scholarly journals Purification and characterization of immunogenic recombinant virus-like particles of porcine circovirus type 2 expressed in silkworm pupae

2018 ◽  
Vol 99 (7) ◽  
pp. 917-926 ◽  
Author(s):  
Akitsu Masuda ◽  
Jae Man Lee ◽  
Takeshi Miyata ◽  
Tetsuo Sato ◽  
Shizuka Hayashi ◽  
...  
2011 ◽  
Vol 8 (1) ◽  
pp. 88 ◽  
Author(s):  
Tippawan Jantafong ◽  
Alongkot Boonsoongnern ◽  
Pariwat Poolperm ◽  
Kitcha Urairong ◽  
Chalermpol Lekcharoensuk ◽  
...  

2000 ◽  
Vol 13 (2) ◽  
pp. 143-153 ◽  
Author(s):  
ROMAN M. POGRANICHNYY ◽  
KYOUNG-JIN YOON ◽  
PERRY A. HARMS ◽  
SABRINA L. SWENSON ◽  
JEFFREY J. ZIMMERMAN ◽  
...  

2017 ◽  
Vol 49 (5) ◽  
pp. 1071-1075 ◽  
Author(s):  
José Paulo Hiroji Sato ◽  
Danielle Gava ◽  
Rejane Schaefer ◽  
Maurício Egídio Cantão ◽  
Janice Reis Ciacci-Zanella ◽  
...  

2014 ◽  
Vol 169 (3-4) ◽  
pp. 147-153 ◽  
Author(s):  
Dong-Jun An ◽  
Seong-In Lim ◽  
Yong Kwan Kim ◽  
Hyun-Kyoung Lee ◽  
Yoon-Young Cho ◽  
...  

2020 ◽  
Vol 16 (1) ◽  
Author(s):  
Yu Tao ◽  
Rui Yang ◽  
Jianhong Shu ◽  
Wenqian Zheng ◽  
Jian Chen ◽  
...  

Abstract Background Mycoplasma hyopneumoniae (Mhp) and porcine circovirus type 2 (PCV2) are two important pathogens causing Mycoplasma pneumonia of swine (MPS) and porcine circovirus diseases and porcine circovirus-associated diseases (PCVDs/PCVADs), respectively, and resulted in considerable economic loss to the swine industry worldwide. Currently, vaccination is one of the main measures to control these two diseases; however, there are few combination vaccines that can prevent these two diseases. To determine the effect of combination immunization, we developed capsid-derived (Cap) virus-like particles (VLPs) of PCV2 and a new recombinant chimera composed of the P97R1, P46, and P42 antigens of Mhp. Then we investigated the immune responses induced by the immunization with this combination vaccine in mice and piglets. Results The high level antibodies against three protein antigens (P97R1, P46, and P42 of Mhp) were produced after immunization, up to or higher than 1:400,000; the antibody levels in Pro group continuously increased throughout the 42 days for all the antigens tested. The lymphocyte proliferative response in PCV2 group was stronger than that in PBS, VP, Mhp CV in mice. The antibody levels for Cap remained stable and reached the peak at 35 DAI. The IFN-γ and IL-4 in sera were significantly enhanced in the Pro group than that in the negative control-VP group on Day 14 and 28 post-the first immunization in piglets. Conclusions Above all, the combination immunization could induce humoral and cellular immune responses against all four antigens in mice and piglets. Therefore, our approach is a simple and effective vaccination strategy to protect pigs against MPS and PCVD/PCVAD.


2014 ◽  
Vol 11 (1) ◽  
Author(s):  
Maria Antonia Rincón Monroy ◽  
Gloria Consuelo Ramirez-Nieto ◽  
Victor Julio Vera ◽  
Jairo Jaime Correa ◽  
Jose Dario Mogollón-Galvis

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