scholarly journals Specific Bacillus subtilis 168 variants form biofilms on nutrient-rich medium

Microbiology ◽  
2016 ◽  
Vol 162 (11) ◽  
pp. 1922-1932 ◽  
Author(s):  
Ramses Gallegos-Monterrosa ◽  
Eisha Mhatre ◽  
Ákos T. Kovács
Keyword(s):  
Bacillus Subtilis ◽  
Rich Medium ◽  
Bacillus Subtilis 168 ◽  
Nutrient Rich Medium

scholarly journals SleC Is Essential for Germination of Clostridium difficile Spores in Nutrient-Rich Medium Supplemented with the Bile Salt Taurocholate

2009 ◽  
Vol 192 (3) ◽  
pp. 657-664 ◽  
Author(s):  
David A. Burns ◽  
John T. Heap ◽  
Nigel P. Minton
Keyword(s):  
Health Care ◽  
Bacillus Subtilis ◽  
Clostridium Difficile ◽  
Disease Transmission ◽  
Health Care Services ◽  
Lytic Enzyme ◽  
Rich Medium ◽  
Infectious Diarrhea ◽  
Care Services ◽  
Nutrient Rich Medium

ABSTRACT Clostridium difficile is the major cause of infectious diarrhea and a major burden to health care services. The ability of this organism to form endospores plays a pivotal role in infection and disease transmission. Spores are highly resistant to many forms of disinfection and thus are able to persist on hospital surfaces and disseminate infection. In order to cause disease, the spores must germinate and the organism must grow vegetatively. Spore germination in Bacillus is well understood, and genes important for this process have recently been identified in Clostridium perfringens; however, little is known about C. difficile. Apparent homologues of the spore cortex lytic enzyme genes cwlJ and sleB (Bacillus subtilis) and sleC (C. perfringens) are present in the C. difficile genome, and we describe inactivation of these homologues in C. difficile 630Δerm and a B1/NAP1/027 clinical isolate. Spores of a sleC mutant were unable to form colonies when germination was induced with taurocholate, although decoated sleC spores formed the same number of heat-resistant colonies as the parental control, even in the absence of germinants. This suggests that sleC is absolutely required for conversion of spores to vegetative cells, in contrast to CD3563 (a cwlJ/sleB homologue), inactivation of which had no effect on germination and outgrowth of C. difficile spores under the same conditions. The B1/NAP1/027 strain R20291 was found to sporulate more slowly and produce fewer spores than 630Δerm. Furthermore, fewer R20291 spores germinated, indicating that there are differences in both sporulation and germination between these epidemic and nonepidemic C. difficile isolates.

Crystal Structure of Urate Oxidase from Bacillus Subtilis 168

2019 ◽  
Vol 64 (7) ◽  
pp. 1126-1133 ◽  
Author(s):  
A. Nayab ◽  
S. A. Moududee ◽  
Y. Shi ◽  
Y. Jiang ◽  
Q. Gong
Keyword(s):  
Crystal Structure ◽  
Bacillus Subtilis ◽  
Urate Oxidase ◽  
Bacillus Subtilis 168

In vitro characterization of N-terminal truncated EpsC from Bacillus subtilis 168, a UDP-N-acetylglucosamine 4,6-dehydratase

2018 ◽  
Vol 657 ◽  
pp. 78-88 ◽  
Author(s):  
Chinmayi R. Kaundinya ◽  
Handanahal S. Savithri ◽  
K. Krishnamurthy Rao ◽  
Petety V. Balaji
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Subtilis 168 ◽  
In Vitro Characterization
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