scholarly journals Amphotericin B induces apoptosis-like programmed cell death in Naegleria fowleri and Naegleria gruberi

Microbiology ◽  
2017 ◽  
Vol 163 (7) ◽  
pp. 940-949 ◽  
Author(s):  
Roberto Cárdenas-Zúñiga ◽  
Angélica Silva-Olivares ◽  
José D' Artagnan Villalba-Magdaleno ◽  
Virginia Sánchez-Monroy ◽  
Jesús Serrano-Luna ◽  
...  
Keyword(s):  
Cell Death ◽  
Programmed Cell Death ◽  
Amphotericin B ◽  
Naegleria Fowleri ◽  
Nægleria Fowleri ◽  
Naegleria Gruberi

Exploring the Anti-Infective Value of Inuloxin A Isolated from Inula viscosa against the Brain-Eating Amoeba (Naegleria fowleri) by Activation of Programmed Cell Death

2020 ◽  
Vol 12 (1) ◽  
pp. 195-202
Author(s):  
Ikrame Zeouk ◽  
Ines Sifaoui ◽  
Aitor Rizo-Liendo ◽  
Iñigo Arberas-Jiménez ◽  
María Reyes-Batlle ◽  
...  
Keyword(s):  
Cell Death ◽  
Programmed Cell Death ◽  
Naegleria Fowleri ◽  
Nægleria Fowleri ◽  
The Brain

The type 2 statins, cerivastatin, rosuvastatin and pitavastatin eliminate Naegleria fowleri at low concentrations and by induction of programmed cell death (PCD)

2021 ◽  
pp. 104784
Author(s):  
Aitor Rizo-Liendo ◽  
Iñigo Arberas-Jiménez ◽  
Inés Sifaoui ◽  
María Reyes-Batlle ◽  
José E. Piñero ◽  
...  
Keyword(s):  
Cell Death ◽  
Programmed Cell Death ◽  
Naegleria Fowleri ◽  
Low Concentrations ◽  
Nægleria Fowleri

scholarly journals Naphthyridine Derivatives Induce Programmed Cell Death in Naegleria fowleri

2021 ◽  
Vol 14 (10) ◽  
pp. 1013
Author(s):  
Aitor Rizo-Liendo ◽  
Iñigo Arberas-Jiménez ◽  
Endika Martin-Encinas ◽  
Ines Sifaoui ◽  
María Reyes-Batlle ◽  
...  
Keyword(s):  
Cell Death ◽  
Programmed Cell Death ◽  
Opportunistic Pathogen ◽  
Cellular Membrane ◽  
Water Bodies ◽  
Ros Generation ◽  
Naegleria Fowleri ◽  
Trophozoite Stage ◽  
Nægleria Fowleri

Primary amoebic encephalitis (PAM) caused by the opportunistic pathogen Naegleria fowleri is characterized as a rapid and lethal infection of the brain which ends in the death of the patient in more than 90% of the reported cases. This amoeba thrives in warm water bodies and causes infection after individuals perform risky activities such as splashing or diving, mostly in non-treated water bodies such as lakes and ponds. Moreover, the infection progresses very fast and no fully effective molecules have currently been found to treat PAM. In this study, naphthyridines fused with chromenes or chromenones previously synthetized by the group were tested in vitro against the trophozoite stage of two strains of N. fowleri. In addition, the most active molecule was evaluated in order to check the induction of programmed cell death (PCD) in the treated amoebae. Compound 3 showed good anti-Naegleria activity (61.45 ± 5.27 and 76.61 ± 10.84 µM, respectively) against the two different strains (ATCC® 30808 and ATCC® 30215) and a good selectivity compared to the cytotoxicity values (>300 µM). In addition, it was able to induce PCD, causing DNA condensation, damage at the cellular membrane, reduction in mitochondrial membrane potential and ATP levels, and ROS generation. Hence, naphthyridines fused with chromenes or chromenones could be potential therapeutic agents against PAM in the near future.

scholarly journals Fluvastatin and atorvastatin induce programmed cell death in the brain eating amoeba Naegleria fowleri

2020 ◽  
Vol 130 ◽  
pp. 110583
Author(s):  
Aitor Rizo-Liendo ◽  
Ines Sifaoui ◽  
Iñigo Arberas-Jiménez ◽  
María Reyes-Batlle ◽  
José E. Piñero ◽  
...  
Keyword(s):  
Cell Death ◽  
Programmed Cell Death ◽  
Naegleria Fowleri ◽  
Nægleria Fowleri ◽  
The Brain

scholarly journals Synergistic Activities of Azithromycin and Amphotericin B against Naegleria fowleri In Vitro and in a Mouse Model of Primary Amebic Meningoencephalitis

2006 ◽  
Vol 51 (1) ◽  
pp. 23-27 ◽  
Author(s):  
Shannon M. Soltow ◽  
George M. Brenner
Keyword(s):  
Central Nervous System ◽  
Mouse Model ◽  
Amphotericin B ◽  
Fixed Combination ◽  
Central Nervous System Infection ◽  
Naegleria Fowleri ◽  
Combined Use ◽  
Nægleria Fowleri ◽  
Primary Amebic Meningoencephalitis

ABSTRACT Naegleria fowleri is responsible for producing a rapidly fatal central nervous system infection known as primary amebic meningoencephalitis (PAM). To date, amphotericin B, an antifungal agent, is the only agent with established clinical efficacy in the treatment of PAM. However, amphotericin B is not always successful in treating PAM and is associated with severe adverse effects. We previously found azithromycin to be more effective than amphotericin B in a mouse model of PAM. We therefore investigated the combination of amphotericin B and azithromycin in vitro and in a mouse model of PAM. For the in vitro studies, 50% inhibitory concentrations were calculated for each drug alone and for the drugs in fixed combination ratios of 1:1, 3:1, and 1:3. We found amphotericin B and azithromycin to be synergistic at all three of the fixed combination ratios. In our mouse model of PAM, a combination of amphotericin B (2.5 mg/kg of body weight) and azithromycin (25 mg/kg) protected 100% of the mice, whereas amphotericin B alone (2.5 mg/kg) protected only 27% of mice and azithromycin alone (25 mg/kg) protected 40% of mice. This study indicates that amphotericin B and azithromycin are synergistic against the Lee strain of N. fowleri, suggesting that the combined use of these agents may be beneficial in treating PAM.

Naegleria fowleri and Naegleria gruberi 20S proteasome: identification and characterization

2020 ◽  
Vol 99 (5) ◽  
pp. 151085
Author(s):  
Paula Guzmán-Téllez ◽  
Diana Martínez-Valencia ◽  
Angélica Silva-Olivares ◽  
Rosa M. del Ángel ◽  
Jesús Serrano-Luna ◽  
...  
Keyword(s):  
20S Proteasome ◽  
Naegleria Fowleri ◽  
Nægleria Fowleri ◽  
Identification And Characterization ◽  
Naegleria Gruberi

Cytopathogenicity of Naegleria fowleri and Naegleria gruberi for Established Mammalian Cell Cultures

1982 ◽  
Vol 68 (6) ◽  
pp. 1110 ◽  
Author(s):  
F. M. Marciano-Cabral ◽  
M. Patterson ◽  
D. T. John ◽  
S. Q. Bradley
Keyword(s):  
Mammalian Cell ◽  
Cell Cultures ◽  
Naegleria Fowleri ◽  
Mammalian Cell Cultures ◽  
Nægleria Fowleri ◽  
Naegleria Gruberi

Oleic Acid Coated Silver Nanoparticles Showed Better in Vitro Amoebicidal Effects against Naegleria fowleri than Amphotericin B

2019 ◽  
Vol 11 (16) ◽  
pp. 2431-2437 ◽  
Author(s):  
Kavitha Rajendran ◽  
Ayaz Anwar ◽  
Naveed Ahmed Khan ◽  
Zara Aslam ◽  
Muhammad Raza Shah ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Oleic Acid ◽  
Amphotericin B ◽  
Naegleria Fowleri ◽  
Nægleria Fowleri
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