scholarly journals Role of dihydrofolate reductase in tetrahydrobiopterin biosynthesis and lipid metabolism in the oleaginous fungus Mortierella alpina

Microbiology ◽  
2016 ◽  
Vol 162 (9) ◽  
pp. 1544-1553 ◽  
Author(s):  
Hongchao Wang ◽  
Chen Zhang ◽  
Jinghan Feng ◽  
Yuan Liu ◽  
Qin Yang ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Dihydrofolate Reductase ◽  
Mortierella Alpina ◽  
Oleaginous Fungus

scholarly journals Role of Malic Enzyme during Fatty Acid Synthesis in the Oleaginous Fungus Mortierella alpina

2014 ◽  
Vol 80 (9) ◽  
pp. 2672-2678 ◽  
Author(s):  
G. Hao ◽  
H. Chen ◽  
L. Wang ◽  
Z. Gu ◽  
Y. Song ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Synthesis ◽  
Malic Enzyme ◽  
Acid Synthesis ◽  
Mortierella Alpina ◽  
Oleaginous Fungus

The role of phenylalanine hydroxylase in lipogenesis in the oleaginous fungus Mortierella alpina

Microbiology ◽  
2021 ◽  
Vol 167 (8) ◽  
Author(s):  
Hongchao Wang ◽  
Chunmei Wang ◽  
Weiwei Yuan ◽  
Haiqin Chen ◽  
Wenwei Lu ◽  
...  
Keyword(s):  
Fatty Acid Biosynthesis ◽  
Phenylalanine Hydroxylase ◽  
Transcript Level ◽  
Cellular Fatty Acid ◽  
Industrial Applications ◽  
Mortierella Alpina ◽  
Limiting Factor ◽  
Wide Range ◽  
Oleaginous Fungus

Phenylalanine hydroxylase (PAH) catalyses the irreversible hydroxylation of phenylalanine to tyrosine, which is the rate-limiting reaction in phenylalanine metabolism in animals. A variety of polyunsaturated fatty acids can be synthesized by the lipid-producing fungus Mortierella alpina, which has a wide range of industrial applications in the production of arachidonic acid. In this study, RNA interference (RNAi) with the gene PAH was used to explore the role of phenylalanine hydroxylation in lipid biosynthesis in M. alpina. Our results indicated that PAH knockdown decreased the PAH transcript level by approximately 55% and attenuated cellular fatty acid biosynthesis. Furthermore, the level of NADPH, which is a critical reducing agent and the limiting factor in lipogenesis, was decreased in response to PAH RNAi, in addition to the downregulated transcription of other genes involved in NADPH production. Our study indicates that PAH is part of an overall enzymatic and regulatory mechanism supplying NADPH required for lipogenesis in M. alpina.

Lipid metabolism research in oleaginous fungus Mortierella alpina: Current progress and future prospects

2021 ◽  
pp. 107794
Author(s):  
Lulu Chang ◽  
Hengqian Lu ◽  
Haiqin Chen ◽  
Xin Tang ◽  
Jianxin Zhao ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Mortierella Alpina ◽  
Future Prospects ◽  
Oleaginous Fungus

scholarly journals The role of MTHFDL in mediating intracellular lipogenesis in oleaginous Mortierella alpina

Microbiology ◽  
2020 ◽  
Vol 166 (7) ◽  
pp. 617-623
Author(s):  
Hongchao Wang ◽  
Qizai Wang ◽  
Chen Zhang ◽  
Haiqin Chen ◽  
Wenwei Lu ◽  
...  
Keyword(s):  
Regulatory Mechanism ◽  
Acid Synthesis ◽  
Mortierella Alpina ◽  
Limiting Factor ◽  
Transcript Levels ◽  
Methylenetetrahydrofolate Dehydrogenase ◽  
Oleaginous Fungus ◽  
Methylene Tetrahydrofolate ◽  
Folate Cycle

The oleaginous fungus Mortierella alpina can synthesize a variety of polyunsaturated fatty acids, which are used extensively in industry for the production of arachidonic acid (AA). NADPH is the limiting factor and critical reducing agent in lipid biosynthesis. In the folate cycle, methylenetetrahydrofolate dehydrogenase (MTHFDL) catalyzes the conversion of methylene tetrahydrofolate into 10-formyl-tetrahydrofolate with the reduction of NADP+ to NADPH. MTHFDL RNAi was used to investigate the role of the folate cycle in lipogenesis. Gene knockdown decreased the transcript levels of MTHFDL by about 50 % and attenuated cell fatty acid synthesis. The observation of decreased NADPH levels and downregulated NADPH-producing genes in response to MTHFDL RNAi indicates a novel aspect of the NADPH regulatory mechanism. Thus, our study demonstrates that MTHFDL plays key role in the mediation of NADPH in lipogenesis in M. alpina.

Glucose disposal and lipid metabolism in man: role of thyroid hormones

1988 ◽  
Vol 117 (4_Suppl) ◽  
pp. S130-S131
Author(s):  
M. J. MÜLLER ◽  
A. G. BURGER ◽  
E. JEQUIER ◽  
K.J. ACHESON
Keyword(s):  
Lipid Metabolism ◽  
Thyroid Hormones ◽  
Glucose Disposal

Genetic background of cholesterol absorption efficacy. The role of Niemann–Pick C1-like 1 receptor and its genetic variation in lipid metabolism

2010 ◽  
Vol 151 (34) ◽  
pp. 1376-1383 ◽  
Author(s):  
Mariann Harangi ◽  
István Balogh ◽  
János Harangi ◽  
György Paragh
Keyword(s):  
Genetic Variation ◽  
Lipid Metabolism ◽  
Genetic Background ◽  
Cholesterol Absorption ◽  
Niemann Pick

A Niemann–Pick C1-like-1 egy szterolfelismerő domént tartalmazó membránfehérje, amelyet nagy számban expresszálnak csúcsi felszínükön a bélhámsejtek. Az utóbbi évek vizsgálatai azt igazolták, hogy ez a fehérje szükséges a szabad koleszterin bejutásához a bélhámsejtekbe a bél lumenéből. Biokémiai vizsgálatok azt igazolták, hogy a Niemann–Pick C1-like-1-hez kötődik az ezetimib, amely egy hatékony koleszterinfelszívódást gátló szer. A bélből történő koleszterinfelszívódás ütemében és az ezetimibkezelés hatékonyságában tapasztalt egyéni eltérések hátterében felmerült néhány Niemann–Pick C1-like-1 génvariáció oki szerepe.

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