
Vitek 2 ◽  
Extend Spectrum Beta Lactamase ◽  
Control Management

Background: The presence of biofilm formation exoU gene is significant challenge to infection control management in hospitals and exposure by Pseudomonas aeruginosa may lead to further spread and development of antimicrobial resistance. Methods: Out of 227 samples 40 clinical isolates of P. aeruginosa were collected from patients attending public hospitals ( Rizgary, Teaching hospital, Laboratory center, Raparin, Nanakaly hospitals)in Erbil city/Iraq over a period during 2018 to march 2019 and fully characterized by standard bacteriological procedures and antimicrobial susceptibility test and ESBL has been carried out by Vitek 2 compact system and. by Vitek 2 compact system. The identification has been verified by all isolates as P. aeruginosa by using 16S rDNA with product size (956pb). Results: A high rate of resistance was seen against Penicillin and lincomycin and Piperacillin and chloramphenicol and rifampicin (100 %), whereas Imipenem (5%) were found to be the most effective antimicrobial drugs. Of all P. aeruginosa isolates, 30 (75% %) were identified as MDR, approximately 9(22.5%) of isolates were resistant to 9 drugs in burn samples. Quantitative biofilm determination using the Congo red method revealed that 28 isolates (70%) produced biofilm, biofilm production was significantly higher among MDR P. aeruginosa isolates while coproduction of Extended Spectrum β-lactamase (ESBL) together with Metallo β-lactamase (MBL) ESBLs MBLs recorded in (52.5%) of the isolates. Altogether 40 isolates were processed for analysis by PCR assays and showed that 26(70%) of P. aeruginosa isolates harboured the exoU encoding gene with product size (204) pb was more commonly seen in isolates obtained from burn isolates. In addition, exo U gene was significantly associated with the higher MDR (80%), 8 isolates (76.9%)had exoU gene with ESBL and( 65%) had MBL and the same for MDR (80.8%) in samples for burning. Conclusion: Our results showed surveillance of P. aeruginosa resistance against antimicrobial and ESBL and MBL is fundamental to monitor trends in susceptibility patterns and appropriately guide clinicians in choosing empirical or directed therapy.

Biofilm formation and serum susceptibility in Pseudomonas aeruginosa

Open Medicine ◽  
2014 ◽  
Vol 9 (2) ◽  
pp. 187-192 ◽  
Author(s):  
Greta Mikucionyte ◽  
Asta Dambrauskiene ◽  
Erika Skrodeniene ◽  
Astra Vitkauskiene
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Respiratory Tract ◽  
Biofilm Formation ◽  
Clinical Samples ◽  
Opportunistic Pathogens ◽  
Carbapenem Resistant ◽  
Biofilm Production ◽  
Significant Difference ◽  
Resistant Strains ◽  
Different Sources

AbstractPseudomonas aeruginosa (P. aeruginosa) is one of the most important opportunistic pathogens. The pathogenicity of P. aeruginosa has been associated with multiple bacterial virulence factors. The aim of this study was to evaluate the association between P. aeruginosa strains obtained from various clinical samples and resistance to antibiotics and pathogenicity factors, such as resistance to serum bactericidal activity and biofilm formation. This study included 121 P. aeruginosa strains isolated from clinical samples; 65 of the isolated P. aeruginosa strains were carbapenem-resistant, and 56 were carbapenem-sensitive. Carbapenem-resistant P. aeruginosa strains were more often resistant to the majority of tested antibiotics, compared to carbapenem-sensitive strains. We did not find any statistically significant difference between resistance to carbapenems and serum resistance and ability of tested P. aeruginosa strains to produce biofilms. Carbapenem-resistant P. aeruginosa strains were recovered from the urinary tract significantly more often (75.0%) than carbapenem-sensitive P. aeruginosa strains (25.0%). Carbapenem-sensitive P. aeruginosa strains were recovered significantly more often from the respiratory tract than carbapenem-resistant strains, 60.0% and 40.0%, respectively. All the P. aeruginosa strains recovered from blood were serum-resistant. P. aeruginosa strains recovered from the respiratory tract and wounds were significantly frequently serum sensitive, 95.6% and 56.6%, respectively. We did not find any differences in biofilm production among the P. aeruginosa strains recovered from different sources.

scholarly journals Evaluation of blaGES-5 and bla veb-1 genes with multidrug-resistant extend, pandrug resistance patterns (MDR, XDR, PDR), and biofilm formation in Pseudomonas aeruginosa isolates

2021 ◽  
Vol 67 (3) ◽  
pp. 52-60
Author(s):  
Fattma A. Ali ◽  
Sevan Hassan Bakir ◽  
Sayran Hamed Haji ◽  
Bashdar M. Hussen
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Biofilm Formation ◽  
Phylogenetic Trees ◽  
Antibiotic Sensitivity ◽  
Multidrug Resistant ◽  
Clinical Samples ◽  
Sensitivity Testing ◽  
Automated Method ◽  
Resistance Patterns ◽  
Vitek 2

Pseudomonas aeruginosa is a ubiquitous microorganism that is difficult to treat due to the increasing prevalence of multidrug resistance patterns. A total of 227 samples were taken from different clinical samples during the study period from January 2018 to December 2018. The isolates were identified with antibiotic sensitivity testing with ESBL by the Vitek-2 automated method. MDR, XDR, and PDR were determined. 40 (17.6%) isolates were positive for P. aeruginosa, maximum of ESBL and MBL. Positive isolates were detected in the burn, coexisting ESBL + MBL enzymes in 21 (52.5%) of our isolates. Imipenem followed by Meropenem were found to be effective against ESBL and MBL producers. Resistance was reached between 72-100% to 5 antibiotics. The frequency of PDR, MDR, and XDR were 5%, 50%, and 45%, respectively. The frequency of co-production between MDR, XDR, and PDR with MBL, ESBL, and Biofilm was 35%, 12.5% and 5%, respectively. Among the ESBLs, the frequency of distribution of bla VEB-1gene and blaGES-5 gene was 50% and 40 %, respectively. Bacterial isolates simultaneously carrying blaVEB-1 gene with multiple ?-lactamases of different classes of biofilm, MDR, PDR, and XDR as same as a coexisting blaGES-5 gene. One isolate was detected as new isolates registered in global gene bank as locally P. aeruginosa isolates in Erbil city (LOCUS MN900953). The phylogenetic trees of the blaVEB gene isolates were demonstrated a genotype closely related to others, deposited in GenBank similar to the P. aeruginosa gene; gene sequencing revealed a 99% similarity with other isolates deposited in GenBank.

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