scholarly journals Naganishia floricola sp. nov., a novel basidiomycetous yeast species isolated from flowers of Sorbaria sorbifolia

2020 ◽  
Vol 70 (8) ◽  
pp. 4496-4501 ◽  
Author(s):  
Yu Zhou ◽  
Bi-Si Jia ◽  
Yu-Guang Zhou ◽  
Ai-Hua Li ◽  
Lu Xue
Keyword(s):  
Phylogenetic Analysis ◽  
Rna Polymerase Ii ◽  
Novel Species ◽  
Large Subunit ◽  
Elongation Factor ◽  
Sequence Divergence ◽  
Its Region ◽  
Small Subunit ◽  
Basidiomycetous Yeast ◽  
D2 Domain

Two yeast strains representing a novel species in the basidiomycetous yeast genus Naganishia were isolated from flowers of Sorbaria sorbifolia collected in Beijing Olympic Forest Park, PR China. Results of multi-gene phylogenetic analysis indicated that the two strains were closely related to the type strains of Naganishia bhutanensis (CBS 6294T) and Naganishia antarctica (CBS 7687T). However, the new isolates differed from N. bhutanensis CBS 6294T by 1.79 % sequence divergence in the D1/D2 domain (11 nt substitutions and three indels), and 2.42 % (15 nt differences and one indel) to N. antarctica CBS 7687T. In the ITS region, the new isolates showed 1.15 % divergence (7 nt substitutions and one indel) to N. bhutanensis CBS 6294T and 0.92 % divergence (5 nt substitutions and no indels) to N. antarctica CBS 7687T. A phylogenetic analysis employing the sequences of six genes (D1/D2 domain of large subunit rDNA, ITS, small subunit rDNA, two subunits of the RNA polymerase II and elongation factor-1α) indicated that the novel species belonged to the genus Naganishia and formed a well-supported clade with N. bhutanensis, N. antarctica and N. indica. Moreover, the two strains differed from their closest relatives by the ability to grow on distinct carbon and nitrogen sources and ability to grow at 30 °C. On the basis of these findings, we propose a novel species in the genus Naganishia (Filobasidiales), Naganishia floricola sp. nov. (holotype CGMCC 2.5856).

scholarly journals Savitreea pentosicarens gen. nov., sp. nov., a yeast species in the family Saccharomycetaceae isolated from a grease trap

2020 ◽  
Vol 70 (11) ◽  
pp. 5665-5670
Author(s):  
Varunya Sakpuntoon ◽  
Jirameth Angchuan ◽  
Chanita Boonmak ◽  
Pannida Khunnamwong ◽  
Noémie Jacques ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Yeast Species ◽  
Sequence Divergence ◽  
Its Region ◽  
Rrna Gene ◽  
Nucleotide Substitutions ◽  
D2 Domain ◽  
The Family ◽  
Lsu Rrna Gene ◽  
Lsu Rrna

Two strains (DMKU-GTCP10-8 and CLIB 1740) representing a novel anamorphic yeast species were isolated from a grease sample collected from a grease trap in Thailand and from an unidentified fungus collected in French Guiana, respectively. On the basis of phylogenetic analysis based on the combined D1/D2 domain of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region, Lachancea fermentati CBS 707T was the closely related species with 12.8 % sequence divergence (70 nucleotide substitutions and three gaps in 571 nucleotides) and 28.1 % sequence divergence (93 nucleotide substitutions and 90 gaps in 651 nucleotides) in the D1/D2 domain of the LSU rRNA gene and the ITS region, respectively. Phylogenetic analysis based on the concatenated sequences of the five genes including the small subunit rRNA gene, the D1/D2 domain of the LSU rRNA gene, the ITS region, translation elongation factor-1 alpha (TEF1) and RNA polymerase II subunit 2 (RPB2) genes confirmed that the two strains (DMKU-GTCP10-8 and CLIB 1740) were well-separated from other described yeast genera in Saccharomycetaceae. Hence, Savitreea pentosicarens gen. nov., sp. nov. is proposed to accommodate these two strains as members of the family Saccharomycetaceae. The holotype is S. pentosicarens DMKU-GTCP10-8T (ex-type strain TBRC 12159=PYCC 8490; MycoBank number 835044).

scholarly journals Five Fungal Pathogens Are Responsible for Bayberry Twig Blight and Fungicides Were Screened for Disease Control

2020 ◽  
Vol 8 (5) ◽  
pp. 689 ◽  
Author(s):  
Wenjun Li ◽  
Ming Hu ◽  
Yang Xue ◽  
Zhijun Li ◽  
Yanfei Zhang ◽  
...  
Keyword(s):  
Ribosomal Rna ◽  
Fungal Pathogens ◽  
Large Subunit ◽  
Elongation Factor ◽  
Morphological Characteristics ◽  
Its Region ◽  
Small Subunit ◽  
Field Application ◽  
Financial Loss ◽  
Twig Blight

Bayberry (Myrica rubra) is a commercial fruit in China. For the past seven years, twig blight disease has been attacking bayberry plantations in Shantou City, Guangdong Province, China, leading to destructive damage and financial loss. In this study, five fungal species associated with twig dieback and stem blight were identified based on morphological characteristics combined with multilocus sequence analysis (MLSA) on the internal transcribed spacer (ITS) region, partial sequences of β-tubulin (tub2), translation elongation factor 1-α (tef1-α), large subunit ribosomal RNA (LSU) and small subunit ribosomal RNA (SSU) genes, which are Epicoccum sorghinum, Neofusicoccum parvum, Lasiodiplodia theobromae, Nigrospora oryzae and a Pestalotiopsis new species P. myricae. P. myricae is the chief pathogen in fields, based on its high isolation rate and fast disease progression after inoculation. To our knowledge, this is the first study reporting the above five fungi as the pathogens responsible for bayberry twig blight. Indoor screening of fungicides indicates that Prochloraz (copper salt) is the most promising fungicide for field application, followed by Pyraclostrobin, 15% Difenoconazole + 15% Propiconazole, Difenoconazole and Myclobutanil. Additionally, Bacillus velezensis strain 3–10 and zeamines from Dickeya zeae strain EC1 could be used as potential ecofriendly alternatives to control the disease.

Sympodiomycopsis yantaiensis sp. nov., a basidiomycetous yeast isolated from insect frass

2013 ◽  
Vol 63 (Pt_9) ◽  
pp. 3501-3505 ◽  
Author(s):  
Liang Chen ◽  
Lin Zhang ◽  
Zhi-Hui Li ◽  
Feng-Li Hui
Keyword(s):  
Type Strain ◽  
Large Subunit ◽  
Its Region ◽  
Basidiomycetous Yeast ◽  
Rrna Gene ◽  
The Novel ◽  
Sophora Japonica ◽  
Closely Related Species ◽  
D2 Domain ◽  
Lsu Rrna Gene

Two strains (NYNU 121010T and NYNU 121032) of a novel basidiomycetous yeast species belonging to the genus Sympodiomycopsis were isolated from insect frass collected from trunks of a pagoda tree (Sophora japonica L.) in Yantai, Shandong province, east China. The sequence analyses of the D1/D2 domain of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region indicated that the closest relatives were Sympodiomycopsis kandeliae FIRDI 007T, Sympodiomycopsis paphiopedili CBS 7429T and Sympodiomycopsis sp. S6A. The D1/D2 sequences of the novel strains differed by 12 nt substitutions (2 %) from the type strain of S. kandeliae, and by 13 nt substitutions (2.2 %) from the type strain of S. paphiopedili and from Sympodiomycopsis sp. S6A. The novel strains differed from closely related species by more than 4.6 % substitutions in the ITS region. The novel strains can also be distinguished from S. kandeliae and S. paphiopedili on the basis of a number of morphological and physiological characteristics and represent a novel species in the genus Sympodiomycopsis, for which the name Sympodiomycopsis yantaiensis sp. nov. is proposed. The type strain is NYNU 121010T ( = CICC 32998T = CBS 12813T). The Mycobank deposit number is MB 804119.

scholarly journals Candida andamanensis sp. nov., Candida laemsonensis sp. nov. and Candida ranongensis sp. nov., anamorphic yeast species isolated from estuarine waters in a Thai mangrove forest

2011 ◽  
Vol 61 (2) ◽  
pp. 454-461 ◽  
Author(s):  
Somjit Am-In ◽  
Savitree Limtong ◽  
Wichien Yongmanitchai ◽  
Sasitorn Jindamorakot
Keyword(s):  
Phylogenetic Analysis ◽  
Sequence Analysis ◽  
Type Strain ◽  
Mangrove Forest ◽  
Yeast Species ◽  
Novel Species ◽  
Large Subunit ◽  
Rrna Gene ◽  
D2 Domain ◽  
Large Subunit Rrna

Five strains (RV5T, RV140, R31T, RS17 and RS28T) representing three novel anamorphic ascomycetous yeast species were isolated by membrane filtration from estuarine waters collected from a mangrove forest in Laem Son National Park, Ranong Province, Thailand, on different occasions. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, sequence analysis of the D1/D2 domain of the large-subunit rRNA gene and the internal transcribed spacer region and phylogenetic analysis, three strains were found to represent two novel Candida species. Two strains (RV5T and RV140) represented a single novel species, for which the name Candida laemsonensis sp. nov. is proposed. The type strain is RV5T (=BCC 35154T =NBRC 105873T =CBS 11419T). Strain R31T was assigned to a novel species that was named Candida andamanensis sp. nov. (type strain R31T =BCC 25965T =NBRC 103862T =CBS 10859T). On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, sequence analysis of the D1/D2 domain of the large-subunit rRNA gene and phylogenetic analysis, strains RS17 and RS28T represented another novel species of Candida, for which the name Candida ranongensis sp. nov. is proposed. The type strain is RS28T (=BCC 25964T =NBRC 103861T =CBS 10861T).

scholarly journals Four new species in the Tremella fibulifera complex (Tremellales, Basidiomycota)

MycoKeys ◽  
2021 ◽  
Vol 82 ◽  
pp. 33-56
Author(s):  
Long-Fei Fan ◽  
Renato Lúcio Mendes Alvarenga ◽  
Tatiana Baptista Gibertoni ◽  
Fang Wu ◽  
Yu-Cheng Dai
Keyword(s):  
New Species ◽  
Rna Polymerase Ii ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Elongation Factor ◽  
Morphological Characteristics ◽  
Molecular Data ◽  
Small Subunit ◽  
Molecular Evidence ◽  
Rrna Gene

Samples of species close to Tremella fibulifera from China and Brazil are studied, and T. fibulifera is confirmed as a species complex including nine species. Five known species (T. cheejenii, T. fibulifera s.s., T. “neofibulifera”, T. lloydiae-candidae and T. olens) and four new species (T. australe, T. guangxiensis, T. latispora and T. subfibulifera) in the complex are recognized based on morphological characteristics, molecular evidence, and geographic distribution. Sequences of eight species of the complex were included in the phylogenetic analyses because T. olens lacks molecular data. The phylogenetic analyses were performed by a combined sequence dataset of the internal transcribed spacer (ITS) and the partial nuclear large subunit rDNA (nLSU), and a combined sequence dataset of the ITS, partial nLSU, the small subunit mitochondrial rRNA gene (mtSSU), the translation elongation factor 1-α (TEF1), the largest and second largest subunits of RNA polymerase II (RPB1 and RPB2). The eight species formed eight independent lineages with robust support in phylogenies based on both datasets. Illustrated description of the six species including Tremella fibulifera s.s., T. “neofibulifera” and four new species, and discussions with their related species, are provided. A table of the comparison of the important characteristics of nine species in the T. fibulifera complex and a key to the whitish species in Tremella s.s. are provided.

scholarly journals Genetic Analysis and Pathogenic Characterization of Alternaria tenuissima Induced Fruit Rot of Bitter Gourd

2021 ◽  
Vol 22 (2) ◽  
Author(s):  
SEHRISH IFTIKHAR ◽  
WAHEED ANWAR ◽  
Adnan Akhter ◽  
SAJID ALI ◽  
HAFIZ AZHAR ALI KHAN ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Rna Polymerase Ii ◽  
Large Subunit ◽  
Elongation Factor ◽  
Its Region ◽  
First Record ◽  
Fruit Rot ◽  
Bitter Gourd ◽  
Alternaria Tenuissima

Abstract. Iftikhar S, Anwar W, Akhter A, Ali S, Khan HAA, Khurshid M, Haider MS. 2021. Genetic analysis and pathogenic characterization of Alternaria tenuissima induced fruit rot of bitter gourd. Biodiversitas 22: 617-625. Bitter gourd (Momordica charantia Linn.), belongs to Cucurbitaceae family, is widely cultivated in areas with warm climate. In 2017, fruits of bitter gourd-bearing rot symptoms were observed in the Punjab province of Pakistan. The disease-causing fungal isolate was collected from the diseased fruits on potato dextrose agar (PDA). Microscopic examination revealed short conidiophores arose singly, measuring 79.8- 158.5 μm long and 3.94-7.89 μm thick. The size of conidia varied from 25.7 to 46.45 μm and 8.55-14.39 μm in length and width respectively, which were characteristics of Alternaria spp. To confirm the identity and molecular characterization of the isolate, the internal transcribed spacer (ITS) region, translation elongation factor 1 alpha (TEF1-α), glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and RNA polymerase II large subunit 2 (RPB2) genes were amplified. The sequence analysis of amplicons and phylogenetic studies specified the homology of isolated Alternaria spp. with the previously reported A. tenuissima in GeneBank. The pathogenicity tests conducted on the fruits of bitter gourd confirmed the disease development with typical Alternaria induced rot symptoms, thus satisfied Koch's postulate. To our knowledge, this is the first record of A. tenuissima causing fruit rot of bitter gourd in Pakistan.

New species of Echinoderma and Lepiota (Agaricaceae) from China

Phytotaxa ◽  
2020 ◽  
Vol 447 (4) ◽  
pp. 221-236
Author(s):  
YA-JUN HOU ◽  
ZAI-WEI GE
Keyword(s):  
Rna Polymerase Ii ◽  
Large Subunit ◽  
Elongation Factor ◽  
Its Region ◽  
Morphological Characters ◽  
Molecular Evidence ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
The Tropics ◽  
Elongation Factor 1

Three species of Lepiota sensu lato from China are described and illustrated based on morphological characters and molecular evidence. Echinoderma flavidoasperum and Lepiota omninoflava are new to science, while Lepiota echinacea is newly reported from China. Echinoderma flavidoasperum is characterized by a yellow pileus, nearly smooth stipe that discolors light red when bruised, and subcylindrical basidiospores. Lepiota omninoflava, so far only found in the tropics, is characterized by the yellow basidiomata, ellipsoid basidiospores and the absence of cheilocystidia. All three of the Chinese species are discussed and placed within a phylogeny based on the internal transcribed spacer (ITS) region, the large subunit (LSU) of the ribosomal DNA, the translation elongation factor 1-α (tef1-α) and the second largest subunit of RNA polymerase II (rpb2).

Morphology and phylogeny of Pseudorobillarda eucalypti sp. nov., from Thailand

Phytotaxa ◽  
2014 ◽  
Vol 176 (1) ◽  
pp. 251 ◽  
Author(s):  
NARUMON TANGTHIRASUNUN ◽  
PHILIPPE SILAR ◽  
DARBHE JAYARAMA BHAT ◽  
EKACHAI CHUKEATIROTE ◽  
D.N. NALIN WIJAYAWARDENE ◽  
...  
Keyword(s):  
Rna Polymerase Ii ◽  
Ribosomal Dna ◽  
Phylogenetic Trees ◽  
Sequence Data ◽  
Large Subunit ◽  
Elongation Factor ◽  
Morphological Characteristics ◽  
Distinct Species ◽  
Small Subunit ◽  
Family Status

Pseudorobillarda is a coelomycete genus of Dothideomycetes with appendaged conidia and 15 species epithets. In this study, we isolated four strains of Pseudorobillarda from dead leaves in Thailand. DNA sequence data generated from the large subunit (28S) ribosomal DNA (nuLSU) gene was used in phylogenetic studies. The phylogenetic trees generated indicate that Pseudorobillarda form a distinct lineage in Dothideomycetes that may eventually require separate family status. The Pseudorobillarda strains comprised two distinct species and this is also supported by morphological characteristics. In this paper we introduce a new species of Pseudorobillarda, P. eucalypti and compare it with other species of the genus. We also deposit data from the internal transcribed spacer (ITS), small subunit (18S) ribosomal DNA (nuSSU),the RNA polymerase II 2nd largest subunit genes (RPB2), Translation Elongation Factor 1-α (TEF1-α) and β-Tubulin (Bt) genes from each strain in GenBank for future studies.

Description of Ambrosiozyma oregonensis sp. nov., and reassignment of Candida species of the Ambrosiozyma clade to Ambrosiozyma kashinagacola f.a., comb. nov., Ambrosiozyma llanquihuensis f.a., comb. nov., Ambrosiozyma maleeae f.a., comb. nov., Ambrosiozyma pseudovanderkliftii f.a., comb. nov., and Ambrosiozyma vanderkliftii f.a., comb. nov.

2013 ◽  
Vol 63 (Pt_10) ◽  
pp. 3877-3883 ◽  
Author(s):  
Cletus P. Kurtzman ◽  
Christie J. Robnett
Keyword(s):  
Rna Polymerase Ii ◽  
Large Subunit ◽  
Elongation Factor ◽  
Nuclear Gene ◽  
Small Subunit ◽  
Mountain Stream ◽  
Small Subunit Rrna ◽  
Translation Elongation ◽  
Phenotypic Characters ◽  
Large Subunit Rrna

Ambrosiozyma oregonensis sp. nov. is described from two strains, one isolated from a mountain stream in Oregon, USA (NRRL Y-6106T = CBS 5560T), and a second (NRRL YB-4169) from an unknown substrate from Marion, Illinois, USA. The species forms four hat-shaped ascospores in each deliquescent ascus and appears to be homothallic. Abundant true hyphae are produced with some having apparent dolipore-like septa. Analyses of nuclear gene sequences for the D1/D2 domains of large-subunit rRNA, small-subunit rRNA, translation elongation factor-1α, and subunits B1 and B2 of RNA polymerase II show the proposed novel species to be distinct from other species of the Ambrosiozyma clade. Because of their placement in the Ambrosiozyma clade, Candida kashinagacola, Candida llanquihuensis, Candida maleeae, Candida pseudovanderkliftii and Candida vanderkliftii are reassigned to the genus Ambrosiozyma as new combinations, and the description of the genus Ambrosiozyma is emended to reflect the resulting changes in phenotypic characters.

scholarly journals Two pleosporalean root-colonizing fungi, Fuscosphaeria hungarica gen. et sp. nov. and Delitschia chaetomioides, from a semiarid grassland in Hungary

2021 ◽  
Vol 20 (1) ◽  
pp. 39-50
Author(s):  
Alexandra Pintye ◽  
Dániel G. Knapp
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Culture Media ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Elongation Factor ◽  
Single Species ◽  
Small Subunit ◽  
Grass Species ◽  
Plant Materials

AbstractIn this study, we investigated two unidentified lineages of root-colonizing fungi belonging to the order Pleosporales (Dothideomycetes), which were isolated from Festuca vaginata (Poaceae), a dominant grass species in the semiarid sandy grasslands of Hungary. For molecular phylogenetic studies, seven loci (internal transcribed spacer, partial large subunit and small subunit region of nrRNA, partial transcription elongation factor 1-α, RNA polymerase II largest subunit, RNA polymerase II second largest subunit, and ß-tubulin genes) were amplified and sequenced. Based on morphology and multilocus phylogenetic analyses, we found that one lineage belonged to Delitschia chaetomioides P. Karst. (Delitschiaceae), and the isolates of the other lineage represented a novel monotypic genus in the family Trematosphaeriaceae (suborder Massarineae). For this lineage, we proposed a new genus, Fuscosphaeria, represented by a single species, F. hungarica. In both lineages, only immature and degenerated sporocarps could be induced. These were sterile, black, globose, or depressed globose structures with numerous mycelioid appendages submerged in culture media or on the surface of autoclaved plant materials. Both species are first reported here as root-colonizing fungi.

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